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add input database and intermediate bam file.
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3 changed files with 20 additions and 5 deletions
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@ -9,6 +9,8 @@ process MOTUS_PROFILE {
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input:
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tuple val(meta), path(reads)
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path db
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path bam
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output:
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tuple val(meta), path("*.out"), emit: out
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@ -25,12 +27,16 @@ process MOTUS_PROFILE {
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"-i ${reads}" :
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meta.single_end ?
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"-s $reads" : "-f ${reads[0]} -r ${reads[1]}"
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def refdb = db ? "-db ${db}" : ""
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def intermediateBam = bam ? "-I $bam" : ""
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"""
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motus profile \\
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$args \\
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$inputs \\
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$refdb \\
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$intermediateBam \\
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-t $task.cpus \\
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-n ${prefix} \\
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-n $prefix \\
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-o ${prefix}.out
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cat <<-END_VERSIONS > versions.yml
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@ -14,7 +14,6 @@ tools:
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doi: "10.1038/s41467-019-08844-4"
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licence: "['GPL v3']"
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## TODO nf-core: Add a description of all of the variables used as input
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input:
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- meta:
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type: map
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@ -31,6 +30,11 @@ input:
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type: derectory
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description: |
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mOTUs database downloaded by `motus downloadDB`
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- bam:
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type: file
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description: |
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The intermediate bam file mapped by bwa to the mOTUs database or
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the output bam file from motus profile
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output:
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- meta:
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@ -2,7 +2,8 @@
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nextflow.enable.dsl = 2
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include { MOTUS_PROFILE } from '../../../../modules/motus/profile/main.nf'
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include { MOTUS_DOWNLOADDB } from '../../../../modules/motus/downloaddb/main.nf'
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include { MOTUS_PROFILE } from '../../../../modules/motus/profile/main.nf'
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workflow test_motus_profile_single_end {
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@ -11,7 +12,9 @@ workflow test_motus_profile_single_end {
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file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
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]
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MOTUS_PROFILE ( input )
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MOTUS_DOWNLOADDB(file('https://raw.githubusercontent.com/motu-tool/mOTUs/master/motus/downloadDB.py'))
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MOTUS_PROFILE ( input, MOTUS_DOWNLOADDB.out.db, [])
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}
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workflow test_motus_profile_paired_end {
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@ -21,6 +24,8 @@ workflow test_motus_profile_paired_end {
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[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
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file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
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]
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MOTUS_PROFILE ( input )
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MOTUS_DOWNLOADDB(file('https://raw.githubusercontent.com/motu-tool/mOTUs/master/motus/downloadDB.py'))
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MOTUS_PROFILE ( input, MOTUS_DOWNLOADDB.out.db, [])
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}
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