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hmtnote
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82
modules/hmtnote/main.nf
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82
modules/hmtnote/main.nf
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// TODO nf-core: If in doubt look at other nf-core/modules to see how we are doing things! :)
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// https://github.com/nf-core/modules/tree/master/modules
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// You can also ask for help via your pull request or on the #modules channel on the nf-core Slack workspace:
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// https://nf-co.re/join
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// TODO nf-core: A module file SHOULD only define input and output files as command-line parameters.
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// All other parameters MUST be provided using the "task.ext" directive, see here:
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// https://www.nextflow.io/docs/latest/process.html#ext
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// where "task.ext" is a string.
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// Any parameters that need to be evaluated in the context of a particular sample
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// e.g. single-end/paired-end data MUST also be defined and evaluated appropriately.
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// TODO nf-core: Software that can be piped together SHOULD be added to separate module files
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// unless there is a run-time, storage advantage in implementing in this way
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// e.g. it's ok to have a single module for bwa to output BAM instead of SAM:
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// bwa mem | samtools view -B -T ref.fasta
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// TODO nf-core: Optional inputs are not currently supported by Nextflow. However, using an empty
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// list (`[]`) instealdd of a file can be used to work around this issue.
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process HMTNOTE {
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tag "$meta.id"
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label 'process_low'
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// TODO nf-core: List required Conda package(s).
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// Software MUST be pinned to channel (i.e. "bioconda"), version (i.e. "1.10").
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// For Conda, the build (i.e. "h9402c20_2") must be EXCLUDED to support installation on different operating systems.
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// TODO nf-core: See section in main README for further information regarding finding and adding container addresses to the section below.
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conda (params.enable_conda ? "bioconda::hmtnote=0.7.2" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/hmtnote:0.7.2--pyhdfd78af_0':
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'quay.io/biocontainers/hmtnote:0.7.2--pyhdfd78af_0' }"
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input:
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// TODO nf-core: Where applicable all sample-specific information e.g. "id", "single_end", "read_group"
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// MUST be provided as an input via a Groovy Map called "meta".
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// This information may not be required in some instances e.g. indexing reference genome files:
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// https://github.com/nf-core/modules/blob/master/modules/bwa/index/main.nf
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// TODO nf-core: Where applicable please provide/convert compressed files as input/output
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// e.g. "*.fastq.gz" and NOT "*.fastq", "*.bam" and NOT "*.sam" etc.
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tuple val(meta), path(vcf)
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output:
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// TODO nf-core: Named file extensions MUST be emitted for ALL output channels
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tuple val(meta), path("*_annotated.vcf"), emit: vcf
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// TODO nf-core: List additional required output channels/values here
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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// TODO nf-core: Where possible, a command MUST be provided to obtain the version number of the software e.g. 1.10
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// If the software is unable to output a version number on the command-line then it can be manually specified
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// e.g. https://github.com/nf-core/modules/blob/master/modules/homer/annotatepeaks/main.nf
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// Each software used MUST provide the software name and version number in the YAML version file (versions.yml)
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// TODO nf-core: It MUST be possible to pass additional parameters to the tool as a command-line string via the "task.ext.args" directive
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// TODO nf-core: If the tool supports multi-threading then you MUST provide the appropriate parameter
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// using the Nextflow "task" variable e.g. "--threads $task.cpus"
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// TODO nf-core: Please replace the example samtools command below with your module's command
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// TODO nf-core: Please indent the command appropriately (4 spaces!!) to help with readability ;)
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"""
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hmtnote \\
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annotate \\
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$vcf \\
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${prefix}_annotated.vcf \\
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$args
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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hmtnote: \$(echo \$(hmtnote --version 2>&1) | sed 's/^.*hmtnote //; s/Using.*\$//' ))
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END_VERSIONS
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"""
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stub:
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def prefix = task.ext.prefix ?: "${meta.id}"
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"""
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touch ${prefix}_annotated.vcf
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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hmtnote: \$(echo \$(hmtnote --version 2>&1) | sed 's/^.*hmtnote //; s/Using.*\$//' ))
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END_VERSIONS
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"""
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}
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37
modules/hmtnote/meta.yml
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modules/hmtnote/meta.yml
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name: hmtnote
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## TODO nf-core: Add a description of the module and list keywords
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description: Human mitochondrial variants annotation using HmtVar.
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keywords:
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- sort
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tools:
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- hmtnote:
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## TODO nf-core: Add a description and other details for the software below
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description: Human mitochondrial variants annotation using HmtVar.
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homepage: None
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documentation: None
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tool_dev_url: None
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doi: ""
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licence: ['MIT']
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## TODO nf-core: Add a description of all of the variables used as input
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input:
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## TODO nf-core: Delete / customise this example input
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- vcf:
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type: file
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description: vcf file
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pattern: "*.{vcf}"
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## TODO nf-core: Add a description of all of the variables used as output
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output:
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- versions:
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type: file
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description: File containing software versions
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pattern: "versions.yml"
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## TODO nf-core: Delete / customise this example output
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- vcf:
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type: file
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description: annotated vcf
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pattern: "*_annotated.vcf"
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authors:
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- "@sysbiocoder"
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14
tests/modules/hmtnote/main.nf
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14
tests/modules/hmtnote/main.nf
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#!/usr/bin/env nextflow
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nextflow.enable.dsl = 2
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include { HMTNOTE } from '../../../modules/hmtnote/main.nf'
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workflow test_hmtnote {
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input = [ [ id:'test' ], // meta map
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file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true)
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]
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HMTNOTE ( input )
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}
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7
tests/modules/hmtnote/nextflow.config
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7
tests/modules/hmtnote/nextflow.config
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process {
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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withName: HMTNOTE {
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ext.args = '--basic --variab'
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}
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}
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9
tests/modules/hmtnote/test.yml
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9
tests/modules/hmtnote/test.yml
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## TODO nf-core: Please run the following command to build this file:
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# nf-core modules create-test-yml hmtnote
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- name: hmtnote
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command: nextflow run ./tests/modules/hmtnote -entry test_hmtnote -c ./tests/config/nextflow.config -c ./tests/modules/hmtnote/nextflow.config
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tags:
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- hmtnote
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files:
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- path: output/vcfanno/test_annotated.vcf
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md5sum: 90ceb2fd2e06e781846d69c3981db665
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