Add MerquryFK PloidyPlot (#1780)

modules/merquryfk/ploidyplot/main.nf

@@ -0,0 +1,44 @@
+process MERQURYFK_PLOIDYPLOT {
+    tag "$meta.id"
+    label 'process_medium'
+
+    if (params.enable_conda) {
+        error "Conda environments cannot be used when using the FastK tool. Please use docker or singularity containers."
+    }
+    container 'ghcr.io/nbisweden/fastk_genescopefk_merquryfk:1.0'
+
+    input:
+    tuple val(meta), path(fastk_hist), path(fastk_ktab)
+
+    output:
+    tuple val(meta), path("*.fi.png"), emit: filled_plodiy_plot_png , optional: true
+    tuple val(meta), path("*.fi.pdf"), emit: filled_plodiy_plot_pdf , optional: true
+    tuple val(meta), path("*.ln.png"), emit: line_plodiy_plot_png   , optional: true
+    tuple val(meta), path("*.ln.pdf"), emit: line_plodiy_plot_pdf   , optional: true
+    tuple val(meta), path("*.st.png"), emit: stacked_plodiy_plot_png, optional: true
+    tuple val(meta), path("*.st.pdf"), emit: stacked_plodiy_plot_pdf, optional: true
+    path "versions.yml"              , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def FASTK_VERSION = 'f18a4e6d2207539f7b84461daebc54530a9559b0'
+    def MERQURY_VERSION = '8f3ab706e4cf4d7b7d1dfe5739859e3ebd26c494'
+    """
+    PloidyPlot \\
+        $args \\
+        -T$task.cpus \\
+        -o$prefix \\
+        ${fastk_ktab.find{ it.toString().endsWith(".ktab") }}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastk: $FASTK_VERSION
+        merquryfk: $MERQURY_VERSION
+        r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' )
+    END_VERSIONS
+    """
+}

modules/merquryfk/ploidyplot/meta.yml

@@ -0,0 +1,67 @@
+name: "merquryfk_ploidyplot"
+description: An improved version of Smudgeplot using FastK
+keywords:
+  - kmer
+  - smudgeplot
+  - ploidy
+tools:
+  - "merquryfk":
+      description: "FastK based version of Merqury"
+      homepage: "https://github.com/thegenemyers/MERQURY.FK"
+      documentation: ""
+      tool_dev_url: "https://github.com/thegenemyers/MERQURY.FK"
+      doi: ""
+      licence: "https://github.com/thegenemyers/MERQURY.FK/blob/main/LICENSE"
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - fastk_hist:
+      type: file
+      description: A histogram files from the program FastK
+      pattern: "*.hist"
+  - fastk_ktab:
+      type: file
+      description: ktab files from the program FastK
+      pattern: "*.ktab*"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - filled_ploidy_plot_png:
+      type: file
+      description: A filled ploidy plot in PNG format
+      pattern: "*.fi.png"
+  - filled_ploidy_plot_pdf:
+      type: file
+      description: A filled ploidy plot in PDF format
+      pattern: "*.fi.pdf"
+  - line_ploidy_plot_png:
+      type: file
+      description: A line ploidy plot in PNG format
+      pattern: "*.ln.png"
+  - line_ploidy_plot_pdf:
+      type: file
+      description: A lin4 ploidy plot in PDF format
+      pattern: "*.ln.pdf"
+  - stacked_ploidy_plot_png:
+      type: file
+      description: A stacked ploidy plot in PNG format
+      pattern: "*.st.png"
+  - stacked_ploidy_plot_pdf:
+      type: file
+      description: A stacked ploidy plot in PDF format
+      pattern: "*.st.pdf"
+
+authors:
+  - "@mahesh-panchal"

tests/config/pytest_modules.yml

@@ -1338,6 +1338,10 @@ merquryfk/merquryfk:
   - modules/merquryfk/merquryfk/**
   - tests/modules/merquryfk/merquryfk/**
 
+merquryfk/ploidyplot:
+  - modules/merquryfk/ploidyplot/**
+  - tests/modules/merquryfk/ploidyplot/**
+
 meryl/count:
   - modules/meryl/count/**
   - tests/modules/meryl/count/**

tests/modules/merquryfk/ploidyplot/main.nf

@@ -0,0 +1,33 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { FASTK_FASTK          } from '../../../../modules/fastk/fastk/main.nf'
+include { MERQURYFK_PLOIDYPLOT } from '../../../../modules/merquryfk/ploidyplot/main.nf'
+
+workflow test_merquryfk_ploidyplot_png {
+
+    input = [
+        [ id:'test', single_end:true ], // meta map
+        file(params.test_data['homo_sapiens']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+    ]
+
+    FASTK_FASTK ( input )
+    MERQURYFK_PLOIDYPLOT ( FASTK_FASTK.out.hist
+        .join( FASTK_FASTK.out.ktab )
+    )
+}
+
+workflow test_merquryfk_ploidyplot_pdf {
+
+    input = [
+        [ id:'test', single_end:true ], // meta map
+        file(params.test_data['homo_sapiens']['illumina']['test_1_fastq_gz'], checkIfExists: true)
+    ]
+
+    FASTK_FASTK ( input )
+    MERQURYFK_PLOIDYPLOT (
+        FASTK_FASTK.out.hist
+            .join( FASTK_FASTK.out.ktab )
+    )
+}

tests/modules/merquryfk/ploidyplot/nextflow.config

@@ -0,0 +1,13 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+
+    withName: 'FASTK_.*' {
+        ext.args   = '-t'
+        publishDir = [ enabled: false ]
+    }
+    withName: 'test_merquryfk_ploidyplot_pdf:MERQURYFK_PLOIDYPLOT' {
+        ext.args   = '-pdf'
+    }
+
+}

tests/modules/merquryfk/ploidyplot/test.yml

@@ -0,0 +1,22 @@
+- name: merquryfk ploidyplot test_merquryfk_ploidyplot_png
+  command: nextflow run ./tests/modules/merquryfk/ploidyplot -entry test_merquryfk_ploidyplot_png -c ./tests/config/nextflow.config  -c ./tests/modules/merquryfk/ploidyplot/nextflow.config
+  tags:
+    - merquryfk
+    - merquryfk/ploidyplot
+  files:
+    - path: output/merquryfk/test.fi.png
+      md5sum: c6f883f3d57ad64727219052e97d39b3
+    - path: output/merquryfk/test.ln.png
+      md5sum: bd299e2eceb258328d1a41c024d7fbf4
+    - path: output/merquryfk/test.st.png
+      md5sum: 4f858ed9712196606859675c3f3ba7a9
+
+- name: merquryfk ploidyplot test_merquryfk_ploidyplot_pdf
+  command: nextflow run ./tests/modules/merquryfk/ploidyplot -entry test_merquryfk_ploidyplot_pdf -c ./tests/config/nextflow.config  -c ./tests/modules/merquryfk/ploidyplot/nextflow.config
+  tags:
+    - merquryfk
+    - merquryfk/ploidyplot
+  files:
+    - path: output/merquryfk/test.fi.pdf
+    - path: output/merquryfk/test.ln.pdf
+    - path: output/merquryfk/test.st.pdf