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Merge branch 'master' into gatk_426

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FriederikeHanssen 2022-05-12 19:33:07 +02:00 committed by GitHub
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38 changed files with 862 additions and 334 deletions
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32
modules/arriba/main.nf
View file

@ -2,15 +2,20 @@ process ARRIBA {
tag "$meta.id"
label 'process_medium'
conda (params.enable_conda ? "bioconda::arriba=2.1.0" : null)
conda (params.enable_conda ? "bioconda::arriba=2.2.1" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/arriba:2.1.0--h3198e80_1' :
'quay.io/biocontainers/arriba:2.1.0--h3198e80_1' }"
'https://depot.galaxyproject.org/singularity/arriba:2.2.1--hecb563c_2' :
'quay.io/biocontainers/arriba:2.2.1--hecb563c_2' }"
input:
tuple val(meta), path(bam)
path fasta
path gtf
path blacklist
path known_fusions
path structural_variants
path tags
path protein_domains
output:
tuple val(meta), path("*.fusions.tsv") , emit: fusions
@ -23,7 +28,12 @@ process ARRIBA {
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def blacklist = (args.contains('-b')) ? '' : '-f blacklist'
def blacklist = blacklist ? "-b $blacklist" : "-f blacklist"
def known_fusions = known_fusions ? "-k $known_fusions" : ""
def structural_variants = structural_variants ? "-d $structual_variants" : ""
def tags = tags ? "-t $tags" : ""
def protein_domains = protein_domains ? "-p $protein_domains" : ""
"""
arriba \\
-x $bam \\
@ -32,6 +42,10 @@ process ARRIBA {
-o ${prefix}.fusions.tsv \\
-O ${prefix}.fusions.discarded.tsv \\
$blacklist \\
$known_fusions \\
$structural_variants \\
$tags \\
$protein_domains \\
$args
cat <<-END_VERSIONS > versions.yml
@ -39,4 +53,14 @@ process ARRIBA {
arriba: \$(arriba -h | grep 'Version:' 2>&1 | sed 's/Version:\s//')
END_VERSIONS
"""
stub:
def prefix = task.ext.prefix ?: "${meta.id}"
"""
echo stub > ${prefix}.fusions.tsv
echo stub > ${prefix}.fusions.discarded.tsv
echo "${task.process}:" > versions.yml
echo ' arriba: 2.2.1' >> versions.yml
"""
}

22
modules/arriba/meta.yml
View file

@ -30,6 +30,26 @@ input:
type: file
description: Annotation GTF file
pattern: "*.{gtf}"
- blacklist:
type: file
description: Blacklist file
pattern: "*.{tsv}"
- known_fusions:
type: file
description: Known fusions file
pattern: "*.{tsv}"
- structural_variants:
type: file
description: Structural variants file
pattern: "*.{tsv}"
- tags:
type: file
description: Tags file
pattern: "*.{tsv}"
- protein_domains:
type: file
description: Protein domains file
pattern: "*.{gff3}"
output:
- meta:
@ -51,4 +71,4 @@ output:
pattern: "*.{fusions.discarded.tsv}"
authors:
- "@praveenraj2018"
- "@praveenraj2018,@rannick"

69
modules/bowtie2/align/main.nf
View file

@ -1,11 +1,11 @@
process BOWTIE2_ALIGN {
tag "$meta.id"
label 'process_high'
label "process_high"
conda (params.enable_conda ? 'bioconda::bowtie2=2.4.4 bioconda::samtools=1.15.1 conda-forge::pigz=2.6' : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:1744f68fe955578c63054b55309e05b41c37a80d-0' :
'quay.io/biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:1744f68fe955578c63054b55309e05b41c37a80d-0' }"
conda (params.enable_conda ? "bioconda::bowtie2=2.4.4 bioconda::samtools=1.15.1 conda-forge::pigz=2.6" : null)
container "${ workflow.containerEngine == "singularity" && !task.ext.singularity_pull_docker_container ?
"https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:1744f68fe955578c63054b55309e05b41c37a80d-0" :
"quay.io/biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:1744f68fe955578c63054b55309e05b41c37a80d-0" }"
input:
tuple val(meta), path(reads)
@ -13,59 +13,50 @@ process BOWTIE2_ALIGN {
val save_unaligned
output:
tuple val(meta), path('*.bam') , emit: bam
tuple val(meta), path('*.log') , emit: log
tuple val(meta), path('*fastq.gz'), emit: fastq, optional:true
tuple val(meta), path("*.bam") , emit: bam
tuple val(meta), path("*.log") , emit: log
tuple val(meta), path("*fastq.gz"), emit: fastq, optional:true
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: ''
def args = task.ext.args ?: ""
def args2 = task.ext.args2 ?: ""
def prefix = task.ext.prefix ?: "${meta.id}"
if (meta.single_end) {
def unaligned = save_unaligned ? "--un-gz ${prefix}.unmapped.fastq.gz" : ''
"""
INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'`
[ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed 's/.rev.1.bt2l//'`
[ -z "\$INDEX" ] && echo "BT2 index files not found" 1>&2 && exit 1
bowtie2 \\
-x \$INDEX \\
-U $reads \\
--threads $task.cpus \\
$unaligned \\
$args \\
2> ${prefix}.bowtie2.log \\
| samtools view -@ $task.cpus $args2 -bhS -o ${prefix}.bam -
cat <<-END_VERSIONS > versions.yml
"${task.process}":
bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//')
samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
END_VERSIONS
"""
def unaligned = ""
def reads_args = ""
if (meta.single_end) {
unaligned = save_unaligned ? "--un-gz ${prefix}.unmapped.fastq.gz" : ""
reads_args = "-U ${reads}"
} else {
def unaligned = save_unaligned ? "--un-conc-gz ${prefix}.unmapped.fastq.gz" : ''
unaligned = save_unaligned ? "--un-conc-gz ${prefix}.unmapped.fastq.gz" : ""
reads_args = "-1 ${reads[0]} -2 ${reads[1]}"
}
def samtools_command = "samtools view -@ $task.cpus --bam --with-header ${args2} > ${prefix}.bam"
"""
INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'`
[ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed 's/.rev.1.bt2l//'`
[ -z "\$INDEX" ] && echo "BT2 index files not found" 1>&2 && exit 1
INDEX=`find -L ./ -name "*.rev.1.bt2" | sed "s/.rev.1.bt2//"`
[ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed "s/.rev.1.bt2l//"`
[ -z "\$INDEX" ] && echo "Bowtie2 index files not found" 1>&2 && exit 1
bowtie2 \\
-x \$INDEX \\
-1 ${reads[0]} \\
-2 ${reads[1]} \\
$reads_args \\
--threads $task.cpus \\
$unaligned \\
$args \\
2> ${prefix}.bowtie2.log \\
| samtools view -@ $task.cpus $args2 -bhS -o ${prefix}.bam -
| $samtools_command
if [ -f ${prefix}.unmapped.fastq.1.gz ]; then
mv ${prefix}.unmapped.fastq.1.gz ${prefix}.unmapped_1.fastq.gz
fi
if [ -f ${prefix}.unmapped.fastq.2.gz ]; then
mv ${prefix}.unmapped.fastq.2.gz ${prefix}.unmapped_2.fastq.gz
fi
@ -77,5 +68,5 @@ process BOWTIE2_ALIGN {
pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
END_VERSIONS
"""
}
}

