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40
modules/kaiju/kaiju2table/main.nf
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40
modules/kaiju/kaiju2table/main.nf
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process KAIJU_KAIJU2TABLE {
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tag "$meta.id"
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label 'process_low'
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conda (params.enable_conda ? "bioconda::kaiju=1.8.2" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/kaiju:1.8.2--h5b5514e_1':
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'quay.io/biocontainers/kaiju:1.8.2--h2e03b76_0' }"
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input:
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tuple val(meta), path(results)
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path db
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val taxon_rank
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output:
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tuple val(meta), path('*.txt'), emit: summary
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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"""
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dbnodes=`find -L ${db} -name "*nodes.dmp"`
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dbname=`find -L ${db} -name "*.fmi" -not -name "._*"`
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kaiju2table $args \\
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-t \$dbnodes \\
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-n \$dbname \\
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-r ${taxon_rank} \\
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-o ${prefix}.txt \\
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${results}
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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kaiju: \$(echo \$( kaiju -h 2>&1 | sed -n 1p | sed 's/^.*Kaiju //' ))
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END_VERSIONS
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"""
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}
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50
modules/kaiju/kaiju2table/meta.yml
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modules/kaiju/kaiju2table/meta.yml
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name: "kaiju_kaiju2table"
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description: write your description here
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keywords:
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- classify
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- metagenomics
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tools:
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- kaiju:
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description: Fast and sensitive taxonomic classification for metagenomics
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homepage: https://kaiju.binf.ku.dk/
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documentation: https://github.com/bioinformatics-centre/kaiju/blob/master/README.md
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tool_dev_url: https://github.com/bioinformatics-centre/kaiju
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doi: "10.1038/ncomms11257"
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licence: ["GNU GPL v3"]
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input:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- results:
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type: file
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description: File containing the kaiju classification results
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pattern: "*.{txt}"
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- taxon_rank:
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type: string
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description: |
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Taxonomic rank to display in report
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pattern: "phylum|class|order|family|genus|species"
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output:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- versions:
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type: file
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description: File containing software versions
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pattern: "versions.yml"
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- results:
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type: file
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description: |
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Summary table for a given taxonomic rank
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pattern: "*.{tsv}"
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authors:
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- "@sofstam"
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- "@talnor"
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- "@jfy133"
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@ -1009,6 +1009,10 @@ kaiju/kaiju:
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- modules/kaiju/kaiju/**
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- tests/modules/kaiju/kaiju/**
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kaiju/kaiju2table:
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- modules/kaiju/kaiju2table/**
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- tests/modules/kaiju/kaiju2table/**
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kallisto/index:
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- modules/kallisto/index/**
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- tests/modules/kallisto/index/**
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21
tests/modules/kaiju/kaiju2table/main.nf
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21
tests/modules/kaiju/kaiju2table/main.nf
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#!/usr/bin/env nextflow
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nextflow.enable.dsl = 2
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include { UNTAR } from '../../../../modules/untar/main.nf'
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include { KAIJU_KAIJU } from '../../../../modules/kaiju/kaiju/main.nf'
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include { KAIJU_KAIJU2TABLE } from '../../../../modules/kaiju/kaiju2table/main.nf'
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workflow test_kaiju_kaiju_single_end {
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input = [
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[ id:'test', single_end:true ], // meta map
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file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
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]
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db = [ [], file(params.test_data['sarscov2']['genome']['kaiju_tar_gz'], checkIfExists: true) ]
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taxon_rank = "species"
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ch_db = UNTAR ( db )
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KAIJU_KAIJU ( input, ch_db.untar.map{ it[1] } )
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KAIJU_KAIJU2TABLE ( KAIJU_KAIJU.out.results, ch_db.untar.map{ it[1] }, taxon_rank )
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}
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5
tests/modules/kaiju/kaiju2table/nextflow.config
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5
tests/modules/kaiju/kaiju2table/nextflow.config
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process {
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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}
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9
tests/modules/kaiju/kaiju2table/test.yml
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9
tests/modules/kaiju/kaiju2table/test.yml
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- name: kaiju kaiju2table test_kaiju_kaiju_single_end
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command: nextflow run tests/modules/kaiju/kaiju2table -entry test_kaiju_kaiju_single_end -c tests/config/nextflow.config
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tags:
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- kaiju
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- kaiju/kaiju2table
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files:
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- path: output/kaiju/test.txt
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md5sum: 0d9f8fd36fcf2888296ae12632c5f0a8
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- path: output/kaiju/versions.yml
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