diff --git a/tests/software/fastqc/test.yml b/tests/software/fastqc/test.yml
index 5b2ab54f..f0f6b420 100644
--- a/tests/software/fastqc/test.yml
+++ b/tests/software/fastqc/test.yml
@@ -4,7 +4,6 @@
     - fastqc
   files:
     - path: output/test_single_end/test_fastqc.html
-      md5sum: e6b300cebca25e208ac32aacc430392f
     - path: output/test_single_end/test_fastqc.zip
 
 - name: Run fastqc paired-end test workflow
@@ -13,8 +12,6 @@
     - fastqc
   files:
     - path: output/test_paired_end/test_1_fastqc.html
-      md5sum: ac93f43b49c9eb52c3c0fcdb74f603cf
     - path: output/test_paired_end/test_2_fastqc.html
-      md5sum: 67ef5d28c60f5e075e39d4e95b31340e
     - path: output/test_paired_end/test_1_fastqc.zip
     - path: output/test_paired_end/test_2_fastqc.zip