20
modules/custom/sratoolsncbisettings/main.nf Normal file
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@ -0,0 +1,20 @@
process CUSTOM_SRATOOLSNCBISETTINGS {
tag 'ncbi-settings'
label 'process_low'
conda (params.enable_conda ? 'bioconda::sra-tools=2.11.0' : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/sra-tools:2.11.0--pl5321ha49a11a_3' :
'quay.io/biocontainers/sra-tools:2.11.0--pl5321ha49a11a_3' }"
output:
path('*.mkfg') , emit: ncbi_settings
path 'versions.yml', emit: versions
when:
task.ext.when == null || task.ext.when
shell:
config = "/LIBS/GUID = \"${UUID.randomUUID().toString()}\"\\n/libs/cloud/report_instance_identity = \"true\"\\n"
template 'detect_ncbi_settings.sh'
}

28
modules/custom/sratoolsncbisettings/meta.yml Normal file
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@ -0,0 +1,28 @@
name: "sratoolsncbisettings"
description: Test for the presence of suitable NCBI settings or create them on the fly.
keywords:
- NCBI
- settings
- sra-tools
- prefetch
- fasterq-dump
tools:
- "sratools":
description: "SRA Toolkit and SDK from NCBI"
homepage: https://github.com/ncbi/sra-tools
documentation: https://github.com/ncbi/sra-tools/wiki
tool_dev_url: https://github.com/ncbi/sra-tools
licence: "['Public Domain']"
output:
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
- ncbi_settings:
type: file
description: An NCBI user settings file.
pattern: "*.mkfg"
authors:
- "@Midnighter"

45
modules/custom/sratoolsncbisettings/templates/detect_ncbi_settings.sh Normal file
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@ -0,0 +1,45 @@
#!/usr/bin/env bash
set -u
# Get the expected NCBI settings path and define the environment variable
# `NCBI_SETTINGS`.
eval "$(vdb-config -o n NCBI_SETTINGS | sed 's/[" ]//g')"
# If the user settings do not exist yet, create a file suitable for `prefetch`
# and `fasterq-dump`. If an existing settings file does not contain the required
# values, error out with a helpful message.
if [[ ! -f "${NCBI_SETTINGS}" ]]; then
printf '!{config}' > 'user-settings.mkfg'
else
prefetch --help &> /dev/null
if [[ $? = 78 ]]; then
echo "You have an existing vdb-config at '${NCBI_SETTINGS}' but it is"\
"missing the required entries for /LIBS/GUID and"\
"/libs/cloud/report_instance_identity."\
"Feel free to add the following to your settings file:" >&2
echo "$(printf '!{config}')" >&2
exit 1
fi
fasterq-dump --help &> /dev/null
if [[ $? = 78 ]]; then
echo "You have an existing vdb-config at '${NCBI_SETTINGS}' but it is"\
"missing the required entries for /LIBS/GUID and"\
"/libs/cloud/report_instance_identity."\
"Feel free to add the following to your settings file:" >&2
echo "$(printf '!{config}')" >&2
exit 1
fi
if [[ "${NCBI_SETTINGS}" != *.mkfg ]]; then
echo "The detected settings '${NCBI_SETTINGS}' do not have the required"\
"file extension '.mkfg'." >&2
exit 1
fi
cp "${NCBI_SETTINGS}" ./
fi
cat <<-END_VERSIONS > versions.yml
"!{task.process}":
sratools: $(vdb-config --version 2>&1 | grep -Eo '[0-9.]+')
END_VERSIONS

59
modules/rtgtools/vcfeval/main.nf Normal file
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@ -0,0 +1,59 @@
process RTGTOOLS_VCFEVAL {
tag "$meta.id"
label 'process_medium'
conda (params.enable_conda ? "bioconda::rtg-tools=3.12.1" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/rtg-tools:3.12.1--hdfd78af_0':
'quay.io/biocontainers/rtg-tools:3.12.1--hdfd78af_0' }"
input:
tuple val(meta), path(query_vcf), path(query_vcf_tbi)
tuple path(truth_vcf), path(truth_vcf_tbi)
path(truth_regions)
path(evaluation_regions)
path(sdf)
output:
tuple val(meta), path("**results/{done,progress,*.log}") , emit: logs
tuple val(meta), path("**tp.vcf.gz"), path("**tp.vcf.gz.tbi") , emit: tp
tuple val(meta), path("**fn.vcf.gz"), path("**fn.vcf.gz.tbi") , emit: fn
tuple val(meta), path("**fp.vcf.gz"), path("**fp.vcf.gz.tbi") , emit: fp
tuple val(meta), path("**baseline.vcf.gz"), path("**baseline.vcf.gz.tbi") , emit: baseline
tuple val(meta), path("**.tsv.gz") , emit: roc
tuple val(meta), path("**results/summary.txt") , emit: summary
tuple val(meta), path("**results/phasing.txt") , emit: phasing
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ""
def prefix = task.ext.prefix ?: "${meta.id}"
def bed_regions = truth_regions ? "--bed-regions=$truth_regions" : ""
def eval_regions = evaluation_regions ? "--evaluation-regions=$evaluation_regions" : ""
def truth_index = truth_vcf_tbi ? "" : "rtg index $truth_vcf"
def query_index = query_vcf_tbi ? "" : "rtg index $query_vcf"
"""
$truth_index
$query_index
rtg vcfeval \\
$args \\
--baseline=$truth_vcf \\
$bed_regions \\
$eval_regions \\
--calls=$query_vcf \\
--output=${prefix}_results \\
--template=$sdf \\
--threads=$task.cpus \\
cat <<-END_VERSIONS > versions.yml
"${task.process}":
rtg-tools: \$(echo \$(rtg version | head -n 1 | awk '{print \$4}'))
END_VERSIONS
"""
}

