Merge branch 'master' into bat-test

This commit is contained in:
Gregor Sturm 2022-02-07 17:35:47 +01:00 committed by GitHub
commit 9c1278b4c3
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452 changed files with 2962 additions and 115 deletions

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@ -1,10 +1,14 @@
# List the start up tasks. Learn more https://www.gitpod.io/docs/config-start-tasks/ image: nfcore/gitpod:latest
tasks:
- name: Install Nextflow
init: |
curl -s https://get.nextflow.io | bash
sudo mv nextflow /usr/local/bin
- name: Install pytest-workflow vscode:
init: | extensions: # based on nf-core.nf-core-extensionpack
pip install pytest-workflow - codezombiech.gitignore # Language support for .gitignore files
# - cssho.vscode-svgviewer # SVG viewer
- davidanson.vscode-markdownlint # Markdown/CommonMark linting and style checking for Visual Studio Code
- eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed
- EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files
- Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar
- mechatroner.rainbow-csv # Highlight columns in csv files in different colors
# - nextflow.nextflow # Nextflow syntax highlighting
- oderwat.indent-rainbow # Highlight indentation level
- streetsidesoftware.code-spell-checker # Spelling checker for source code

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@ -1,3 +1,4 @@
repository_type: modules
bump-versions: bump-versions:
rseqc/junctionannotation: False rseqc/junctionannotation: False
rseqc/bamstat: False rseqc/bamstat: False

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@ -15,6 +15,9 @@ process ABACAS {
tuple val(meta), path('*.abacas*'), emit: results tuple val(meta), path('*.abacas*'), emit: results
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process ADAPTERREMOVAL {
tuple val(meta), path('*.log') , emit: log tuple val(meta), path('*.log') , emit: log
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process AGRVATE {
path "${fasta.baseName}-results" , emit: results_dir path "${fasta.baseName}-results" , emit: results_dir
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -16,6 +16,9 @@ process ALLELECOUNTER {
tuple val(meta), path("*.alleleCount"), emit: allelecount tuple val(meta), path("*.alleleCount"), emit: allelecount
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -18,6 +18,9 @@ process AMPS {
path "results/pdf_candidate_profiles/" , emit: candidate_pdfs path "results/pdf_candidate_profiles/" , emit: candidate_pdfs
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

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@ -17,6 +17,9 @@ process ARRIBA {
tuple val(meta), path("*.fusions.discarded.tsv"), emit: fusions_fail tuple val(meta), path("*.fusions.discarded.tsv"), emit: fusions_fail
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process ARTIC_GUPPYPLEX {
tuple val(meta), path("*.fastq.gz"), emit: fastq tuple val(meta), path("*.fastq.gz"), emit: fastq
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -31,6 +31,9 @@ process ARTIC_MINION {
tuple val(meta), path("*.json"), optional:true , emit: json tuple val(meta), path("*.json"), optional:true , emit: json
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}" prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process ASSEMBLYSCAN {
tuple val(meta), path("*.json"), emit: json tuple val(meta), path("*.json"), emit: json
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -19,6 +19,9 @@ process ATAQV_ATAQV {
tuple val(meta), path("*.problems") , emit: problems, optional: true tuple val(meta), path("*.problems") , emit: problems, optional: true
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -25,6 +25,9 @@ process BAKTA {
tuple val(meta), path("${prefix}.tsv") , emit: tsv tuple val(meta), path("${prefix}.tsv") , emit: tsv
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}" prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BAMALIGNCLEANER {
tuple val(meta), path("*.bam"), emit: bam tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

38
modules/bamcmp/main.nf Normal file
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@ -0,0 +1,38 @@
def VERSION = '2.2'
process BAMCMP {
label 'process_low'
conda (params.enable_conda ? "bioconda::bamcmp=2.2" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/bamcmp:2.2--h05f6578_0' :
'quay.io/biocontainers/bamcmp:2.2--h05f6578_0' }"
input:
tuple val(meta), path(sample), path(contaminant)
output:
tuple val(meta), path("*primary.bam") , emit: bam
tuple val(meta), path("*contamination.bam"), emit: contamination_bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
"""
bamcmp \\
-1 $sample \\
-2 $contaminant \\
-A ${prefix}_primary.bam \\
-B ${prefix}_contamination.bam \\
$args
cat <<-END_VERSIONS > versions.yml
"${task.process}":
bamcmp: $VERSION
END_VERSIONS
"""
}

