Merge pull request #45 from FelixKrueger/master

Adding back in all input files we had before
This commit is contained in:
Harshil Patel 2020-07-16 09:32:06 +01:00 committed by GitHub
commit 4ee6da3a34
No known key found for this signature in database
GPG key ID: 4AEE18F83AFDEB23
74 changed files with 74562 additions and 0 deletions

Binary file not shown.

Binary file not shown.

Binary file not shown.

Binary file not shown.

Binary file not shown.

Binary file not shown.

View file

@ -0,0 +1 @@
../../../../../tests/data/fasta/E_coli/NC_010473.fa

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/dna/Ecoli_DNA_R1.fastq.gz

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/dna/Ecoli_DNA_R2.fastq.gz

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R1_val_1.fq.gz

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R2_val_2.fq.gz

View file

@ -0,0 +1,15 @@
10000 reads; of these:
10000 (100.00%) were paired; of these:
893 (8.93%) aligned concordantly 0 times
8474 (84.74%) aligned concordantly exactly 1 time
633 (6.33%) aligned concordantly >1 times
----
893 pairs aligned concordantly 0 times; of these:
815 (91.27%) aligned discordantly 1 time
----
78 pairs aligned 0 times concordantly or discordantly; of these:
156 mates make up the pairs; of these:
0 (0.00%) aligned 0 times
1 (0.64%) aligned exactly 1 time
155 (99.36%) aligned >1 times
100.00% overall alignment rate

Binary file not shown.

View file

@ -0,0 +1,15 @@
9979 reads; of these:
9979 (100.00%) were paired; of these:
3584 (35.92%) aligned concordantly 0 times
3705 (37.13%) aligned concordantly exactly 1 time
2690 (26.96%) aligned concordantly >1 times
----
3584 pairs aligned concordantly 0 times; of these:
886 (24.72%) aligned discordantly 1 time
----
2698 pairs aligned 0 times concordantly or discordantly; of these:
5396 mates make up the pairs; of these:
2282 (42.29%) aligned 0 times
1467 (27.19%) aligned exactly 1 time
1647 (30.52%) aligned >1 times
88.57% overall alignment rate

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R1.fastq.gz

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R1_val_1.fq.gz

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R2.fastq.gz

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R2_val_2.fq.gz

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_single_end.fastq.gz

View file

@ -0,0 +1,31 @@
#Fastq_screen version: 0.14.0 #Aligner: bowtie2 #Reads in subset: 100000
Genome #Reads_processed #Unmapped %Unmapped #One_hit_one_genome %One_hit_one_genome #Multiple_hits_one_genome %Multiple_hits_one_genome #One_hit_multiple_genomes %One_hit_multiple_genomes Multiple_hits_multiple_genomes %Multiple_hits_multiple_genomes
Cat 10000 9171 91.71 0 0.00 0 0.00 421 4.21 408 4.08
Chicken 10000 8932 89.32 0 0.00 0 0.00 64 0.64 1004 10.04
Cow 10000 8484 84.84 0 0.00 0 0.00 294 2.94 1222 12.22
Drosophila 10000 9469 94.69 0 0.00 0 0.00 19 0.19 512 5.12
Human 10000 8367 83.67 2 0.02 3 0.03 354 3.54 1274 12.74
Mouse 10000 122 1.22 3265 32.65 869 8.69 2066 20.66 3678 36.78
Pig 10000 8459 84.59 0 0.00 0 0.00 334 3.34 1207 12.07
Rat 10000 6432 64.32 1 0.01 3 0.03 1334 13.34 2230 22.30
Zebrafish 10000 9125 91.25 0 0.00 0 0.00 41 0.41 834 8.34
Arabidopsis 10000 9497 94.97 0 0.00 0 0.00 5 0.05 498 4.98
Grape 10000 9600 96.00 0 0.00 1 0.01 82 0.82 317 3.17
Potato 10000 9460 94.60 0 0.00 0 0.00 12 0.12 528 5.28
Tomato 10000 9521 95.21 0 0.00 0 0.00 45 0.45 434 4.34
Adapters 10000 10000 100.00 0 0.00 0 0.00 0 0.00 0 0.00
Brachybacterium 10000 10000 100.00 0 0.00 0 0.00 0 0.00 0 0.00
Pseudomonas 10000 10000 100.00 0 0.00 0 0.00 0 0.00 0 0.00
Massilia_oculi 10000 9999 99.99 0 0.00 1 0.01 0 0.00 0 0.00
Ecoli 10000 9998 99.98 1 0.01 1 0.01 0 0.00 0 0.00
Lambda 10000 10000 100.00 0 0.00 0 0.00 0 0.00 0 0.00
MT 10000 7856 78.56 0 0.00 0 0.00 2034 20.34 110 1.10
PhiX 10000 10000 100.00 0 0.00 0 0.00 0 0.00 0 0.00
rRNA 10000 9157 91.57 0 0.00 0 0.00 111 1.11 732 7.32
Wasp 10000 9473 94.73 0 0.00 0 0.00 211 2.11 316 3.16
Vectors 10000 9713 97.13 0 0.00 0 0.00 52 0.52 235 2.35
Worm 10000 9645 96.45 0 0.00 0 0.00 13 0.13 342 3.42
Yeast 10000 9507 95.07 0 0.00 0 0.00 4 0.04 489 4.89
Mycoplasma 10000 9998 99.98 0 0.00 0 0.00 0 0.00 2 0.02
%Hit_no_genomes: 0.88

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R1_val_1.fq.gz

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R2_val_2.fq.gz

File diff suppressed because one or more lines are too long

Binary file not shown.

File diff suppressed because one or more lines are too long

Binary file not shown.

File diff suppressed because one or more lines are too long

Binary file not shown.

File diff suppressed because one or more lines are too long

Binary file not shown.

Binary file not shown.

Binary file not shown.

Binary file not shown.

Binary file not shown.

Binary file not shown.

Binary file not shown.

Binary file not shown.

Binary file not shown.

