Merge branch 'master' into correcting_stubs

modules/bowtie2/align/main.nf

@@ -29,6 +29,8 @@ process BOWTIE2_ALIGN {
         def unaligned = save_unaligned ? "--un-gz ${prefix}.unmapped.fastq.gz" : ''
         """
         INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'`
+        [ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed 's/.rev.1.bt2l//'`
+        [ -z "\$INDEX" ] && echo "BT2 index files not found" 1>&2 && exit 1
         bowtie2 \\
             -x \$INDEX \\
             -U $reads \\
@@ -49,6 +51,8 @@ process BOWTIE2_ALIGN {
         def unaligned = save_unaligned ? "--un-conc-gz ${prefix}.unmapped.fastq.gz" : ''
         """
         INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'`
+        [ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed 's/.rev.1.bt2l//'`
+        [ -z "\$INDEX" ] && echo "BT2 index files not found" 1>&2 && exit 1
         bowtie2 \\
             -x \$INDEX \\
             -1 ${reads[0]} \\

modules/cnvpytor/callcnvs/main.nf

@@ -2,43 +2,42 @@ process CNVPYTOR_CALLCNVS {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::cnvpytor=1.0" : null)
+    conda (params.enable_conda ? "bioconda::cnvpytor=1.2.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cnvpytor:1.0--py39h6a678da_2':
-        'quay.io/biocontainers/cnvpytor:1.0--py39h6a678da_2' }"
+        'https://depot.galaxyproject.org/singularity/cnvpytor:1.2.1--pyhdfd78af_0':
+        'quay.io/biocontainers/cnvpytor:1.2.1--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(pytor)
+    val bin_sizes
 
     output:
-    tuple val(meta), path("*.tsv"), emit: cnvs
-    path "versions.yml"           , emit: versions
+    tuple val(meta), path("${pytor.baseName}.pytor")	, emit: pytor
+    path "versions.yml"                                 , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: '1000'
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    def bins = bin_sizes ?: '1000'
     """
     cnvpytor \\
         -root $pytor \\
-        -call $args > ${prefix}.tsv
+        -call $bin_sizes
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/^.*pyCNVnator //; s/Using.*\$//' ))
+        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/CNVpytor //' ))
     END_VERSIONS
     """
 
     stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    touch ${prefix}.tsv
+    touch ${pytor.baseName}.pytor
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/^.*pyCNVnator //; s/Using.*\$//' ))
+        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/CNVpytor //' ))
     END_VERSIONS
     """
 }

modules/cnvpytor/callcnvs/meta.yml

@@ -17,8 +17,11 @@ input:
         e.g. [ id:'test']
   - pytor:
       type: file
-      description: cnvpytor root file
+      description: pytor file containing partitions of read depth histograms using mean-shift method
       pattern: "*.{pytor}"
+  - bin_sizes:
+      type: string
+      description: list of binsizes separated by space e.g. "1000 10000" and "1000"
 
 output:
   - meta:
@@ -26,10 +29,10 @@ output:
       description: |
         Groovy Map containing sample information
         e.g. [ id:'test' ]
-  - cnvs:
+  - pytor:
       type: file
-      description: file containing identified copy numer variations
-      pattern: "*.{tsv}"
+      description: pytor files containing cnv calls
+      pattern: "*.{pytor}"
   - versions:
       type: file
       description: File containing software versions

modules/cnvpytor/histogram/main.nf

@@ -2,13 +2,15 @@ process CNVPYTOR_HISTOGRAM {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::cnvpytor=1.0" : null)
+    conda (params.enable_conda ? "bioconda::cnvpytor=1.2.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cnvpytor:1.0--py39h6a678da_2':
-        'quay.io/biocontainers/cnvpytor:1.0--py39h6a678da_2' }"
+        'https://depot.galaxyproject.org/singularity/cnvpytor:1.2.1--pyhdfd78af_0':
+        'quay.io/biocontainers/cnvpytor:1.2.1--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(pytor)
+    val bin_sizes
+
 
     output:
     tuple val(meta), path("${pytor.baseName}.pytor")	, emit: pytor
@@ -18,15 +20,15 @@ process CNVPYTOR_HISTOGRAM {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: '1000'
+    def bins = bin_sizes ?: '1000'
     """
     cnvpytor \\
         -root $pytor \\
-        -his $args
+        -his $bins
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/^.*pyCNVnator //; s/Using.*\$//' ))
+        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/CNVpytor //' ))
     END_VERSIONS
     """
 
@@ -36,7 +38,7 @@ process CNVPYTOR_HISTOGRAM {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/^.*pyCNVnator //; s/Using.*\$//' ))
+        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/CNVpytor //' ))
     END_VERSIONS
     """
 }

modules/cnvpytor/histogram/meta.yml

@@ -22,6 +22,9 @@ input:
       type: file
       description: pytor file containing read depth data
       pattern: "*.{pytor}"
+  - bin_sizes:
+      type: string
+      description: list of binsizes separated by space e.g. "1000 10000" and "1000"
 
 output:
   - meta:
@@ -40,3 +43,4 @@ output:
 
 authors:
   - "@sima-r"
+  - "@ramprasadn"

modules/cnvpytor/importreaddepth/main.nf

@@ -2,10 +2,10 @@ process CNVPYTOR_IMPORTREADDEPTH {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::cnvpytor=1.0" : null)
+    conda (params.enable_conda ? "bioconda::cnvpytor=1.2.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cnvpytor:1.0--py39h6a678da_2':
-        'quay.io/biocontainers/cnvpytor:1.0--py39h6a678da_2' }"
+        'https://depot.galaxyproject.org/singularity/cnvpytor:1.2.1--pyhdfd78af_0':
+        'quay.io/biocontainers/cnvpytor:1.2.1--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(input_file), path(index)
@@ -32,7 +32,7 @@ process CNVPYTOR_IMPORTREADDEPTH {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/^.*pyCNVnator //; s/Using.*\$//' ))
+        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/CNVpytor //' ))
     END_VERSIONS
     """
 
@@ -43,7 +43,7 @@ process CNVPYTOR_IMPORTREADDEPTH {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/^.*pyCNVnator //; s/Using.*\$//' ))
+        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/CNVpytor //' ))
     END_VERSIONS
     """
 }

modules/cnvpytor/importreaddepth/meta.yml

@@ -52,3 +52,4 @@ output:
 
 authors:
   - "@sima-r"
+  - "@ramprasadn"

modules/cnvpytor/partition/main.nf

@@ -2,13 +2,14 @@ process CNVPYTOR_PARTITION {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::cnvpytor=1.0" : null)
+    conda (params.enable_conda ? "bioconda::cnvpytor=1.2.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cnvpytor:1.0--py39h6a678da_2':
-        'quay.io/biocontainers/cnvpytor:1.0--py39h6a678da_2' }"
+        'https://depot.galaxyproject.org/singularity/cnvpytor:1.2.1--pyhdfd78af_0':
+        'quay.io/biocontainers/cnvpytor:1.2.1--pyhdfd78af_0' }"
 
     input:
     tuple val(meta), path(pytor)
+    val bin_sizes
 
     output:
     tuple val(meta), path("${pytor.baseName}.pytor"), emit: pytor
@@ -18,15 +19,15 @@ process CNVPYTOR_PARTITION {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
+    def bins = bin_sizes ?: '1000'
     """
     cnvpytor \\
         -root $pytor \\
-        -partition $args
+        -partition $bins
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/^.*pyCNVnator //; s/Using.*\$//' ))
+        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/CNVpytor //' ))
     END_VERSIONS
     """
 
