diff --git a/tests/software/bowtie2/test.yml b/tests/software/bowtie2/test.yml
index 85bb01c8..a3c6f31d 100644
--- a/tests/software/bowtie2/test.yml
+++ b/tests/software/bowtie2/test.yml
@@ -47,17 +47,17 @@
     - bowtie2_align
   files:
     - path: output/bowtie2/bowtie2/NC_010473.1.bt2
-      md5sum: 90f0b7aa5bbaeaaa999839ac13ad203c
+      md5sum: 4db22d92e72111a5fbf609b3d9a43015
     - path: output/bowtie2/bowtie2/NC_010473.2.bt2
-      md5sum: bfd10c5319c6a0dbc540fd789254a5dd
+      md5sum: f4429ec74ee0064732c4bb26718a3eb1
     - path: output/bowtie2/bowtie2/NC_010473.3.bt2
       md5sum: cd201e81724f3099131aec16ef2cc53b
     - path: output/bowtie2/bowtie2/NC_010473.4.bt2
       md5sum: bbb9d6d21ad765d135f95290204e8433
     - path: output/bowtie2/bowtie2/NC_010473.rev.1.bt2
-      md5sum: 44f719c2fe42e1f35d54e798775846d1
+      md5sum: 4ccfee8857c3b1c69857e5ecdef597aa
     - path: output/bowtie2/bowtie2/NC_010473.rev.2.bt2
-      md5sum: f3c398bba5158f4039334a932d79c051
+      md5sum: 5e8fb4af677eb3a57a40e76dc3f6db72
     - path: output/bowtie2/test.bowtie2.log
       md5sum: 9f9eb40b5f57e0f2d5c874f2c1b5cfd5
     - path: output/bowtie2/test.bam