diff --git a/software/nanoplot/functions.nf b/software/nanoplot/functions.nf new file mode 100644 index 00000000..d25eea86 --- /dev/null +++ b/software/nanoplot/functions.nf @@ -0,0 +1,59 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/software/nanoplot/main.nf b/software/nanoplot/main.nf new file mode 100644 index 00000000..176d1605 --- /dev/null +++ b/software/nanoplot/main.nf @@ -0,0 +1,42 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process NANOPLOT { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } + + conda (params.enable_conda ? "bioconda::nanoplot=1.32.1" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/nanoplot:1.32.1--py_0" + } else { + container "quay.io/biocontainers/nanoplot:1.32.1--py_0" + } + + input: + tuple val(meta), path(ontfile) + + output: + tuple val(meta), path("*.html"), emit: html + tuple val(meta), path("*.png") , emit: png + tuple val(meta), path("*.txt") , emit: txt + tuple val(meta), path("*.log") , emit: log + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def input_file = ("$ontfile".endsWith(".fastq.gz")) ? "--fastq ${ontfile}" : + ("$ontfile".endsWith(".txt")) ? "--summary ${ontfile}" : '' + """ + NanoPlot \\ + $options.args \\ + -t $task.cpus \\ + $input_file + echo \$(NanoPlot --version 2>&1) | sed 's/^.*NanoPlot //; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/software/nanoplot/meta.yml b/software/nanoplot/meta.yml new file mode 100644 index 00000000..4a63ba25 --- /dev/null +++ b/software/nanoplot/meta.yml @@ -0,0 +1,58 @@ +name: nanoplot +description: Run NanoPlot on nanopore-sequenced reads +keywords: + - quality control + - qc + - fastq + - sequencing summary + - nanopore +tools: + - nanoplot: + description: | + NanoPlot is a tool for ploting long-read sequencing data and + alignment. + homepage: http://nanoplot.bioinf.be + documentation: https://github.com/wdecoster/NanoPlot +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - fastq: + type: file + description: | + List of input basecalled-FastQ files. + - summary_txt: + type: file + description: | + List of sequenicng_summary.txt files from running basecalling. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: NanoPlot report + pattern: "*{.html}" + - png: + type: file + description: Plots generated by NanoPlot + pattern: "*{.png}" + - txt: + type: file + description: Stats from NanoPlot + pattern: "*{.txt}" + - log: + type: file + description: log file of NanoPlot run + pattern: "*{.log}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@yuukiiwa" diff --git a/tests/config/pytest_software.yml b/tests/config/pytest_software.yml index 5a6add6b..e154e08a 100644 --- a/tests/config/pytest_software.yml +++ b/tests/config/pytest_software.yml @@ -330,6 +330,10 @@ multiqc: - software/multiqc/** - tests/software/multiqc/** +nanoplot: + - software/nanoplot/** + - tests/software/nanoplot/** + optitype: - software/optitype/** - tests/software/optitype/** diff --git a/tests/config/test_data.config b/tests/config/test_data.config index 40f8387c..0c2eebad 100644 --- a/tests/config/test_data.config +++ b/tests/config/test_data.config @@ -67,6 +67,8 @@ params { test_sorted_bam_bai = "${test_data_dir}/genomics/sarscov2/nanopore/bam/test.sorted.bam.bai" test_fastq_gz = "${test_data_dir}/genomics/sarscov2/nanopore/fastq/test.fastq.gz" + + test_sequencing_summary = "${test_data_dir}/genomics/sarscov2/nanopore/sequencing_summary/test.sequencing_summary.txt" } } 'homo_sapiens' { diff --git a/tests/data/genomics/sarscov2/nanopore/sequencing_summary/test.sequencing_summary.txt b/tests/data/genomics/sarscov2/nanopore/sequencing_summary/test.sequencing_summary.txt new file mode 100644 index 00000000..396865dd --- /dev/null +++ b/tests/data/genomics/sarscov2/nanopore/sequencing_summary/test.sequencing_summary.txt @@ -0,0 +1,101 @@ +filename_fastq filename_fast5 read_id run_id channel mux start_time duration num_events passes_filtering template_start num_events_template template_duration sequence_length_template mean_qscore_template strand_score_template median_template mad_template pore_type experiment_id sample_id end_reason alias type barcode_arrangement barcode_full_arrangement barcode_kibarcode_variant barcode_score barcode_front_id barcode_front_score barcode_front_refseq barcode_front_foundseq barcode_front_foundseq_length barcode_front_begin_index barcode_rear_id barcode_rear_score barcode_rear_refseq barcode_rear_foundseq barcode_rear_foundseq_length barcode_rear_end_index +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0cc85c6c-ed86-422e-a093-d2b81089cc6b 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 306 4 26971.573750 1.528000 1222 TRUE 26971.921250 944 1.180500 365 9.618100 0.000000 72.982841 9.405005 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 95.449997 NB01_FWD 95.449997 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCAAACAACTTTCTTCAGCACC 46 21 NB01_REV 74.769234 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGGAAAAGTTGTCGGTGTCTTTGTGTTGGCCT 38 14 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0e51c070-d577-4293-8389-bd34fdda9eac 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 147 2 26989.666750 1.285750 1028 TRUE 26990.304250 518 0.648250 241 14.296299 0.000000 75.616249 9.028806 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 100.021370 NB01_FWD 94.400002 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAGACACCGACAACTTTCTTCAGCACC 44 22 NB01_REV 100.021370 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 40 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 031ff99b-b11f-40dc-9258-273ec97683f8 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 63 4 27032.160250 1.050000 840 TRUE 27032.554000 525 0.656250 256 10.158012 0.000000 73.547142 9.216906 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 94.974358 NB01_FWD 86.444450 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAGACACAAACAACTTTCTTCAGCACC 44 25 NB01_REV 94.974358 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACT 38 14 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 09b7f97c-fb32-4a4a-84d9-c45dfc2b46d0 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 129 2 27035.680000 0.918250 734 TRUE 27035.967500 504 0.630750 228 12.914272 0.000000 73.923347 8.088305 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 87.996292 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACGCAAAGACCTCCAAACAACTTTCTTCAGCACC 47 22 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1783d96c-3955-4405-b765-472c6286be46 