95
modules/rtgtools/vcfeval/meta.yml Normal file
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@ -0,0 +1,95 @@
name: "rtgtools_vcfeval"
description: The VCFeval tool of RTG tools. It is used to evaluate called variants for agreement with a baseline variant set
keywords:
- benchmarking
- vcf
- rtg-tools
tools:
- "rtgtools":
description: "RealTimeGenomics Tools -- Utilities for accurate VCF comparison and manipulation"
homepage: "https://www.realtimegenomics.com/products/rtg-tools"
documentation: "https://github.com/RealTimeGenomics/rtg-tools"
tool_dev_url: "https://github.com/RealTimeGenomics/rtg-tools"
doi: ""
licence: "['BSD']"
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- truth_vcf:
type: file
description: A standard VCF to compare against
pattern: "*.{vcf,vcf.gz}"
- truth_vcf_index:
type: file
description: The index of the standard VCF (optional)
pattern: "*.tbi"
- query_vcf:
type: file
description: A VCF with called variants to benchmark against the standard
pattern: "*.{vcf,vcf.gz}"
- query_vcf_index:
type: file
description: The index of the called VCF (optional)
pattern: "*.tbi"
- truth_regions:
type: file
description: A BED file containining the strict regions where VCFeval should only evaluate the fully overlapping variants (optional)
pattern: "*.bed"
- evaluation_regions:
type: file
description: A BED file containing the regions where VCFeval will evaluate every fully and partially overlapping variant (optional)
pattern: "*.bed"
- sdf:
type: file
description: The SDF (RTG Sequence Data File) folder of the reference genome
pattern: "*"
output:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
- logging:
type: file
description: Files containing logging from vcfeval
pattern: "*{done,progress,.log}"
- tp:
type: file
description: A tuple containing the VCF and TBI file for the true positive variants
pattern: "tp.vcf{.gz,.gz.tbi}"
- baseline:
type: file
description: A tuple containing the VCF and TBI file for the baseline true positive variants
pattern: "tp-baseline.vcf{.gz,.gz.tbi}"
- fp:
type: file
description: A tuple containing the VCF and TBI file for the false positive variants
pattern: "fp.vcf{.gz,.gz.tbi}"
- fn:
type: file
description: A tuple containing the VCF and TBI file for the false negative variants
pattern: "fn.vcf{.gz,.gz.tbi}"
- roc:
type: file
description: TSV files containing ROC data for the evaluated variants
pattern: "*.tsv.gz"
- summary:
type: file
description: A TXT file containing the summary of the evaluation
pattern: "summary.txt"
- phasing:
type: file
description: A TXT file containing the data on the phasing
pattern: "phasing.txt"
authors:
- "@nvnieuwk"

8
modules/sratools/fasterqdump/main.nf
View file

@ -9,6 +9,7 @@ process SRATOOLS_FASTERQDUMP {
input:
tuple val(meta), path(sra)
path ncbi_settings
output:
tuple val(meta), path(output), emit: reads
@ -20,17 +21,12 @@ process SRATOOLS_FASTERQDUMP {
script:
def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: ''
def config = "/LIBS/GUID = \"${UUID.randomUUID().toString()}\"\\n/libs/cloud/report_instance_identity = \"true\"\\n"
// Paired-end data extracted by fasterq-dump (--split-3 the default) always creates
// *_1.fastq *_2.fastq files but sometimes also an additional *.fastq file
// for unpaired reads which we ignore here.
output = meta.single_end ? '*.fastq.gz' : '*_{1,2}.fastq.gz'
"""
eval "\$(vdb-config -o n NCBI_SETTINGS | sed 's/[" ]//g')"
if [[ ! -f "\${NCBI_SETTINGS}" ]]; then
mkdir -p "\$(dirname "\${NCBI_SETTINGS}")"
printf '${config}' > "\${NCBI_SETTINGS}"
fi
export NCBI_SETTINGS="\$PWD/${ncbi_settings}"
fasterq-dump \\
$args \\

7
modules/sratools/fasterqdump/meta.yml
View file

@ -10,7 +10,7 @@ tools:
homepage: https://github.com/ncbi/sra-tools
documentation: https://github.com/ncbi/sra-tools/wiki
tool_dev_url: https://github.com/ncbi/sra-tools
licence: ["US-Government-Work"]
licence: ["Public Domain"]
input:
- meta:
@ -22,6 +22,11 @@ input:
type: directory
description: Directory containing ETL data for the given SRA.
pattern: "*/*.sra"
- ncbi_settings:
type: file
description: >
An NCBI user settings file.
pattern: "*.mkfg"
output:
- meta:

5
modules/sratools/prefetch/main.nf
View file

@ -9,10 +9,11 @@ process SRATOOLS_PREFETCH {
input:
tuple val(meta), val(id)
path ncbi_settings
output:
tuple val(meta), path(id), emit: sra
path "versions.yml" , emit: versions
path 'versions.yml' , emit: versions
when:
task.ext.when == null || task.ext.when
@ -20,7 +21,5 @@ process SRATOOLS_PREFETCH {
shell:
args = task.ext.args ?: ''
args2 = task.ext.args2 ?: '5 1 100' // <num retries> <base delay in seconds> <max delay in seconds>
config = "/LIBS/GUID = \"${UUID.randomUUID().toString()}\"\\n/libs/cloud/report_instance_identity = \"true\"\\n"
template 'retry_with_backoff.sh'
}

7
modules/sratools/prefetch/meta.yml
View file

@ -10,7 +10,7 @@ tools:
homepage: https://github.com/ncbi/sra-tools
documentation: https://github.com/ncbi/sra-tools/wiki
tool_dev_url: https://github.com/ncbi/sra-tools
licence: ["US-Government-Work"]
licence: ["Public Domain"]
input:
- meta:
@ -22,6 +22,11 @@ input:
type: val
description: >
A string denoting an SRA id.
- ncbi_settings:
type: file
description: >
An NCBI user settings file.
pattern: "*.mkfg"
output:
- meta:

6
modules/sratools/prefetch/templates/retry_with_backoff.sh
View file

@ -40,11 +40,7 @@ retry_with_backoff() {
echo "${output}"
}
eval "$(vdb-config -o n NCBI_SETTINGS | sed 's/[" ]//g')"
if [[ ! -f "${NCBI_SETTINGS}" ]]; then
mkdir -p "$(dirname "${NCBI_SETTINGS}")"
printf '!{config}' > "${NCBI_SETTINGS}"
fi
export NCBI_SETTINGS="$PWD/!{ncbi_settings}"
retry_with_backoff !{args2} \
prefetch \

