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Update TIDDIT to 3.0.0
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2 changed files with 29 additions and 11 deletions
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@ -2,15 +2,16 @@ process TIDDIT_SV {
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tag "$meta.id"
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label 'process_medium'
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conda (params.enable_conda ? "bioconda::tiddit=2.12.1" : null)
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conda (params.enable_conda ? "bioconda::tiddit=3.0.0" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/tiddit:2.12.1--py38h1773678_0' :
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'quay.io/biocontainers/tiddit:2.12.1--py38h1773678_0' }"
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'https://depot.galaxyproject.org/singularity/tiddit:3.0.0--py39h59fae87_1' :
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'quay.io/biocontainers/tiddit:3.0.0--py39h59fae87_1' }"
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input:
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tuple val(meta), path(bam)
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tuple val(meta), path(input)
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path fasta
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path fai
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path bwa_index
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output:
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tuple val(meta), path("*.vcf") , emit: vcf
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@ -26,10 +27,12 @@ process TIDDIT_SV {
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def prefix = task.ext.prefix ?: "${meta.id}"
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def reference = fasta ? "--ref $fasta" : ""
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"""
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INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'`
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tiddit \\
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--sv \\
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$args \\
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--bam $bam \\
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--bam $input \\
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$reference \\
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-o $prefix
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@ -2,25 +2,40 @@
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nextflow.enable.dsl = 2
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include { BWA_INDEX } from '../../../../modules/bwa/index/main.nf'
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include { TIDDIT_SV } from '../../../../modules/tiddit/sv/main.nf'
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workflow test_tiddit_sv {
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input = [
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input = [
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[ id:'test' ], // meta map
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[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
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[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
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]
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fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
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fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
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TIDDIT_SV ( input, fasta, fai )
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BWA_INDEX( fasta )
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TIDDIT_SV ( input, fasta, fai , BWA_INDEX.out.index)
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}
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workflow test_tiddit_sv_no_ref {
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input = [
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input = [
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[ id:'test' ], // meta map
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[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
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[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
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]
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TIDDIT_SV ( input, [], [] )
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}
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workflow test_tiddit_sv_cram {
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input = [
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[ id:'test' ], // meta map
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[ file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true) ]
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]
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fasta = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
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fai = file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true)
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TIDDIT_SV ( input, fasta, fai )
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}
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