@@ -36,7 +37,7 @@ process CNVPYTOR_PARTITION {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/^.*pyCNVnator //; s/Using.*\$//' ))
+        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/CNVpytor //' ))
     END_VERSIONS
     """
 }

modules/cnvpytor/partition/meta.yml

@@ -22,6 +22,9 @@ input:
       type: file
       description: pytor file containing read depth data
       pattern: "*.{pytor}"
+  - bin_sizes:
+      type: string
+      description: list of binsizes separated by space e.g. "1000 10000" and "1000"
 
 output:
   - meta:
@@ -40,3 +43,4 @@ output:
 
 authors:
   - "@sima-r"
+  - "@ramprasadn"

modules/cnvpytor/view/main.nf

@@ -0,0 +1,60 @@
+process CNVPYTOR_VIEW {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::cnvpytor=1.2.1" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/cnvpytor:1.2.1--pyhdfd78af_0':
+        'quay.io/biocontainers/cnvpytor:1.2.1--pyhdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(pytor_files)
+    val bin_sizes
+    val output_format
+
+    output:
+    tuple val(meta), path("*.vcf"), emit: vcf      , optional: true
+    tuple val(meta), path("*.tsv"), emit: tsv      , optional: true
+    tuple val(meta), path("*.xls"), emit: xls      , optional: true
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def output_suffix = output_format ?: 'vcf'
+    def bins   = bin_sizes ?: '1000'
+    def input  = pytor_files.join(" ")
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+
+    python3 <<CODE
+    import cnvpytor,os
+    binsizes = "${bins}".split(" ")
+    for binsize in binsizes:
+        file_list = "${input}".split(" ")
+        app = cnvpytor.Viewer(file_list, params={} )
+        outputfile = "{}_{}.{}".format("${prefix}",binsize.strip(),"${output_suffix}")
+        app.print_filename = outputfile
+        app.bin_size = int(binsize)
+        app.print_calls_file()
+    CODE
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/CNVpytor //' ))
+    END_VERSIONS
+    """
+
+    stub:
+    def output_suffix = output_format ?: 'vcf'
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.${output_suffix}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        cnvpytor: \$(echo \$(cnvpytor --version 2>&1) | sed 's/CNVpytor //' ))
+    END_VERSIONS
+    """
+}

modules/cnvpytor/view/meta.yml

@@ -0,0 +1,56 @@
+name: cnvpytor_view
+description: view function to generate vcfs
+keywords:
+  - cnv calling
+tools:
+  - cnvpytor:
+      description: calling CNVs using read depth
+      homepage: https://github.com/abyzovlab/CNVpytor
+      documentation: https://github.com/abyzovlab/CNVpytor
+      tool_dev_url: https://github.com/abyzovlab/CNVpytor
+      doi: "10.1101/2021.01.27.428472v1"
+      licence: ["MIT"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test' ]
+  - pytor_files:
+      type: file
+      description: pytor file containing cnv calls. To merge calls from multiple samples use a list of files.
+      pattern: "*.{pytor}"
+  - bin_sizes:
+      type: string
+      description: list of binsizes separated by space e.g. "1000 10000" and "1000"
+  - output_format:
+      type: string
+      description: output format of the cnv calls. Valid entries are "tsv", "vcf", and "xls"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test' ]
+  - tsv:
+      type: file
+      description: tsv file containing cnv calls
+      pattern: "*.{tsv}"
+  - vcf:
+      type: file
+      description: vcf file containing cnv calls
+      pattern: "*.{vcf}"
+  - xls:
+      type: file
+      description: xls file containing cnv calls
+      pattern: "*.{xls}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@sima-r"
+  - "@ramprasadn"

modules/ensemblvep/Dockerfile

@@ -8,13 +8,14 @@ LABEL \
 COPY environment.yml /
 RUN conda env create -f /environment.yml && conda clean -a
 
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-vep-104.3/bin:$PATH
-
 # Setup default ARG variables
 ARG GENOME=GRCh38
 ARG SPECIES=homo_sapiens
-ARG VEP_VERSION=99
+ARG VEP_VERSION=104
+ARG VEP_TAG=104.3
+
+# Add conda installation dir to PATH (instead of doing 'conda activate')
+ENV PATH /opt/conda/envs/nf-core-vep-${VEP_TAG}/bin:$PATH
 
 # Download Genome
 RUN vep_install \
@@ -27,4 +28,4 @@ RUN vep_install \
     --NO_BIOPERL --NO_HTSLIB --NO_TEST --NO_UPDATE
 
 # Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-vep-104.3 > nf-core-vep-104.3.yml
+RUN conda env export --name nf-core-vep-${VEP_TAG} > nf-core-vep-${VEP_TAG}.yml

modules/ensemblvep/build.sh

@@ -10,11 +10,12 @@ build_push() {
     VEP_TAG=$4
 
     docker build \
+        . \
         -t nfcore/vep:${VEP_TAG}.${GENOME} \
-        software/vep/. \
         --build-arg GENOME=${GENOME} \
         --build-arg SPECIES=${SPECIES} \
-        --build-arg VEP_VERSION=${VEP_VERSION}
+        --build-arg VEP_VERSION=${VEP_VERSION} \
+        --build-arg VEP_TAG=${VEP_TAG}
 
     docker push nfcore/vep:${VEP_TAG}.${GENOME}
 }

modules/ensemblvep/main.nf

@@ -13,6 +13,7 @@ process ENSEMBLVEP {
     val   species
     val   cache_version
     path  cache
+    path  extra_files
 
     output:
     tuple val(meta), path("*.ann.vcf"), emit: vcf

modules/ensemblvep/meta.yml

@@ -10,17 +10,6 @@ tools:
       homepage: https://www.ensembl.org/info/docs/tools/vep/index.html
       documentation: https://www.ensembl.org/info/docs/tools/vep/script/index.html
       licence: ["Apache-2.0"]
-params:
-  - use_cache:
-      type: boolean
-      description: |
-        Enable the usage of containers with cache
-        Does not work with conda
-  - vep_tag:
-      type: value
-      description: |
-        Specify the tag for the container
-        https://hub.docker.com/r/nfcore/vep/tags
 input:
   - meta:
       type: map
@@ -47,6 +36,10 @@ input:
       type: file
       description: |
         path to VEP cache (optional)
+  - extra_files:
+      type: tuple
+      description: |
+        path to file(s) needed for plugins  (optional)
 output:
   - vcf:
       type: file

modules/happy/happy/main.nf

@@ -0,0 +1,42 @@
+def VERSION = '0.3.14'
+
+process HAPPY_HAPPY {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::hap.py=0.3.14" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/hap.py:0.3.14--py27h5c5a3ab_0':
+        'quay.io/biocontainers/hap.py:0.3.14--py27h5c5a3ab_0' }"
+
+    input:
+    tuple val(meta), path(truth_vcf), path(query_vcf), path(bed)
+    tuple path(fasta), path(fasta_fai)
+
+    output:
+    tuple val(meta), path('*.csv'), path('*.json')  , emit: metrics
+    path "versions.yml"                             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    """
+    hap.py \\
+        $truth_vcf \\
+        $query_vcf \\
+        $args \\
+        --reference $fasta \\
+        --threads $task.cpus \\
+        -R $bed \\
+        -o $prefix
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        hap.py: $VERSION
+    END_VERSIONS
+    """
+}

modules/happy/happy/meta.yml

@@ -0,0 +1,67 @@
+name: "happy_happy"
+description: Hap.py is a tool to compare diploid genotypes at haplotype level. Rather than comparing VCF records row by row, hap.py will generate and match alternate sequences in a superlocus. A superlocus is a small region of the genome (sized between 1 and around 1000 bp) that contains one or more variants.
+keywords:
+  - happy
+  - benchmark
+  - haplotype
+tools:
+  - "happy":
+      description: "Haplotype VCF comparison tools"
+      homepage: "https://www.illumina.com/products/by-type/informatics-products/basespace-sequence-hub/apps/hap-py-benchmarking.html"
+      documentation: "https://github.com/Illumina/hap.py"
+      tool_dev_url: "https://github.com/Illumina/hap.py"
+      doi: ""
+      licence: "['BSD-2-clause']"
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - truth_vcf:
+      type: file
+      description: gold standard VCF file
+      pattern: "*.{vcf,vcf.gz}"
+  - query_vcf:
+      type: file
+      description: VCF/GVCF file to query
+      pattern: "*.{vcf,vcf.gz}"
+  - bed:
+      type: file
+      description: BED file
+      pattern: "*.bed"
+  - fasta:
+      type: file
+      description: FASTA file of the reference genome
+      pattern: "*.{fa,fasta}"
+  - fasta_fai:
+      type: file
+      description: The index of the reference FASTA
+      pattern: "*.fai"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - summary:
+      type: file
+      description: A CSV file containing the summary of the benchmarking
+      pattern: "*.summary.csv"
+  - extended:
+      type: file
+      description: A CSV file containing extended info of the benchmarking
+      pattern: "*.extended.csv"
+  - runinfo:
+      type: file
+      description: A JSON file containing the run info
+      pattern: "*.runinfo.json"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@nvnieuwk"

