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Bcftools sort new syntax (#1169)
* Added files for bcftools/sort * Fixed output format and removed md5 checksum * Change input to avoid identical in/output names * Created rows for bcftools/sort in pytest_modules.yml
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32
modules/bcftools/sort/main.nf
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32
modules/bcftools/sort/main.nf
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process BCFTOOLS_SORT {
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tag "$meta.id"
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label 'process_medium'
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conda (params.enable_conda ? "bioconda::bcftools=1.14" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/bcftools:1.14--h88f3f91_0':
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'quay.io/biocontainers/bcftools:1.14--h88f3f91_0' }"
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input:
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tuple val(meta), path(vcf)
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output:
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tuple val(meta), path("*.gz"), emit: vcf
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path "versions.yml" , emit: versions
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script:
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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"""
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bcftools \\
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sort \\
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--output ${prefix}.vcf.gz \\
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$args \\
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$vcf
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
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END_VERSIONS
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"""
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}
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43
modules/bcftools/sort/meta.yml
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modules/bcftools/sort/meta.yml
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name: bcftools_sort
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description: Sorts VCF files
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keywords:
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- sorting
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- VCF
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- variant calling
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tools:
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- sort:
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description: Sort VCF files by coordinates.
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homepage: http://samtools.github.io/bcftools/bcftools.html
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documentation: http://www.htslib.org/doc/bcftools.html
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tool_dev_url: https://github.com/samtools/bcftools
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doi: "10.1093/bioinformatics/btp352"
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licence: ['MIT']
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input:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- vcf:
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type: file
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description: The VCF/BCF file to be sorted
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pattern: "*.{vcf.gz,vcf,bcf}"
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output:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- versions:
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type: file
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description: File containing software versions
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pattern: "versions.yml"
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- vcf:
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type: file
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description: Sorted VCF file
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pattern: "*.{vcf.gz}"
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authors:
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- "@Gwennid"
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@ -114,6 +114,10 @@ bcftools/reheader:
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- modules/bcftools/reheader/**
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- tests/modules/bcftools/reheader/**
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bcftools/sort:
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- modules/bcftools/sort/**
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- tests/modules/bcftools/sort/**
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bcftools/stats:
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- modules/bcftools/stats/**
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- tests/modules/bcftools/stats/**
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15
tests/modules/bcftools/sort/main.nf
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15
tests/modules/bcftools/sort/main.nf
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#!/usr/bin/env nextflow
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nextflow.enable.dsl = 2
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include { BCFTOOLS_SORT } from '../../../../modules/bcftools/sort/main.nf'
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workflow test_bcftools_sort {
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input = [
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[ id:'test' ], // meta map
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file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true)
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]
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BCFTOOLS_SORT ( input )
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}
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5
tests/modules/bcftools/sort/nextflow.config
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5
tests/modules/bcftools/sort/nextflow.config
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process {
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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}
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10
tests/modules/bcftools/sort/test.yml
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10
tests/modules/bcftools/sort/test.yml
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- name: bcftools sort test_bcftools_sort
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command: nextflow run tests/modules/bcftools/sort -entry test_bcftools_sort -c tests/config/nextflow.config
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tags:
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- bcftools
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- bcftools/sort
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files:
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- path: output/bcftools/test.vcf.gz
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md5sum: fc178eb342a91dc0d1d568601ad8f8e2
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- path: output/bcftools/versions.yml
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md5sum: f1bb553befa5108f81135b8fec613b6b
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