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add cutadapt module
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24
tools/cutadapt/main.nf
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24
tools/cutadapt/main.nf
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process cutadapt {
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tag "${sample_id}"
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container 'quay.io/biocontainers/cutadapt:1.16--py27_1'
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input:
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tuple sample_id, file(input_forward_fq), file(input_reverse_fq)
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output:
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tuple sample_id, file(output_forward_fq), file(output_reverse_fq)
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script:
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"""
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cutadapt \
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-j ${task.cpus} \
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-q $params.cutadapt_min_quality \
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--minimum-length $params.cutadapt_min_length \
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--pair-filter=any \
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--output ${forward_fq} \
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--paired-output ${reverse_fq} '$input_forward_fq' '$input_reverse_fq'
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cutadapt --version &> v_cutadapt.txt
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"""
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}
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40
tools/cutadapt/meta.yml
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tools/cutadapt/meta.yml
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name: Cutadapt
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description: cutadapt removes adapter sequences from high-throughput sequencing reads
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keywords:
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- Quality Control
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- QC
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- Adapters
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tools:
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- fastqc:
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description: |
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Cutadapt finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence
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from your high-throughput sequencing reads.
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Cleaning your data in this way is often required: Reads from small-RNA sequencing contain the 3’
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sequencing adapter because the read is longer than the molecule that is sequenced. Amplicon reads
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start with a primer sequence. Poly-A tails are useful for pulling out RNA from your sample, but
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often you don’t want them to be in your reads.
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homepage: https://cutadapt.readthedocs.io/en/stable/
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documentation: https://cutadapt.readthedocs.io/en/stable/
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input:
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- - sample_id:
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type: string
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description: Sample identifier
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- input_forward_fq:
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type: file
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description: Input FastQ forward read file of the pair
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- input_reverse_fq:
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type: file
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description: Input FastQ reverse read file of the pair
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output:
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- - sample_id:
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type: string
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description: Sample identifier
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- output_forward_fq:
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type: file
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description: Output FastQ forward read file of the pair
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- output_reverse_fq:
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type: file
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description: Output FastQ reverse read file of the pair
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authors:
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- @piotr-faba-ardigen
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