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Add GATK indelrealigner tests
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commit
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4 changed files with 35 additions and 26 deletions
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@ -22,11 +22,12 @@ process GATK_INDELREALIGNER {
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task.ext.when == null || task.ext.when
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task.ext.when == null || task.ext.when
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script:
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script:
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if ("$input" == "${prefix}.bam") error "Input and output names are the same, set prefix in module configuration to disambiguate!"
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def args = task.ext.args ?: ''
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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def prefix = task.ext.prefix ?: "${meta.id}"
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def known = known_vcf ? "-known ${known_vcf}" : ""
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def known = known_vcf ? "-known ${known_vcf}" : ""
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if ("$input" == "${prefix}.bam") error "Input and output names are the same, set prefix in module configuration to disambiguate!"
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def avail_mem = 3
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def avail_mem = 3
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if (!task.memory) {
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if (!task.memory) {
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log.info '[GATK IndelRealigner] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
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log.info '[GATK IndelRealigner] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
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@ -39,9 +40,8 @@ process GATK_INDELREALIGNER {
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-Xmx${avail_mem}g \\
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-Xmx${avail_mem}g \\
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-T IndelRealigner \\
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-T IndelRealigner \\
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-R ${fasta} \\
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-R ${fasta} \\
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-nt ${task.cpus}
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-I ${input} \\
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-I ${input} \\
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-targetIntervals ${intervals} \\
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--targetIntervals ${intervals} \\
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${known} \\
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${known} \\
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-o ${prefix}.bam \\
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-o ${prefix}.bam \\
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$args
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$args
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@ -2,21 +2,32 @@
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nextflow.enable.dsl = 2
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nextflow.enable.dsl = 2
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include { GATK_REALIGNERTARGETCREATOR } from '../../../../modules/gatk/realignertargetcreator/main.nf'
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include { GATK_INDELREALIGNER } from '../../../../modules/gatk/indelrealigner/main.nf'
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include { GATK_INDELREALIGNER } from '../../../../modules/gatk/indelrealigner/main.nf'
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// TODO add REalignerTargetCrator
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workflow test_gatk_indelrealigner {
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workflow test_gatk_indelrealigner {
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input = [
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[ id:'test', single_end:false ], // meta map
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fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
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file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true),
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fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
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file(params.test_data['sarscov2']['illumina']['test_paired_end_bai'], checkIfExists: true),
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dict = file(params.test_data['sarscov2']['genome']['genome_dict'], checkIfExists: true)
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GATK_REALIGNERTARGETCREATOR.out.intervals
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input_realignertargetcreator = [ [ id:'test' ], // meta map
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file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
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file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true),
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]
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]
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reference = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
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GATK_REALIGNERTARGETCREATOR ( input_realignertargetcreator, fasta, fai, dict, [] )
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GATK_INDELREALIGNER ( input, reference, [] )
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ch_intervals = GATK_REALIGNERTARGETCREATOR.out.intervals
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ch_bams_indelrealigner = Channel.of([ [ id:'test' ], // meta map
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file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
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file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
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])
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ch_input_indelrealigner = ch_bams_indelrealigner.mix(ch_intervals).groupTuple(by: 0).map{ [it[0], it[1][0], it[2], it[1][1] ] }.dump(tag: "input")
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GATK_INDELREALIGNER ( ch_input_indelrealigner, fasta, fai, dict, [] )
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}
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}
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@ -1,6 +1,6 @@
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process {
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process {
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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ext.prefix = "${meta.id}.realigned"
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ext.prefix = { "${meta.id}.realigned" }
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}
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}
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@ -1,14 +1,12 @@
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## TODO nf-core: Please run the following command to build this file:
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- name: gatk indelrealigner test_gatk_indelrealigner
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# nf-core modules create-test-yml gatk/indelrealigner
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- name: "gatk indelrealigner"
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command: nextflow run ./tests/modules/gatk/indelrealigner -entry test_gatk_indelrealigner -c ./tests/config/nextflow.config -c ./tests/modules/gatk/indelrealigner/nextflow.config
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command: nextflow run ./tests/modules/gatk/indelrealigner -entry test_gatk_indelrealigner -c ./tests/config/nextflow.config -c ./tests/modules/gatk/indelrealigner/nextflow.config
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tags:
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tags:
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- "gatk"
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- gatk/indelrealigner
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#
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- gatk
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- "gatk/indelrealigner"
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#
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files:
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files:
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- path: "output/gatk/test.bam"
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- path: output/gatk/test.realigned.bai
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md5sum: e667c7caad0bc4b7ac383fd023c654fc
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md5sum: 85a67df8827fe426e7f3a458134c0551
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- path: output/gatk/versions.yml
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- path: output/gatk/test.realigned.bam
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md5sum: a01fe51bc4c6a3a6226fbf77b2c7cf3b
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md5sum: ea1df6f7fcafc408fae4dc1574813d8a
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- path: output/gatk/test.realigned.intervals
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md5sum: 7aa7a1b235a510e6591e262382086bf8
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