Add ivar consensus

This commit is contained in:
Anders Goncalves da Silva 2021-02-09 10:26:35 -08:00
parent 1baca83f00
commit 8f546a1f95
6 changed files with 137 additions and 0 deletions

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/*
* -----------------------------------------------------
* Utility functions used in nf-core DSL2 module files
* -----------------------------------------------------
*/
/*
* Extract name of software tool from process name using $task.process
*/
def getSoftwareName(task_process) {
return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
}
/*
* Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
*/
def initOptions(Map args) {
def Map options = [:]
options.args = args.args ?: ''
options.args2 = args.args2 ?: ''
options.publish_by_id = args.publish_by_id ?: false
options.publish_dir = args.publish_dir ?: ''
options.publish_files = args.publish_files
options.suffix = args.suffix ?: ''
return options
}
/*
* Tidy up and join elements of a list to return a path string
*/
def getPathFromList(path_list) {
def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries
paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
return paths.join('/')
}
/*
* Function to save/publish module results
*/
def saveFiles(Map args) {
if (!args.filename.endsWith('.version.txt')) {
def ioptions = initOptions(args.options)
def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
if (ioptions.publish_by_id) {
path_list.add(args.publish_id)
}
if (ioptions.publish_files instanceof Map) {
for (ext in ioptions.publish_files) {
if (args.filename.endsWith(ext.key)) {
def ext_list = path_list.collect()
ext_list.add(ext.value)
return "${getPathFromList(ext_list)}/$args.filename"
}
}
} else if (ioptions.publish_files == null) {
return "${getPathFromList(path_list)}/$args.filename"
}
}
}

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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
params.options = [:]
def options = initOptions(params.options)
process IVAR_CONSENSUS {
tag "$meta.id"
label 'process_medium'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
conda (params.enable_conda ? "bioconda::ivar=1.3.1" : null)
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
container "https://depot.galaxyproject.org/singularity/ivar:1.3.1--h089eab3_0"
} else {
container "quay.io/biocontainers/ivar:1.3.1--h089eab3_0"
}
input:
tuple val(meta), path(bam)
output:
tuple val(meta), path("*.fa") , emit: cns_seq
tuple val(meta), path("*.qual.txt"), emit: cns_qual
tuple val(meta), path("*_mpileup.txt"), emit: mpileup
path "*.version.txt" , emit: version
script:
def software = getSoftwareName(task.process)
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
"""
samtools mpileup \\
-aa -A -d 0 -Q 0 \\
$options.args2 \\
$bam | \\
tee ${prefix}_mpileup.txt | \\
ivar consensus \\
$options.args \\
-p $prefix
ivar version | head -n1 2>&1 | sed 's/^.*iVar version //g' > ${software}.version.txt
"""
}

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#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
params.publish_dir_mode = 'link'
params.enable_conda = false
include { IVAR_CONSENSUS } from '../../../../software/ivar/consensus/main.nf' addParams([:])
workflow test_ivar_consensus {
bam = file("${launchDir}/tests/data/bam/test-sc2-artic-v3.bam", checkIfExists: true)
def input = []
input = [ [ id:'test'],
file("${launchDir}/tests/data/bam/test-sc2-artic-v3-sorted-trimmed.bam", checkIfExists: true) ]
main:
IVAR_CONSENSUS ( input )
}

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- name: Run ivar consensus test workflow
command: nextflow run ./tests/software/ivar/consensus -profile docker -entry test_ivar_consensus -c tests/config/nextflow.config
tags:
- ivar
- ivar_consensus
files:
- path: output/ivar/test.fa
md5sum: d791e777b669bd40f6074b5566722a47
- path: output/ivar/test.qual.txt
md5sum: 723126475ec74adfd876e2b47ffe35a8
- path: output/ivar/test_mpileup.txt
md5sum: d1c0ef3978bec1c09c549f884ed5b76a