View file

@ -0,0 +1 @@
../../../../../tests/data/fasta/E_coli/NC_010473.fa

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/dna/Ecoli_DNA_R1.fastq.gz

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/dna/Ecoli_DNA_R2.fastq.gz

View file

@ -0,0 +1,6 @@
10000 reads; of these:
10000 (100.00%) were paired; of these:
823 (8.23%) aligned concordantly 0 times
8583 (85.83%) aligned concordantly exactly 1 time
594 (5.94%) aligned concordantly >1 times
91.77% overall alignment rate

View file

@ -0,0 +1 @@
../../../../bowtie2/test/output/test_GRCm38_bowtie2_stats.txt

View file

@ -0,0 +1 @@
../../../../fastq_screen/test/output/test_R1_screen.txt

View file

@ -0,0 +1 @@
../../../../fastqc/test/output/test_R1_fastqc.zip

View file

@ -0,0 +1 @@
../../../../fastqc/test/output/test_R1_val_1_fastqc.zip

View file

@ -0,0 +1 @@
../../../../fastqc/test/output/test_R2_fastqc.zip

View file

@ -0,0 +1 @@
../../../../fastqc/test/output/test_R2_val_2_fastqc.zip

View file

@ -0,0 +1 @@
../../../../hisat2/test/output/Ecoli_DNA_R_E_coli_hisat2_stats.txt

View file

@ -0,0 +1 @@
../../../../trim_galore/test/output/test_R1.fastq.gz_trimming_report.txt

View file

@ -0,0 +1 @@
../../../../trim_galore/test/output/test_R2.fastq.gz_trimming_report.txt

File diff suppressed because one or more lines are too long

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R1.fastq.gz

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R2.fastq.gz

View file

@ -0,0 +1,97 @@
SUMMARISING RUN PARAMETERS
==========================
Input filename: test_R1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.6.5
Cutadapt version: 2.3
Number of cores used for trimming: 1
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Using Nextera adapter for trimming (count: 83). Second best hit was smallRNA (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Output file will be GZIP compressed
This is cutadapt 2.3 with Python 3.7.3
Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA test_R1.fastq.gz
Processing reads on 1 core in single-end mode ...
Finished in 0.19 s (19 us/read; 3.12 M reads/minute).
=== Summary ===
Total reads processed: 10,000
Reads with adapters: 3,225 (32.2%)
Reads written (passing filters): 10,000 (100.0%)
Total basepairs processed: 760,000 bp
Quality-trimmed: 4,492 bp (0.6%)
Total written (filtered): 748,403 bp (98.5%)
=== Adapter 1 ===
Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 3225 times.
No. of allowed errors:
0-9 bp: 0; 10-12 bp: 1
Bases preceding removed adapters:
A: 23.8%
C: 28.2%
G: 22.7%
T: 25.3%
none/other: 0.0%
Overview of removed sequences
length count expect max.err error counts
1 2170 2500.0 0 2170
2 622 625.0 0 622
3 223 156.2 0 223
4 64 39.1 0 64
5 14 9.8 0 14
6 9 2.4 0 9
7 8 0.6 0 8
8 5 0.2 0 5
9 4 0.0 0 4
10 8 0.0 1 7 1
11 3 0.0 1 3
12 4 0.0 1 4
13 6 0.0 1 6
14 5 0.0 1 4 1
15 5 0.0 1 5
16 6 0.0 1 5 1
17 3 0.0 1 3
18 3 0.0 1 3
19 1 0.0 1 1
20 3 0.0 1 3
21 7 0.0 1 7
22 7 0.0 1 7
23 3 0.0 1 3
24 6 0.0 1 6
25 4 0.0 1 4
26 2 0.0 1 2
27 4 0.0 1 4
28 1 0.0 1 1
29 3 0.0 1 3
30 4 0.0 1 4
32 3 0.0 1 3
33 2 0.0 1 1 1
34 1 0.0 1 1
35 1 0.0 1 1
40 1 0.0 1 1
42 1 0.0 1 0 1
45 1 0.0 1 0 1
49 1 0.0 1 0 1
52 1 0.0 1 0 1
56 2 0.0 1 0 2
59 1 0.0 1 0 1
67 1 0.0 1 0 1
70 2 0.0 1 0 2
RUN STATISTICS FOR INPUT FILE: test_R1.fastq.gz
=============================================
10000 sequences processed in total

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R1_val_1.fq.gz

View file

@ -0,0 +1,100 @@
SUMMARISING RUN PARAMETERS
==========================
Input filename: test_R2.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.6.5
Cutadapt version: 2.3
Number of cores used for trimming: 1
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Using Nextera adapter for trimming (count: 83). Second best hit was smallRNA (count: 0)
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Output file will be GZIP compressed
This is cutadapt 2.3 with Python 3.7.3
Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA test_R2.fastq.gz
Processing reads on 1 core in single-end mode ...
Finished in 0.22 s (22 us/read; 2.71 M reads/minute).
=== Summary ===
Total reads processed: 10,000
Reads with adapters: 3,295 (33.0%)
Reads written (passing filters): 10,000 (100.0%)
Total basepairs processed: 760,000 bp
Quality-trimmed: 7,096 bp (0.9%)
Total written (filtered): 745,649 bp (98.1%)
=== Adapter 1 ===
Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 3295 times.
No. of allowed errors:
0-9 bp: 0; 10-12 bp: 1
Bases preceding removed adapters:
A: 22.6%
C: 28.2%
G: 23.6%
T: 25.6%
none/other: 0.0%
Overview of removed sequences
length count expect max.err error counts
1 2213 2500.0 0 2213
2 647 625.0 0 647
3 239 156.2 0 239
4 53 39.1 0 53
5 10 9.8 0 10
6 7 2.4 0 7
7 8 0.6 0 8
8 5 0.2 0 5
9 5 0.0 0 5
10 10 0.0 1 8 2
11 2 0.0 1 2
12 4 0.0 1 4
13 7 0.0 1 7
14 3 0.0 1 3
15 4 0.0 1 4
16 5 0.0 1 5
17 3 0.0 1 3
18 5 0.0 1 4 1
19 2 0.0 1 1 1
20 3 0.0 1 3
21 7 0.0 1 7
22 6 0.0 1 6
23 3 0.0 1 3
24 7 0.0 1 7
25 4 0.0 1 4
26 2 0.0 1 2
27 4 0.0 1 4
28 1 0.0 1 1
29 3 0.0 1 3
30 4 0.0 1 4
32 3 0.0 1 3
33 1 0.0 1 1
34 1 0.0 1 1
35 2 0.0 1 1 1
40 1 0.0 1 0 1
41 1 0.0 1 1
46 1 0.0 1 0 1
48 1 0.0 1 0 1
49 2 0.0 1 0 2
56 2 0.0 1 0 2
59 1 0.0 1 0 1
70 1 0.0 1 0 1
73 2 0.0 1 0 2
RUN STATISTICS FOR INPUT FILE: test_R2.fastq.gz
=============================================
10000 sequences processed in total
Total number of sequences analysed for the sequence pair length validation: 10000
Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 21 (0.21%)