34
subworkflows/nf-core/sra_fastq/main.nf
View file

@ -1,34 +0,0 @@
//
// Download FASTQ sequencing reads from the NCBI's Sequence Read Archive (SRA).
//
params.prefetch_options = [:]
params.fasterqdump_options = [:]
include { SRATOOLS_PREFETCH } from '../../../modules/sratools/prefetch/main' addParams( options: params.prefetch_options )
include { SRATOOLS_FASTERQDUMP } from '../../../modules/sratools/fasterqdump/main' addParams( options: params.fasterqdump_options )
workflow SRA_FASTQ {
take:
sra_ids // channel: [ val(meta), val(id) ]
main:
ch_versions = Channel.empty()
//
// Prefetch sequencing reads in SRA format.
//
SRATOOLS_PREFETCH ( sra_ids )
ch_versions = ch_versions.mix( SRATOOLS_PREFETCH.out.versions.first() )
//
// Convert the SRA format into one or more compressed FASTQ files.
//
SRATOOLS_FASTERQDUMP ( SRATOOLS_PREFETCH.out.sra )
ch_versions = ch_versions.mix( SRATOOLS_FASTERQDUMP.out.versions.first() )
emit:
reads = SRATOOLS_FASTERQDUMP.out.reads // channel: [ val(meta), [ reads ] ]
versions = ch_versions // channel: [ versions.yml ]
}

38
subworkflows/nf-core/srafastq/main.nf Normal file
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@ -0,0 +1,38 @@
include { CUSTOM_SRATOOLSNCBISETTINGS } from '../../../modules/custom/sratoolsncbisettings/main'
include { SRATOOLS_PREFETCH } from '../../../modules/sratools/prefetch/main'
include { SRATOOLS_FASTERQDUMP } from '../../../modules/sratools/fasterqdump/main'
/**
* Download FASTQ sequencing reads from the NCBI's Sequence Read Archive (SRA).
*/
workflow SRAFASTQ {
take:
sra_ids // channel: [ val(meta), val(id) ]
main:
ch_versions = Channel.empty()
//
// Detect existing NCBI user settings or create new ones.
//
CUSTOM_SRATOOLSNCBISETTINGS()
def settings = CUSTOM_SRATOOLSNCBISETTINGS.out.ncbi_settings
ch_versions = ch_versions.mix( CUSTOM_SRATOOLSNCBISETTINGS.out.versions )
//
// Prefetch sequencing reads in SRA format.
//
SRATOOLS_PREFETCH ( sra_ids, settings )
ch_versions = ch_versions.mix( SRATOOLS_PREFETCH.out.versions.first() )
//
// Convert the SRA format into one or more compressed FASTQ files.
//
SRATOOLS_FASTERQDUMP ( SRATOOLS_PREFETCH.out.sra, settings )
ch_versions = ch_versions.mix( SRATOOLS_FASTERQDUMP.out.versions.first() )
emit:
reads = SRATOOLS_FASTERQDUMP.out.reads // channel: [ val(meta), [ reads ] ]
versions = ch_versions // channel: [ versions.yml ]
}

7
subworkflows/nf-core/sra_fastq/meta.yml → subworkflows/nf-core/srafastq/meta.yml
View file

@ -1,11 +1,14 @@
name: sra_fastq
description: Download FASTQ sequencing reads from the NCBI's Sequence Read Archive (SRA).
keywords:
- SRA
- NCBI
- sequencing
- FASTQ
- prefetch
- dump
- fasterq-dump
modules:
- custom/sratoolsncbisettings
- sratools/prefetch
- sratools/fasterqdump
input:
@ -17,7 +20,7 @@ input:
- id:
type: string
description: >
SRA identifier.
SRA run identifier.
# TODO Update when we decide on a standard for subworkflow docs
output:
- meta:

0
subworkflows/nf-core/sra_fastq/nextflow.config → subworkflows/nf-core/srafastq/nextflow.config
View file

8
tests/config/pytest_modules.yml
View file

@ -495,6 +495,10 @@ custom/getchromsizes:
- modules/custom/getchromsizes/**
- tests/modules/custom/getchromsizes/**
custom/sratoolsncbisettings:
- modules/custom/sratoolsncbisettings/**
- tests/modules/custom/sratoolsncbisettings/**
cutadapt:
- modules/cutadapt/**
- tests/modules/cutadapt/**
@ -1619,6 +1623,10 @@ rseqc/tin:
- modules/rseqc/tin/**
- tests/modules/rseqc/tin/**
rtgtools/vcfeval:
- modules/rtgtools/vcfeval/**
- tests/modules/rtgtools/vcfeval/**
salmon/index:
- modules/salmon/index/**
- tests/modules/salmon/index/**

11
tests/config/test_data.config
View file

@ -135,6 +135,7 @@ params {
transcriptome_fasta = "${test_data_dir}/genomics/homo_sapiens/genome/transcriptome.fasta"
genome2_fasta = "${test_data_dir}/genomics/homo_sapiens/genome/genome2.fasta"
genome_chain_gz = "${test_data_dir}/genomics/homo_sapiens/genome/genome.chain.gz"
genome_21_sdf = "${test_data_dir}/genomics/homo_sapiens/genome/chr21/sequence/genome_sdf.tar.gz"
genome_21_fasta = "${test_data_dir}/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta"
genome_21_fasta_fai = "${test_data_dir}/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai"
genome_21_dict = "${test_data_dir}/genomics/homo_sapiens/genome/chr21/sequence/genome.dict"
@ -212,7 +213,6 @@ params {
test_paired_end_hla = "${test_data_dir}/genomics/homo_sapiens/illumina/bam/example_hla_pe.bam"
test_paired_end_hla_sorted_bam = "${test_data_dir}/genomics/homo_sapiens/illumina/bam/example_hla_pe.sorted.bam"
test_paired_end_hla_sorted_bam_bai = "${test_data_dir}/genomics/homo_sapiens/illumina/bam/example_hla_pe.sorted.bam.bai"
test2_paired_end_sorted_bam = "${test_data_dir}/genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam"
test2_paired_end_sorted_bam_bai = "${test_data_dir}/genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam.bai"
test2_paired_end_name_sorted_bam = "${test_data_dir}/genomics/homo_sapiens/illumina/bam/test2.paired_end.name.sorted.bam"
@ -227,6 +227,7 @@ params {
test2_paired_end_umi_unsorted_bam = "${test_data_dir}/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_unsorted.bam"
test2_paired_end_umi_unsorted_tagged_bam = "${test_data_dir}/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.unsorted_tagged.bam"
mitochon_standin_recalibrated_sorted_bam = "${test_data_dir}/genomics/homo_sapiens/illumina/bam/mitochon_standin.recalibrated.sorted.bam"
mitochon_standin_recalibrated_sorted_bam_bai = "${test_data_dir}/genomics/homo_sapiens/illumina/bam/mitochon_standin.recalibrated.sorted.bam.bai"
@ -266,6 +267,9 @@ params {
test2_haplotc_ann_vcf_gz = "${test_data_dir}/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz"
test2_haplotc_ann_vcf_gz_tbi = "${test_data_dir}/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz.tbi"
test2_haplotc_vcf_gz = "${test_data_dir}/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz"
test2_haplotc_vcf_gz_tbi = "${test_data_dir}/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz.tbi"
test2_recal = "${test_data_dir}/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.recal"
test2_recal_idx = "${test_data_dir}/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.recal.idx"
test2_tranches = "${test_data_dir}/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.tranches"
@ -378,7 +382,7 @@ params {
test3_gff = "${test_data_dir}/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/genome/gff/test3.gff"
}
'illumina' {
test_1_fastq_gz = "${test_data_dir}/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/illumina/fasta/test_1.fastq.gz"
test_1_fastq_gz = "${test_data_dir}/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/illumina/fastq/test_1.fastq.gz"
test_2_fastq_gz = "${test_data_dir}/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/illumina/fastq/test_2.fastq.gz"
test_se_fastq_gz = "${test_data_dir}/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/illumina/fastq/test_se.fastq.gz"
}
@ -422,6 +426,9 @@ params {
test_merge_cool_cp2 = "${test_data_dir}/genomics/homo_sapiens/cooler/merge/toy/toy.symm.upper.2.cp2.cool"
}
'config' {
ncbi_user_settings = "${test_data_dir}/generic/config/ncbi_user_settings.mkfg"
}
}
}
}