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 59 1 27048.514500 1.462250 1169 TRUE 27049.225750 600 0.751000 287 12.799289 0.000000 73.735245 8.088305 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 18 NB01_REV 94.461540 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTCTAACCT 40 15 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 091fba78-028e-4ed2-a799-35e9ba64541c 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 484 3 27061.566000 1.278250 1022 TRUE 27062.144750 559 0.699500 267 13.482072 0.000000 71.666145 8.840705 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 23 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 17 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 048d5d3a-16ed-4f36-aeb9-3311dd9dc593 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 343 3 27068.331000 1.076500 861 TRUE 27068.513500 715 0.894000 359 11.253586 0.000000 66.587440 10.533607 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 91.237030 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGCGACAACCTCTTCAGCACC 46 21 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 10361e9b-159a-447d-8cf0-1976515f00f4 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 363 1 27104.404500 0.972000 777 TRUE 27104.450750 740 0.925750 374 13.731576 0.000000 72.606644 7.712105 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 99.760681 NB01_FWD 99.760681 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAAGACACCGACAACTTTCTTTCAGCACCT 40 22 NB01_REV 85.333336 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT TTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT 41 9 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 10508138-2f5e-4405-99f4-46af4452893c 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 506 3 27119.333250 0.926500 741 TRUE 27119.440750 655 0.819000 280 11.574930 0.000000 67.716042 10.909806 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 99.760681 NB01_FWD 99.760681 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAGAGACACCGACAACTTTCTTCAGCACCT 40 30 NB01_REV 94.533333 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAAGCTGTCGGTGTCTTTGTGTTAACCTTAGCAAT 45 11 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0bb9f17c-3b8f-486d-ac03-6550199a0a49 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 73 4 27214.258250 1.049750 839 TRUE 27214.515750 633 0.792250 261 12.703223 0.000000 73.170944 6.959704 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 20 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 08db75dc-49a9-459d-afc8-5ed1d8e47901 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 30 4 27240.282250 0.964000 771 TRUE 27240.484750 609 0.761500 305 13.149987 0.000000 69.032745 9.216906 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 91.733330 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGGCTAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 21 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 12 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 10f98781-e47e-43c3-9006-6d73963ee710 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 391 1 27273.886750 1.761250 1409 TRUE 27273.886750 1409 1.761250 295 11.646026 0.000000 69.032745 1.692901 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 24 NB01_REV 90.358978 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTTTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 41 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 01faaaa1-eca2-4bcd-8666-b4d69497107e 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 60 2 27285.488500 3.056000 2444 TRUE 27285.607250 2349 2.937250 962 13.226638 0.000000 67.716042 9.405005 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 99.760681 NB01_FWD 99.760681 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACCAAAGACACCGACAACTTTCTTCAGCACCT 40 26 NB01_REV 90.622223 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAAGTTTTCAGTGTCTTTGTGTTAACCTTAGCAAT 45 8 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 064ef037-67eb-49a4-9a72-e2b2e34cfe5c 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 392 2 27303.266750 1.275000 1020 TRUE 27303.315500 981 1.226250 261 8.430450 0.000000 85.773651 3.197702 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 94.533333 NB01_FWD 89.282051 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAGACACCGACAACTTTCTTCAGCACCT 37 7 NB01_REV 94.533333 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAAGTTGTCGGTTGTCTTGTGTTAACCTTAGCAAT 45 6 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 11e944d1-a2c9-409c-ac72-9c1563443df7 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 80 1 27337.413750 0.901500 721 TRUE 27337.513750 641 0.801500 319 11.575809 0.000000 72.418541 8.840705 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 89.384613 NB01_FWD 60.799999 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGAGAACTTGCCGACAACTTTCTTCAGCACC 50 23 NB01_REV 89.384613 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCAGTGTCTTGTGTTAACCT 38 12 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 022fc908-aa6e-4d29-a5ad-6a30a9da7e3b 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 17 4 27340.729500 1.401000 1120 TRUE 27341.374500 604 0.756000 251 13.240724 0.000000 71.289940 9.216906 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 91.866669 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTACTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 20 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0043238c-3a78-48f1-a8ec-d492280da6e4 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 166 3 27358.512250 0.908250 726 TRUE 27358.668500 601 0.752000 306 11.452598 0.000000 72.042343 7.335905 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 21 NB01_REV 61.250000 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGATGCTATAACAACT 46 7 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0a30e10c-26b5-4c23-a7ee-493e7b3ebffb 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 125 4 27361.921500 1.224250 979 TRUE 27362.424000 577 0.721750 296 11.683578 0.000000 77.873444 9.405005 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 16d9892d-bead-4a6f-b6c5-736c6f602fc1 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 399 1 27362.568750 1.114000 891 TRUE 27362.672500 808 1.010250 428 8.336836 0.000000 76.556747 7.900205 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 87.388885 NB01_FWD 80.051285 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAGATACGACAACTTTCTTCAGCACCT 