Add Medaka module (#992)

* add racon

* add medaka module

* add medaka module

* add medaka module

* add medaka module

* add medaka module

* add medaka module

* Indentation

* Apply suggestions from code review

Co-authored-by: FriederikeHanssen <Friederike.hanssen@qbic.uni-tuebingen.de>

* Update main.nf

* Update main.nf

* Apply suggestions from code review

Co-authored-by: FriederikeHanssen <Friederike.hanssen@qbic.uni-tuebingen.de>
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
This commit is contained in:
avantonder 2021-11-15 17:26:37 +00:00 committed by GitHub
parent 4398056204
commit 8d9e8ae839
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6 changed files with 199 additions and 0 deletions

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//
// Utility functions used in nf-core DSL2 module files
//
//
// Extract name of software tool from process name using $task.process
//
def getSoftwareName(task_process) {
return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
}
//
// Extract name of module from process name using $task.process
//
def getProcessName(task_process) {
return task_process.tokenize(':')[-1]
}
//
// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
//
def initOptions(Map args) {
def Map options = [:]
options.args = args.args ?: ''
options.args2 = args.args2 ?: ''
options.args3 = args.args3 ?: ''
options.publish_by_meta = args.publish_by_meta ?: []
options.publish_dir = args.publish_dir ?: ''
options.publish_files = args.publish_files
options.suffix = args.suffix ?: ''
return options
}
//
// Tidy up and join elements of a list to return a path string
//
def getPathFromList(path_list) {
def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries
paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
return paths.join('/')
}
//
// Function to save/publish module results
//
def saveFiles(Map args) {
def ioptions = initOptions(args.options)
def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
// Do not publish versions.yml unless running from pytest workflow
if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) {
return null
}
if (ioptions.publish_by_meta) {
def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
for (key in key_list) {
if (args.meta && key instanceof String) {
def path = key
if (args.meta.containsKey(key)) {
path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
}
path = path instanceof String ? path : ''
path_list.add(path)
}
}
}
if (ioptions.publish_files instanceof Map) {
for (ext in ioptions.publish_files) {
if (args.filename.endsWith(ext.key)) {
def ext_list = path_list.collect()
ext_list.add(ext.value)
return "${getPathFromList(ext_list)}/$args.filename"
}
}
} else if (ioptions.publish_files == null) {
return "${getPathFromList(path_list)}/$args.filename"
}
}

47
modules/medaka/main.nf Normal file
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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions'
params.options = [:]
options = initOptions(params.options)
process MEDAKA {
tag "$meta.id"
label 'process_high'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
conda (params.enable_conda ? "bioconda::medaka=1.4.4" : null)
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
container "https://depot.galaxyproject.org/singularity/medaka:1.4.4--py38h130def0_0"
} else {
container "quay.io/biocontainers/medaka:1.4.4--py38h130def0_0"
}
input:
tuple val(meta), path(reads), path(assembly)
output:
tuple val(meta), path("*.fa.gz"), emit: assembly
path "versions.yml" , emit: versions
script:
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
"""
medaka_consensus \\
-t $task.cpus \\
$options.args \\
-i $reads \\
-d $assembly \\
-o ./
mv consensus.fasta ${prefix}.fa
gzip -n ${prefix}.fa
cat <<-END_VERSIONS > versions.yml
${getProcessName(task.process)}:
${getSoftwareName(task.process)}: \$( medaka --version 2>&1 | sed 's/medaka //g' )
END_VERSIONS
"""
}

47
modules/medaka/meta.yml Normal file
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name: medaka
description: A tool to create consensus sequences and variant calls from nanopore sequencing data
keywords:
- assembly
- polishing
- nanopore
tools:
- medaka:
description: Neural network sequence error correction.
homepage: https://nanoporetech.github.io/medaka/index.html
documentation: https://nanoporetech.github.io/medaka/index.html
tool_dev_url: https://github.com/nanoporetech/medaka
doi: ""
licence: ['Mozilla Public License 2.0']
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- reads:
type: file
description: List of input nanopore fasta/FastQ files
pattern: "*.{fasta,fa,fastq,fastq.gz,fq,fq.gz}"
- assembly:
type: file
description: Genome assembly
pattern: "*.{fasta,fa}"
output:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
- assembly:
type: file
description: Polished genome assembly
pattern: "*.fa.gz"
authors:
- "@avantonder"

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@ -822,6 +822,10 @@ maxbin2:
- modules/maxbin2/**
- tests/modules/maxbin2/**
medaka:
- modules/medaka/**
- tests/modules/medaka/**
megahit:
- modules/megahit/**
- tests/modules/megahit/**

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#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
include { MEDAKA } from '../../../modules/medaka/main.nf' addParams( options: [suffix:'.polished.genome'] )
workflow test_medaka {
input = [
[ id:'test', single_end:true ], // meta map
file(params.test_data['sarscov2']['nanopore']['test_fastq_gz'], checkIfExists: true),
file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
]
MEDAKA ( input )
}

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- name: medaka test_medaka
command: nextflow run ./tests/modules/medaka -entry test_medaka -c tests/config/nextflow.config
tags:
- medaka
files:
- path: output/medaka/test.polished.genome.fa.gz
md5sum: f42303f1d6c2c79175faeb00e10b9a6e