modules/happy/prepy/main.nf

@@ -0,0 +1,41 @@
+def VERSION = '0.3.14'
+
+process HAPPY_PREPY {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::hap.py=0.3.14" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/hap.py:0.3.14--py27h5c5a3ab_0':
+        'quay.io/biocontainers/hap.py:0.3.14--py27h5c5a3ab_0' }"
+
+    input:
+    tuple val(meta), path(vcf), path(bed)
+    tuple path(fasta), path(fasta_fai)
+
+    output:
+    tuple val(meta), path('*.vcf.gz')  , emit: preprocessed_vcf
+    path "versions.yml"                , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    """
+    pre.py \\
+        $args \\
+        -R $bed \\
+        --reference $fasta \\
+        --threads $task.cpus \\
+        $vcf \\
+        ${prefix}.vcf.gz
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        pre.py: $VERSION
+    END_VERSIONS
+    """
+}

modules/happy/prepy/meta.yml

@@ -0,0 +1,55 @@
+name: "happy_prepy"
+description: Pre.py is a preprocessing tool made to preprocess VCF files for Hap.py
+keywords:
+  - happy
+  - benchmark
+  - haplotype
+tools:
+  - "happy":
+      description: "Haplotype VCF comparison tools"
+      homepage: "https://www.illumina.com/products/by-type/informatics-products/basespace-sequence-hub/apps/hap-py-benchmarking.html"
+      documentation: "https://github.com/Illumina/hap.py"
+      tool_dev_url: "https://github.com/Illumina/hap.py"
+      doi: ""
+      licence: "['BSD-2-clause']"
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - vcf:
+      type: file
+      description: VCF file to preprocess
+      pattern: "*.{vcf,vcf.gz}"
+  - bed:
+      type: file
+      description: BED file
+      pattern: "*.bed"
+  - fasta:
+      type: file
+      description: FASTA file of the reference genome
+      pattern: "*.{fa,fasta}"
+  - fasta_fai:
+      type: file
+      description: The index of the reference FASTA
+      pattern: "*.fai"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - vcf:
+      type: file
+      description: A preprocessed VCF file
+      pattern: "*.vcf.gz"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+authors:
+  - "@nvnieuwk"

modules/krona/kronadb/main.nf

@@ -18,7 +18,9 @@ process KRONA_KRONADB {
     script:
     def args = task.ext.args ?: ''
     """
-    ktUpdateTaxonomy.sh taxonomy
+    ktUpdateTaxonomy.sh \\
+        $args \\
+        taxonomy/
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

modules/krona/ktimporttaxonomy/main.nf

@@ -23,7 +23,10 @@ process KRONA_KTIMPORTTAXONOMY {
     script:
     def args = task.ext.args ?: ''
     """
-    ktImportTaxonomy "$report" -tax taxonomy
+    ktImportTaxonomy \\
+        $args \\
+        -tax taxonomy/ \\
+        "$report"
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

modules/krona/ktimporttaxonomy/meta.yml

@@ -23,8 +23,11 @@ input:
         Groovy Map containing sample information
         e.g. [ id:'test']
   - database:
-      type: path
-      description: "Path to the taxonomy database downloaded by krona/kronadb"
+      type: file
+      description: |
+        Path to the taxonomy database .tab file downloaded by krona/ktUpdateTaxonomy
+        The file will be saved under a folder named "taxonomy" as "taxonomy/taxonomy.tab".
+        The parent folder will be passed as argument to ktImportTaxonomy.
   - report:
       type: file
       description: "A tab-delimited file with taxonomy IDs and (optionally) query IDs, magnitudes, and scores.  Query IDs are taken from column 1, taxonomy IDs from column 2, and scores from column 3.  Lines beginning with # will be ignored."

modules/krona/ktupdatetaxonomy/main.nf

@@ -0,0 +1,30 @@
+def VERSION='2.7.1' // Version information not provided by tool on CLI
+
+process KRONA_KTUPDATETAXONOMY {
+    label 'process_low'
+
+    conda (params.enable_conda ? "bioconda::krona=2.7.1" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/krona:2.7.1--pl526_5' :
+        'quay.io/biocontainers/krona:2.7.1--pl526_5' }"
+
+    output:
+    path 'taxonomy/taxonomy.tab', emit: db
+    path "versions.yml"         , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    """
+    ktUpdateTaxonomy.sh \\
+        $args \\
+        taxonomy/
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        krona: $VERSION
+    END_VERSIONS
+    """
+}

modules/krona/ktupdatetaxonomy/meta.yml

@@ -0,0 +1,31 @@
+name: krona_ktupdatetaxonomy
+description: KronaTools Update Taxonomy downloads a taxonomy database
+keywords:
+  - database
+  - taxonomy
+  - krona
+  - visualisation
+tools:
+  - krona:
+      description: Krona Tools is a set of scripts to create Krona charts from several Bioinformatics tools as well as from text and XML files.
+      homepage: https://github.com/marbl/Krona/wiki/KronaTools
+      documentation: https://github.com/marbl/Krona/wiki/Installing
+      tool_dev_url:
+      doi: https://doi.org/10.1186/1471-2105-12-385
+      licence:
+
+input:
+  - none: There is no input. This module downloads a pre-built taxonomy database for use with Krona Tools.
+
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - db:
+      type: file
+      description: A TAB separated file that contains a taxonomy database.
+      pattern: "*.{tab}"
+
+authors:
+  - "@mjakobs"

modules/md5sum/main.nf

@@ -0,0 +1,35 @@
+process MD5SUM {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda (params.enable_conda ? "conda-forge::coreutils=9.1" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
+        'ubuntu:20.04' }"
+
+    input:
+    tuple val(meta), path(file)
+
+    output:
+    tuple val(meta), path("*.md5"), emit: checksum
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    """
+    md5sum \\
+        $args \\
+        ${file} \\
+        > ${file}.md5
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        md5sum: \$(echo \$(md5sum --version 2>&1 | head -n 1| sed 's/^.*) //;' ))
+    END_VERSIONS
+    """
+}

modules/md5sum/meta.yml

@@ -0,0 +1,39 @@
+name: "md5sum"
+description: Create an MD5 (128-bit) checksum
+keywords:
+  - checksum
+tools:
+  - "md5sum":
+      description: Create an MD5 (128-bit) checksum
+      homepage: "https://www.gnu.org"
+      documentation: "https://man7.org/linux/man-pages/man1/md5sum.1.html"
+      licence: GPLv3+
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - file:
+      type: file
+      description: Any file
+      pattern: "*.*"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - checksum:
+      type: file
+      description: File containing checksum
+      pattern: "*.md5"
+
+authors:
+  - "@matthdsm"

modules/motus/downloaddb/main.nf

@@ -0,0 +1,39 @@
+process MOTUS_DOWNLOADDB {
+    label 'process_low'
+
+    conda (params.enable_conda ? "bioconda::motus=3.0.1" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/motus:3.0.1--pyhdfd78af_0':
+        'quay.io/biocontainers/motus:3.0.1--pyhdfd78af_0' }"
+
+    input:
+    path motus_downloaddb_script
+
+    output:
+    path "db_mOTU/"                , emit: db
+    path "versions.yml"            , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args     = task.ext.args ?: ''
+    def software = "${motus_downloaddb_script.simpleName}_copy.py"
+    """
+    ## must copy script file to working directory,
+    ## otherwise the reference_db will be download to bin folder
+    ## other than current directory
+    cp $motus_downloaddb_script ${software}
+    python ${software} \\
+        $args \\
+        -t $task.cpus
+
+    ## mOTUs version number is not available from command line.
+    ## mOTUs save the version number in index database folder.
+    ## mOTUs will check the database version is same version as exec version.
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        mOTUs: \$(grep motus db_mOTU/db_mOTU_versions | sed 's/motus\\t//g')
+    END_VERSIONS
+    """
+}

modules/motus/downloaddb/meta.yml

@@ -0,0 +1,39 @@
+name: "motus_downloaddb"
+description: Download the mOTUs database
+keywords:
+  - classify
+  - metagenomics
+  - fastq
+  - taxonomic profiling
+  - database
+  - download
+tools:
+  - "motus":
+      description: "The mOTU profiler is a computational tool that estimates relative taxonomic abundance of known and currently unknown microbial community members using metagenomic shotgun sequencing data."
+      homepage: "None"
+      documentation: "https://github.com/motu-tool/mOTUs/wiki"
+      tool_dev_url: "https://github.com/motu-tool/mOTUs"
+      doi: "10.1038/s41467-019-08844-4"
+      licence: "['GPL v3']"
+
+input:
+  - motus_downloaddb:
+      type: directory
+      description: |
+        The mOTUs downloadDB script source file.
+        It is the source file installed or
+        remote source in github such as https://raw.githubusercontent.com/motu-tool/mOTUs/master/motus/downloadDB.py
+      pattern: "downloadDB.py"
+
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - db:
+      type: directory
+      description: The mOTUs database directory
+      pattern: "db_mOTU"
+
+authors:
+  - "@jianhong"