36 30 NB01_REV 87.388885 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAAGAAAGTTGTCCAGTATCTTGTGTTAACCTTAGCAAT 46 4 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 00c6481f-9022-4173-9b74-f375c34b9a9b 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 267 1 27377.427750 1.569250 1255 TRUE 27377.656500 1072 1.340500 582 10.381586 0.000000 67.339844 8.840705 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 83.288887 NB01_FWD 83.288887 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTTTCTTCAGCACC 47 22 NB01_REV 65.897438 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAGTTTGTCGGTGTCTTTGCAACAACC 38 7 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1b86544f-8075-46a2-91f4-baac36216ac2 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 13 1 27403.046500 1.160500 928 TRUE 27403.407750 639 0.799250 270 11.101813 0.000000 79.566345 10.157406 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 92.858971 NB01_FWD 74.755554 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACCGAACACCGATGGCTTTCTTCAGCACC 44 21 NB01_REV 92.858971 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGCTAATCTTTGTGTTAACT 40 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 00068f7a-51b3-4933-8fc6-7d6e29181ff9 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 125 4 27436.449500 0.764250 611 TRUE 27436.525750 550 0.688000 291 10.191629 0.000000 79.002045 7.524004 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 98.307693 NB01_FWD 98.307693 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT 39 29 NB01_REV 94.622223 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT 44 8 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 084af0ac-0334-4840-a3a5-3ff62464ae71 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 266 2 27470.288000 0.675250 540 TRUE 27470.299250 531 0.664000 271 13.364845 0.000000 73.547142 9.028806 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1147d9c6-c190-47e6-8007-2e0f18d88f2b 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 484 3 27481.384250 0.594500 475 TRUE 27481.450500 422 0.528250 244 11.120881 0.000000 72.982841 9.781206 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 86.974358 NB01_FWD 67.511108 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAGGAACACCGACAACTTTCTTCTTCC 44 21 NB01_REV 86.974358 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGTGCTGAAGAAAGTTGTCAGTGTCTTTAATGTTAACCT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 004a65aa-6700-4ad7-9550-dc59f8708908 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 4 2 27482.850250 2.036250 1629 TRUE 27482.850250 1629 2.036250 231 11.287323 0.000000 64.894539 1.504801 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 24 NB01_REV 76.717949 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAGTTGTCAATGTCTTGTGTTAACT 36 15 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1721053e-98f4-47a1-ab93-f75bc1c03c79 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 486 2 27485.663250 0.960250 768 TRUE 27485.930750 554 0.692750 257 11.470281 0.000000 75.428146 8.276405 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 94.622223 NB01_FWD 80.564102 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTATACAAAGGCCACCGACAACTTTCTTCAGCACCT 39 29 NB01_REV 94.622223 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT 44 8 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 022647b9-0e16-4318-a794-c5749705b67d 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 327 3 27529.940250 0.985750 788 TRUE 27530.209000 573 0.717000 293 13.026576 0.000000 75.992447 9.028806 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 06f0339b-75b5-4926-b1a7-878818b5fe67 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 80 1 27560.648250 1.230250 984 TRUE 27561.094500 627 0.784000 277 10.914940 0.000000 72.606644 8.464505 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 91.866669 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAACACC 45 24 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 17 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 001ed67f-906f-4f37-9770-85d40f5ffee6 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 278 1 27583.466750 1.068750 855 TRUE 27583.671750 691 0.863750 271 17.272123 0.000000 74.487648 9.216906 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 23 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 123114b2-247c-4abc-be0c-3a94f02043a2 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 362 2 27589.458000 1.125500 900 TRUE 27589.504250 863 1.079250 446 9.773018 0.000000 75.804344 8.652605 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 67.377777 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAGGCACGACAACAACTAGCACC 40 25 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 18 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0af92aee-911c-4402-ba14-ecaabdb73548 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 26 1 27633.638750 1.131750 905 TRUE 27634.112500 526 0.658000 239 10.685639 0.000000 73.547142 8.840705 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 21 NB01_REV 94.461540 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCAT 40 14 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 04c07133-cd56-48b9-9f16-86e12cacaf92 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 360 2 27650.074500 1.400250 1120 TRUE 27650.538250 749 0.936500 365 13.548334 0.000000 71.854240 9.969306 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 21 NB01_REV 60.871796 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTAGCCAGTTAAT 40 12 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0b168ee0-74c4-494d-a593-a4b4b538a9cb 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 506 3 27718.938000 1.096750 877 TRUE 27719.354250 544 0.680500 272 12.718245 0.000000 74.111443 8.652605 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 20 NB01_REV 79.179489 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAGAGTTGTCGGTGTCTTTGTGGTAAC 37 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 18cef30b-9fe5-40b4-9094-0389bb5aeb8e 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 104 2 27725.970000 1.343000 1074 TRUE 27726.347500 772 0.965500 362 13.204041 0.000000 76.556747 7.712105 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 87.333336 NB01_FWD 87.333336 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGTTAACACAAAGGTACCGACAACTTTCTTCAGCACC 44 21 NB01_REV 71.641029 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCAGTGTCTTTGGCT 33 