Added complement module with necessary test dir/parameters

This commit is contained in:
Sruthi Suresh 2020-11-11 17:02:40 -06:00 committed by sruthipsuresh
parent 709970e9af
commit 199dfc5ea3
8 changed files with 202 additions and 0 deletions

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/*
* -----------------------------------------------------
* Utility functions used in nf-core DSL2 module files
* -----------------------------------------------------
*/
/*
* Extract name of software tool from process name using $task.process
*/
def getSoftwareName(task_process) {
return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
}
/*
* Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
*/
def initOptions(Map args) {
def Map options = [:]
options.args = args.args ?: ''
options.args2 = args.args2 ?: ''
options.publish_by_id = args.publish_by_id ?: false
options.publish_dir = args.publish_dir ?: ''
options.publish_files = args.publish_files
options.suffix = args.suffix ?: ''
return options
}
/*
* Tidy up and join elements of a list to return a path string
*/
def getPathFromList(path_list) {
def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries
paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
return paths.join('/')
}
/*
* Function to save/publish module results
*/
def saveFiles(Map args) {
if (!args.filename.endsWith('.version.txt')) {
def ioptions = initOptions(args.options)
def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
if (ioptions.publish_by_id) {
path_list.add(args.publish_id)
}
if (ioptions.publish_files instanceof Map) {
for (ext in ioptions.publish_files) {
if (args.filename.endsWith(ext.key)) {
def ext_list = path_list.collect()
ext_list.add(ext.value)
return "${getPathFromList(ext_list)}/$args.filename"
}
}
} else if (ioptions.publish_files == null) {
return "${getPathFromList(path_list)}/$args.filename"
}
}
}

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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
def options = initOptions(params.options)
process BEDTOOLS_COMPLEMENT {
tag "$meta.id"
label 'process_medium'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
conda (params.enable_conda ? "bioconda::bedtools =2.29.2" : null)
if (workflow.containerEngine == 'singularity' && !params.pull_docker_container) {
container "https://depot.galaxyproject.org/singularity/bedtools:2.29.2--hc088bd4_0 "
} else {
container "quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0"
}
input:
tuple val(meta), path(beds)
path sizes
output:
tuple val(meta), path("*.complement.bed"), emit: complement
path "*.version.txt", emit: version
script:
def software = getSoftwareName(task.process)
def beds_files = beds.sort()
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
"""
bedtools complement -i ${beds[0]} -g $sizes ${options.args} > ${prefix}.complement.bed
bedtools --version | sed -e "s/Bedtools v//g" > ${software}.version.txt
"""
}

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name: bedtools_complement
description: Returns all intervals in a genome that are not covered by at least one interval in the input BED/GFF/VCF file.
keywords:
- bed
- complement
tools:
- bedtools:
description: |
A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types.
documentation: https://bedtools.readthedocs.io/en/latest/
params:
- outdir:
type: string
description: |
The pipeline's output directory. By default, the module will
output files into `$params.outdir/<SOFTWARE>`
- publish_dir_mode:
type: string
description: |
Value for the Nextflow `publishDir` mode parameter.
Available: symlink, rellink, link, copy, copyNoFollow, move.
- enable_conda:
type: boolean
description: |
Run the module with Conda using the software specified
via the `conda` directive
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- bed:
type: file
description: List of bed files
pattern: "*.{bed}"
output:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- bed:
type: file
description: Edited bed file
pattern: "*.{slopbed}"
- version:
type: file
description: File containing software version
pattern: "*.{version.txt}"

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chr1 951 1061
chr1 1300 1420
chr1 1400 1500

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chr1 1780869

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#!/usr/bin/env nextflow
nextflow.preview.dsl = 2
include { BEDTOOLS_COMPLEMENT } from '../main.nf' addParams( options: [publish_dir:'test_bed_file'] )
// Define input channels
// Run the workflow
workflow test_bed_file {
def input = []
input = [ [ id:'test'],
[ file("${baseDir}/input/A.bed", checkIfExists: true),] ]
BEDTOOLS_COMPLEMENT(
input,
file("${baseDir}/input/genome.sizes", checkIfExists: true)
)
}
workflow {
test_bed_file()
}

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params {
outdir = "output/"
publish_dir_mode = "copy"
enable_conda = false
}
profiles {
conda {
params.enable_conda = true
}
docker {
docker.enabled = true
docker.runOptions = '-u \$(id -u):\$(id -g)'
}
singularity {
singularity.enabled = true
singularity.autoMounts = true
}
}

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chr1 0 951
chr1 1061 1300
chr1 1500 1780869