modules/samtools/bam2fq/main.nf

@@ -45,7 +45,7 @@ process SAMTOOLS_BAM2FQ {
             bam2fq \\
             $args \\
             -@ $task.cpus \\
-            $inputbam >${prefix}_interleaved.fq.gz
+            $inputbam | gzip --no-name > ${prefix}_interleaved.fq.gz
 
         cat <<-END_VERSIONS > versions.yml
         "${task.process}":

modules/shasum/main.nf

@@ -0,0 +1,35 @@
+process SHASUM {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda (params.enable_conda ? "conda-forge::coreutils=9.1" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
+        'ubuntu:20.04' }"
+
+    input:
+    tuple val(meta), path(file)
+
+    output:
+    tuple val(meta), path("*.sha256"), emit: checksum
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    """
+    sha256sum \\
+        $args \\
+        ${file} \\
+        > ${file}.sha256
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        sha256sum: \$(echo \$(sha256sum --version 2>&1 | head -n 1| sed 's/^.*) //;' ))
+    END_VERSIONS
+    """
+}

modules/shasum/meta.yml

@@ -0,0 +1,40 @@
+name: "shasum"
+description: Print SHA256 (256-bit) checksums.
+keywords:
+  - checksum
+  - sha256
+tools:
+  - "md5sum":
+      description: Create an SHA256 (256-bit) checksum.
+      homepage: "https://www.gnu.org"
+      documentation: "https://linux.die.net/man/1/shasum"
+      licence: GPLv3+
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - file:
+      type: file
+      description: Any file
+      pattern: "*.*"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - checksum:
+      type: file
+      description: File containing checksum
+      pattern: "*.sha256"
+
+authors:
+  - "@matthdsm"

modules/snpeff/Dockerfile

@@ -8,15 +8,16 @@ LABEL \
 COPY environment.yml /
 RUN conda env create -f /environment.yml && conda clean -a
 
-# Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-snpeff-5.0/bin:$PATH
-
 # Setup default ARG variables
 ARG GENOME=GRCh38
 ARG SNPEFF_CACHE_VERSION=99
+ARG SNPEFF_TAG=99
+
+# Add conda installation dir to PATH (instead of doing 'conda activate')
+ENV PATH /opt/conda/envs/nf-core-snpeff-${SNPEFF_TAG}/bin:$PATH
 
 # Download Genome
 RUN snpEff download -v ${GENOME}.${SNPEFF_CACHE_VERSION}
 
 # Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-snpeff-5.0 > nf-core-snpeff-5.0.yml
+RUN conda env export --name nf-core-snpeff-${SNPEFF_TAG} > nf-core-snpeff-${SNPEFF_TAG}.yml

modules/snpeff/build.sh

@@ -9,10 +9,11 @@ build_push() {
     SNPEFF_TAG=$3
 
     docker build \
+        . \
         -t nfcore/snpeff:${SNPEFF_TAG}.${GENOME} \
-        software/snpeff/. \
         --build-arg GENOME=${GENOME} \
-        --build-arg SNPEFF_CACHE_VERSION=${SNPEFF_CACHE_VERSION}
+        --build-arg SNPEFF_CACHE_VERSION=${SNPEFF_CACHE_VERSION} \
+        --build-arg SNPEFF_TAG=${SNPEFF_TAG}
 
     docker push nfcore/snpeff:${SNPEFF_TAG}.${GENOME}
 }

modules/snpeff/meta.yml

@@ -10,18 +10,6 @@ tools:
       homepage: https://pcingola.github.io/SnpEff/
       documentation: https://pcingola.github.io/SnpEff/se_introduction/
       licence: ["MIT"]
-params:
-  - use_cache:
-      type: boolean
-      description: |
-        boolean to enable the usage of containers with cache
-        Enable the usage of containers with cache
-        Does not work with conda
-  - snpeff_tag:
-      type: value
-      description: |
-        Specify the tag for the container
-        https://hub.docker.com/r/nfcore/snpeff/tags
 input:
   - meta:
       type: map

modules/svdb/merge/main.nf

@@ -2,10 +2,10 @@ process SVDB_MERGE {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::svdb=2.6.0" : null)
+    conda (params.enable_conda ? "bioconda::svdb=2.6.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/svdb:2.6.0--py39h5371cbf_0':
-        'quay.io/biocontainers/svdb:2.6.0--py39h5371cbf_0' }"
+        'https://depot.galaxyproject.org/singularity/svdb:2.6.1--py39h5371cbf_0':
+        'quay.io/biocontainers/svdb:2.6.1--py39h5371cbf_0' }"
 
     input:
     tuple val(meta), path(vcfs)

modules/svdb/query/main.nf

@@ -2,10 +2,10 @@ process SVDB_QUERY {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::svdb=2.6.0" : null)
+    conda (params.enable_conda ? "bioconda::svdb=2.6.1" : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/svdb:2.6.0--py39h5371cbf_0':
-        'quay.io/biocontainers/svdb:2.6.0--py39h5371cbf_0' }"
+        'https://depot.galaxyproject.org/singularity/svdb:2.6.1--py39h5371cbf_0':
+        'quay.io/biocontainers/svdb:2.6.1--py39h5371cbf_0' }"
 
     input:
     tuple val(meta), path(vcf)

subworkflows/nf-core/annotation/ensemblvep/main.nf

@@ -0,0 +1,31 @@
+//
+// Run VEP to annotate VCF files
+//
+
+include { ENSEMBLVEP       } from '../../../../modules/ensemblvep/main'
+include { TABIX_BGZIPTABIX } from '../../../../modules/tabix/bgziptabix/main'
+
+workflow ANNOTATION_ENSEMBLVEP {
+    take:
+    vcf               // channel: [ val(meta), vcf ]
+    vep_genome        //   value: genome to use
+    vep_species       //   value: species to use
+    vep_cache_version //   value: cache version to use
+    vep_cache         //    path: /path/to/vep/cache (optionnal)
+    vep_extra_files   // channel: [ file1, file2...] (optionnal)
+
+    main:
+    ch_versions = Channel.empty()
+
+    ENSEMBLVEP(vcf, vep_genome, vep_species, vep_cache_version, vep_cache, vep_extra_files)
+    TABIX_BGZIPTABIX(ENSEMBLVEP.out.vcf)
+
+    // Gather versions of all tools used
+    ch_versions = ch_versions.mix(ENSEMBLVEP.out.versions.first())
+    ch_versions = ch_versions.mix(TABIX_BGZIPTABIX.out.versions.first())
+
+    emit:
+    vcf_tbi  = TABIX_BGZIPTABIX.out.gz_tbi // channel: [ val(meta), vcf.gz, vcf.gz.tbi ]
+    reports  = ENSEMBLVEP.out.report       //    path: *.html
+    versions = ch_versions                 //    path: versions.yml
+}

subworkflows/nf-core/annotation/ensemblvep/meta.yml

@@ -0,0 +1,49 @@
+name: annotation_ensemblvep
+description: |
+  Perform annotation with ensemblvep and bgzip + tabix index the resulting VCF file
+keywords:
+  - ensemblvep
+modules:
+  - ensemblvep
+  - tabix/bgziptabix
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - vcf:
+      type: file
+      description: |
+        vcf to annotate
+  - genome:
+      type: value
+      description: |
+        which genome to annotate with
+  - species:
+      type: value
+      description: |
+        which species to annotate with
+  - cache_version:
+      type: value
+      description: |
+        which version of the cache to annotate with
+  - cache:
+      type: file
+      description: |
+        path to VEP cache (optional)
+  - extra_files:
+      type: tuple
+      description: |
+        path to file(s) needed for plugins  (optional)
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - vcf_tbi:
+      type: file
+      description: Compressed vcf file + tabix index
+      pattern: "[ *{.vcf.gz,vcf.gz.tbi} ]"
+authors:
+  - "@maxulysse"