View file

@ -0,0 +1 @@
../../../../tests/data/fastq/rna/test_R2_val_2.fq.gz

File diff suppressed because it is too large Load diff

View file

@ -0,0 +1,265 @@
>seq001
MAGKRKRANAPDQTERRSSVRVQKVRQKALDEKARLVQERVKLLSDRKSEICVDDTELHE
KEEENVDGSPKRRSPPKLTAMQKGKQKLSVSLNGKDVNLEPHLKVTKCLRLFNKQYLLCV
QAKLSRPDLKGVTEMIKAKAILYPRKIIGDLPGIDVGHRFFSRAEMCAVGFHNHWLNGID
YMSMEYEKEYSNYKLPLAVSIVMSGQYEDDLDNADTVTYTGQGGHNLTGNKRQIKDQLLE
RGNLALKHCCEYNVPVRVTRGHNCKSSYTKRVYTYDGLYKVEKFWAQKGVSGFTVYKYRL
KRLEGQPELTTDQVNFVAGRIPTSTSEIEGLVCEDISGGLEFKGIPATNRVDDSPVSPTS
GFTYIKSLIIEPNVIIPKSSTGCNCRGSCTDSKKCACAKLNGGNFPYVDLNDGRLIESRD
VVFECGPHCGCGPKCVNRTSQKRLRFNLEVFRSAKKGWAVRSWEYIPAGSPVCEYIGVVR
RTADVDTISDNEYIFEIDCQQTMQGLGGRQRRLRDVAVPMNNGVSQSSEDENAPEFCIDA
GSTGNFARFINHSCEPNLFVQCVLSSHQDIRLARVVLFAADNISPMQELTYDYGYALDSV
HGPDGKVKQLACYCGALNCRKRLY
>seq002
MPRHFGAVPGVVPGMAFVNRQELRDAGVHLPTQAGISGSASEGADSIVLSGGYEDDRDEG
DVILYTGEGGRDPLTGHQVKPQQLVRGNLALAISHRDGLPLRVTRGHRHSSQFSPQSGYQ
YAGLYRVDDHWREVGRSGFLIWRFRLTRLENQDAHHAGADPQHPDSQHPERRPTLVQRIV
RDTATARAVKALYDHRCQVCGERLETPAGAYAEAAHIRPLGAPHHGPDVAGNILCLCPNH
HVLFDFGAFSVGDDLRLLGLPGRLHVHPQHAVDREHLAYHRRHYALQAGLEWGCSVPLT
>seq003
MGVMENLMVHTEISKVKSQSNGEVEKRGVSVLENGGVCKLDRMSGLKFKRRKVFAVRDFP
PGCGSRAMEVKIACENGNVVEDVKVVESLVKEEESLGQRDASENVSDIRMAEPVEVQPLR
ICLPGGDVVRDLSVTAGDECSNSEQIVAGSGVSSSSGTENIVRDIVVYADESSLGMDNLD
QTQPLEIEMSDVAVAKPRLVAGRKKAKKGIACHSSLKVVSREFGEGSRKKKSKKNLYWRD
RESLDSPEQLRILGVGTSSGSSSGDSSRNKVKETLRLFHGVCRKILQEDEAKPEDQRRKG
KGLRIDFEASTILKRNGKFLNSGVHILGEVPGVEVGDEFQYRMELNILGIHKPSQAGIDY
MKYGKAKVATSIVASGGYDDHLDNSDVLTYTGQGGNVMQVKKKGEELKEPEDQKLITGNL
ALATSIEKQTPVRVIRGKHKSTHDKSKGGNYVYDGLYLVEKYWQQVGSHGMNVFKFQLRR
IPGQPELSWVEVKKSKSKYREGLCKLDISEGKEQSPISAVNEIDDEKPPLFTYTVKLIYP
DWCRPVPPKSCCCTTRCTEAEARVCACVEKNGGEIPYNFDGAIVGAKPTIYECGPLCKCP
SSCYLRVTQHGIKLPLEIFKTKSRGWGVRCLKSIPIGSFICEYVGELLEDSEAERRIGND
EYLFDIGNRYDNSLAQGMSELMLGTQAGRSMAEGDESSGFTIDAASKGNVGRFINHSCSP
NLYAQNVLYDHEDSRIPHVMFFAQDNIPPLQELCYDYNYALDQVRDSKGNIKQKPCFCGA
AVCRRRLY
>seq004
MSTLLPFPDLNLMPDSQSSTAGTTAGDTVVTGKLEVKSEPIEEWQTPPSSTSDQSANTDL
IAEFIRISELFRSAFKPLQVKGLDGVSVYGLDSGAIVAVPEKENRELIEPPPGFKDNRVS
TVVVSPKFERPRELARIAILGHEQRKELRQVMKRTRMTYESLRIHLMAESMKNHVLGQGR
RRRSDMAAAYIMRDRGLWLNYDKHIVGPVTGVEVGDIFFYRMELCVLGLHGQTQAGIDCL
TAERSATGEPIATSIVVSGGYEDDEDTGDVLVYTGHGGQDHQHKQCDNQRLVGGNLGMER
SMHYGIEVRVIRGIKYENSISSKVYVYDGLYKIVDWWFAVGKSGFGVFKFRLVRIEGQPM
MGSAVMRFAQTLRNKPSMVRPTGYVSFDLSNKKENVPVFLYNDVDGDQEPRHYEYIAKAV
FPPGIFGQGGISRTGCECKLSCTDDCLCARKNGGEFAYDDNGHLLKGKHVVFECGEFCTC
GPSCKSRVTQKGLRNRLEVFRSKETGWGVRTLDLIEAGAFICEYAGVVVTRLQAEILSMN
GDVMVYPGRFTDQWRNWGDLSQVYPDFVRPNYPSLPPLDFSMDVSRMRNVACYISHSKEP
NVMVQFVLHDHNHLMFPRVMLFALENISPLAELSLDYGLADEVNGKLAICN
>seq005
MVHSESSILSSLRGGDGGGIPCSKDELAINGSYTDPMGRRKSKRFKVAAESEFSPDFGSI
TRQLRSRRMQKEFTVETYETRNVSDVCVLSSQADVELIPGEIVAERDSFKSVDCNDMSVG
LTEGAESLGVNMQEPMKDRNMPENTSEQNMVEVHPPSISLPEEDMMGSVCRKSITGTKEL
HGRTISVGRDLSPNMGSKFSKNGKTAKRSISVEEENLVLEKSDSGDHLGPSPEVLELEKS
EVWIITDKGVVMPSPVKPSEKRNGDYGEGSMRKNSERVALDKKRLASKFRLSNGGLPSCS