0 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 16325928-3440-4a49-94c5-a93b4f6da961 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 174 2 27749.025750 2.109500 1687 TRUE 27749.365750 1415 1.769500 605 10.853055 0.000000 67.716042 9.216906 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 100.985184 NB01_FWD 63.743587 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACACAAGGGCGACCAACTCTTCAACACCT 37 28 NB01_REV 100.985184 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT 47 6 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0a86654b-30ec-4271-9882-b490de473b5e 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 341 2 27851.303500 1.081250 865 TRUE 27851.743500 513 0.641250 278 9.593442 0.000000 75.240044 8.840705 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 88.177773 NB01_FWD 87.230774 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGATTCACAAAGACACCGACAACTTTCTTCAGCACCT 38 26 NB01_REV 88.177773 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAGTTGTCCGAATGTCTTTGTATTTAACCTTAGCAAT 47 11 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 018c4b52-48fa-4cc8-a7b4-500f41420279 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 117 1 27949.471500 0.991000 792 TRUE 27949.520250 753 0.942250 385 12.105667 0.000000 80.506851 8.652605 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 23 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0f3ca011-bb96-4fe2-92e2-31b0bac90d97 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 414 1 27960.445500 1.542000 1233 TRUE 27960.616750 1096 1.370750 507 15.022301 0.000000 82.764053 9.969306 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 94.400002 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACGAAGACACCGACAACTTTCTTCAGCACC 45 21 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1294c94b-1dac-4c4c-9c36-e8e8996e7f09 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 61 4 27999.728750 0.775250 620 TRUE 27999.792500 569 0.711500 303 9.327618 0.000000 70.725639 9.405005 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 86.000008 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAC 41 21 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 18 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 139c8fcc-8dcb-4cf7-aa52-632718587fe7 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 56 3 28001.593500 3.426000 2740 TRUE 28001.718500 2640 3.301000 1388 11.898949 0.000000 76.368645 9.781206 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 95.722221 NB01_FWD 93.794868 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAGACACCGACAACTTTCTTCAGCACCT 38 31 NB01_REV 95.722221 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAGTTGTCGGTGTCTTTAGTGTTTAACCTTAGCAAT 46 7 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 190c4417-c4cf-40fe-a0df-db4b495a3f77 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 433 2 28019.295750 0.971250 777 TRUE 28019.358250 727 0.908750 374 11.845625 0.000000 85.773651 6.583504 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 95.496300 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAGGAGCACCGACAACTTTCTTCAGCACC 46 23 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 15 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0c5e39bf-e1c5-4208-b110-9a00b55132e4 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 334 2 28024.842000 1.451750 1161 TRUE 28024.842000 1161 1.451750 282 13.246923 0.000000 74.675743 2.069101 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 23 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 18ee103f-1cf8-4694-b2e7-ac228fe70ccd 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 4 2 28032.892000 1.550000 1240 TRUE 28033.343250 879 1.098750 440 13.109956 0.000000 66.211243 7.712105 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 72.358971 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGTGCTGAAGTTGTCGGTGTCTTTGTATTTAACCT 35 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1a5c9192-aded-4e94-8dcb-9c04a402978e 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 379 3 28044.956000 1.297750 1038 TRUE 28045.576000 542 0.677750 276 12.664828 0.000000 75.616249 8.652605 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 99.760681 NB01_FWD 99.760681 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACCAAAGACACCGACAACTTTCTTCAGCACCT 40 31 NB01_REV 98.533333 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT 45 11 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1bd0dad7-1600-44a2-90b8-d5cd7b7caa6d 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 210 2 28059.863750 0.656750 525 TRUE 28059.893750 501 0.626750 275 9.448536 0.000000 74.487648 8.652605 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 21 NB01_REV 93.948715 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTGGTGTCTTTGTGTTAACCT 38 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1d568051-bdd6-4204-ac34-97b5a3f7375c 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 229 4 28075.092750 0.955750 764 TRUE 28075.139000 727 0.909500 362 11.821651 0.000000 75.804344 10.345507 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 95.059830 NB01_FWD 95.059830 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAGGAACACCGACAACTTTCTTCAGCACCT 40 23 NB01_REV 66.000000 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAGTTGTCAGTATCTTTGTTAACCTTATAT 40 7 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 064b4a28-a036-46cb-b466-62b8486de288 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 213 3 28091.644500 1.106250 885 TRUE 28091.984500 613 0.766250 252 13.016011 0.000000 72.230446 8.840705 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 94.755554 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTCAACACAAAGACACCGACAACTTTCTTCAGCACC 46 23 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 12 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 03e0e732-cb7a-4084-8fc9-303180c83786 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 232 4 28111.922750 2.026500 1621 TRUE 28112.147750 1441 1.801500 562 14.552203 0.000000 73.170944 9.969306 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 100.021370 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 21 NB01_REV 100.021370 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAAGTTGTCGGTGTCTTTGTGTTAACCT 40 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0e5018bc-45fc-40d4-8806-e22e07a915d0 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 8 4 28118.695750 2.406000 1924 TRUE 28118.695750 1924 