diff --git a/tests/modules/bamtools/split/main.nf b/tests/modules/bamtools/split/main.nf
index ff0cb748..9787b4ed 100644
--- a/tests/modules/bamtools/split/main.nf
+++ b/tests/modules/bamtools/split/main.nf
@@ -20,7 +20,7 @@ workflow test_bamtools_split_multiple {
     input = [
         [ id:'test', single_end:false ], // meta map
         [file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
-        file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)]
+        file(params.test_data['homo_sapiens']['illumina']['test2_paired_end_sorted_bam'], checkIfExists: true)]
 
     ]
 
diff --git a/tests/modules/bamtools/split/test.yml b/tests/modules/bamtools/split/test.yml
index b52cc9ee..63ecac57 100644
--- a/tests/modules/bamtools/split/test.yml
+++ b/tests/modules/bamtools/split/test.yml
@@ -1,5 +1,5 @@
-- name: bamtools split test_bamtools_split
-  command: nextflow run tests/modules/bamtools/split -entry test_bamtools_split -c tests/config/nextflow.config
+- name: bamtools split test_bamtools_split_single_input
+  command: nextflow run tests/modules/bamtools/split -entry test_bamtools_split_single_input -c tests/config/nextflow.config
   tags:
     - bamtools
     - bamtools/split
@@ -9,3 +9,15 @@
     - path: output/bamtools/test.REF_unmapped.bam
       md5sum: e0754bf72c51543b2d745d96537035fb
     - path: output/bamtools/versions.yml
+
+- name: bamtools split test_bamtools_split_multiple
+  command: nextflow run tests/modules/bamtools/split -entry test_bamtools_split_multiple -c tests/config/nextflow.config
+  tags:
+    - bamtools
+    - bamtools/split
+  files:
+    - path: output/bamtools/test.REF_chr22.bam
+      md5sum: 585675bea34c48ebe9db06a561d4b4fa
+    - path: output/bamtools/test.REF_unmapped.bam
+      md5sum: 16ad644c87b9471f3026bc87c98b4963
+    - path: output/bamtools/versions.yml