57
modules/bamcmp/meta.yml Normal file
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@ -0,0 +1,57 @@
name: bamcmp
description: Bamcmp (Bam Compare) is a tool for assigning reads between a primary genome and a contamination genome. For instance, filtering out mouse reads from patient derived xenograft mouse models (PDX).
keywords:
- filter
- xenograft
- host
- graft
- contamination
- mouse
tools:
- bamcmp:
description: Bamcmp is a tool for deconvolving host and graft reads, using two bam files. Reads should be mapped to two genomes, and the mapped,
sorted bam files supplied to the tool. It is highly recommended to use the "-s as" option not the "-s mapq" option, else
reads which multimap to the contamination genome will be spuriously kept.
homepage: https://github.com/CRUKMI-ComputationalBiology/bamcmp
documentation: https://github.com/CRUKMI-ComputationalBiology/bamcmp
tool_dev_url: https://github.com/CRUKMI-ComputationalBiology/bamcmp
doi: "10.1158/1541-7786.MCR-16-0431"
licence: ['GPL v3']
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test']
- sample:
type: file
description: BAM/CRAM/SAM file with the reads aligned to the primary genome (the one you want to keep)
pattern: "*.{bam,cram,sam}"
- contaminant:
type: file
description: BAM/CRAM/SAM file with the reads aligned to the contaminant genome (the one you want to filter out)
pattern: "*.{bam,cram,sam}"
output:
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
- bam:
type: file
description: Bam file containing the reads which align better to the primary genome.
pattern: "*.{bam,cram,sam}"
- bam:
type: file
description: Bam file containing the reads which align better to the contaminant genome.
pattern: "*.{bam,cram,sam}"
authors:
- "@kmurat1"
- "@sppearce"

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@ -0,0 +1,41 @@
process BAMTOOLS_CONVERT {
tag "$meta.id"
label 'process_low'
conda (params.enable_conda ? "bioconda::bamtools=2.5.1" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/bamtools:2.5.1--h9a82719_9' :
'quay.io/biocontainers/bamtools:2.5.1--h9a82719_9' }"
input:
tuple val(meta), path(bam)
output:
tuple val(meta), path("*.{bed,fasta,fastq,json,pileup,sam,yaml}"), emit: data
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def test = args ==~ /-format (bed|fasta|fastq|json|pileup|sam|yaml)/
if ( test == false ) error "-format option must be provided in args. Possible values: bed fasta fastq json pileup sam yaml"
m = args =~ /-format ([a-z]+)/
ext = m[0][1]
"""
bamtools \\
convert \\
$args \\
-in $bam \\
-out ${prefix}.${ext}
cat <<-END_VERSIONS > versions.yml
"${task.process}":
bamtools: \$( bamtools --version | grep -e 'bamtools' | sed 's/^.*bamtools //' )
END_VERSIONS
"""
}

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@ -0,0 +1,52 @@
name: bamtools_convert
description: BamTools provides both a programmer's API and an end-user's toolkit for handling BAM files.
keywords:
- bamtools
- bamtools/convert
- bam
- convert
- bed
- fasta
- fastq
- json
- pileup
- sam
- yaml
tools:
- bamtools:
description: C++ API & command-line toolkit for working with BAM data
homepage: http://github.com/pezmaster31/bamtools
documentation: https://github.com/pezmaster31/bamtools/wiki
tool_dev_url: http://github.com/pezmaster31/bamtools
doi: ""
licence: ['MIT']
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- bam:
type: file
description: BAM file
pattern: "*.bam"
output:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
## TODO nf-core: Delete / customise this example output
- out:
type: file
description: The data in the asked format (bed, fasta, fastq, json, pileup, sam, yaml)
pattern: "*.{bed,fasta,fastq,json,pileup,sam,yaml}"
authors:
- "@sguizard"

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@ -14,6 +14,9 @@ process BAMTOOLS_SPLIT {
tuple val(meta), path("*.bam"), emit: bam tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BAMUTIL_TRIMBAM {
tuple val(meta), path("*.bam"), emit: bam tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process BANDAGE_IMAGE {
tuple val(meta), path('*.svg'), emit: svg tuple val(meta), path('*.svg'), emit: svg
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -16,6 +16,9 @@ process BBMAP_ALIGN {
tuple val(meta), path("*.log"), emit: log tuple val(meta), path("*.log"), emit: log
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -16,6 +16,9 @@ process BBMAP_BBDUK {
tuple val(meta), path('*.log') , emit: log tuple val(meta), path('*.log') , emit: log
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -20,6 +20,9 @@ process BBMAP_BBSPLIT {
tuple val(meta), path('*txt') , optional:true, emit: stats tuple val(meta), path('*txt') , optional:true, emit: stats
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BBMAP_INDEX {
path 'ref' , emit: index path 'ref' , emit: index
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

