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Added complement module with necessary test dir/parameters
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59
software/bedtools/complement/functions.nf
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59
software/bedtools/complement/functions.nf
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/*
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* -----------------------------------------------------
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* Utility functions used in nf-core DSL2 module files
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* -----------------------------------------------------
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*/
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/*
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* Extract name of software tool from process name using $task.process
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*/
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def getSoftwareName(task_process) {
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return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
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}
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/*
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* Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
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*/
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def initOptions(Map args) {
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def Map options = [:]
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options.args = args.args ?: ''
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options.args2 = args.args2 ?: ''
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options.publish_by_id = args.publish_by_id ?: false
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options.publish_dir = args.publish_dir ?: ''
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options.publish_files = args.publish_files
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options.suffix = args.suffix ?: ''
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return options
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}
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/*
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* Tidy up and join elements of a list to return a path string
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*/
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def getPathFromList(path_list) {
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def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries
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paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
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return paths.join('/')
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}
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/*
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* Function to save/publish module results
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*/
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def saveFiles(Map args) {
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if (!args.filename.endsWith('.version.txt')) {
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def ioptions = initOptions(args.options)
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def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
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if (ioptions.publish_by_id) {
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path_list.add(args.publish_id)
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}
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if (ioptions.publish_files instanceof Map) {
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for (ext in ioptions.publish_files) {
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if (args.filename.endsWith(ext.key)) {
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def ext_list = path_list.collect()
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ext_list.add(ext.value)
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return "${getPathFromList(ext_list)}/$args.filename"
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}
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}
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} else if (ioptions.publish_files == null) {
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return "${getPathFromList(path_list)}/$args.filename"
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}
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}
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}
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36
software/bedtools/complement/main.nf
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36
software/bedtools/complement/main.nf
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// Import generic module functions
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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def options = initOptions(params.options)
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process BEDTOOLS_COMPLEMENT {
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tag "$meta.id"
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label 'process_medium'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
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conda (params.enable_conda ? "bioconda::bedtools =2.29.2" : null)
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if (workflow.containerEngine == 'singularity' && !params.pull_docker_container) {
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container "https://depot.galaxyproject.org/singularity/bedtools:2.29.2--hc088bd4_0 "
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} else {
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container "quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0"
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}
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input:
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tuple val(meta), path(beds)
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path sizes
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output:
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tuple val(meta), path("*.complement.bed"), emit: complement
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path "*.version.txt", emit: version
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script:
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def software = getSoftwareName(task.process)
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def beds_files = beds.sort()
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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"""
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bedtools complement -i ${beds[0]} -g $sizes ${options.args} > ${prefix}.complement.bed
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bedtools --version | sed -e "s/Bedtools v//g" > ${software}.version.txt
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"""
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}
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54
software/bedtools/complement/meta.yml
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54
software/bedtools/complement/meta.yml
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name: bedtools_complement
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description: Returns all intervals in a genome that are not covered by at least one interval in the input BED/GFF/VCF file.
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keywords:
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- bed
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- complement
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tools:
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- bedtools:
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description: |
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A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types.
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documentation: https://bedtools.readthedocs.io/en/latest/
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params:
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- outdir:
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type: string
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description: |
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The pipeline's output directory. By default, the module will
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output files into `$params.outdir/<SOFTWARE>`
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- publish_dir_mode:
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type: string
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description: |
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Value for the Nextflow `publishDir` mode parameter.
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Available: symlink, rellink, link, copy, copyNoFollow, move.
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- enable_conda:
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type: boolean
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description: |
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Run the module with Conda using the software specified
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via the `conda` directive
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input:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- bed:
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type: file
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description: List of bed files
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pattern: "*.{bed}"
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output:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- bed:
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type: file
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description: Edited bed file
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pattern: "*.{slopbed}"
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- version:
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type: file
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description: File containing software version
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pattern: "*.{version.txt}"
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5
software/bedtools/complement/test/input/A.bed
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5
software/bedtools/complement/test/input/A.bed
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chr1 951 1061
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chr1 1300 1420
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chr1 1400 1500
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1
software/bedtools/complement/test/input/genome.sizes
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1
software/bedtools/complement/test/input/genome.sizes
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chr1 1780869
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24
software/bedtools/complement/test/main.nf
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24
software/bedtools/complement/test/main.nf
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#!/usr/bin/env nextflow
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nextflow.preview.dsl = 2
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include { BEDTOOLS_COMPLEMENT } from '../main.nf' addParams( options: [publish_dir:'test_bed_file'] )
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// Define input channels
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// Run the workflow
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workflow test_bed_file {
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def input = []
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input = [ [ id:'test'],
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[ file("${baseDir}/input/A.bed", checkIfExists: true),] ]
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BEDTOOLS_COMPLEMENT(
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input,
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file("${baseDir}/input/genome.sizes", checkIfExists: true)
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)
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}
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workflow {
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test_bed_file()
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}
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20
software/bedtools/complement/test/nextflow.config
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20
software/bedtools/complement/test/nextflow.config
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params {
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outdir = "output/"
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publish_dir_mode = "copy"
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enable_conda = false
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}
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profiles {
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conda {
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params.enable_conda = true
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}
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docker {
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docker.enabled = true
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docker.runOptions = '-u \$(id -u):\$(id -g)'
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}
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singularity {
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singularity.enabled = true
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singularity.autoMounts = true
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}
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}
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chr1 0 951
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chr1 1061 1300
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chr1 1500 1780869
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