diff --git a/modules/cnvkit/antitarget/main.nf b/modules/cnvkit/antitarget/main.nf
new file mode 100644
index 00000000..bf6461bd
--- /dev/null
+++ b/modules/cnvkit/antitarget/main.nf
@@ -0,0 +1,36 @@
+process CNVKIT_ANTITARGET {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda (params.enable_conda ? "bioconda::cnvkit=0.9.9" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/cnvkit:0.9.9--pyhdfd78af_0':
+        'quay.io/biocontainers/cnvkit:0.9.9--pyhdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(targets)
+
+    output:
+    tuple val(meta), path("*.bed"), emit: bed
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    """
+    cnvkit.py \\
+        antitarget \\
+        $targets \\
+        --output ${prefix}.antitarget.bed \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        cnvkit: \$(cnvkit.py version | sed -e "s/cnvkit v//g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/cnvkit/antitarget/meta.yml b/modules/cnvkit/antitarget/meta.yml
new file mode 100644
index 00000000..28a2bfa4
--- /dev/null
+++ b/modules/cnvkit/antitarget/meta.yml
@@ -0,0 +1,44 @@
+name: cnvkit_antitarget
+description:
+keywords:
+  - cvnkit
+  - antitarget
+tools:
+  - cnvkit:
+      description: |
+        CNVkit is a Python library and command-line software toolkit to infer and visualize copy number from high-throughput DNA sequencing data.
+        It is designed for use with hybrid capture, including both whole-exome and custom target panels, and short-read sequencing platforms such as Illumina and Ion Torrent.
+      homepage: https://cnvkit.readthedocs.io/en/stable/index.html
+      documentation: https://cnvkit.readthedocs.io/en/stable/index.html
+      tool_dev_url: "https://github.com/etal/cnvkit"
+      doi: 10.1371/journal.pcbi.1004873
+      licence: ["Apache-2.0"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - targets:
+      type: file
+      description: File containing genomic regions
+      pattern: "*.{bed}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bed:
+      type: file
+      description: File containing off-target regions
+      pattern: "*.{bed}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@SusiJo"
diff --git a/tests/config/pytest_modules.yml b/tests/config/pytest_modules.yml
index 78e85487..2131445c 100644
--- a/tests/config/pytest_modules.yml
+++ b/tests/config/pytest_modules.yml
@@ -447,6 +447,10 @@ cmseq/polymut:
   - modules/cmseq/polymut/**
   - tests/modules/cmseq/polymut/**
 
+cnvkit/antitarget:
+  - modules/cnvkit/antitarget/**
+  - tests/modules/cnvkit/antitarget/**
+
 cnvkit/batch:
   - modules/cnvkit/batch/**
   - tests/modules/cnvkit/batch/**
diff --git a/tests/modules/cnvkit/antitarget/main.nf b/tests/modules/cnvkit/antitarget/main.nf
new file mode 100644
index 00000000..44e49eb2
--- /dev/null
+++ b/tests/modules/cnvkit/antitarget/main.nf
@@ -0,0 +1,16 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { CNVKIT_ANTITARGET } from '../../../../modules/cnvkit/antitarget/main.nf'
+
+workflow test_cnvkit_antitarget {
+
+    input = [
+        [ id:'test' ], // meta map
+        file(params.test_data['homo_sapiens']['genome']['genome_21_multi_interval_bed'], checkIfExists: true)
+    ]
+
+    CNVKIT_ANTITARGET ( input )
+}
+
diff --git a/tests/modules/cnvkit/antitarget/nextflow.config b/tests/modules/cnvkit/antitarget/nextflow.config
new file mode 100644
index 00000000..50f50a7a
--- /dev/null
+++ b/tests/modules/cnvkit/antitarget/nextflow.config
@@ -0,0 +1,5 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+    
+}
\ No newline at end of file
diff --git a/tests/modules/cnvkit/antitarget/test.yml b/tests/modules/cnvkit/antitarget/test.yml
new file mode 100644
index 00000000..e1c3d0c8
--- /dev/null
+++ b/tests/modules/cnvkit/antitarget/test.yml
@@ -0,0 +1,8 @@
+- name: cnvkit antitarget test_cnvkit_antitarget
+  command: nextflow run ./tests/modules/cnvkit/antitarget -entry test_cnvkit_antitarget -c ./tests/config/nextflow.config  -c ./tests/modules/cnvkit/antitarget/nextflow.config
+  tags:
+    - cnvkit
+    - cnvkit/antitarget
+  files:
+    - path: output/cnvkit/test.antitarget.bed
+      md5sum: 3d4d20f9f23b39970865d29ef239d20b