SSGDSARYKVKETMRLFHETCKKIMQEEEARPRKRDGGNFKVVCEASKILKSKGKNLYSG
TQIIGTVPGVEVGDEFQYRMELNLLGIHRPSQSGIDYMKDDGGELVATSIVSSGGYNDVL
DNSDVLIYTGQGGNVGKKKNNEPPKDQQLVTGNLALKNSINKKNPVRVIRGIKNTTLQSS
VVAKNYVYDGLYLVEEYWEETGSHGKLVFKFKLRRIPGQPELPWKEVAKSKKSEFRDGLC
NVDITEGKETLPICAVNNLDDEKPPPFIYTAKMIYPDWCRPIPPKSCGCTNGCSKSKNCA
CIVKNGGKIPYYDGAIVEIKPLVYECGPHCKCPPSCNMRVSQHGIKIKLEIFKTESRGWG
VRSLESIPIGSFICEYAGELLEDKQAESLTGKDEYLFDLGDEDDPFTINAAQKGNIGRFI
NHSCSPNLYAQDVLYDHEEIRIPHIMFFALDNIPPLQELSYDYNYKIDQVYDSNGNIKKK
FCYCGSAECSGRLY
>seq006
MERNGGHYTDKTRVLDIKPLRTLRPVFPSGNQAPPFVCAPPFGPFPPGFSSFYPFSSSQA
NQHTPDLNQAQYPPQHQQPQNPPPVYQQQPPQHASEPSLVTPLRSFRSPDVSNGNAELEG
STVKRRIPKKRPISRPENMNFESGINVADRENGNRELVLSVLMRFDALRRRFAQLEDAKE
AVSGIIKRPDLKSGSTCMGRGVRTNTKKRPGIVPGVEIGDVFFFRFEMCLVGLHSPSMAG
IDYLVVKGETEEEPIATSIVSSGYYDNDEGNPDVLIYTGQGGNADKDKQSSDQKLERGNL
ALEKSLRRDSAVRVIRGLKEASHNAKIYIYDGLYEIKESWVEKGKSGHNTFKYKLVRAPG
QPPAFASWTAIQKWKTGVPSRQGLILPDMTSGVESIPVSLVNEVDTDNGPAYFTYSTTVK
YSESFKLMQPSFGCDCANLCKPGNLDCHCIRKNGGDFPYTGNGILVSRKPMIYECSPSCP
CSTCKNKVTQMGVKVRLEVFKTANRGWGLRSWDAIRAGSFICIYVGEAKDKSKVQQTMAN
DDYTFDTTNVYNPFKWNYEPGLADEDACEEMSEESEIPLPLIISAKNVGNVARFMNHSCS
PNVFWQPVSYENNSQLFVHVAFFAISHIPPMTELTYDYGVSRPSGTQNGNPLYGKRKCFC
GSAYCRGSFG
>seq007
MQGVPGFNTVPNPNHYDKSIVLDIKPLRSLKPVFPNGNQGPPFVGCPPFGPSSSEYSSFF
PFGAQQPTHDTPDLNQTQNTPIPSFVPPLRSYRTPTKTNGPSSSSGTKRGVGRPKGTTSV
KKKEKKTVANEPNLDVQVVKKFSSDFDSGISAAEREDGNAYLVSSVLMRFDAVRRRLSQV
EFTKSATSKAAGTLMSNGVRTNMKKRVGTVPGIEVGDIFFSRIEMCLVGLHMQTMAGIDY
IISKAGSDEESLATSIVSSGRYEGEAQDPESLIYSGQGGNADKNRQASDQKLERGNLALE
NSLRKGNGVRVVRGEEDAASKTGKIYIYDGLYSISESWVEKGKSGCNTFKYKLVRQPGQP
PAFGFWKSVQKWKEGLTTRPGLILPDLTSGAESKPVSLVNDVDEDKGPAYFTYTSSLKYS
ETFKLTQPVIGCSCSGSCSPGNHNCSCIRKNDGDLPYLNGVILVSRRPVIYECGPTCPCH
ASCKNRVIQTGLKSRLEVFKTRNRGWGLRSWDSLRAGSFICEYAGEVKDNGNLRGNQEED
AYVFDTSRVFNSFKWNYEPELVDEDPSTEVPEEFNLPSPLLISAKKFGNVARFMNHSCSP
NVFWQPVIREGNGESVIHIAFFAMRHIPPMAELTYDYGISPTSEARDESLLHGQRTCLCG
SEQCRGSFG
>seq008
MGSSHIPLDPSLNPSPSLIPKLEPVTESTQNLAFQLPNTNPQALISSAVSDFNEATDFSS
DYNTVAESARSAFAQRLQRHDDVAVLDSLTGAIVPVEENPEPEPNPYSTSDSSPSVATQR
PRPQPRSSELVRITDVGPESERQFREHVRKTRMIYDSLRMFLMMEEAKRNGVGGRRARAD
GKAGKAGSMMRDCMLWMNRDKRIVGSIPGVQVGDIFFFRFELCVMGLHGHPQSGIDFLTG
SLSSNGEPIATSVIVSGGYEDDDDQGDVIMYTGQGGQDRLGRQAEHQRLEGGNLAMERSM
YYGIEVRVIRGLKYENEVSSRVYVYDGLFRIVDSWFDVGKSGFGVFKYRLERIEGQAEMG
SSVLKFARTLKTNPLSVRPRGYINFDISNGKENVPVYLFNDIDSDQEPLYYEYLAQTSFP
PGLFVQQSGNASGCDCVNGCGSGCLCEAKNSGEIAYDYNGTLIRQKPLIHECGSACQCPP
SCRNRVTQKGLRNRLEVFRSLETGWGVRSLDVLHAGAFICEYAGVALTREQANILTMNGD
TLVYPARFSSARWEDWGDLSQVLADFERPSYPDIPPVDFAMDVSKMRNVACYISHSTDPN
VIVQFVLHDHNSLMFPRVMLFAAENIPPMTELSLDYGVVDDWNAKLAICN
>seq009
MDKSIPIKAIPVACVRPDLVDDVTKNTSTIPTMVSPVLTNMPSATSPLLMVPPLRTIWPS
NKEWYDGDAGPSSTGPIKREASDNTNDTAHNTFAPPPEMVIPLITIRPSDDSSNYSCDAG
AGPSTGPVKRGRGRPKGSKNSTPTEPKKPKVYDPNSLKVTSRGNFDSEITEAETETGNQE
IVDSVMMRFDAVRRRLCQINHPEDILTTASGNCTKMGVKTNTRRRIGAVPGIHVGDIFYY
WGEMCLVGLHKSNYGGIDFFTAAESAVEGHAAMCVVTAGQYDGETEGLDTLIYSGQGGTD
