diff --git a/tests/modules/cat/fastq/main.nf b/tests/modules/cat/fastq/main.nf
index 1ed23ce5..b8c739e4 100644
--- a/tests/modules/cat/fastq/main.nf
+++ b/tests/modules/cat/fastq/main.nf
@@ -47,3 +47,12 @@ workflow test_cat_fastq_paired_end_same_name {
 
     CAT_FASTQ ( input )
 }
+
+workflow test_cat_fastq_single_end_single_file {
+    input = [
+        [ id:'test', single_end: true ],
+        [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
+    ]
+
+    CAT_FASTQ( input )
+}
diff --git a/tests/modules/cat/fastq/test.yml b/tests/modules/cat/fastq/test.yml
index 56374060..d9fe33e2 100644
--- a/tests/modules/cat/fastq/test.yml
+++ b/tests/modules/cat/fastq/test.yml
@@ -37,3 +37,12 @@
       md5sum: 63f817db7a29a03eb538104495556f66
     - path: ./output/cat/test_2.merged.fastq.gz
       md5sum: fe9f266f43a6fc3dcab690a18419a56e
+
+- name: cat fastq single-end-single-file
+  command: nextflow run ./tests/modules/cat/fastq -entry test_cat_fastq_single_end_single_file -c ./tests/config/nextflow.config -c ./tests/modules/cat/fastq/nextflow.config
+  tags:
+    - cat
+    - cat/fastq
+  files:
+    - path: ./output/cat/test.merged.fastq.gz
+      md5sum: e325ef7deb4023447a1f074e285761af