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@ -14,6 +14,9 @@ process BCFTOOLS_CONCAT {
tuple val(meta), path("*.gz"), emit: vcf tuple val(meta), path("*.gz"), emit: vcf
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}" prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_CONSENSUS {
tuple val(meta), path('*.fa'), emit: fasta tuple val(meta), path('*.fa'), emit: fasta
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_FILTER {
tuple val(meta), path("*.gz"), emit: vcf tuple val(meta), path("*.gz"), emit: vcf
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process BCFTOOLS_INDEX {
tuple val(meta), path("*.tbi"), optional:true, emit: tbi tuple val(meta), path("*.tbi"), optional:true, emit: tbi
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_ISEC {
tuple val(meta), path("${prefix}"), emit: results tuple val(meta), path("${prefix}"), emit: results
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}" prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_MERGE {
tuple val(meta), path("*.gz"), emit: vcf tuple val(meta), path("*.gz"), emit: vcf
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}" prefix = task.ext.prefix ?: "${meta.id}"

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@ -19,6 +19,9 @@ process BCFTOOLS_MPILEUP {
tuple val(meta), path("*.mpileup") , emit: mpileup, optional: true tuple val(meta), path("*.mpileup") , emit: mpileup, optional: true
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: '' def args2 = task.ext.args2 ?: ''

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@ -15,6 +15,9 @@ process BCFTOOLS_NORM {
tuple val(meta), path("*.gz") , emit: vcf tuple val(meta), path("*.gz") , emit: vcf
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -8,30 +8,32 @@ process BCFTOOLS_QUERY {
'quay.io/biocontainers/bcftools:1.14--h88f3f91_0' }" 'quay.io/biocontainers/bcftools:1.14--h88f3f91_0' }"
input: input:
tuple val(meta), path(vcf), path(index) tuple val(meta), path(vcf), path(tbi)
path(regions) path regions
path(targets) path targets
path(samples) path samples
output: output:
tuple val(meta), path("*.gz") , emit: vcf tuple val(meta), path("*.txt"), emit: txt
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"
def regions_file = regions ? "--regions-file ${regions}" : "" def regions_file = regions ? "--regions-file ${regions}" : ""
def targets_file = targets ? "--targets-file ${targets}" : "" def targets_file = targets ? "--targets-file ${targets}" : ""
def samples_file = samples ? "--samples-file ${samples}" : "" def samples_file = samples ? "--samples-file ${samples}" : ""
""" """
bcftools query \\ bcftools query \\
--output ${prefix}.vcf.gz \\ --output ${prefix}.txt \\
${regions_file} \\ $regions_file \\
${targets_file} \\ $targets_file \\
${samples_file} \\ $samples_file \\
$args \\ $args \\
${vcf} $vcf
cat <<-END_VERSIONS > versions.yml cat <<-END_VERSIONS > versions.yml
"${task.process}": "${task.process}":

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@ -23,22 +23,20 @@ input:
type: file type: file
description: | description: |
The vcf file to be qeuried. The vcf file to be qeuried.
e.g. 'file.vcf' pattern: "*.{vcf.gz, vcf}"
- index: - tbi:
type: file type: file
description: | description: |
The tab index for the VCF file to be inspected. The tab index for the VCF file to be inspected.
e.g. 'file.tbi' pattern: "*.tbi"
- regions: - regions:
type: file type: file
description: | description: |
Optionally, restrict the operation to regions listed in this file. Optionally, restrict the operation to regions listed in this file.
e.g. 'file.vcf'
- targets: - targets:
type: file type: file
description: | description: |
Optionally, restrict the operation to regions listed in this file (doesn't rely upon index files) Optionally, restrict the operation to regions listed in this file (doesn't rely upon index files)
e.g. 'file.vcf'
- samples: - samples:
type: file type: file
description: | description: |
@ -50,13 +48,14 @@ output:
description: | description: |
Groovy Map containing sample information Groovy Map containing sample information
e.g. [ id:'test', single_end:false ] e.g. [ id:'test', single_end:false ]
- vcf: - txt:
type: file type: file
description: VCF query output file description: BCFTools query output file
pattern: "*.{vcf.gz}" pattern: "*.txt"
- versions: - versions:
type: file type: file
description: File containing software versions description: File containing software versions
pattern: "versions.yml" pattern: "versions.yml"
authors: authors:
- "@abhi18av" - "@abhi18av"
- "@drpatelh"