subworkflows/nf-core/annotation/snpeff/main.nf

@@ -0,0 +1,28 @@
+//
+// Run SNPEFF to annotate VCF files
+//
+
+include { SNPEFF           } from '../../../../modules/snpeff/main'
+include { TABIX_BGZIPTABIX } from '../../../../modules/tabix/bgziptabix/main'
+
+workflow ANNOTATION_SNPEFF {
+    take:
+    vcf          // channel: [ val(meta), vcf ]
+    snpeff_db    //   value: db version to use
+    snpeff_cache //    path: /path/to/snpeff/cache (optionnal)
+
+    main:
+    ch_versions = Channel.empty()
+
+    SNPEFF(vcf, snpeff_db, snpeff_cache)
+    TABIX_BGZIPTABIX(SNPEFF.out.vcf)
+
+    // Gather versions of all tools used
+    ch_versions = ch_versions.mix(SNPEFF.out.versions.first())
+    ch_versions = ch_versions.mix(TABIX_BGZIPTABIX.out.versions.first())
+
+    emit:
+    vcf_tbi  = TABIX_BGZIPTABIX.out.gz_tbi // channel: [ val(meta), vcf.gz, vcf.gz.tbi ]
+    reports  = SNPEFF.out.report           //    path: *.html
+    versions = ch_versions                 //    path: versions.yml
+}

subworkflows/nf-core/annotation/snpeff/meta.yml

@@ -11,11 +11,19 @@ input:
       type: map
       description: |
         Groovy Map containing sample information
-        e.g. [ id:'test' ]
-  - input:
-      type: vcf
-      description: list containing one vcf file
-      pattern: "[ *.{vcf,vcf.gz} ]"
+        e.g. [ id:'test', single_end:false ]
+  - vcf:
+      type: file
+      description: |
+        vcf to annotate
+  - db:
+      type: value
+      description: |
+        which db to annotate with
+  - cache:
+      type: file
+      description: |
+        path to snpEff cache (optional)
 output:
   - versions:
       type: file

subworkflows/nf-core/annotation_ensemblvep/main.nf

@@ -1,26 +0,0 @@
-//
-// Run VEP to annotate VCF files
-//
-
-include { ENSEMBLVEP                                } from '../../../modules/ensemblvep/main'
-include { TABIX_BGZIPTABIX as ANNOTATION_BGZIPTABIX } from '../../../modules/tabix/bgziptabix/main'
-
-workflow ANNOTATION_ENSEMBLVEP {
-    take:
-    vcf               // channel: [ val(meta), vcf ]
-    vep_genome        //   value: which genome
-    vep_species       //   value: which species
-    vep_cache_version //   value: which cache version
-    vep_cache         //    path: path_to_vep_cache (optionnal)
-
-    main:
-    ENSEMBLVEP(vcf, vep_genome, vep_species, vep_cache_version, vep_cache)
-    ANNOTATION_BGZIPTABIX(ENSEMBLVEP.out.vcf)
-
-    ch_versions = ENSEMBLVEP.out.versions.first().mix(ANNOTATION_BGZIPTABIX.out.versions.first())
-
-    emit:
-    vcf_tbi  = ANNOTATION_BGZIPTABIX.out.gz_tbi // channel: [ val(meta), vcf.gz, vcf.gz.tbi ]
-    reports  = ENSEMBLVEP.out.report            //    path: *.html
-    versions = ch_versions                      //    path: versions.yml
-}

subworkflows/nf-core/annotation_ensemblvep/meta.yml

@@ -1,29 +0,0 @@
-name: annotation_ensemblvep
-description: |
-  Perform annotation with ensemblvep and bgzip + tabix index the resulting VCF file
-keywords:
-  - ensemblvep
-modules:
-  - ensemblvep
-  - tabix/bgziptabix
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test' ]
-  - input:
-      type: vcf
-      description: list containing one vcf file
-      pattern: "[ *.{vcf,vcf.gz} ]"
-output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - vcf_tbi:
-      type: file
-      description: Compressed vcf file + tabix index
-      pattern: "[ *{.vcf.gz,vcf.gz.tbi} ]"
-authors:
-  - "@maxulysse"

subworkflows/nf-core/annotation_snpeff/main.nf

@@ -1,23 +0,0 @@
-//
-// Run SNPEFF to annotate VCF files
-//
-
-include { SNPEFF                                    } from '../../../modules/snpeff/main'
-include { TABIX_BGZIPTABIX as ANNOTATION_BGZIPTABIX } from '../../../modules/tabix/bgziptabix/main'
-
-workflow ANNOTATION_SNPEFF {
-    take:
-    vcf            // channel: [ val(meta), vcf ]
-    snpeff_db      //   value: version of db to use
-    snpeff_cache   //    path: path_to_snpeff_cache (optionnal)
-
-    main:
-    SNPEFF(vcf, snpeff_db, snpeff_cache)
-    ANNOTATION_BGZIPTABIX(SNPEFF.out.vcf)
-    ch_versions = SNPEFF.out.versions.first().mix(ANNOTATION_BGZIPTABIX.out.versions.first())
-
-    emit:
-    vcf_tbi  = ANNOTATION_BGZIPTABIX.out.gz_tbi // channel: [ val(meta), vcf.gz, vcf.gz.tbi ]
-    reports  = SNPEFF.out.report                //    path: *.html
-    versions = ch_versions                      //    path: versions.yml
-}

tests/config/pytest_modules.yml

@@ -891,6 +891,14 @@ hamronization/summarize:
   - modules/hamronization/summarize/**
   - tests/modules/hamronization/summarize/**
 
+happy/happy:
+  - modules/happy/happy/**
+  - tests/modules/happy/happy/**
+
+happy/prepy:
+  - modules/happy/prepy/**
+  - tests/modules/happy/prepy/**
+
 hicap:
   - modules/hicap/**
   - tests/modules/hicap/**
@@ -1058,6 +1066,10 @@ krona/ktimporttext:
   - modules/krona/ktimporttext/**
   - tests/modules/krona/ktimporttext/**
 
+krona/ktupdatetaxonomy:
+  - modules/krona/ktupdatetaxonomy/**
+  - tests/modules/krona/ktupdatetaxonomy/**
+
 last/dotplot:
   - modules/last/dotplot/**
   - tests/modules/last/dotplot/**
@@ -1178,6 +1190,10 @@ maxbin2:
   - modules/maxbin2/**
   - tests/modules/maxbin2/**
 
+md5sum:
+  - modules/md5sum/**
+  - tests/modules/md5sum/**
+
 medaka:
   - modules/medaka/**
   - tests/modules/medaka/**
@@ -1242,6 +1258,10 @@ mosdepth:
   - modules/mosdepth/**
   - tests/modules/mosdepth/**
 
+motus/downloaddb:
+  - modules/motus/downloaddb/**
+  - tests/modules/motus/downloaddb/**
+
 msisensor/msi:
   - modules/msisensor/msi/**
   - tests/modules/msisensor/msi/**
@@ -1719,6 +1739,10 @@ seqwish/induce:
   - modules/seqwish/induce/**
   - tests/modules/seqwish/induce/**
 
+shasum:
+  - modules/shasum/**
+  - tests/modules/shasum/**
+
 shigatyper:
   - modules/shigatyper/**
   - tests/modules/shigatyper/**

tests/config/test_data.config

@@ -111,7 +111,9 @@ params {
                 test_sequencing_summary                        = "${test_data_dir}/genomics/sarscov2/nanopore/sequencing_summary/test.sequencing_summary.txt"
             }
             'metagenome' {
+                classified_reads_assignment                    = "${test_data_dir}/genomics/sarscov2/metagenome/test_1.kraken2.reads.txt"
                 kraken_report                                  = "${test_data_dir}/genomics/sarscov2/metagenome/test_1.kraken2.report.txt"
+                krona_taxonomy                                 = "${test_data_dir}/genomics/sarscov2/metagenome/krona_taxonomy.tab"
             }
         }
         'homo_sapiens' {

tests/modules/bowtie2/align/nextflow.config

@@ -1,5 +1,16 @@
+params {
+    force_large_index = false
+}
+
 process {
 
     publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
-
+}
+
+if (params.force_large_index) {
+    process {
+        withName: BOWTIE2_BUILD {
+            ext.args = '--large-index'
+        }
+    }
 }