VYGNARDQEMKGGNLALEASVSKGNDVRVVRGVIHPHENNQKIYIYDGMYLVSKFWTVTG
KSGFKEFRFKLVRKPNQPPAYAIWKTVENLRNHDLIDSRQGFILEDLSFGAELLRVPLVN
EVDEDDKTIPEDFDYIPSQCHSGMMTHEFHFDRQSLGCQNCRHQPCMHQNCTCVQRNGDL
LPYHNNILVCRKPLIYECGGSCPCPDHCPTRLVQTGLKLHLEVFKTRNCGWGLRSWDPIR
AGTFICEFAGLRKTKEEVEEDDDYLFDTSKIYQRFRWNYEPELLLEDSWEQVSEFINLPT
QVLISAKEKGNVGRFMNHSCSPNVFWQPIEYENRGDVYLLIGLFAMKHIPPMTELTYDYG
VSCVERSEEDEGFLVCPYLSSSLWPSSSEIHFLINSKGRAWYDKIYRKLASQGNVSSGLD
SVKDEPEKLREEQMEGDGFKEKLSDSVLIDEKLEEYSDCDRTATTSRSHTDPVSSQSTHQ
TPESFRTPITCDDDTFVSVSGISRDVSNLIPFATETPASPVQEKMANTRSFSNNSVKGNQ
DEFFIEDFDVGPMDTIDLYDMTFREDPSDFDDNLLYAMRDRTKQLRSFKRKIMDAIKSKR
RREKEYEQLAIWFGDADMGCDLVNDKEQSTTSIDSKSSQTNVPVVSEDSEWEIL
>seq010
MMMTQRISPSNKRRRVSFVRDFPQFSVKDESDIGGDDVATIKENLDGKEDSNCVGVAYRD
HHRPKEESFDSIMKKAGFNVANGNLGNGKFPPSKRNVPLPCEGKVQPLSVEEGIKLMAYE
SQRRRCFGKPLVSTKVVQKHRYSPAKKKLSNATALRVRHSPMKKLSNASRLRANAHRPTQ
HKDERRSGVLSVIQRNRLSKDLTPRQKVQEVLRIFTLVFDELDRNKAARRGGSETAKSRI
DYQTWTILREMGMQVNSQKRIGSVPGIKVGDKIQFKAALSVIGLHFGIMSGIDYMYKGNK
EVATSIVSSEGNDYGDRFINDVMIYCGQGGNMRSKDHKAIKDQKLVGGNLALANSIKEKT
PVRVIRGERRLDNRGKDYVYDGLYRVEKYWEERGPQGNILFKFKLRRTCQPYVDF
>seq011
MSQKRSLVFAIRDFPPGCGTHIDVSSSLNHPAEKAFKHPRTGDVSGENLSFAEAKPEGTC
LKRESADQDHIFAAPEHNAKREPAGQDHVVAATTVAYATSSHRQKVEIGNSDCDPTPREK
VLEVLSLFKQVYNQLDRDKKARRGGDFLDATSRIDLKTLTVLEKMGKQVNTEKRIGSVPG
INIGDVFQYKTELRVVGLHSKPMCGIDYIKLGDDRITTSIVASEGYGYNDTYNSGVMVYT
GEGGNVINKQKKTEDQKLVKGNLALATSMRQKSQVRVIRGEERLDRKGKRYVYDGLYMVE
EYWVERDVRGKSVYKFKLCRIPGQLPLT
>seq012
MCLVGLHRNTAGGIDSLLAKESGVDGPAATSVVTSGKYDNETEDLETLIYSGHGGKPCDQ
VLQRGNRALEASVRRRNEVRVIRGELYNNEKVYIYDGLYLVSDCWQVTGKSGFKEYRFKL
LRKPGQPPGYAIWKLVENLRNHELIDPRQGFILGDLSFGEEGLRVPLVNEVDEEDKTIPD
DFDYIRSQCYSGMTNDVNVDSQSLVQSYIHQNCTCILKNCGQLPYHDNILVCRKPLIYEC
GGSCPTRMVETGLKLHLEVFKTSNCGWGLRSWDPIRAGTFICEFTGVSKTKEEVEEDDDY
LFDTSRIYHSFRWNYEPELLCEDACEQVSEDANLPTQVLISAKEKGNVGRFMNHNCWPNV
FWQPIEYDDNNGHIYVRIGLFAMKHIPPMTELTYDYGISCVEKTGEDEVIYKGKKICLCG
SVKCRGSFG
>seq013
MGLVGLHSGTIDMEFIGVEDHGDEEGKQIAVSVISSGKNADKTEDPDSLIFTGFGGTDMY
HGQPCNQKLERLNIPLEAAFRKKSIVRVVRCMKDEKRTNGNIYIYDGTYMITNRWEEEGQ
NGFIVFKFKLVREPDQKPAFGIWKSIQNWRNGLSIRPGLILEDLSNGAENLKVCLVNEVD
KENGPALFRYVTSLIHEVINNIPSMVDRCACGRRSCGSKHVFREKLSVSSSLVISAKKSG
NVARFMNHSCSPNVFWQSIAREQNGLWCLYIGFFAMKHIPPLTELRYDYGKSRGGGKKMC
LCRTKKCCGSFG
>seq014
MTRVNQLPCDCVSTAEESLTSGTCITPTHVTSLSSPLDRSGDVDPLPVSDESGGSKADES
MTDADETKKRKRILSGDCEADENNKSDGEIASLNDGVDAFTAICEDLNCSLCNQLPDRPV
TILCGHNFCLKCFDKWIDQGNQICATCRSTIPDKMAANPRVNSSLVSVIRYVKVAKTAGV
GTANFFPFTSNQDGPENAFRTKRAKIGEENAARIYVTVPFDHFGPIPAEHDPVRNQGVLV
GESWENRVECRQWGVHLPHVSCIAGQEDYGAQSVVISGGYKDDEDHGEWFLYTGRSRGRH
FANEDQEFEDLNEALRVSCEMGYPVRVVRSYKDRYSAYAPKEGVRYDGVYRIEKCWRKAR
FPVCRYLFVRCDNEPAPWNSDESGDRPRPLPNIPELETASDLFERKESPSWDFDEAEGRW
RWMKPPPANHEQRERMKMAMTCLLLFVLIILVGSSSILYQY
>seq015
MTRVNQLPCDCVSTAEESLTSGTCITPTHVTSLSSPLDRSGDVDPLPVSDESGGSKADES
MTDADETKKRKRILSGDCEADENNKSDGEIASLNDGVDAFTAICEDLNCSLCNQLPDRPV
TILCGHNFCLKCFDKWIDQGNQICATCRSTIPDKMAANPRVNSSLVSVIRYVKVAKTAGV
