Add MEGAN/RMA2INFO (#1513)

* fix: remove left-over unnecessary code

* Add megan/rma2info

* Attempt at fixing test

* Right yml formatting

* Get the versios reporting correct
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James A. Fellows Yates 2022-04-12 08:35:36 +02:00 committed by GitHub
parent 897c33d5da
commit 2d38566eca
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6 changed files with 130 additions and 0 deletions

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process MEGAN_RMA2INFO {
tag "$meta.id"
label 'process_low'
conda (params.enable_conda ? "bioconda::megan=6.21.7" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/megan:6.21.7--h9ee0642_0':
'quay.io/biocontainers/megan:6.21.7--h9ee0642_0' }"
input:
tuple val(meta), path(rma6)
val(megan_summary)
output:
tuple val(meta), path("*.txt.gz") , emit: txt
tuple val(meta), path("*.megan"), optional: true, emit: megan_summary
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def summary = megan_summary ? "-es ${prefix}.megan" : ""
"""
rma2info \\
-i ${rma6} \\
-o ${prefix}.txt.gz \\
${summary} \\
$args
cat <<-END_VERSIONS > versions.yml
"${task.process}":
megan: \$(echo \$(rma2info 2>&1) | grep version | sed 's/.*version //g;s/, built.*//g')
END_VERSIONS
"""
}

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name: "megan_rma2info"
description: Analyses an RMA file and exports information in text format
keywords:
- megan
- rma6
- classification
- conversion
tools:
- "megan":
description: "A tool for studying the taxonomic content of a set of DNA reads"
homepage: "https://uni-tuebingen.de/fakultaeten/mathematisch-naturwissenschaftliche-fakultaet/fachbereiche/informatik/lehrstuehle/algorithms-in-bioinformatics/software/megan6/"
documentation: "https://software-ab.informatik.uni-tuebingen.de/download/megan6/welcome.html"
tool_dev_url: "https://github.com/husonlab/megan-ce"
doi: "10.1371/journal.pcbi.1004957"
licence: "['GPL >=3']"
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- rma6:
type: file
description: RMA6 file from MEGAN or MALT
pattern: "*.rma6"
- megan_summary:
type: boolean
description: Specify whether to generate an MEGAN summary file
output:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
- txt:
type: file
description: Compressed text file
pattern: "*.txt.gz"
- megan_summary:
type: file
description: Optionally generated MEGAN summary file
pattern: "*.megan"
authors:
- "@jfy133"

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@ -1138,6 +1138,10 @@ megahit:
- modules/megahit/** - modules/megahit/**
- tests/modules/megahit/** - tests/modules/megahit/**
megan/rma2info:
- modules/megan/rma2info/**
- tests/modules/megan/rma2info/**
meningotype: meningotype:
- modules/meningotype/** - modules/meningotype/**
- tests/modules/meningotype/** - tests/modules/meningotype/**

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@ -0,0 +1,16 @@
#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
include { MEGAN_RMA2INFO } from '../../../../modules/megan/rma2info/main.nf'
workflow test_megan_rma2info {
input = [
[ id:'test', single_end:false ], // meta map
file('https://github.com/nf-core/test-datasets/raw/a7e61654553887475a2f7178108587ecd9b54608/data/delete_me/malt/test.rma6', checkIfExists: true)
]
megan_summary = true
MEGAN_RMA2INFO ( input, megan_summary )
}

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process {
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
withName: MEGAN_RMA2INFO {
ext.args = "-c2c Taxonomy"
}
}

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@ -0,0 +1,12 @@
- name: megan rma2info test_megan_rma2info
command: nextflow run tests/modules/megan/rma2info -entry test_megan_rma2info -c tests/config/nextflow.config
tags:
- megan
- megan/rma2info
files:
- path: output/megan/test.megan
contains:
- "@Creator"
- path: output/megan/test.txt.gz
md5sum: 5c3b876aa0abef12158bcd7c3702740f
- path: output/megan/versions.yml