4
tests/modules/arriba/main.nf
View file

@ -20,7 +20,7 @@ workflow test_arriba_single_end {
STAR_GENOMEGENERATE ( fasta, gtf )
STAR_ALIGN ( input, STAR_GENOMEGENERATE.out.index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
ARRIBA ( STAR_ALIGN.out.bam, fasta, gtf )
ARRIBA ( STAR_ALIGN.out.bam, fasta, gtf , [], [], [], [], [])
}
workflow test_arriba_paired_end {
@ -38,5 +38,5 @@ workflow test_arriba_paired_end {
STAR_GENOMEGENERATE ( fasta, gtf )
STAR_ALIGN ( input, STAR_GENOMEGENERATE.out.index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center )
ARRIBA ( STAR_ALIGN.out.bam, fasta, gtf )
ARRIBA ( STAR_ALIGN.out.bam, fasta, gtf, [], [], [], [], [])
}

5
tests/modules/arriba/test.yml
View file

@ -4,7 +4,7 @@
- arriba
files:
- path: output/arriba/test.fusions.discarded.tsv
md5sum: cad8c215b938d1e45b747a5b7898a4c2
md5sum: 7602ab4ccbbb0c54fbca12a942877e6d
- path: output/arriba/test.fusions.tsv
md5sum: 7c3383f7eb6d79b84b0bd30a7ef02d70
- path: output/star/star/Genome
@ -39,6 +39,7 @@
- path: output/star/star/transcriptInfo.tab
md5sum: 0c3a5adb49d15e5feff81db8e29f2e36
- path: output/star/test.Aligned.out.bam
md5sum: 4fa079d11f8938e51015e3e477fa7149
- path: output/star/test.Log.final.out
- path: output/star/test.Log.out
- path: output/star/test.Log.progress.out
@ -50,7 +51,7 @@
- arriba
files:
- path: output/arriba/test.fusions.discarded.tsv
md5sum: 85e36c887464e4deaa65f45174d3b8fd
md5sum: cdc6cfbc75e68ce29a766f50f390274d
- path: output/arriba/test.fusions.tsv
md5sum: 7c3383f7eb6d79b84b0bd30a7ef02d70
- path: output/star/star/Genome

84
tests/modules/bowtie2/align/test.yml
View file

@ -1,83 +1,21 @@
- name: bowtie2 align single-end
- name: bowtie2 align test_bowtie2_align_single_end
command: nextflow run ./tests/modules/bowtie2/align -entry test_bowtie2_align_single_end -c ./tests/config/nextflow.config -c ./tests/modules/bowtie2/align/nextflow.config
tags:
- bowtie2
- bowtie2/align
- bowtie2
files:
- path: ./output/bowtie2/test.bam
- path: ./output/bowtie2/test.bowtie2.log
- path: ./output/bowtie2/bowtie2/genome.3.bt2
md5sum: 4ed93abba181d8dfab2e303e33114777
- path: ./output/bowtie2/bowtie2/genome.2.bt2
md5sum: 47b153cd1319abc88dda532462651fcf
- path: ./output/bowtie2/bowtie2/genome.1.bt2
md5sum: cbe3d0bbea55bc57c99b4bfa25b5fbdf
- path: ./output/bowtie2/bowtie2/genome.4.bt2
md5sum: c25be5f8b0378abf7a58c8a880b87626
- path: ./output/bowtie2/bowtie2/genome.rev.1.bt2
md5sum: 52be6950579598a990570fbcf5372184
- path: ./output/bowtie2/bowtie2/genome.rev.2.bt2
md5sum: e3b4ef343dea4dd571642010a7d09597
- path: output/bowtie2/test.bam
- path: output/bowtie2/test.bowtie2.log
md5sum: 7b8a9e61b7646da1089b041333c41a87
- path: output/bowtie2/versions.yml
- name: bowtie2 align paired-end
- name: bowtie2 align test_bowtie2_align_paired_end
command: nextflow run ./tests/modules/bowtie2/align -entry test_bowtie2_align_paired_end -c ./tests/config/nextflow.config -c ./tests/modules/bowtie2/align/nextflow.config
tags:
- bowtie2
- bowtie2/align
files:
- path: ./output/bowtie2/test.bam
- path: ./output/bowtie2/test.bowtie2.log
- path: ./output/bowtie2/bowtie2/genome.3.bt2
md5sum: 4ed93abba181d8dfab2e303e33114777
- path: ./output/bowtie2/bowtie2/genome.2.bt2
md5sum: 47b153cd1319abc88dda532462651fcf
- path: ./output/bowtie2/bowtie2/genome.1.bt2
md5sum: cbe3d0bbea55bc57c99b4bfa25b5fbdf
- path: ./output/bowtie2/bowtie2/genome.4.bt2
md5sum: c25be5f8b0378abf7a58c8a880b87626
- path: ./output/bowtie2/bowtie2/genome.rev.1.bt2
md5sum: 52be6950579598a990570fbcf5372184
- path: ./output/bowtie2/bowtie2/genome.rev.2.bt2
md5sum: e3b4ef343dea4dd571642010a7d09597
- name: bowtie2 align single-end large-index
command: nextflow run ./tests/modules/bowtie2/align -entry test_bowtie2_align_single_end -c ./tests/config/nextflow.config -c ./tests/modules/bowtie2/align/nextflow.config --force_large_index
tags:
- bowtie2
- bowtie2/align
files:
- path: ./output/bowtie2/test.bam
- path: ./output/bowtie2/test.bowtie2.log
- path: ./output/bowtie2/bowtie2/genome.3.bt2l
md5sum: 8952b3e0b1ce9a7a5916f2e147180853
- path: ./output/bowtie2/bowtie2/genome.2.bt2l
md5sum: 22c284084784a0720989595e0c9461fd
- path: ./output/bowtie2/bowtie2/genome.1.bt2l
md5sum: 07d811cd4e350d56267183d2ac7023a5
- path: ./output/bowtie2/bowtie2/genome.4.bt2l
md5sum: c25be5f8b0378abf7a58c8a880b87626
- path: ./output/bowtie2/bowtie2/genome.rev.1.bt2l
md5sum: fda48e35925fb24d1c0785f021981e25
- path: ./output/bowtie2/bowtie2/genome.rev.2.bt2l
md5sum: 802c26d32b970e1b105032b7ce7348b4
- name: bowtie2 align paired-end large-index
command: nextflow run ./tests/modules/bowtie2/align -entry test_bowtie2_align_paired_end -c ./tests/config/nextflow.config -c ./tests/modules/bowtie2/align/nextflow.config --force_large_index
tags:
- bowtie2
- bowtie2/align
files:
- path: ./output/bowtie2/test.bam
- path: ./output/bowtie2/test.bowtie2.log
- path: ./output/bowtie2/bowtie2/genome.3.bt2l
md5sum: 8952b3e0b1ce9a7a5916f2e147180853
- path: ./output/bowtie2/bowtie2/genome.2.bt2l
md5sum: 22c284084784a0720989595e0c9461fd
- path: ./output/bowtie2/bowtie2/genome.1.bt2l
md5sum: 07d811cd4e350d56267183d2ac7023a5
- path: ./output/bowtie2/bowtie2/genome.4.bt2l
md5sum: c25be5f8b0378abf7a58c8a880b87626
- path: ./output/bowtie2/bowtie2/genome.rev.1.bt2l
md5sum: fda48e35925fb24d1c0785f021981e25
- path: ./output/bowtie2/bowtie2/genome.rev.2.bt2l
md5sum: 802c26d32b970e1b105032b7ce7348b4
- path: output/bowtie2/test.bam
- path: output/bowtie2/test.bowtie2.log
md5sum: bd89ce1b28c93bf822bae391ffcedd19
- path: output/bowtie2/versions.yml