GTANFFPFTSNQDGPENAFRTKRAKIGEENAARIYVTVPFDHFGPIPAEHDPVRNQGVLV
GESWENRVECRQWGVHLPHVSCIAGQEDYGAQSVVISGGYKDDEDHGEWFLYTGRRSYKD
RYSAYAPKEGVRYDGVYRIEKCWRKARFPDSFKVCRYLFVRCDNEPAPWNSDESGDRPRP
LPNIPELETASDLFERKESPSWDFDEAEGRWRWMKPPPANHEQRERMKMAMTCLLLFVLI
ILVGSSSILYQY
>seq016
MAEQPRINSALVSVIRMAKVSKNANSAVSAAAYHYIRNDDRPDKAFTTERAKRAGKANAS
SGQIFVTIPPDHFGPILAENDPKRSIGVLVGDTWEDRLECRQWGAHFPHVAGIAGQSTHG
AQSVALSGGYVDDEDHGEWFLYTGSGGRDLSGNKRTNKEQSSDQKFEKLNAALRISCLKG
YPVRVVRSHKEKRSSYAPEAGVRYDGVYRIEKCWRKISVQGKFKVCRYLFVRCDNEPAPW
TSDIYGDRPRPLPKVDELKGATDISERKGTPSWDFDEKEGWKWVKPPPISRKPNLSGDPA
TDKEIRRVARRAQMSVTERLLKEFGCSICKQVMKEPLTTPCAHNFCKLCLVGTYGSQSSM
RERSRGGRTLRAQKIVKKCPSCPTDICDFLENPQINREMMDLIESLQRKAVEEGDTKTSS
DVSNGAESSGDDGNNEALEKGEDDSSLKDDGSLKDDGKVVKAVVVIKEEDLQPKKSKGED
EKEQGDKKMDSADVVDIAVEKKQATKRASEKAEKKQARKRKGDAVATNDGKRMKTGGDAM
ETAAEEDAPLSGGTPVKRNSRKSSEVDAKGGGGSPVVSSPRRVTRSNAKASGEADGSPAT
RTRRATRAEA
>seq017
MTPATQYPCDPEGVCMRCKSMPPPEESLTCGTCVTPWHVSCLLSPPETLSATLQWLCPDC
SGETNPLPVSGVAAGYGSVGSDLVAAIHSIEADETLSAEEKAKKKQQLLSGKGVVDEDDE
EEKKKTSKGKKPIDVLSHFECSFCMQSLQKPVSVRVLFALALMLVWFLESTPCGHNACLK
CFLKWMGQGHRSCGTCRSVIPESMVTNPRINLSIVSAIRLARVSEKADARTSKVVHYVDN
EDRPDKAFTTERAKKTGNANASSGKIFVTIPRDHFGPIPAENDPVRNQGLLVGESWKGRL
ACRQWGAHFPHVSGIAGQASYGAQSVVLAGGYDDDEDHGEWFLYTGRTNTVQAFDQVFLN
FNEALRLSCKLGYPVRVVRSTKDKRSPYAPQGGLLRYDGVYRIEKCWRIVGIQMCRFLFV
RCDNEPAPWTSDEHGDRPRPLPNVPELNMATDLFERKESPSWDFDEGEDRWRWMKPPPAS
KKAVKNVLDPEERKLLREAIKSANPNTMRARLLKEFKCQICQKVMTNPVTTPCAHNFCKA
CLESKFAGTALVRERGSGGRKLRSQKSVMKCPCCPTDIAEFVQNPQVNREVAEVIEKLKK
QEEEENAKSLDEGQCSGTSHEEEDDEQPKKRIKLDTDAEVSATVVESDMK
>seq018
MARDIQLPCDGDGVCMRCKSNPPPEESLTCGTCVTPWHVSCLSSPPKTLASTLQWHCPDC
SGEIDPLPVSGGATGFESAGSDLVAAIRAIEADESLSTEEKAKMRQRLLSGKGVEEDDEE
EKRKKKGKGKNPNLDVLSALGDNLMCSFCMQLPERPVTKPCGHNACLKCFEKWMGQGKRT
CGKCRSIIPEKMAKNPRINSSLVAAIRLAKVSKSAAATTSKVFHFISNQDRPDKAFTTER
AKKTGKANAASGKIYVTIPPDHFGPIPAENDPVRNQGLLVGESWEDRLECRQWGAHFPHV
AGIAGQSTYGAQSVALSGGYKDDEDHGEWFLYTGSGGRDLSGNKRTNKEQSFDQKFEKSN
AALKLSCKLGYPVRVVRSHKEKRSAYAPEEGVRYDGVYRIEKCWRKVGVQVCRYLFVRCD
NEPAPWTSDENGDRPRPIPNIPELNMATDLFERKETPSWDFDEGEGCWKWMKPPPASKKS
VNVLAPEERKNLRKAIKAAHSNTMRARLLKEFKCQICQQVLTLPVTTPCAHNFCKACLEA
KFAGKTLVRERSTGGRTLRSRKNVLNCPCCPTDISDFLQNPQVNREVAEVIEKLKTQEED
TAELEDEDEGECSGTTPEEDSEQPKKRIKLDTDATVSATIR
>seq019
MAIQTQLPCDGDGVCMRCQVTPPSEETLTCGTCVTPWHVSCLLPESLASSTGDWECPDCS
GVVVPSAAPGTGISGPESSGSVLVAAIRAIQADVTLTEAEKAKKRQRLMSGGGDDGVDDE
EKKKLEIFCSICIQLPERPVTTPCGHNFCLKCFEKWAVGQGKLTCMICRSKIPRHVAKNP
RINLALVSAIRLANVTKCSGEATAAKVHHIIRNQDRPDKAFTTERAVKTGKANAASGKFF
VTIPRDHFGPIPAANDVTRNQGVLVGESWEDRQECRQWGVHFPHVAGIAGQAAVGAQSVA
LSGGYDDDEDHGEWFLYTGSGGRDLSGNKRVNKIQSSDQAFKNMNEALRLSCKMGYPVRV
VRSWKEKRSAYAPAEGVRYDGVYRIEKCWSNVGVQGLHKMCRYLFVRCDNEPAPWTSDEH
GDRPRPLPDVPELENATDLFVRKESPSWGFDEAEGRWKWMKSPPVSRMALDTEERKKNKR
AKKGNNAMKARLLKEFSCQICRKVLSLPVTTPCAHNFCKACLEAKFAGITQLRDRSNGVR
KLRAKKNIMTCPCCTTDLSEFLQNPQVNREMMEIIENFKKSEEEAEVAESSNISEEEEEE
SEPPTKKIKMDNNSVGDTSLSA
>seq020
MAIQTQLPCDGDGVCMRCQVNPPSEETLTCGTCVTPWHVSCLLPESLASSTGDWECPDCS
