diff --git a/tests/software/preseq/main.nf b/tests/software/preseq/main.nf
index dee9b2d6..2091dcc6 100644
--- a/tests/software/preseq/main.nf
+++ b/tests/software/preseq/main.nf
@@ -13,7 +13,7 @@ workflow test_preseq_single_end {
 
     def input = []
     input = [ [ id:'test', single_end:true ], // meta map
-              [ file("${launchDir}/tests/data/bed/A.bed", checkIfExists: true) ]]
+              [ file('https://github.com/smithlabcode/preseq/raw/master/data/SRR1003759_5M_subset.mr', checkIfExists: true), ] ]
     PRESEQ_LCEXTRAP_SE ( input )
 }
 
@@ -25,7 +25,7 @@ workflow test_preseq_paired_end {
 
     def input = []
     input = [ [ id:'test', single_end:false ], // meta map
-              [ file("${launchDir}/tests/data/bed/A.bed", checkIfExists: true) ]]
+              [ file('https://github.com/smithlabcode/preseq/raw/master/data/SRR1003759_5M_subset.mr', checkIfExists: true), ] ]
 
     PRESEQ_LCEXTRAP_PE ( input )
 }
diff --git a/tests/software/preseq/test.yml b/tests/software/preseq/test.yml
index b8381438..14d940da 100644
--- a/tests/software/preseq/test.yml
+++ b/tests/software/preseq/test.yml
@@ -6,7 +6,7 @@
 
   files:
     - path: output/test_preseq_single_end/test.ccurve.txt
-      md5sum: 5253ffcfd4b6162851631efdbfe16f92
+      md5sum: 76ae04c8eaf19c94e3210bb69da38498
     - path: output/test_preseq_single_end/test.command.log
 
 - name: Run preseq paired-end lcextrap
@@ -17,5 +17,5 @@
 
   files:
     - path: output/test_preseq_paired_end/test.ccurve.txt
-      md5sum: 66b339780630fc1aa72dcfbd0a4490fd
+      md5sum: 2836d2fabd2213f097fd7063db550276
     - path: output/test_preseq_paired_end/test.command.log