44
tests/modules/custom/sratoolsncbisettings/main.nf Normal file
View file

@ -0,0 +1,44 @@
#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
include { CUSTOM_SRATOOLSNCBISETTINGS } from '../../../../modules/custom/sratoolsncbisettings/main.nf'
workflow test_sratoolsncbisettings_with_good_existing {
file(params.settings_path).mkdirs()
def settings = file(params.test_data['generic']['config']['ncbi_user_settings'], checkIfExists: true)
settings.copyTo(params.settings_file)
CUSTOM_SRATOOLSNCBISETTINGS()
}
workflow test_sratoolsncbisettings_with_bad_existing {
file(params.settings_path).mkdirs()
def settings = file(params.settings_file)
settings.text = '''
## auto-generated configuration file - DO NOT EDIT ##
config/default = "false"
/repository/remote/main/CGI/resolver-cgi = "https://trace.ncbi.nlm.nih.gov/Traces/names/names.fcgi"
/repository/remote/protected/CGI/resolver-cgi = "https://trace.ncbi.nlm.nih.gov/Traces/names/names.fcgi"
/repository/user/ad/public/apps/file/volumes/flatAd = "."
/repository/user/ad/public/apps/refseq/volumes/refseqAd = "."
/repository/user/ad/public/apps/sra/volumes/sraAd = "."
/repository/user/ad/public/apps/sraPileup/volumes/ad = "."
/repository/user/ad/public/apps/sraRealign/volumes/ad = "."
/repository/user/ad/public/apps/wgs/volumes/wgsAd = "."
/repository/user/ad/public/root = "."
/repository/user/default-path = "/root/ncbi"
'''.stripIndent()
CUSTOM_SRATOOLSNCBISETTINGS()
}
workflow test_sratoolsncbisettings_with_nonexisting {
def settings = file(params.settings_file)
settings.delete()
CUSTOM_SRATOOLSNCBISETTINGS()
}

8
tests/modules/custom/sratoolsncbisettings/nextflow.config Normal file
View file

@ -0,0 +1,8 @@
params.settings_path = '/tmp/.ncbi'
params.settings_file = "${params.settings_path}/user-settings.mkfg"
process {
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
}

17
tests/modules/custom/sratoolsncbisettings/nextflow_mount.config Normal file
View file

@ -0,0 +1,17 @@
params.settings_path = '/tmp/.ncbi'
params.settings_file = "${params.settings_path}/user-settings.mkfg"
env.NCBI_SETTINGS = params.settings_file
process {
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
withName: CUSTOM_SRATOOLSNCBISETTINGS {
containerOptions = {
(workflow.containerEngine == 'singularity') ?
"-B ${params.settings_path}:${params.settings_path}" :
"-v ${params.settings_path}:${params.settings_path}"
}
}
}

44
tests/modules/custom/sratoolsncbisettings/test.yml Normal file
View file

@ -0,0 +1,44 @@
- name: "custom sratoolsncbisettings test_sratoolsncbisettings_with_good_existing"
command: nextflow run ./tests/modules/custom/sratoolsncbisettings -entry test_sratoolsncbisettings_with_good_existing -c ./tests/config/nextflow.config -c ./tests/modules/custom/sratoolsncbisettings/nextflow_mount.config
tags:
- "custom"
- "custom/sratoolsncbisettings"
files:
- path: "output/custom/user-settings.mkfg"
md5sum: 955e27aff2c277c2f1f0943a098888c1
- path: output/custom/versions.yml
contains:
- "sratools: 2.11.0"
- name: "custom sratoolsncbisettings test_sratoolsncbisettings_with_bad_existing"
command: nextflow run ./tests/modules/custom/sratoolsncbisettings -entry test_sratoolsncbisettings_with_bad_existing -c ./tests/config/nextflow.config -c ./tests/modules/custom/sratoolsncbisettings/nextflow_mount.config
tags:
- "custom"
- "custom/sratoolsncbisettings"
exit_code: 1
stdout:
contains:
- "Command error:"
- "missing the required entries"
- "/LIBS/GUID"
- "/libs/cloud/report_instance_identity"
- "Feel free to add the following"
files:
- path: "output/custom/user-settings.mkfg"
should_exist: false
- path: output/custom/versions.yml
should_exist: false
- name: "custom sratoolsncbisettings test_sratoolsncbisettings_with_nonexisting"
command: nextflow run ./tests/modules/custom/sratoolsncbisettings -entry test_sratoolsncbisettings_with_nonexisting -c ./tests/config/nextflow.config -c ./tests/modules/custom/sratoolsncbisettings/nextflow.config
tags:
- "custom"
- "custom/sratoolsncbisettings"
files:
- path: "output/custom/user-settings.mkfg"
contains:
- "/LIBS/GUID"
- "/libs/cloud/report_instance_identity"
- path: output/custom/versions.yml
contains:
- "sratools: 2.11.0"

