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https://github.com/MillironX/nf-core_modules.git
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add stub and the option to compress output for vep (#2111)
* add stub * create tab and json files * add option to get compressed outputs
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parent
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commit
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4 changed files with 85 additions and 6 deletions
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@ -17,11 +17,14 @@ process ENSEMBLVEP {
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path extra_files
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output:
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tuple val(meta), path("*.ann.vcf") , optional:true, emit: vcf
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tuple val(meta), path("*.ann.tab") , optional:true, emit: tab
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tuple val(meta), path("*.ann.json") , optional:true, emit: json
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path "*.summary.html" , emit: report
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path "versions.yml" , emit: versions
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tuple val(meta), path("*.ann.vcf") , optional:true, emit: vcf
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tuple val(meta), path("*.ann.tab") , optional:true, emit: tab
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tuple val(meta), path("*.ann.json") , optional:true, emit: json
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tuple val(meta), path("*.ann.vcf.gz") , optional:true, emit: vcf_gz
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tuple val(meta), path("*.ann.tab.gz") , optional:true, emit: tab_gz
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tuple val(meta), path("*.ann.json.gz") , optional:true, emit: json_gz
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path "*.summary.html" , emit: report
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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@ -29,6 +32,7 @@ process ENSEMBLVEP {
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script:
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def args = task.ext.args ?: ''
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def file_extension = args.contains("--vcf") ? 'vcf' : args.contains("--json")? 'json' : args.contains("--tab")? 'tab' : 'vcf'
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def compress_out = args.contains("--compress_output") ? '.gz' : ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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def dir_cache = cache ? "\${PWD}/${cache}" : "/.vep"
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def reference = fasta ? "--fasta $fasta" : ""
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@ -36,7 +40,7 @@ process ENSEMBLVEP {
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"""
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vep \\
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-i $vcf \\
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-o ${prefix}.ann.${file_extension} \\
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-o ${prefix}.ann.${file_extension}${compress_out} \\
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$args \\
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$reference \\
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--assembly $genome \\
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@ -48,6 +52,23 @@ process ENSEMBLVEP {
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--stats_file ${prefix}.summary.html \\
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//')
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END_VERSIONS
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"""
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stub:
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def prefix = task.ext.prefix ?: "${meta.id}"
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"""
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touch ${prefix}.ann.vcf
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touch ${prefix}.ann.tab
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touch ${prefix}.ann.json
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touch ${prefix}.ann.vcf.gz
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touch ${prefix}.ann.tab.gz
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touch ${prefix}.ann.json.gz
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touch ${prefix}.summary.html
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//')
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@ -7,6 +7,8 @@ include { ENSEMBLVEP } from '../../../modules/ensemblvep/main.nf'
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include { ENSEMBLVEP as ENSEMBLVEP_JSON } from '../../../modules/ensemblvep/main.nf'
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include { ENSEMBLVEP as ENSEMBLVEP_TAB } from '../../../modules/ensemblvep/main.nf'
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include { ENSEMBLVEP as ENSEMBLVEP_VCF } from '../../../modules/ensemblvep/main.nf'
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include { ENSEMBLVEP as ENSEMBLVEP_VCF_BGZIP } from '../../../modules/ensemblvep/main.nf'
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include { ENSEMBLVEP as ENSEMBLVEP_VCF_GZIP } from '../../../modules/ensemblvep/main.nf'
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workflow test_ensemblvep_fasta_json {
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input = [
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@ -41,6 +43,28 @@ workflow test_ensemblvep_fasta_vcf {
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ENSEMBLVEP_VCF ( input, "WBcel235", "caenorhabditis_elegans", "106", [], fasta, [] )
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}
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workflow test_ensemblvep_fasta_vcf_bgzip {
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input = [
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[ id:'test' ], // meta map
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file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true)
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]
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fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
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ENSEMBLVEP_VCF_BGZIP ( input, "WBcel235", "caenorhabditis_elegans", "106", [], fasta, [] )
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}
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workflow test_ensemblvep_fasta_vcf_gzip {
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input = [
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[ id:'test' ], // meta map
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file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true)
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]
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fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
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ENSEMBLVEP_VCF_GZIP ( input, "WBcel235", "caenorhabditis_elegans", "106", [], fasta, [] )
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}
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workflow test_ensemblvep_fasta {
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input = [
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[ id:'test' ], // meta map
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@ -20,4 +20,14 @@ process {
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container = 'nfcore/vep:106.1.WBcel235'
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ext.args = '--vcf'
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}
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withName: ENSEMBLVEP_VCF_BGZIP {
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container = 'nfcore/vep:106.1.WBcel235'
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ext.args = '--vcf --compress_output bgzip'
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}
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withName: ENSEMBLVEP_VCF_GZIP {
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container = 'nfcore/vep:106.1.WBcel235'
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ext.args = '--vcf --compress_output gzip'
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}
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}
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@ -22,6 +22,22 @@
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- path: output/ensemblvep/test.ann.vcf
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- path: output/ensemblvep/test.summary.html
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- name: ensemblvep test_ensemblvep_fasta_vcf_bgzip
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command: nextflow run ./tests/modules/ensemblvep -entry test_ensemblvep_fasta_vcf_bgzip -c ./tests/config/nextflow.config -c ./tests/modules/ensemblvep/nextflow.config
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tags:
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- ensemblvep
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files:
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- path: output/ensemblvep/test.ann.vcf.gz
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- path: output/ensemblvep/test.summary.html
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- name: ensemblvep test_ensemblvep_fasta_vcf_gzip
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command: nextflow run ./tests/modules/ensemblvep -entry test_ensemblvep_fasta_vcf_gzip -c ./tests/config/nextflow.config -c ./tests/modules/ensemblvep/nextflow.config
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tags:
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- ensemblvep
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files:
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- path: output/ensemblvep/test.ann.vcf.gz
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- path: output/ensemblvep/test.summary.html
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- name: ensemblvep test_ensemblvep_fasta
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command: nextflow run ./tests/modules/ensemblvep -entry test_ensemblvep_fasta -c ./tests/config/nextflow.config -c ./tests/modules/ensemblvep/nextflow.config
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tags:
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@ -37,3 +53,11 @@
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files:
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- path: output/ensemblvep/test.ann.vcf
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- path: output/ensemblvep/test.summary.html
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- name: ensemblvep test_ensemblvep_fasta_vcf_stub
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command: nextflow run ./tests/modules/ensemblvep -entry test_ensemblvep_fasta_vcf -c ./tests/config/nextflow.config -c ./tests/modules/ensemblvep/nextflow.config -stub
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tags:
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- ensemblvep
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files:
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- path: output/ensemblvep/test.ann.vcf
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- path: output/ensemblvep/test.summary.html
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