tests/modules/bowtie2/align/test.yml

@@ -39,3 +39,45 @@
       md5sum: 52be6950579598a990570fbcf5372184
     - path: ./output/bowtie2/bowtie2/genome.rev.2.bt2
       md5sum: e3b4ef343dea4dd571642010a7d09597
+
+- name: bowtie2 align single-end large-index
+  command: nextflow run ./tests/modules/bowtie2/align -entry test_bowtie2_align_single_end -c ./tests/config/nextflow.config -c ./tests/modules/bowtie2/align/nextflow.config --force_large_index
+  tags:
+    - bowtie2
+    - bowtie2/align
+  files:
+    - path: ./output/bowtie2/test.bam
+    - path: ./output/bowtie2/test.bowtie2.log
+    - path: ./output/bowtie2/bowtie2/genome.3.bt2l
+      md5sum: 8952b3e0b1ce9a7a5916f2e147180853
+    - path: ./output/bowtie2/bowtie2/genome.2.bt2l
+      md5sum: 22c284084784a0720989595e0c9461fd
+    - path: ./output/bowtie2/bowtie2/genome.1.bt2l
+      md5sum: 07d811cd4e350d56267183d2ac7023a5
+    - path: ./output/bowtie2/bowtie2/genome.4.bt2l
+      md5sum: c25be5f8b0378abf7a58c8a880b87626
+    - path: ./output/bowtie2/bowtie2/genome.rev.1.bt2l
+      md5sum: fda48e35925fb24d1c0785f021981e25
+    - path: ./output/bowtie2/bowtie2/genome.rev.2.bt2l
+      md5sum: 802c26d32b970e1b105032b7ce7348b4
+
+- name: bowtie2 align paired-end large-index
+  command: nextflow run ./tests/modules/bowtie2/align -entry test_bowtie2_align_paired_end -c ./tests/config/nextflow.config -c ./tests/modules/bowtie2/align/nextflow.config --force_large_index
+  tags:
+    - bowtie2
+    - bowtie2/align
+  files:
+    - path: ./output/bowtie2/test.bam
+    - path: ./output/bowtie2/test.bowtie2.log
+    - path: ./output/bowtie2/bowtie2/genome.3.bt2l
+      md5sum: 8952b3e0b1ce9a7a5916f2e147180853
+    - path: ./output/bowtie2/bowtie2/genome.2.bt2l
+      md5sum: 22c284084784a0720989595e0c9461fd
+    - path: ./output/bowtie2/bowtie2/genome.1.bt2l
+      md5sum: 07d811cd4e350d56267183d2ac7023a5
+    - path: ./output/bowtie2/bowtie2/genome.4.bt2l
+      md5sum: c25be5f8b0378abf7a58c8a880b87626
+    - path: ./output/bowtie2/bowtie2/genome.rev.1.bt2l
+      md5sum: fda48e35925fb24d1c0785f021981e25
+    - path: ./output/bowtie2/bowtie2/genome.rev.2.bt2l
+      md5sum: 802c26d32b970e1b105032b7ce7348b4

tests/modules/cnvpytor/callcnvs/test.yml

@@ -4,10 +4,8 @@
     - cnvpytor
     - cnvpytor/callcnvs
   files:
-    - path: output/cnvpytor/test.tsv
-      md5sum: d41d8cd98f00b204e9800998ecf8427e
+    - path: output/cnvpytor/test.pytor
     - path: output/cnvpytor/versions.yml
-      md5sum: 0bea08a253fcb2ff0ff79b99df77b9fa
 
 - name: cnvpytor callcnvs test_cnvpytor_callcnvs stub
   command: nextflow run tests/modules/cnvpytor/callcnvs -entry test_cnvpytor_callcnvs -c tests/config/nextflow.config -stub-run
@@ -15,6 +13,5 @@
     - cnvpytor
     - cnvpytor/callcnvs
   files:
-    - path: output/cnvpytor/test.tsv
+    - path: output/cnvpytor/test.pytor
     - path: output/cnvpytor/versions.yml
-      md5sum: 0bea08a253fcb2ff0ff79b99df77b9fa

tests/modules/cnvpytor/histogram/test.yml

@@ -5,9 +5,7 @@
     - cnvpytor/histogram
   files:
     - path: output/cnvpytor/test.pytor
-      md5sum: aa03a8fa15b39f77816705a48e10312a
     - path: output/cnvpytor/versions.yml
-      md5sum: 0f4d75c4f3a3eb26c22616d12b0b78b2
 
 - name: cnvpytor histogram test_cnvpytor_histogram stub
   command: nextflow run tests/modules/cnvpytor/histogram -entry test_cnvpytor_histogram -c tests/config/nextflow.config -stub-run
@@ -17,4 +15,3 @@
   files:
     - path: output/cnvpytor/test.pytor
     - path: output/cnvpytor/versions.yml
-      md5sum: 0f4d75c4f3a3eb26c22616d12b0b78b2

tests/modules/cnvpytor/importreaddepth/test.yml

@@ -6,7 +6,6 @@
   files:
     - path: output/cnvpytor/test.pytor
     - path: output/cnvpytor/versions.yml
-      md5sum: 5834495324c08a37f3fd73ccdd881dc8
 
 - name: cnvpytor importreaddepth test_cnvpytor_importreaddepth stub
   command: nextflow run tests/modules/cnvpytor/importreaddepth -entry test_cnvpytor_importreaddepth -c tests/config/nextflow.config -stub-run
@@ -16,7 +15,6 @@
   files:
     - path: output/cnvpytor/test.pytor
     - path: output/cnvpytor/versions.yml
-      md5sum: 5834495324c08a37f3fd73ccdd881dc8
 
 - name: cnvpytor importreaddepth test_cnvpytor_importreaddepth_cram
   command: nextflow run tests/modules/cnvpytor/importreaddepth -entry test_cnvpytor_importreaddepth_cram -c tests/config/nextflow.config
@@ -26,7 +24,6 @@
   files:
     - path: output/cnvpytor/test.pytor
     - path: output/cnvpytor/versions.yml
-      md5sum: dfa0afb0982d985b96d1633f71ebb82a
 
 - name: cnvpytor importreaddepth test_cnvpytor_importreaddepth_cram stub
   command: nextflow run tests/modules/cnvpytor/importreaddepth -entry test_cnvpytor_importreaddepth_cram -c tests/config/nextflow.config -stub-run
@@ -36,4 +33,3 @@
   files:
     - path: output/cnvpytor/test.pytor
     - path: output/cnvpytor/versions.yml
-      md5sum: dfa0afb0982d985b96d1633f71ebb82a

tests/modules/cnvpytor/partition/test.yml

@@ -5,9 +5,7 @@
     - cnvpytor/partition
   files:
     - path: output/cnvpytor/test.pytor
-      md5sum: aa03a8fa15b39f77816705a48e10312a
     - path: output/cnvpytor/versions.yml
-      md5sum: 7fd6ec952a316463bcd324f176b46b64
 
 - name: cnvpytor partition test_cnvpytor_partition stub
   command: nextflow run tests/modules/cnvpytor/partition -entry test_cnvpytor_partition -c tests/config/nextflow.config -stub-run
@@ -17,4 +15,3 @@
   files:
     - path: output/cnvpytor/test.pytor
     - path: output/cnvpytor/versions.yml
-      md5sum: 7fd6ec952a316463bcd324f176b46b64

tests/modules/cnvpytor/view/main.nf

@@ -0,0 +1,42 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { CNVPYTOR_VIEW } from '../../../../modules/cnvpytor/view/main.nf'
+
+workflow test_cnvpytor_view {
+
+    input = [
+        [ id:'test'], // meta map
+        [file(params.test_data['homo_sapiens']['illumina']['test_pytor'], checkIfExists: true)]
+    ]
+
+    bin_sizes = "10000 100000"
+
+    CNVPYTOR_VIEW ( input, bin_sizes, [] )
+}
+
+workflow test_cnvpytor_view_tsvout {
+
+    input = [
+        [ id:'test'], // meta map
+        [file(params.test_data['homo_sapiens']['illumina']['test_pytor'], checkIfExists: true)]
+    ]
+
+    output_suffix = "tsv"
+
+    CNVPYTOR_VIEW ( input, [], output_suffix )
+}
+
+workflow test_cnvpytor_view_stub {
+
+    input = [
+        [ id:'test'], // meta map
+        [file(params.test_data['homo_sapiens']['illumina']['test_pytor'], checkIfExists: true)]
+    ]
+
+    bin_sizes = []
+    output_suffix = []
+
+    CNVPYTOR_VIEW ( input, bin_sizes, output_suffix )
+}

tests/modules/cnvpytor/view/nextflow.config

@@ -0,0 +1,7 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+    withName: CNVPYTOR_VIEW {
+        ext.args = '10000 100000'
+    }
+}

tests/modules/cnvpytor/view/test.yml

@@ -0,0 +1,27 @@
+- name: cnvpytor view test_cnvpytor_view
+  command: nextflow run tests/modules/cnvpytor/view -entry test_cnvpytor_view -c tests/config/nextflow.config
+  tags:
+    - cnvpytor
+    - cnvpytor/view
+  files:
+    - path: output/cnvpytor/test_10000.vcf
+    - path: output/cnvpytor/test_100000.vcf
+    - path: output/cnvpytor/versions.yml
+
+- name: cnvpytor view test_cnvpytor_view tsv
+  command: nextflow run tests/modules/cnvpytor/view -entry test_cnvpytor_view_tsvout -c tests/config/nextflow.config
+  tags:
+    - cnvpytor
+    - cnvpytor/view
+  files:
+    - path: output/cnvpytor/test_1000.tsv
+    - path: output/cnvpytor/versions.yml
+
+- name: cnvpytor view test_cnvpytor_view stub
+  command: nextflow run tests/modules/cnvpytor/view -entry test_cnvpytor_view_stub -c tests/config/nextflow.config  -stub-run
+  tags:
+    - cnvpytor
+    - cnvpytor/view
+  files:
+    - path: output/cnvpytor/test.vcf
+    - path: output/cnvpytor/versions.yml

tests/modules/ensemblvep/main.nf

@@ -10,5 +10,5 @@ workflow test_ensemblvep {
         file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true)
     ]
 