2.406000 515 9.499933 0.000000 74.487648 2.445302 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 77.333328 NB01_FWD 77.333328 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTAACTAGGTTAACACAAAGACACTAACAACTTTCTTCAGCACC 45 22 NB01_REV 64.205124 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCAGTAATCTTTATTAGCACT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1a276f1c-5a5b-4ed2-b071-87f59f7c5233 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 164 1 28121.644000 0.804500 643 TRUE 28121.775250 538 0.673250 239 14.468572 0.000000 71.666145 8.464505 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 95.267090 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCAGTGTCTTTTGTGTTAACCT 40 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0cc2613c-9ade-4ec9-96c7-07ab2820bbd7 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 481 1 28129.377000 1.398750 1119 TRUE 28129.578250 958 1.197500 416 12.991942 0.000000 80.318748 9.405005 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 98.307693 NB01_FWD 98.307693 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT 39 31 NB01_REV 87.388885 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAAGTTATTAAGTGTCTTTGTGTTAACCTTAGCAAT 46 6 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 02085a68-dc6e-4275-bd1e-f112fed3bd83 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 454 3 28148.734750 0.703250 562 TRUE 28148.788500 519 0.649500 270 8.252804 0.000000 72.606644 8.276405 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 83.331192 NB01_FWD 60.177780 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTGGGGTTGGCACAAGGAGCACCGACAACTTTCTTCAGCACC 46 21 NB01_REV 83.331192 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGTGCTGAAGAAAGTTGTCGGTGTCTTGCTAATTAACT 38 15 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1377f442-7e38-4c3f-aacb-e8049ad884fe 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 376 1 28195.540000 1.479000 1183 TRUE 28195.751250 1014 1.267750 379 11.946314 0.000000 71.666145 10.909806 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 91.733330 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATGTGCTAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 20 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 11 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 069ce6e7-41b9-41b2-a6d8-8d39c16dbaa2 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 23 2 28203.369500 0.970750 776 TRUE 28203.469500 696 0.870750 238 12.972846 0.000000 55.113335 7.335905 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 94.622223 NB01_FWD 78.410255 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGTTAACACAAAGACACCGACAACTCTTCAACACCT 36 30 NB01_REV 94.622223 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT 44 11 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 05f48799-d4ca-4f54-90e2-de208c0c1711 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 61 4 28207.386000 1.197000 957 TRUE 28207.391000 953 1.192000 344 11.156427 0.000000 74.299545 7.524004 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 99.760681 NB01_FWD 99.760681 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAAGAACACCGACAACTTTCTTCAGCACCT 40 30 NB01_REV 88.533333 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAACTAAT 46 1 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 2166cca5-c0ab-49eb-aec1-fec09bdfbd83 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 470 4 28253.256750 1.155000 924 TRUE 28253.319250 874 1.092500 426 12.736054 0.000000 66.775543 7.900205 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 90.410255 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCAGTGTCTTTGTGTTAACT 38 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0dad8e5f-77e3-4fd1-b569-b06f5b87d8c3 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 428 1 28261.361500 4.703500 3762 TRUE 28261.596500 3574 4.468500 1664 14.312189 0.000000 76.180550 9.593106 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 87.333336 NB01_FWD 82.533333 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCATTTCTTTCTTCAGCACC 44 21 NB01_REV 87.333336 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTCAGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 38 17 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0457184e-6e09-4883-baf3-824d11101c43 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 350 1 28283.065000 1.576750 1261 TRUE 28283.236250 1124 1.405500 493 14.191508 0.000000 69.408943 10.533607 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 75.384621 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAGAAAGTTGTCGGTGTCTTTGTGTGTAGC 37 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1d650a9e-4e67-456a-9abe-bd34b238abe4 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 315 2 28293.463750 0.919000 735 TRUE 28293.688750 555 0.694000 288 9.755042 0.000000 72.982841 11.097907 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 90.488892 NB01_FWD 90.488892 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACCCTTTCTTCAGCACC 44 22 NB01_REV 80.820511 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTT 34 0 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0565fd27-4849-4d14-83ff-2864dc3e109e 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 341 1 28312.481250 0.983250 786 TRUE 28312.545000 735 0.919500 352 11.441270 0.000000 72.982841 10.533607 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 83.022217 NB01_FWD 83.022217 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTTAACACGAACACCGACAACTTTCTTCAGCACC 44 22 NB01_REV 63.743591 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT GGTTGCTTGAGAGGATTATCGTGTCTTTGTGTTAACCT 38 15 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 190a9ebe-4d5f-43c0-a495-82ff58944d07 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 8 4 28317.539500 0.831250 665 TRUE 28317.589500 625 0.781250 277 13.174835 0.000000 72.982841 8.840705 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 99.796295 NB01_FWD 94.769234 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT 38 29 NB01_REV 99.796295 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT 46 12 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 04a0ee1e-0406-4394-87ba-a67344318a5d 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 431 4 28334.032750 1.612250 1289 TRUE 28334.445250 959 1.199750 440 11.334149 0.000000 69.973244 9.405005 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 20 NB01_REV 69.418800 