diff --git a/tests/software/bowtie/align/test.yml b/tests/software/bowtie/align/test.yml
index a941b233..add90d7c 100644
--- a/tests/software/bowtie/align/test.yml
+++ b/tests/software/bowtie/align/test.yml
@@ -42,6 +42,6 @@
     - path: output/bowtie/bowtie/NC_010473.rev.2.ebwt
       md5sum: f3c398bba5158f4039334a932d79c051
     - path: output/bowtie/test.out
-      md5sum: a23e9a2a76e949aeb3693bcfae41a615
+      md5sum: a81cb18024616415a6cec3108a36fccd
     - path: output/bowtie/test.bam
       should_exist: true
\ No newline at end of file