diff --git a/tests/modules/vsearch/usearchglobal/test.yml b/tests/modules/vsearch/usearchglobal/test.yml
index 79bcd50e..a593f78f 100644
--- a/tests/modules/vsearch/usearchglobal/test.yml
+++ b/tests/modules/vsearch/usearchglobal/test.yml
@@ -5,7 +5,16 @@
     - vsearch
   files:
     - path: output/vsearch/test.aln
-      md5sum: 2543c4147530dcb1ba4550d3fdb1502a
+      contains:
+      - "vsearch --usearch_global transcriptome.fasta --db genome.fasta --id 0.985 --threads 2 --alnout test.aln"
+      - "Query >lcl|MT192765.1_cds_QIK50427.1_2"
+      - "%Id   TLen  Target"
+      - "100%  29829  MT192765.1"
+      - "Query  3822nt >lcl|MT192765.1_cds_QIK50427.1_2"
+      - "Target 29829nt >MT192765.1"
+      - "Qry 21249 + CAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAA 21291"
+      - "Tgt 21506 + CAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAA 21548"
+      - "21291 cols, 21290 ids (100.0%), 1 gaps (0.0%)"
 
 - name: vsearch usearchglobal test_vsearch_usearchglobal_userout
   command: nextflow run ./tests/modules/vsearch/usearchglobal -entry test_vsearch_usearchglobal_userout -c ./tests/config/nextflow.config  -c ./tests/modules/vsearch/usearchglobal/nextflow.config