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGAAATTGTCTTTATGTGTTAACCT 44 17 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 12ac7997-50bc-4183-95c4-e953ad4eaad1 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 172 3 28422.977000 2.067500 1654 TRUE 28423.174500 1496 1.870000 523 13.648002 0.000000 73.547142 9.028806 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 26 NB01_REV 84.820511 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTATTAACCT 37 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 13dbd26f-978f-49ad-9ae6-ca909083b6ce 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 414 1 28428.023250 0.967500 774 TRUE 28428.290750 560 0.700000 269 14.325933 0.000000 82.199753 10.345507 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 90.871796 NB01_FWD 90.871796 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAAGACACCGACAACTTTCTTCAACACCT 39 29 NB01_REV 86.622223 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAAGTTGTCAGTCTTTGTGTTAACCTTAGCAAT 43 9 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 13265c3e-bbb3-4ebe-b4db-9fbb4bdaff01 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 227 4 28429.552250 1.043750 835 TRUE 28429.731000 692 0.865000 253 13.674288 0.000000 75.051949 8.464505 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 24 NB01_REV 83.794876 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTATAC 37 12 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 188b5eae-fce2-439c-b490-aff6c4327499 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 85 4 28431.610500 0.911000 728 TRUE 28431.693000 662 0.828500 275 14.811317 0.000000 74.863846 9.028806 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 94.577782 NB01_FWD 93.794876 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAAGGCACCGACAACTTTCTTCAGCACCT 39 29 NB01_REV 94.577782 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAAGTTGTCAGTGTCTTTGTGTTAACCTTAGCAAT 45 9 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1730fabf-f1ac-4a02-aa2e-21dcd14fed00 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 99 4 28430.941500 2.444750 1955 TRUE 28431.100250 1828 2.286000 662 16.376518 0.000000 74.487648 9.028806 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 94.755554 NB01_FWD 94.755554 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGAGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 46 17 NB01_REV 66.102562 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAAGTTGTCGGTGTGTTTGTGTTA 32 0 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 151bd13c-c12f-41a6-be02-e97e70a57814 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 258 3 28435.662750 1.241250 993 TRUE 28435.965250 751 0.938750 301 12.750878 0.000000 68.280342 9.405005 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 99.760681 NB01_FWD 99.760681 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAAAGACACCGACAACTTTCTTCAGCACCT 40 32 NB01_REV 79.155556 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTGCTCCAGCAAT 43 11 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1a0c9927-e355-49c3-877a-e6b95bc1a402 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 370 4 28473.545500 1.141500 913 TRUE 28473.584250 882 1.102750 377 12.813323 0.000000 72.982841 7.524004 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 86.577782 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAGGAGCACCGACCTTTCTTCAGCACC 44 22 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 17 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0d448640-aa67-4e58-9ce8-004ca23b0412 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 330 2 28476.688500 1.505750 1204 TRUE 28477.348500 676 0.845750 324 11.969595 0.000000 71.478043 9.969306 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 101.393158 NB01_FWD 88.292587 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGAAGACACCGACAACTTTCTTCAGCACC 48 22 NB01_REV 101.393158 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 41 15 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 20586d1a-2cd0-45aa-abc2-04ac66d8ea46 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 247 2 28512.948000 1.407500 1126 TRUE 28512.948000 1126 1.407500 268 10.520182 0.000000 67.716042 2.257201 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 94.711113 NB01_FWD 94.711113 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTCTTACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 94.051285 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCT 38 11 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0b8b3929-730d-42a6-84e0-5689b7439c71 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 283 2 28529.018750 1.439000 1151 TRUE 28529.383750 859 1.074000 424 11.602247 0.000000 73.547142 9.593106 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 93.794876 NB01_FWD 93.794876 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAAGACGCCGACAACTTTCTTCAGCACCT 39 29 NB01_REV 69.555557 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT TTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACAACAAT 38 7 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0e5ff4de-814b-4ee2-bf8f-3291b8bbdaae 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 47 2 28572.512750 1.723000 1378 TRUE 28573.329000 725 0.906750 318 12.203794 0.000000 65.270737 7.524004 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 99.760681 NB01_FWD 99.760681 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACCAAAGACACCGACAACTTTCTTCAGCACCT 40 30 NB01_REV 86.755554 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAAGTTGTCGGTGTATCTTTGTGTTAACCTTAGCAAT 44 10 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1b760e8d-7b17-47e0-ba96-5755fc4ddd63 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 369 4 28576.069250 1.845000 1476 TRUE 28576.206750 1366 1.707500 493 14.775167 0.000000 75.992447 11.097907 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 90.512817 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAATTTGCCAGGTGTCTTTGTGTTAACCT 40 15 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1870a88c-439f-4125-a06c-38b1bf4416e4 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 248 2 28625.244750 0.985250 788 TRUE 28625.352250 702 0.877750 344 11.446499 0.000000 76.180550 8.652605 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 27 NB01_REV 90.871796 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACAT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 052036a3-6af0-4650-9f96-e97e34c52bbc 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 