GVVVPSAAPGTGISGPESSGSVLVTAIRAIQADVTLTEAEKAKKRQRLMSGGGDDGVDDE
EKKKLEIFCSICIQLPERPVTTPCGHNFCLKCFEKWAVGQGKLTCMICRSKIPRHVAKNP
RINLALVSAIRLANVTKCSGEATAAKVHHIIRNQDRPDKAFTTERAVKTGKANAASGVLV
GESWEDRQECRQWGVHFPHVAGIAGQAAVGAQSVALSGGYDDDEDHGEWFLYTGSGGRDL
SGNKRVNKIQSSDQAFKNMNEALRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDGVYRI
EKCWSNVGVQGLHKMCRYLFVRCDNEPAPWTSDEHGDRPRPLPDVPELENATDLFVRKES
PSWGFDEAEGRWKWMKSPPVSRMALDTEERKKNKRAKKGNNAMKARLLKEFSCQICRKVL
SLPVTTPCAHNFCKACLEAKFAGITQLRDRSNGVRKLRAKKNIMTCPCCTTDLSEFLQNP
QVNREMMEIIENFKKSEEEAEVAESSNISEEEGEEESEPPTKKIKMDKNSVGGTSLSA
>seq021
MAIETQLPCDGDGVCMRCQVNPPSEETLTCGTCVTPWHVPCLLPESLASSTGEWECPDCS
GVVVPSAAPGTGNARPESSGSVLVAAIRAIQADETLTEAEKAKKRQKLMSGGGDDGVDEE
EKKKLEIFCSICIQLPERPITTPCGHNFCLKCFEKWAVGQGKLTCMICRSKIPRHVAKNP
RINLALVSAIRLANVTKCSVEATAAKVHHIIRNQDRPEKAFTTERAVKTGKANAASGKFF
VTIPRDHFGPIPAENDVTRKQGVLVGESWEDRQECRQWGAHFPHIAGIAGQSAVGAQSVA
LSGGYDDDEDHGEWFLYTGSGGRDLSGNKRINKKQSSDQAFKNMNESLRLSCKMGYPVRV
VRSWKEKRSAYAPAEGVRYDGVYRIEKCWSNVGVQGSFKVCRYLFVRCDNEPAPWTSDEH
GDRPRPLPNVPELETAADLFVRKESPSWDFDEAEGRWKWMKSPPVSRMALDPEERKKNKR
AKNTMKARLLKEFSCQICREVLSLPVTTPCAHNFCKACLEAKFAGITQLRERSNGGRKLR
AKKNIMTCPCCTTDLSEFLQNPQVNREMMEIIENFKKSEEEADASISEEEEEESEPPTKK
IKMDNNSVGGSGTSLSA
>seq022
MWIQVRTMDGRQTHTVDSLSRLTKVEELRRKIQELFHVEPGLQRLFYRGKQMEDGHTLFD
YEVRLNDTIQLLVRQSLVLPHSTKERDSELSDTDSGCCLGQSESDKSSTHGEAAAETDSR
PADEDMWDETELGLYKVNEYVDARDTNMGAWFEAQVVRVTRKAPSRDEPCSSTSRPALEE
DVIYHVKYDDYPENGVVQMNSRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKERGFWYD
AEISRKRETRTARELYANVVLGDDSLNDCRIIFVDEVFKIERPGEGSPMVDNPMRRKSGP
SCKHCKDDVNRLCRVCACHLCGGRQDPDKQLMCDECDMAFHIYCLDPPLSSVPSEDEWYC
PECRNDASEVVLAGERLRESKKNAKMASATSSSQRDWGKGMACVGRTKECTIVPSNHYGP
IPGIPVGTMWRFRVQVSESGVHRPHVAGIHGRSNDGSYSLVLAGGYEDDVDHGNFFTYTG
SGGRDLSGNKRTAEQSCDQKLTNTNRALALNCFAPINDQEGAEAKDWRSGKPVRVVRNVK
GGKNSKYAPAEGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDDEPGPWTKEGKDRIKK
LGLTMQYPEGYLEALANREREKENSKREEEEQQEGGFASPRTGKGKWKRKSAGGGPSRAG
SPRRTSKKTKVEPYSLTAQQSSLIREDKSNAKLWNEVLASLKDRPASGSPFQLFLSKVEE
TFQCICCQELVFRPITTVCQHNVCKDCLDRSFRAQVFSCPACRYDLGRSYAMQVNQPLQT
VLNQLFPGYGNGR
>seq023
MWIQVRTMDGKETHTVNSLSRLTKVQELRKKIEEVFHVEPQLQRLFYRGKQMEDGHTLFD
YDVRLNDTIQLLVRQSLALPLSTKERDSELSDSDSGYGVGHSESDKSSTHGEGAAEADDK
TVWEDTDLGLYKVNEYVDVRDNIFGAWFEAQVVQVQKRALSEDEPCSSSAVKTSEDDIMY
HVKYDDYPEHGVDIVKAKNVRARARTVIPWENLEVGQVVMANYNVDYPRKRGFWYDVEIC
RKRQTRTARELYGNIRLLNDSQLNNCRIMFVDEVLMIELPKERRPLIASPSQPPPALRNT
GKSGPSCRFCKDDENKPCRKCACHVCGGREAPEKQLLCDECDMAFHLYCLKPPLTSVPPE
PEWYCPSCRTDSSEVVQAGEKLKESKKKAKMASATSSSRRDWGKGMACVGRTTECTIVPA
NHFGPIPGVPVGTMWRFRVQVSESGVHRPHVAGIHGRSNDGAYSLVLAGGYEDDVDNGNY
FTYTGSGGRDLSGNKRTAGQSSDQKLTNNNRALALNCHSPINEKGAEAEDWRQGKPVRVV
RNMKGGKHSKYAPAEGNRYDGIYKVVKYWPERGKSGFLVWRYLLRRDDTEPEPWTREGKD
RTRQLGLTMQYPEGYLEALANKEKSRKRPAKALEQGPSSSKTGKSKQKSTGPTLSSPRAS
KKSKLEPYTLSEQQANLIKEDKGNAKLWDDVLTSLQDGPYQIFLSKVKEAFQCICCQELV
FRPVTTVCQHNVCKDCLDRSFRAQVFSCPACRFELDHSSPTRVNQPLQTILNQLFPGYGS
GR