69
tests/modules/rtgtools/vcfeval/main.nf Normal file
View file

@ -0,0 +1,69 @@
#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
include { RTGTOOLS_VCFEVAL } from '../../../../modules/rtgtools/vcfeval/main.nf'
include { UNTAR } from '../../../modules/untar/main.nf'
workflow test_rtgtools_vcfeval {
input = [
[ id:'test' ], // meta map
file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true),
file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz_tbi'], checkIfExists: true),
]
truth = [
file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_ann_vcf_gz'], checkIfExists: true),
file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_ann_vcf_gz_tbi'], checkIfExists: true)
]
truth_regions = file(params.test_data['homo_sapiens']['genome']['genome_21_multi_interval_bed'], checkIfExists: true)
evaluation_regions = file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true)
compressed_sdf = [
[],
file(params.test_data['homo_sapiens']['genome']['genome_21_sdf'])
]
sdf = UNTAR( compressed_sdf ).untar
.map({
meta, folder ->
folder
})
RTGTOOLS_VCFEVAL ( input, truth, truth_regions, evaluation_regions, sdf )
}
workflow test_rtgtools_vcfeval_no_optional_inputs {
input = [
[ id:'test' ], // meta map
file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true),
[],
]
truth = [
file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_ann_vcf_gz'], checkIfExists: true),
[]
]
truth_regions = []
evaluation_regions = []
compressed_sdf = [
[],
file(params.test_data['homo_sapiens']['genome']['genome_21_sdf'])
]
sdf = UNTAR( compressed_sdf ).untar
.map({
meta, folder ->
[folder]
})
RTGTOOLS_VCFEVAL ( input, truth, truth_regions, evaluation_regions, sdf )
}

5
tests/modules/rtgtools/vcfeval/nextflow.config Normal file
View file

@ -0,0 +1,5 @@
process {
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
}

75
tests/modules/rtgtools/vcfeval/test.yml Normal file
View file

@ -0,0 +1,75 @@
- name: rtgtools vcfeval test_rtgtools_vcfeval
command: nextflow run tests/modules/rtgtools/vcfeval -entry test_rtgtools_vcfeval -c tests/config/nextflow.config
tags:
- rtgtools
- rtgtools/vcfeval
files:
- path: output/rtgtools/test_results/done
- path: output/rtgtools/test_results/fn.vcf.gz
md5sum: be9c9106055bfad4c5985bc0d33efd56
- path: output/rtgtools/test_results/fn.vcf.gz.tbi
md5sum: 092a7a3162e7cff25d273525751eb284
- path: output/rtgtools/test_results/fp.vcf.gz
md5sum: e0f0ff841dc63e9fb61fd3a5db137ced
- path: output/rtgtools/test_results/fp.vcf.gz.tbi
md5sum: 092a7a3162e7cff25d273525751eb284
- path: output/rtgtools/test_results/non_snp_roc.tsv.gz
md5sum: ad5bad32c48f05aef232e2c0e708877a
- path: output/rtgtools/test_results/phasing.txt
md5sum: 133677dbd8be657439ea2b03fdfb8795
- path: output/rtgtools/test_results/progress
- path: output/rtgtools/test_results/snp_roc.tsv.gz
md5sum: 6785b83d66486e7e6c75c5a5b1574c09
- path: output/rtgtools/test_results/summary.txt
md5sum: f4c8df93c8bdab603036bbc27b4a28c3
- path: output/rtgtools/test_results/tp-baseline.vcf.gz
md5sum: be9c9106055bfad4c5985bc0d33efd56
- path: output/rtgtools/test_results/tp-baseline.vcf.gz.tbi
md5sum: 092a7a3162e7cff25d273525751eb284
- path: output/rtgtools/test_results/tp.vcf.gz
md5sum: e0f0ff841dc63e9fb61fd3a5db137ced
- path: output/rtgtools/test_results/tp.vcf.gz.tbi
md5sum: 092a7a3162e7cff25d273525751eb284
- path: output/rtgtools/test_results/vcfeval.log
- path: output/rtgtools/test_results/weighted_roc.tsv.gz
md5sum: fa7c046ea0084172f1ef91f19de07b2b
- path: output/rtgtools/versions.yml
md5sum: 270ed7a5a8e347b251eb4aa2198f98e8
- name: rtgtools vcfeval test_rtgtools_vcfeval_no_optional_inputs
command: nextflow run tests/modules/rtgtools/vcfeval -entry test_rtgtools_vcfeval_no_optional_inputs -c tests/config/nextflow.config
tags:
- rtgtools
- rtgtools/vcfeval
files:
- path: output/rtgtools/test_results/done
- path: output/rtgtools/test_results/fn.vcf.gz
md5sum: c11c889a4f42c8ea325748bd768ea34d
- path: output/rtgtools/test_results/fn.vcf.gz.tbi
md5sum: 092a7a3162e7cff25d273525751eb284
- path: output/rtgtools/test_results/fp.vcf.gz
md5sum: 138e85c1cd79f8fea9a33e81ce0c734c
- path: output/rtgtools/test_results/fp.vcf.gz.tbi
md5sum: 092a7a3162e7cff25d273525751eb284
- path: output/rtgtools/test_results/non_snp_roc.tsv.gz
md5sum: 34fb78a008dfc0bef02807b8a7012b07
- path: output/rtgtools/test_results/phasing.txt
md5sum: 133677dbd8be657439ea2b03fdfb8795
- path: output/rtgtools/test_results/progress
- path: output/rtgtools/test_results/snp_roc.tsv.gz
md5sum: a4c5761c2653e2d04fc84c1cea13b1f0
- path: output/rtgtools/test_results/summary.txt
md5sum: f33feb32f84958fb931063044fba369b
- path: output/rtgtools/test_results/tp-baseline.vcf.gz
md5sum: d1c2d990899edf127ea5fcca8866fcb0
- path: output/rtgtools/test_results/tp-baseline.vcf.gz.tbi
md5sum: 3307008fea47adb75c46d395c5567bc0
- path: output/rtgtools/test_results/tp.vcf.gz
md5sum: e35b4dab82894eee9b77c81f9bc89cca
- path: output/rtgtools/test_results/tp.vcf.gz.tbi
md5sum: 45d8f8793140944f129e728299918c88
- path: output/rtgtools/test_results/vcfeval.log
- path: output/rtgtools/test_results/weighted_roc.tsv.gz
md5sum: 5b8efc9e9381f604880412800f58e4e9
- path: output/rtgtools/versions.yml
md5sum: 55568e4bbe5ab7e634a1f392abb89cc4