-    ENSEMBLVEP ( input, "WBcel235", "caenorhabditis_elegans", "104", [] )
+    ENSEMBLVEP ( input, "WBcel235", "caenorhabditis_elegans", "104", [], [] )
 }

tests/modules/happy/happy/main.nf

@@ -0,0 +1,39 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { HAPPY_HAPPY } from '../../../../modules/happy/happy/main.nf'
+
+workflow test_happy_vcf {
+    
+    input = [
+        [ id:'test' ], // meta map
+        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_vcf'], checkIfExists: true),
+        file(params.test_data['homo_sapiens']['illumina']['test_genome21_indels_vcf_gz'], checkIfExists: true),
+        file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true)        
+    ]
+
+    fasta = Channel.value([
+        file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true),
+        file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true)
+    ])
+
+    HAPPY_HAPPY ( input, fasta )
+}
+
+workflow test_happy_gvcf {
+    
+    input = [
+        [ id:'test' ], // meta map
+        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_vcf'], checkIfExists: true),
+        file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf'], checkIfExists: true),
+        file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true)        
+    ]
+
+    fasta = Channel.value([
+        file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true),
+        file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true)
+    ])
+
+    HAPPY_HAPPY ( input, fasta )
+}

tests/modules/happy/happy/nextflow.config

@@ -0,0 +1,5 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+    
+}

tests/modules/happy/happy/test.yml

@@ -0,0 +1,27 @@
+- name: happy happy test_happy_vcf
+  command: nextflow run tests/modules/happy/happy -entry test_happy_vcf -c tests/config/nextflow.config
+  tags:
+    - happy
+    - happy/happy
+  files:
+    - path: output/happy/test.extended.csv
+      md5sum: ef79c7c789ef4f146ca2e50dafaf22b3
+    - path: output/happy/test.runinfo.json
+    - path: output/happy/test.summary.csv
+      md5sum: f8aa5d36d3c48dede2f607fd565894ad
+    - path: output/happy/versions.yml
+      md5sum: 82243bf6dbdc71aa63211ee2a89f47f2
+
+- name: happy happy test_happy_gvcf
+  command: nextflow run tests/modules/happy/happy -entry test_happy_gvcf -c tests/config/nextflow.config
+  tags:
+    - happy
+    - happy/happy
+  files:
+    - path: output/happy/test.extended.csv
+      md5sum: 3d5c21b67a259a3f6dcb088d55b86cd3
+    - path: output/happy/test.runinfo.json
+    - path: output/happy/test.summary.csv
+      md5sum: 03044e9bb5a0c6f0947b7e910fc8a558
+    - path: output/happy/versions.yml
+      md5sum: 551fa216952d6f5de78e6e453b92aaab

tests/modules/happy/prepy/main.nf

@@ -0,0 +1,37 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { HAPPY_PREPY } from '../../../../modules/happy/prepy/main.nf'
+
+workflow test_happy_prepy_vcf {
+    
+    input = [
+        [ id:'test' ], // meta map
+        file(params.test_data['homo_sapiens']['illumina']['test_genome21_indels_vcf_gz'], checkIfExists: true),
+        file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true)        
+    ]
+
+    fasta = Channel.value([
+        file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true),
+        file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true)
+    ])
+
+    HAPPY_PREPY ( input, fasta )
+}
+
+workflow test_happy_prepy_gvcf {
+    
+    input = [
+        [ id:'test' ], // meta map
+        file(params.test_data['homo_sapiens']['illumina']['test_genome_vcf'], checkIfExists: true),
+        file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true)        
+    ]
+
+    fasta = Channel.value([
+        file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true),
+        file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true)
+    ])
+
+    HAPPY_PREPY ( input, fasta )
+}

tests/modules/happy/prepy/nextflow.config

@@ -0,0 +1,5 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+    
+}

tests/modules/happy/prepy/test.yml

@@ -0,0 +1,19 @@
+- name: happy prepy test_happy_prepy_vcf
+  command: nextflow run tests/modules/happy/prepy -entry test_happy_prepy_vcf -c tests/config/nextflow.config
+  tags:
+    - happy/prepy
+    - happy
+  files:
+    - path: output/happy/test.vcf.gz
+    - path: output/happy/versions.yml
+      md5sum: 814d20f1f29f23a3d21012748a5d6393
+
+- name: happy prepy test_happy_prepy_gvcf
+  command: nextflow run tests/modules/happy/prepy -entry test_happy_prepy_gvcf -c tests/config/nextflow.config
+  tags:
+    - happy/prepy
+    - happy
+  files:
+    - path: output/happy/test.vcf.gz
+    - path: output/happy/versions.yml
+      md5sum: 970a54de46e68ef6d5228a26eaa4c8e7

tests/modules/krona/ktimporttaxonomy/main.nf

@@ -2,15 +2,27 @@
 
 nextflow.enable.dsl = 2
 
-include { KRONA_KTIMPORTTAXONOMY } from '../../../../modules/krona/ktimporttaxonomy/main.nf'
+include { KRONA_KTIMPORTTAXONOMY as KRONA_KTIMPORTTAXONOMY_READS  } from '../../../../modules/krona/ktimporttaxonomy/main.nf'
+include { KRONA_KTIMPORTTAXONOMY as KRONA_KTIMPORTTAXONOMY_REPORT } from '../../../../modules/krona/ktimporttaxonomy/main.nf'
 
-workflow test_krona_ktimporttaxonomy {
+workflow test_krona_ktimporttaxonomy_reads {
 
     input = [
         [ id:'test', single_end:false ], // meta map
-        file(params.test_data['generic']['txt']['hello'], checkIfExists: true)
+        file(params.test_data['sarscov2']['metagenome']['classified_reads_assignment'], checkIfExists: true)
     ]
-    taxonomy = file(params.test_data['generic']['txt']['hello'], checkIfExists: true)
+    taxonomy = file(params.test_data['sarscov2']['metagenome']['krona_taxonomy'], checkIfExists: true)
 
-    KRONA_KTIMPORTTAXONOMY ( input, taxonomy )
+    KRONA_KTIMPORTTAXONOMY_READS ( input, taxonomy )
+}
+
+workflow test_krona_ktimporttaxonomy_report {
+
+    input = [
+        [ id:'test', single_end:false ], // meta map
+        file(params.test_data['sarscov2']['metagenome']['kraken_report'], checkIfExists: true)
+    ]
+    taxonomy = file(params.test_data['sarscov2']['metagenome']['krona_taxonomy'], checkIfExists: true)
+
+    KRONA_KTIMPORTTAXONOMY_REPORT ( input, taxonomy )
 }

tests/modules/krona/ktimporttaxonomy/nextflow.config

@@ -2,4 +2,12 @@ process {
 
     publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
 
+    withName: KRONA_KTIMPORTTAXONOMY_READS {
+        ext.args = '-t 3'
+    }
+
+    withName: KRONA_KTIMPORTTAXONOMY_REPORT {
+        ext.args = '-m 3 -t 5'
+    }
+
 }

