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feat: add module for slimfastq
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6 changed files with 189 additions and 0 deletions
52
modules/slimfastq/main.nf
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52
modules/slimfastq/main.nf
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def VERSION = '2.04'
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process SLIMFASTQ {
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tag "$meta.id"
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label 'process_low'
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conda (params.enable_conda ? "bioconda::slimfastq=2.04" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/slimfastq:2.04--h87f3376_2':
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'quay.io/biocontainers/slimfastq:2.04--h87f3376_2' }"
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input:
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tuple val(meta), path(fastq)
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output:
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tuple val(meta), path("*.sfq"), emit: sfq
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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if (meta.single_end) {
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"""
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gzip -d -c '${fastq}' | slimfastq \\
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$args \\
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-f '${prefix}.sfq'
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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slimfastq: ${VERSION}
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END_VERSIONS
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"""
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} else {
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"""
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gzip -d -c '${fastq[0]}' | slimfastq \\
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$args \\
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-f '${prefix}_1.sfq'
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gzip -d -c '${fastq[1]}' | slimfastq \\
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$args \\
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-f '${prefix}_2.sfq'
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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slimfastq: ${VERSION}
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END_VERSIONS
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"""
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}
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}
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41
modules/slimfastq/meta.yml
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41
modules/slimfastq/meta.yml
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name: "slimfastq"
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description: Fast, efficient, lossless compression of FASTQ files.
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keywords:
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- FASTQ
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- compression
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- lossless
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tools:
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- "slimfastq":
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description: "slimfastq efficiently compresses/decompresses FASTQ files"
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homepage: "https://github.com/Infinidat/slimfastq"
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tool_dev_url: "https://github.com/Infinidat/slimfastq"
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licence: "['BSD-3-clause']"
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input:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- fastq:
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type: file
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description: Either a single-end FASTQ file or paired-end files.
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pattern: "*.{fq.gz,fastq.gz}"
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output:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- versions:
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type: file
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description: File containing software versions
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pattern: "versions.yml"
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- sfq:
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type: file
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description: Either one or two sequence files in slimfastq compressed format.
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pattern: "*.{sfq}"
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authors:
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- "@Midnighter"
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@ -1727,6 +1727,10 @@ sistr:
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- modules/sistr/**
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- tests/modules/sistr/**
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slimfastq:
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- modules/slimfastq/**
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- tests/modules/slimfastq/**
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snapaligner/index:
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- modules/snapaligner/index/**
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- tests/modules/snapaligner/index/**
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46
tests/modules/slimfastq/main.nf
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46
tests/modules/slimfastq/main.nf
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#!/usr/bin/env nextflow
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nextflow.enable.dsl = 2
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include { SLIMFASTQ } from '../../../modules/slimfastq/main.nf'
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workflow test_slimfastq_single_end {
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input = [
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[ id:'test', single_end:true ], // meta map
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file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
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]
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SLIMFASTQ ( input )
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}
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workflow test_slimfastq_paired_end {
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input = [
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[ id:'test', single_end:false ], // meta map
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[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
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file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)]
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]
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SLIMFASTQ ( input )
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}
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workflow test_slimfastq_nanopore {
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input = [
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[ id:'test', single_end:true ], // meta map
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file(params.test_data['sarscov2']['nanopore']['test_fastq_gz'], checkIfExists: true)
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]
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SLIMFASTQ ( input )
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}
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workflow test_slimfastq_pacbio {
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input = [
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[ id:'test', single_end:true ], // meta map
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file(params.test_data['homo_sapiens']['pacbio']['ccs_fq_gz'], checkIfExists: true)
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]
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SLIMFASTQ ( input )
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}
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5
tests/modules/slimfastq/nextflow.config
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5
tests/modules/slimfastq/nextflow.config
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process {
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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}
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41
tests/modules/slimfastq/test.yml
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41
tests/modules/slimfastq/test.yml
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- name: slimfastq test_slimfastq_single_end
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command: nextflow run tests/modules/slimfastq -entry test_slimfastq_single_end -c tests/config/nextflow.config
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tags:
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- slimfastq
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files:
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- path: output/slimfastq/test.sfq
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md5sum: 6a942eeca6c99ee9a9a0cedab5d246f1
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- path: output/slimfastq/versions.yml
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md5sum: f52351f5c9e6259af02745c8eae5c780
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- name: slimfastq test_slimfastq_paired_end
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command: nextflow run tests/modules/slimfastq -entry test_slimfastq_paired_end -c tests/config/nextflow.config
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tags:
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- slimfastq
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files:
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- path: output/slimfastq/test_1.sfq
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md5sum: 6a942eeca6c99ee9a9a0cedab5d246f1
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- path: output/slimfastq/test_2.sfq
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md5sum: 0d2c60b52a39f7c2cb7843e848d90afd
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- path: output/slimfastq/versions.yml
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md5sum: 6239853705877651a4851c4cb6d62da4
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- name: slimfastq test_slimfastq_nanopore
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command: nextflow run tests/modules/slimfastq -entry test_slimfastq_nanopore -c tests/config/nextflow.config
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tags:
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- slimfastq
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files:
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- path: output/slimfastq/test.sfq
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md5sum: e17f14d64d3a75356b03ff2f9e8881f7
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- path: output/slimfastq/versions.yml
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md5sum: 33153f1103482a2bd35cb2f4c337c5e8
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- name: slimfastq test_slimfastq_pacbio
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command: nextflow run tests/modules/slimfastq -entry test_slimfastq_pacbio -c tests/config/nextflow.config
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tags:
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- slimfastq
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files:
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- path: output/slimfastq/test.sfq
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md5sum: 9e8389e47e6ddf8c25e92412dd628339
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- path: output/slimfastq/versions.yml
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md5sum: 1982789c3d5c7de37c0a9351e4ae63f7
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