Add meryl histogram

This commit is contained in:
Mahesh Binzer-Panchal 2022-05-09 08:50:28 +00:00
parent 38d4f81d20
commit 129f57ccd9
6 changed files with 119 additions and 4 deletions

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@ -0,0 +1,34 @@
process MERYL_HISTOGRAM {
tag "$meta.id"
label 'process_low'
conda (params.enable_conda ? "bioconda::meryl=1.3" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/meryl:1.3--h87f3376_1':
'quay.io/biocontainers/meryl:1.3--h87f3376_1' }"
input:
tuple val(meta), path(meryl_db)
output:
tuple val(meta), path("*.hist"), emit: hist
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
"""
meryl histogram \\
threads=$task.cpus \\
$args \\
$meryl_db > ${prefix}.hist
cat <<-END_VERSIONS > versions.yml
"${task.process}":
meryl: \$( meryl --version |& sed 's/meryl //' )
END_VERSIONS
"""
}

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name: "meryl_histogram"
description: A genomic k-mer counter (and sequence utility) with nice features.
keywords:
- k-mer
- histogram
tools:
- "meryl":
description: "A genomic k-mer counter (and sequence utility) with nice features. "
homepage: "https://github.com/marbl/meryl"
documentation: "https://meryl.readthedocs.io/en/latest/quick-start.html"
tool_dev_url: "https://github.com/marbl/meryl"
doi: ""
licence: "['GPL']"
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- meryl_dbs:
type: directory
description: Meryl k-mer database
output:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
- hist:
type: file
description: Histogram of k-mers
pattern: "*.hist"
authors:
- "@mahesh-panchal"

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@ -1214,6 +1214,10 @@ meningotype:
- modules/meningotype/** - modules/meningotype/**
- tests/modules/meningotype/** - tests/modules/meningotype/**
meryl/histogram:
- modules/meryl/histogram/**
- tests/modules/meryl/histogram/**
metabat2/jgisummarizebamcontigdepths: metabat2/jgisummarizebamcontigdepths:
- modules/metabat2/jgisummarizebamcontigdepths/** - modules/metabat2/jgisummarizebamcontigdepths/**
- tests/modules/metabat2/jgisummarizebamcontigdepths/** - tests/modules/metabat2/jgisummarizebamcontigdepths/**
@ -1763,14 +1767,14 @@ slimfastq:
- modules/slimfastq/** - modules/slimfastq/**
- tests/modules/slimfastq/** - tests/modules/slimfastq/**
snapaligner/index:
- modules/snapaligner/index/**
- tests/modules/snapaligner/index/**
snapaligner/align: snapaligner/align:
- modules/snapaligner/align/** - modules/snapaligner/align/**
- tests/modules/snapaligner/align/** - tests/modules/snapaligner/align/**
snapaligner/index:
- modules/snapaligner/index/**
- tests/modules/snapaligner/index/**
snpdists: snpdists:
- modules/snpdists/** - modules/snpdists/**
- tests/modules/snpdists/** - tests/modules/snpdists/**

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#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
include { MERYL_COUNT } from '../../../../modules/meryl/count/main.nf'
include { MERYL_HISTOGRAM } from '../../../../modules/meryl/histogram/main.nf'
workflow test_meryl_histogram {
input = [
[ id:'test' ], // meta map
file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
]
MERYL_COUNT ( input )
MERYL_HISTOGRAM ( MERYL_COUNT.out.meryl_db )
}

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process {
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
}

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## TODO nf-core: Please run the following command to build this file:
# nf-core modules create-test-yml meryl/histogram
- name: "meryl histogram"
command: nextflow run ./tests/modules/meryl/histogram -entry test_meryl_histogram -c ./tests/config/nextflow.config -c ./tests/modules/meryl/histogram/nextflow.config
tags:
- "meryl"
#
- "meryl/histogram"
#
files:
- path: "output/meryl/test.bam"
md5sum: e667c7caad0bc4b7ac383fd023c654fc
- path: output/meryl/versions.yml
md5sum: a01fe51bc4c6a3a6226fbf77b2c7cf3b