tests/modules/krona/ktimporttaxonomy/test.yml

@@ -1,9 +1,23 @@
-- name: krona ktimporttaxonomy test_krona_ktimporttaxonomy
-  command: nextflow run ./tests/modules/krona/ktimporttaxonomy -entry test_krona_ktimporttaxonomy -c ./tests/config/nextflow.config -c ./tests/modules/krona/ktimporttaxonomy/nextflow.config
+- name: krona ktimporttaxonomy test_krona_ktimporttaxonomy_reads
+  command: nextflow run tests/modules/krona/ktimporttaxonomy -entry test_krona_ktimporttaxonomy_reads -c tests/config/nextflow.config
   tags:
-    - krona/ktimporttaxonomy
     - krona
+    - krona/ktimporttaxonomy
   files:
     - path: output/krona/taxonomy.krona.html
       contains:
         - "DOCTYPE html PUBLIC"
+    - path: output/krona/versions.yml
+      md5sum: 660a8c151191bf4c63bd96db2c7fe503
+
+- name: krona ktimporttaxonomy test_krona_ktimporttaxonomy_report
+  command: nextflow run tests/modules/krona/ktimporttaxonomy -entry test_krona_ktimporttaxonomy_report -c tests/config/nextflow.config
+  tags:
+    - krona
+    - krona/ktimporttaxonomy
+  files:
+    - path: output/krona/taxonomy.krona.html
+      contains:
+        - "DOCTYPE html PUBLIC"
+    - path: output/krona/versions.yml
+      md5sum: 8a593c16bb2d4132638fb0fc342fe2b7

tests/modules/krona/ktupdatetaxonomy/main.nf

@@ -0,0 +1,9 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { KRONA_KTUPDATETAXONOMY } from '../../../../modules/krona/ktupdatetaxonomy/main.nf'
+
+workflow test_krona_ktupdatetaxonomy {
+    KRONA_KTUPDATETAXONOMY ( )
+}

tests/modules/krona/ktupdatetaxonomy/nextflow.config

@@ -0,0 +1,5 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+
+}

tests/modules/krona/ktupdatetaxonomy/test.yml

@@ -0,0 +1,7 @@
+- name: krona ktupdatetaxonomy test_krona_ktupdatetaxonomy
+  command: nextflow run ./tests/modules/krona/ktupdatetaxonomy -entry test_krona_ktupdatetaxonomy -c ./tests/config/nextflow.config -c ./tests/modules/krona/ktupdatetaxonomy/nextflow.config
+  tags:
+    - krona
+    - krona/ktupdatetaxonomy
+  files:
+    - path: output/krona/taxonomy/taxonomy.tab

tests/modules/md5sum/main.nf

@@ -0,0 +1,15 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { MD5SUM } from '../../../modules/md5sum/main.nf'
+
+workflow test_md5sum {
+    
+    input = [
+        [ id:'test', single_end:false ], // meta map
+        file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true)
+    ]
+
+    MD5SUM ( input )
+}

tests/modules/md5sum/nextflow.config

@@ -0,0 +1,3 @@
+process {
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+}

tests/modules/md5sum/test.yml

@@ -0,0 +1,8 @@
+- name: md5sum test_md5sum
+  command: nextflow run tests/modules/md5sum -entry test_md5sum -c tests/config/nextflow.config
+  tags:
+    - md5sum
+  files:
+    - path: output/md5sum/test.paired_end.bam.md5
+      md5sum: 1163095be8fdfb2acb3cc6c027389c4b
+    - path: output/md5sum/versions.yml

tests/modules/motus/downloaddb/main.nf

@@ -0,0 +1,12 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { MOTUS_DOWNLOADDB } from '../../../../modules/motus/downloaddb/main.nf'
+
+workflow test_motus_downloaddb {
+
+    input = file('https://raw.githubusercontent.com/motu-tool/mOTUs/master/motus/downloadDB.py')
+
+    MOTUS_DOWNLOADDB ( input )
+}

tests/modules/motus/downloaddb/nextflow.config

@@ -0,0 +1,5 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+    
+}

tests/modules/motus/downloaddb/test.yml

@@ -0,0 +1,7 @@
+- name: motus downloaddb test_motus_downloaddb
+  command: nextflow run tests/modules/motus/downloaddb -entry test_motus_downloaddb -c tests/config/nextflow.config
+  tags:
+    - motus
+    - motus/downloaddb
+  files:
+    - path: output/motus/db_mOTU/db_mOTU_versions

tests/modules/samtools/bam2fq/test.yml

@@ -1,14 +1,15 @@
 - name: samtools bam2fq test_samtools_bam2fq_nosplit
-  command: nextflow run ./tests/modules/samtools/bam2fq -entry test_samtools_bam2fq_nosplit -c ./tests/config/nextflow.config -c ./tests/modules/samtools/bam2fq/nextflow.config
+  command: nextflow run tests/modules/samtools/bam2fq -entry test_samtools_bam2fq_nosplit -c tests/config/nextflow.config
   tags:
     - samtools/bam2fq
     - samtools
   files:
     - path: output/samtools/test_interleaved.fq.gz
-      md5sum: d733e66d29a4b366bf9df8c42f845256
+    - path: output/samtools/versions.yml
+      md5sum: 4973eac1b6a8f090d5fcd4456d65a894
 
 - name: samtools bam2fq test_samtools_bam2fq_withsplit
-  command: nextflow run ./tests/modules/samtools/bam2fq -entry test_samtools_bam2fq_withsplit -c ./tests/config/nextflow.config -c ./tests/modules/samtools/bam2fq/nextflow.config
+  command: nextflow run tests/modules/samtools/bam2fq -entry test_samtools_bam2fq_withsplit -c tests/config/nextflow.config
   tags:
     - samtools/bam2fq
     - samtools
@@ -21,3 +22,5 @@
       md5sum: 709872fc2910431b1e8b7074bfe38c67
     - path: output/samtools/test_singleton.fq.gz
       md5sum: 709872fc2910431b1e8b7074bfe38c67
+    - path: output/samtools/versions.yml
+      md5sum: e92d21bbcda2fed7cb438d95c51edff0

tests/modules/shasum/main.nf

@@ -0,0 +1,15 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { SHASUM } from '../../../modules/shasum/main.nf'
+
+workflow test_shasum {
+    
+    input = [
+        [ id:'test', single_end:false ], // meta map
+        file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true)
+    ]
+
+    SHASUM ( input )
+}

tests/modules/shasum/nextflow.config

@@ -0,0 +1,5 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+    
+}

tests/modules/shasum/test.yml

@@ -0,0 +1,8 @@
+- name: shasum test_shasum
+  command: nextflow run tests/modules/shasum -entry test_shasum -c tests/config/nextflow.config
+  tags:
+    - shasum
+  files:
+    - path: output/shasum/test.paired_end.bam.sha256
+      md5sum: 138a19e100f09fc975ea1b717da9b6dd
+    - path: output/shasum/versions.yml

tests/subworkflows/nf-core/annotation/ensemblvep/main.nf

@@ -2,7 +2,7 @@
 
 nextflow.enable.dsl = 2
 
-include { ANNOTATION_ENSEMBLVEP } from '../../../../subworkflows/nf-core/annotation_ensemblvep/main'
+include { ANNOTATION_ENSEMBLVEP } from '../../../../../subworkflows/nf-core/annotation/ensemblvep/main'
 
 workflow annotation_ensemblvep {
     input = [
@@ -10,5 +10,5 @@ workflow annotation_ensemblvep {
         file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true)
     ]
 
-    ANNOTATION_ENSEMBLVEP ( input, "WBcel235", "caenorhabditis_elegans", "104", [] )
+    ANNOTATION_ENSEMBLVEP ( input, "WBcel235", "caenorhabditis_elegans", "104", [], [] )
 }

tests/subworkflows/nf-core/annotation/ensemblvep/nextflow.config

@@ -7,7 +7,7 @@ process {
         publishDir = [ enabled: false ]
     }
 
-    withName: ANNOTATION_BGZIPTABIX {
+    withName: TABIX_BGZIPTABIX {
         ext.prefix = { "${meta.id}_VEP.ann.vcf" }
     }
 

tests/subworkflows/nf-core/annotation/snpeff/main.nf

@@ -2,7 +2,7 @@
 
 nextflow.enable.dsl = 2
 
-include { ANNOTATION_SNPEFF } from '../../../../subworkflows/nf-core/annotation_snpeff/main'
+include { ANNOTATION_SNPEFF } from '../../../../../subworkflows/nf-core/annotation_snpeff/main'
 
 workflow annotation_snpeff {
     input = [

tests/subworkflows/nf-core/annotation/snpeff/nextflow.config

@@ -7,7 +7,7 @@ process {
         publishDir = [ enabled: false ]
     }
 
-    withName: ANNOTATION_BGZIPTABIX {
+    withName: TABIX_BGZIPTABIX {
         ext.prefix = { "${meta.id}_snpEff.ann.vcf" }
     }