17 4 28639.270250 1.212500 970 TRUE 28639.516500 773 0.966250 300 13.807242 0.000000 76.932945 8.464505 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 24 NB01_REV 97.602562 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAAGAAGGTTACGTCGGTGTCTTTGTGTTAACCT 42 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0f7db51f-ff49-45ea-b58a-9fb58daf249d 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 117 1 28640.949750 1.228500 982 TRUE 28641.084750 874 1.093500 425 11.355693 0.000000 76.180550 10.157406 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 17 NB01_REV 93.948715 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTGTGTTAACCT 38 11 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 2109d790-67ec-4fd1-8931-6c7e61908ff3 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 243 1 28651.981500 1.105500 884 TRUE 28652.312750 619 0.774250 290 11.487652 0.000000 72.042343 8.652605 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 81.179489 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGAGTGCTGAAGAAAGTTGTCAGTGTCTTGTGTTAACCCT 40 18 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1af84d7e-8fc6-4dba-8750-a0d701f36d1d 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 414 1 28654.360750 0.823000 658 TRUE 28654.450750 586 0.733000 266 14.223591 0.000000 80.883049 9.216906 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 90.488884 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACCTGAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0fd3ec80-6fb4-4378-bdb6-df4f84951049 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 339 1 28655.925750 1.127250 901 TRUE 28656.123250 743 0.929750 354 11.615380 0.000000 81.823547 6.019204 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 86.358978 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACAT 38 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 137cae6b-0009-4ee2-8a0e-7a2e88179c16 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 504 2 28663.982750 1.030250 824 TRUE 28664.026500 789 0.986500 435 10.479577 0.000000 71.478043 8.840705 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 74.344444 NB01_FWD 74.344444 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACCACAAAGACTTCAAACAACTTTCTTCAACACC 49 21 NB01_REV 71.435898 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAGTTGTCAGTGTCTTTTGTGTGCT 36 15 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 045f1e6a-9b5d-4840-bad9-c4b738b61267 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 1 2 28703.039000 1.466250 1173 TRUE 28703.500250 804 1.005000 344 12.363604 0.000000 71.101845 9.216906 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 90.844452 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC TGTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 20 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 066c72de-139a-4514-aef9-36cc3927a2c6 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 12 4 28721.898250 0.862750 690 TRUE 28722.119500 513 0.641500 275 7.734092 0.000000 80.694946 8.276405 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 94.400002 NB01_FWD 94.400002 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAGCTTTCTTCAGCACC 45 23 NB01_REV 67.538460 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCAGTGTCTGTGTGCAT 35 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 00da3999-12fd-449d-bd07-32a879667a3d 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 125 4 28722.596250 1.644500 1315 TRUE 28722.596250 1315 1.644500 272 11.603066 0.000000 75.240044 1.881001 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 86.577782 NB01_FWD 86.577782 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCAGCTGCTTTCTTCAGCACC 45 20 NB01_REV 72.461540 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTGTTAACAT 35 11 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 094a4c34-c97b-4b83-bd02-1f69e6ccadc0 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 418 1 28726.230500 1.353250 1082 TRUE 28726.739250 675 0.844500 256 13.964858 0.000000 70.349442 8.464505 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 94.000000 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTATTAACCT 39 15 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 180da5c9-4574-48ab-8e6a-8f8a73767e30 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 49 2 28782.142000 1.068500 854 TRUE 28782.478250 585 0.732250 280 13.520915 0.000000 70.725639 9.405005 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 20 NB01_REV 84.974358 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAAGTTGTCGGTGTCTTTGTGTTAACCT 36 13 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 206626c5-ccf2-4927-9987-164f627bdede 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 276 3 28789.548750 1.058750 847 TRUE 28790.027500 464 0.580000 241 10.530326 0.000000 71.666145 10.533607 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 85.025642 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGGGATTGTCGGTGTCTTTGTGTTAACCT 38 17 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 13bc3b77-a88e-44b9-b119-cd56aa972f61 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 169 2 28801.996250 1.068000 854 TRUE 28802.275000 631 0.789250 296 11.052993 0.000000 73.923347 9.216906 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 94.400002 NB01_FWD 94.400002 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAGCTTTCTTCAGCACC 45 17 NB01_REV 82.273499 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGTGCTGAAAGAAGTTGTCAATTGTCTTTGTGTTAACCT 39 17 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 046258fa-b951-43a1-bbed-66cdbeac8e71 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 233 2 28810.953000 1.217250 973 TRUE 28811.376750 634 0.793500 300 12.088890 0.000000 76.932945 8.840705 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 84.974358 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAAGTTGTCGGTGTCTTTGTGTTAACCT 36 14 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1d86c2b3-c2c6-43a1-97ed-8c1f0cbf6476 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 26 3 28835.020500 3.819250 3055 TRUE 28835.360500 2783 3.479250 1180 13.237278 0.000000 73.359047 8.276405 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 22 NB01_REV 88.675217 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT GGTGCTGAGAAGAAAGTTGTCGGTGTCTTTGTGTGTTAACCT 42 15 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 08239d1b-1710-4187-b96e-da861a0e4624 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 