Binary file not shown.

Binary file not shown.

Binary file not shown.

Binary file not shown.

View file

@ -0,0 +1,60 @@
# E. coli paired-end test dataset for Bisulfite-seq applications
The E. coli data set was generated using [Sherman](https://github.com/FelixKrueger/Sherman) as 10,000 reads of paired-end data, with average methylation levels of 80% in CpG context, and 10% in non-CG context. The files can be found in the folder: genaral/fastq/dna, and are called:
`Ecoli_10K_methylated_R1.fastq.gz`
`Ecoli_10K_methylated_R2.fastq.gz`
```bash
Sherman --non_dir --genome /bi/scratch/Genomes/E_coli/ --paired -n 10000 -l 100 --CG 20 --CH 90
```
The data is non-directional, so it should produce roughly 25% mapping to each of the `OT`, `CTOT`, `CTOB` and `OB` strands. Thus, the data can be used for bisulfite mapping in standard (= directional), `--pbat` and `--non_directional` mode.
A test alignment should look roughly like this:
`bismark --genome /bi/scratch/Genomes/E_coli/ -1 Ecoli_10K_methylated_R1.fastq.gz -2 Ecoli_10K_methylated_R2.fastq.gz --non_dir`
``` csv
Bismark report for: Ecoli_10K_methylated_R1.fastq.gz and Ecoli_10K_methylated_R2.fastq.gz (version: v0.22.3)
Bismark was run with Bowtie 2 against the bisulfite genome of /bi/scratch/Genomes/E_coli/ with the specified options: -q --score-min L,0,-0.2 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500
Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB)
Final Alignment report
======================
Sequence pairs analysed in total: 10000
Number of paired-end alignments with a unique best hit: 9320
Mapping efficiency: 93.2%
Sequence pairs with no alignments under any condition: 0
Sequence pairs did not map uniquely: 680
Sequence pairs which were discarded because genomic sequence could not be extracted: 0
Number of sequence pairs with unique best (first) alignment came from the bowtie output:
CT/GA/CT: 2341 ((converted) top strand)
GA/CT/CT: 2329 (complementary to (converted) top strand)
GA/CT/GA: 2356 (complementary to (converted) bottom strand)
CT/GA/GA: 2294 ((converted) bottom strand)
Final Cytosine Methylation Report
=================================
Total number of C's analysed: 471997
Total methylated C's in CpG context: 111011
Total methylated C's in CHG context: 11923
Total methylated C's in CHH context: 21433
Total methylated C's in Unknown context: 0
Total unmethylated C's in CpG context: 27790
Total unmethylated C's in CHG context: 105877
Total unmethylated C's in CHH context: 193963
Total unmethylated C's in Unknown context: 0
C methylated in CpG context: 80.0%
C methylated in CHG context: 10.1%
C methylated in CHH context: 10.0%
Can't determine percentage of methylated Cs in unknown context (CN or CHN) if value was 0
Bismark completed in 0d 0h 0m 12s
```

View file

@ -0,0 +1,7 @@
# Paired-end RNA-seq test dataset
The data here are 2x 76bp RNA-seq data from mouse (10,000 reads of paired-end data). The files can be found in the folder: genaral/fastq/rna, and are called:
`test_R1.fastq.gz`
`test_R2.fastq.gz`

Binary file not shown.

Binary file not shown.