Update Salmon quant to use BAM input

software/salmon/quant/main.nf

@@ -18,6 +18,8 @@ process SALMON_QUANT {
     tuple val(meta), path(reads)
     path  index
     path  gtf
+    path  transcript_fasta
+    val   alignment_mode
     
     output:
     tuple val(meta), path("${prefix}"), emit: results
@@ -26,7 +28,13 @@ process SALMON_QUANT {
     script:
     def software    = getSoftwareName(task.process)
     prefix          = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
-    def endedness = meta.single_end ? "-r $reads" : "-1 ${reads[0]} -2 ${reads[1]}"
+
+    def reference   = "--index $index"
+    def input_reads = meta.single_end ? "-r $reads" : "-1 ${reads[0]} -2 ${reads[1]}"
+    if (alignment_mode) {
+        reference   = "-t $transcript_fasta"
+        input_reads = "-a $reads"
+    }
     
     def strandedness = meta.single_end ? 'U' : 'IU'
     if (meta.strandedness == 'forward') {
@@ -39,8 +47,8 @@ process SALMON_QUANT {
         --geneMap $gtf \\
         --threads $task.cpus \\
         --libType=$strandedness \\
-        --index $index \\
-        $endedness \\
+        $reference \\
+        $input_reads \\
         $options.args \\
         -o $prefix