55 1 28844.006250 0.946000 756 TRUE 28844.063750 710 0.888500 347 11.407701 0.000000 71.289940 8.276405 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 19 NB01_REV 80.256409 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCAGTGTCTTTATTAACCT 37 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 11e4024c-1997-4372-b1d7-4c884cf58047 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 418 1 28864.370250 1.403500 1122 TRUE 28864.477750 1036 1.296000 477 9.518910 0.000000 68.844643 9.969306 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 84.977776 NB01_FWD 84.977776 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAAGTTAACACGAGGAGCACCGACAACTTTCTTCAGCACC 46 21 NB01_REV 71.384613 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAATTGTCAGTGTCTTTGTGTGCT 36 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 074ebef4-edbd-4d2a-af6c-46512693a990 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 43 3 28882.090250 1.219250 975 TRUE 28882.160250 919 1.149250 415 11.225968 0.000000 67.716042 9.405005 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 95.496300 NB01_FWD 95.496300 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGCACCGACAACTTGCTCTTCAGCACC 46 23 NB01_REV 75.230766 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTCT 33 0 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 02445dc2-39ee-49f1-8a42-51ebfea52c33 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 508 1 28923.037750 2.300750 1840 TRUE 28923.519000 1455 1.819500 599 13.009890 0.000000 72.794746 9.028806 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 98.307693 NB01_FWD 98.307693 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT 39 28 NB01_REV 64.427780 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAAGAAGTTGTCAGTGTCTTTATTATTATTGTAAT 42 10 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 1b753736-87b1-4753-ab1d-bfd7e2d94e9e 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 28 1 28934.584250 1.655750 1324 TRUE 28934.648000 1273 1.592000 582 14.511514 0.000000 70.913742 9.028806 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.311111 NB01_FWD 98.311111 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC 45 23 NB01_REV 94.051285 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAGTTGTCGGTGTCTTTGTGTTAACCT 38 16 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0562aebd-b55b-4752-83eb-12323938c242 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 442 3 28969.634250 1.884750 1507 TRUE 28970.029250 1191 1.489750 619 9.930101 0.000000 73.923347 9.405005 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 98.307693 NB01_FWD 98.307693 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT 39 28 NB01_REV 70.711113 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GGTGCTGAAGAAAGTTGTCAGGTATCTTTGTGTATAGCAAT 41 6 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0f06cd06-1136-4b10-b248-cd4324f6de22 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 6 1 28982.300250 1.049250 839 TRUE 28982.465250 707 0.884250 331 13.276942 0.000000 73.170944 9.969306 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var1 NB var1 75.538460 NB01_FWD 75.538460 AGGTTAACACAAAGACACCGACAACTTTCTTCAGCACCT AGGTTAACACCGAGGCATGACTAACTTTCTTCAGCACCT 39 29 NB01_REV 73.855560 GGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCTTAGCAAT GATCACTGAAGAAAGTTGTCGGTGTCTTTTTATGTTAGCCTTAGCAAT 48 5 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0a92fbf4-99b4-40cd-98e7-93cfbddeb35e 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 90 2 28993.728000 1.485000 1188 TRUE 28994.364250 679 0.848750 323 13.160778 0.000000 74.299545 9.969306 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 94.400002 NB01_FWD 94.400002 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATTGCTAAGGTTAACACAAAGACACCGACAGCTTTCTTCAGCACC 45 22 NB01_REV 69.435890 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAAGTTGTCCCGGTGTCTTTTGGTAGCCT 37 18 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 0dd4696c-a7dd-4e3a-9ce5-37a258b45373 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 157 1 29000.028500 1.448500 1158 TRUE 29000.028500 1158 1.448500 244 9.649623 0.000000 66.399338 1.692901 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 98.564102 NB01_FWD 83.644447 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATGCATAAGGTTAACACAAAAGACACCGACAACTCTTCAGCACC 44 28 NB01_REV 98.564102 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT 39 14 +FAP51364_pass_barcode01_97ca62ca_11.fastq FAP51364_pass_barcode01_97ca62ca_11.fast5 10ac42d4-2818-48f6-9d67-1c3019fde890 97ca62ca093ff43533aa34c38a10b1d6325e7e7b 367 1 29006.491500 1.150500 920 TRUE 29006.754000 710 0.888000 329 10.715900 0.000000 73.547142 10.721706 not_set data RN20097 signal_positive barcode01 test_sample barcode01 NB01_var2 NB var2 94.000000 NB01_FWD 62.888889 ATTGCTAAGGTTAACACAAAGACACCGACAACTTTCTTCAGCACC ATCTGAAGGTTGCTAGACACCGACAGCTTTCTTCAGCACC 40 26 NB01_REV 94.000000 AGGTGCTGAAGAAAGTTGTCGGTGTCTTTGTGTTAACCT AGGTGCTGAAGAAAGTTGTCGGTGTCTTTATGTTAACCT 39 16 diff --git a/tests/software/nanoplot/main.nf b/tests/software/nanoplot/main.nf new file mode 100644 index 00000000..0000cb31 --- /dev/null +++ b/tests/software/nanoplot/main.nf @@ -0,0 +1,21 @@ +#!/usr/bin/env nextflow + +nextflow.enable.dsl = 2 + +include { NANOPLOT } from '../../../software/nanoplot/main.nf' addParams( options: [:] ) + +workflow test_nanoplot_summary { + def input = [] + input = [ [ id:'test' ], // meta map + [ file(params.test_data['sarscov2']['nanopore']['test_sequencing_summary'], checkIfExists: true) ] ] + + NANOPLOT ( input ) +} + +workflow test_nanoplot_fastq { + def input = [] + input = [ [ id:'test' ], // meta map + [ file(params.test_data['sarscov2']['nanopore']['test_fastq_gz'], checkIfExists: true) ] ] + + NANOPLOT ( input ) +} diff --git a/tests/software/nanoplot/test.yml b/tests/software/nanoplot/test.yml new file mode 100644 index 00000000..0ad55a5a --- /dev/null +++ b/tests/software/nanoplot/test.yml @@ -0,0 +1,17 @@ + +- name: nanoplot_summary + command: nextflow run ./tests/software/nanoplot -entry test_nanoplot_summary -c tests/config/nextflow.config + tags: + - nanoplot + files: + - path: ./output/nanoplot/NanoPlot-report.html + contains: + - "report" +- name: nanoplot_fastq + command: nextflow run ./tests/software/nanoplot -entry test_nanoplot_fastq -c tests/config/nextflow.config + tags: + - nanoplot + files: + - path: ./output/nanoplot/NanoPlot-report.html + contains: + - "report"