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Bedtools genomecov adapted to the current module standard
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6 changed files with 22 additions and 8 deletions
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@ -4,4 +4,4 @@ LABEL authors="Jose Espinosa-Carrasco" \
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COPY environment.yml /
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RUN conda env create -f /environment.yml && conda clean -a
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ENV PATH /opt/conda/envs/nf-core-bedtools-complementbed/bin:$PATH
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ENV PATH /opt/conda/envs/nf-core-bedtools-genomecov/bin:$PATH
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@ -1,18 +1,33 @@
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def MODULE = "bedtools_genomecov"
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params.publish_dir = MODULE
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params.publish_results = "default"
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process BEDTOOLS_GENOMECOV {
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tag {bam}
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publishDir "${params.out_dir}/${params.publish_dir}",
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mode: params.publish_dir_mode,
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saveAs: { filename ->
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if (params.publish_results == "none") null
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else filename }
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//container "docker.pkg.github.com/nf-core/$MODULE"
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container 'quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0'
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conda "${moduleDir}/environment.yml"
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input:
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path (bam)
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path (chrom_sizes)
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val (bedtools_genomecov_args)
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output:
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stdout()
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path "${bam}.bed", emit: coverage
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path "*.version.txt", emit: version
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script:
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"""
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bedtools genomecov -ibam ${bam} -g ${chrom_sizes} ${bedtools_genomecov_args}
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bedtools genomecov -ibam ${bam} -g ${chrom_sizes} ${bedtools_genomecov_args} > ${bam}.bed
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bedtools --version | sed -n "s/.*\\(v.*\$\\)/\\1/p" > bedtools.version.txt
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"""
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}
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Binary file not shown.
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@ -0,0 +1 @@
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../../../../../tests/data/bam/JK2067_downsampled_s0.1.bam
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@ -1 +0,0 @@
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chr1 1780869
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1
software/bedtools/genomecov/test/input_data/genome.sizes
Symbolic link
1
software/bedtools/genomecov/test/input_data/genome.sizes
Symbolic link
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@ -0,0 +1 @@
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../../../../../tests/data/bed/genome.sizes
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@ -2,9 +2,11 @@
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nextflow.preview.dsl = 2
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params.out_dir = "test_output"
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params.fastqc_args = ''
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params.publish_dir_mode = "copy"
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params.bedtools_genomecov_args = '' //'-bg'
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include check_output from '../../../../tests/functions/check_process_outputs.nf' // params(params)
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include BEDTOOLS_GENOMECOV from '../main.nf' params(params)
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// Define input channels
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@ -14,5 +16,4 @@ chrom_sizes = Channel.fromPath('./input_data/genome.sizes')
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// Run the workflow
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workflow {
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BEDTOOLS_GENOMECOV(ch_input, chrom_sizes, params.bedtools_genomecov_args)
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// .check_output()
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}
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@ -1,2 +0,0 @@
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docker.enabled = true
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params.outdir = './results'
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