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https://github.com/MillironX/nf-core_modules.git
synced 2024-11-13 05:13:09 +00:00
refactor: require NCBI user settings always
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parent
eae7161a32
commit
0cdf7767a7
6 changed files with 12 additions and 104 deletions
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@ -9,7 +9,7 @@ process SRATOOLS_FASTERQDUMP {
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input:
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tuple val(meta), path(sra)
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path(ncbi_settings)
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path ncbi_settings
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output:
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tuple val(meta), path(output), emit: reads
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@ -26,9 +26,7 @@ process SRATOOLS_FASTERQDUMP {
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// for unpaired reads which we ignore here.
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output = meta.single_end ? '*.fastq.gz' : '*_{1,2}.fastq.gz'
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"""
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if [[ "${ncbi_settings.name}" != "EXISTS" ]]; then
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export NCBI_SETTINGS="\$PWD/${ncbi_settings}"
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fi
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export NCBI_SETTINGS="\$PWD/${ncbi_settings}"
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fasterq-dump \\
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$args \\
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@ -25,9 +25,8 @@ input:
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- ncbi_settings:
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type: file
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description: >
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Either a proper NCBI settings file or in case an existing file should be used,
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a file with name EXISTS.
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pattern: "*.mkfg | EXISTS"
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An NCBI user settings file.
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pattern: "*.mkfg"
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output:
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- meta:
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@ -5,11 +5,7 @@ nextflow.enable.dsl = 2
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include { UNTAR } from '../../../../modules/untar/main.nf'
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include { SRATOOLS_FASTERQDUMP } from '../../../../modules/sratools/fasterqdump/main.nf'
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workflow test_sratools_fasterqdump_single_end_with_input {
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file(params.settings_path).mkdirs()
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def settings = file(params.settings_file)
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settings.text = "/LIBS/GUID = \"5b0d4b7d-88c7-4802-98fd-e3afd06feb32\"\n/libs/cloud/report_instance_identity = \"true\"\n"
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workflow test_sratools_fasterqdump_single_end {
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archive = [ [], file(params.test_data['sarscov2']['illumina']['SRR13255544_tar_gz'], checkIfExists: true) ]
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UNTAR ( archive )
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@ -17,14 +13,10 @@ workflow test_sratools_fasterqdump_single_end_with_input {
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def input = Channel.of([ id:'test_single_end', single_end:true ])
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.combine(UNTAR.out.untar.map{ it[1] })
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SRATOOLS_FASTERQDUMP(input, settings)
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SRATOOLS_FASTERQDUMP(input, file(params.test_data['generic']['config']['ncbi_user_settings'], checkIfExists: true))
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}
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workflow test_sratools_fasterqdump_paired_end_with_input {
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file(params.settings_path).mkdirs()
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def settings = file(params.settings_file)
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settings.text = "/LIBS/GUID = \"5b0d4b7d-88c7-4802-98fd-e3afd06feb32\"\n/libs/cloud/report_instance_identity = \"true\"\n"
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workflow test_sratools_fasterqdump_paired_end {
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archive = [ [], file(params.test_data['sarscov2']['illumina']['SRR11140744_tar_gz'], checkIfExists: true) ]
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UNTAR ( archive )
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@ -32,35 +24,5 @@ workflow test_sratools_fasterqdump_paired_end_with_input {
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def input = Channel.of([ id:'test_paired_end', single_end:false ])
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.combine(UNTAR.out.untar.map{ it[1] })
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SRATOOLS_FASTERQDUMP(input, settings)
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}
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workflow test_sratools_fasterqdump_single_end_without_input {
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file(params.settings_path).mkdirs()
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def settings = file(params.settings_file)
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settings.text = "/LIBS/GUID = \"5b0d4b7d-88c7-4802-98fd-e3afd06feb32\"\n/libs/cloud/report_instance_identity = \"true\"\n"
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archive = [ [], file(params.test_data['sarscov2']['illumina']['SRR13255544_tar_gz'], checkIfExists: true) ]
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UNTAR ( archive )
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def input = Channel.of([ id:'test_single_end', single_end:true ])
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.combine(UNTAR.out.untar.map{ it[1] })
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SRATOOLS_FASTERQDUMP(input, file('EXISTS'))
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}
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workflow test_sratools_fasterqdump_paired_end_without_input {
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file(params.settings_path).mkdirs()
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def settings = file(params.settings_file)
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settings.text = "/LIBS/GUID = \"5b0d4b7d-88c7-4802-98fd-e3afd06feb32\"\n/libs/cloud/report_instance_identity = \"true\"\n"
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archive = [ [], file(params.test_data['sarscov2']['illumina']['SRR11140744_tar_gz'], checkIfExists: true) ]
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UNTAR ( archive )
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def input = Channel.of([ id:'test_paired_end', single_end:false ])
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.combine(UNTAR.out.untar.map{ it[1] })
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SRATOOLS_FASTERQDUMP(input, file('EXISTS'))
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SRATOOLS_FASTERQDUMP(input, file(params.test_data['generic']['config']['ncbi_user_settings'], checkIfExists: true))
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}
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@ -1,6 +1,3 @@
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params.settings_path = '/tmp/.ncbi'
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params.settings_file = "${params.settings_path}/user-settings.mkfg"
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process {
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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@ -1,18 +0,0 @@
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params.settings_path = '/tmp/.ncbi'
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params.settings_file = "${params.settings_path}/user-settings.mkfg"
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env.NCBI_SETTINGS = params.settings_file
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process {
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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withName: SRATOOLS_FASTERQDUMP {
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containerOptions = {
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(workflow.containerEngine == 'singularity') ?