4
tests/modules/sratools/fasterqdump/main.nf
View file

@ -13,7 +13,7 @@ workflow test_sratools_fasterqdump_single_end {
def input = Channel.of([ id:'test_single_end', single_end:true ])
.combine(UNTAR.out.untar.map{ it[1] })
SRATOOLS_FASTERQDUMP ( input )
SRATOOLS_FASTERQDUMP(input, file(params.test_data['generic']['config']['ncbi_user_settings'], checkIfExists: true))
}
workflow test_sratools_fasterqdump_paired_end {
@ -24,5 +24,5 @@ workflow test_sratools_fasterqdump_paired_end {
def input = Channel.of([ id:'test_paired_end', single_end:false ])
.combine(UNTAR.out.untar.map{ it[1] })
SRATOOLS_FASTERQDUMP ( input )
SRATOOLS_FASTERQDUMP(input, file(params.test_data['generic']['config']['ncbi_user_settings'], checkIfExists: true))
}

6
tests/modules/sratools/fasterqdump/test.yml
View file

@ -8,6 +8,9 @@
md5sum: 1054c7b71884acdb5eed8a378f18be82
- path: output/untar/SRR13255544/SRR13255544.sra
md5sum: 466d05dafb2eec672150754168010b4d
- path: output/sratools/versions.yml
contains:
- "sratools: 2.11.0"
- name: sratools fasterqdump test_sratools_fasterqdump_paired_end
command: nextflow run ./tests/modules/sratools/fasterqdump -entry test_sratools_fasterqdump_paired_end -c ./tests/config/nextflow.config -c ./tests/modules/sratools/fasterqdump/nextflow.config
@ -21,3 +24,6 @@
md5sum: 3e3b3af3413f50a1685fd7b3f1456d4e
- path: output/untar/SRR11140744/SRR11140744.sra
md5sum: 065666caf5b2d5dfb0cb25d5f3abe659
- path: output/sratools/versions.yml
contains:
- "sratools: 2.11.0"

4
tests/modules/sratools/prefetch/main.nf
View file

@ -8,8 +8,8 @@ workflow test_sratools_prefetch {
input = [
[ id:'test', single_end:false ], // meta map
'ERR2815334'
'DRR000774'
]
SRATOOLS_PREFETCH ( input )
SRATOOLS_PREFETCH(input, file(params.test_data['generic']['config']['ncbi_user_settings'], checkIfExists: true))
}

7
tests/modules/sratools/prefetch/test.yml
View file

@ -4,5 +4,8 @@
- sratools/prefetch
- sratools
files:
- path: output/sratools/ERR2815334/ERR2815334.sra
md5sum: 9a98c7f6f4774b7ef94aa915b92a54ea
- path: output/sratools/DRR000774/DRR000774.sra
md5sum: 7647dba20c89c0e3d7ad13842f060eb0
- path: output/sratools/versions.yml
contains:
- "sratools: 2.11.0"

23
tests/subworkflows/nf-core/sra_fastq/main.nf
View file

@ -1,23 +0,0 @@
#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
include { SRA_FASTQ } from '../../../../subworkflows/nf-core/sra_fastq/main.nf' addParams( [:] )
workflow test_sra_fastq_single_end {
input = [
[ id:'test_single_end', single_end:true ], // meta map
'SRR13255544'
]
SRA_FASTQ ( input )
}
workflow test_sra_fastq_paired_end {
input = [
[ id:'test_paired_end', single_end:false ], // meta map
'SRR11140744'
]
SRA_FASTQ ( input )
}

27
tests/subworkflows/nf-core/sra_fastq/test.yml
View file

@ -1,27 +0,0 @@
- name: sra fastq single-end
command: nextflow run ./tests/subworkflows/nf-core/sra_fastq -entry test_sra_fastq_single_end -c tests/config/nextflow.config
tags:
- subworkflows
# - subworkflows/sra_fastq
# Modules
# - sratools
# - sratools/prefetch
# - sratools/fasterqdump
files:
- path: output/sratools/SRR13255544.fastq.gz
md5sum: 1054c7b71884acdb5eed8a378f18be82
- name: sra fastq paired-end
command: nextflow run ./tests/subworkflows/nf-core/sra_fastq -entry test_sra_fastq_paired_end -c tests/config/nextflow.config
tags:
- subworkflows
# - subworkflows/sra_fastq
# Modules
# - sratools
# - sratools/prefetch
# - sratools/fasterqdump
files:
- path: output/sratools/SRR11140744_1.fastq.gz
md5sum: 193809c784a4ea132ab2a253fa4f55b6
- path: output/sratools/SRR11140744_2.fastq.gz
md5sum: 3e3b3af3413f50a1685fd7b3f1456d4e

29
tests/subworkflows/nf-core/srafastq/main.nf Normal file
View file

@ -0,0 +1,29 @@
#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
include { SRAFASTQ } from '../../../../subworkflows/nf-core/srafastq/main.nf'
workflow test_srafastq_single_end {
input = Channel.of(
[
[ id:'test_single_end1', single_end:true ], // meta map
'DRR000774'
],
[
[ id:'test_single_end2', single_end:true ], // meta map
'DRR000775'
]
)
SRAFASTQ ( input )
}
workflow test_srafastq_paired_end {
input = [
[ id:'test_paired_end', single_end:false ], // meta map
'SRR11140744'
]
SRAFASTQ ( input )
}

5
tests/subworkflows/nf-core/srafastq/nextflow.config Normal file
View file

@ -0,0 +1,5 @@
process {
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
}

29
tests/subworkflows/nf-core/srafastq/test.yml Normal file
View file

@ -0,0 +1,29 @@
- name: srafastq single-end
command: nextflow run ./tests/subworkflows/nf-core/srafastq -entry test_srafastq_single_end -c tests/config/nextflow.config -c tests/subworkflows/nf-core/srafastq/nextflow.config
tags:
- subworkflows
# - subworkflows/srafastq
# Modules
# - sratools
# - sratools/prefetch
# - sratools/fasterqdump
files:
- path: output/sratools/DRR000774.fastq.gz
md5sum: 19029a1132115b55277a0d79ee089b49
- path: output/sratools/DRR000775.fastq.gz
md5sum: 59ff24c86ecb260752668c059c2a1eaf
- name: srafastq paired-end
command: nextflow run ./tests/subworkflows/nf-core/srafastq -entry test_srafastq_paired_end -c tests/config/nextflow.config -c tests/subworkflows/nf-core/srafastq/nextflow.config
tags:
- subworkflows
# - subworkflows/srafastq
# Modules
# - sratools
# - sratools/prefetch
# - sratools/fasterqdump
files:
- path: output/sratools/SRR11140744_1.fastq.gz
md5sum: 193809c784a4ea132ab2a253fa4f55b6
- path: output/sratools/SRR11140744_2.fastq.gz
md5sum: 3e3b3af3413f50a1685fd7b3f1456d4e