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"-B ${params.settings_path}:${params.settings_path}" :
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"-v ${params.settings_path}:${params.settings_path}"
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}
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}
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}
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@ -1,5 +1,5 @@
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- name: sratools fasterqdump test_sratools_fasterqdump_single_end_with_input
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command: nextflow run ./tests/modules/sratools/fasterqdump -entry test_sratools_fasterqdump_single_end_with_input -c ./tests/config/nextflow.config -c ./tests/modules/sratools/fasterqdump/nextflow.config
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- name: sratools fasterqdump test_sratools_fasterqdump_single_end
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command: nextflow run ./tests/modules/sratools/fasterqdump -entry test_sratools_fasterqdump_single_end -c ./tests/config/nextflow.config -c ./tests/modules/sratools/fasterqdump/nextflow.config
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tags:
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- sratools
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- sratools/fasterqdump
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@ -12,38 +12,8 @@
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contains:
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- "sratools: 2.11.0"
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- name: sratools fasterqdump test_sratools_fasterqdump_paired_end_with_input
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command: nextflow run ./tests/modules/sratools/fasterqdump -entry test_sratools_fasterqdump_paired_end_with_input -c ./tests/config/nextflow.config -c ./tests/modules/sratools/fasterqdump/nextflow.config
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tags:
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- sratools
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- sratools/fasterqdump
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files:
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- path: output/sratools/SRR11140744_1.fastq.gz
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md5sum: 193809c784a4ea132ab2a253fa4f55b6
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- path: output/sratools/SRR11140744_2.fastq.gz
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md5sum: 3e3b3af3413f50a1685fd7b3f1456d4e
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- path: output/untar/SRR11140744/SRR11140744.sra
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md5sum: 065666caf5b2d5dfb0cb25d5f3abe659
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- path: output/sratools/versions.yml
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contains:
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- "sratools: 2.11.0"
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- name: sratools fasterqdump test_sratools_fasterqdump_single_end_without_input
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command: nextflow run ./tests/modules/sratools/fasterqdump -entry test_sratools_fasterqdump_single_end_without_input -c ./tests/config/nextflow.config -c ./tests/modules/sratools/fasterqdump/nextflow_mount.config
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tags:
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- sratools
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- sratools/fasterqdump
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files:
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- path: output/sratools/SRR13255544.fastq.gz
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md5sum: 1054c7b71884acdb5eed8a378f18be82
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- path: output/untar/SRR13255544/SRR13255544.sra
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md5sum: 466d05dafb2eec672150754168010b4d
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- path: output/sratools/versions.yml
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contains:
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- "sratools: 2.11.0"
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- name: sratools fasterqdump test_sratools_fasterqdump_paired_end_without_input
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command: nextflow run ./tests/modules/sratools/fasterqdump -entry test_sratools_fasterqdump_paired_end_without_input -c ./tests/config/nextflow.config -c ./tests/modules/sratools/fasterqdump/nextflow_mount.config
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- name: sratools fasterqdump test_sratools_fasterqdump_paired_end
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command: nextflow run ./tests/modules/sratools/fasterqdump -entry test_sratools_fasterqdump_paired_end -c ./tests/config/nextflow.config -c ./tests/modules/sratools/fasterqdump/nextflow.config
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tags:
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- sratools
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- sratools/fasterqdump
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