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@ -16,6 +16,9 @@ process BCFTOOLS_REHEADER {
tuple val(meta), path("*.vcf.gz"), emit: vcf tuple val(meta), path("*.vcf.gz"), emit: vcf
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_SORT {
tuple val(meta), path("*.gz"), emit: vcf tuple val(meta), path("*.gz"), emit: vcf
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_STATS {
tuple val(meta), path("*stats.txt"), emit: stats tuple val(meta), path("*stats.txt"), emit: stats
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -17,6 +17,9 @@ process BCFTOOLS_VIEW {
tuple val(meta), path("*.gz") , emit: vcf tuple val(meta), path("*.gz") , emit: vcf
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process BEDTOOLS_BAMTOBED {
tuple val(meta), path("*.bed"), emit: bed tuple val(meta), path("*.bed"), emit: bed
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -15,6 +15,9 @@ process BEDTOOLS_COMPLEMENT {
tuple val(meta), path('*.bed'), emit: bed tuple val(meta), path('*.bed'), emit: bed
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -16,6 +16,9 @@ process BEDTOOLS_GENOMECOV {
tuple val(meta), path("*.${extension}"), emit: genomecov tuple val(meta), path("*.${extension}"), emit: genomecov
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def args_list = args.tokenize() def args_list = args.tokenize()

View file

@ -15,6 +15,9 @@ process BEDTOOLS_GETFASTA {
path "*.fa" , emit: fasta path "*.fa" , emit: fasta
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${bed.baseName}" def prefix = task.ext.prefix ?: "${bed.baseName}"

View file

@ -15,6 +15,9 @@ process BEDTOOLS_INTERSECT {
tuple val(meta), path("*.${extension}"), emit: intersect tuple val(meta), path("*.${extension}"), emit: intersect
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -15,6 +15,9 @@ process BEDTOOLS_MAKEWINDOWS {
tuple val(meta), path("*.tab"), emit: tab tuple val(meta), path("*.tab"), emit: tab
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -15,6 +15,9 @@ process BEDTOOLS_MASKFASTA {
tuple val(meta), path("*.fa"), emit: fasta tuple val(meta), path("*.fa"), emit: fasta
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process BEDTOOLS_MERGE {
tuple val(meta), path('*.bed'), emit: bed tuple val(meta), path('*.bed'), emit: bed
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -15,6 +15,9 @@ process BEDTOOLS_SLOP {
tuple val(meta), path("*.bed"), emit: bed tuple val(meta), path("*.bed"), emit: bed
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -15,6 +15,9 @@ process BEDTOOLS_SORT {
tuple val(meta), path("*.${extension}"), emit: sorted tuple val(meta), path("*.${extension}"), emit: sorted
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process BEDTOOLS_SUBTRACT {
tuple val(meta), path("*.bed"), emit: bed tuple val(meta), path("*.bed"), emit: bed
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -17,6 +17,9 @@ process BISMARK_ALIGN {
tuple val(meta), path("*fq.gz") , optional:true, emit: unmapped tuple val(meta), path("*fq.gz") , optional:true, emit: unmapped
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -15,6 +15,9 @@ process BISMARK_DEDUPLICATE {
tuple val(meta), path("*.deduplication_report.txt"), emit: report tuple val(meta), path("*.deduplication_report.txt"), emit: report
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process BISMARK_GENOMEPREPARATION {
path "BismarkIndex" , emit: index path "BismarkIndex" , emit: index
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -19,6 +19,9 @@ process BISMARK_METHYLATIONEXTRACTOR {
tuple val(meta), path("*.M-bias.txt") , emit: mbias tuple val(meta), path("*.M-bias.txt") , emit: mbias
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def seqtype = meta.single_end ? '-s' : '-p' def seqtype = meta.single_end ? '-s' : '-p'

View file

@ -14,6 +14,9 @@ process BISMARK_REPORT {
tuple val(meta), path("*report.{html,txt}"), emit: report tuple val(meta), path("*report.{html,txt}"), emit: report
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -17,6 +17,9 @@ process BISMARK_SUMMARY {
path "*report.{html,txt}", emit: summary path "*report.{html,txt}", emit: summary
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -15,6 +15,9 @@ process BLAST_BLASTN {
tuple val(meta), path('*.blastn.txt'), emit: txt tuple val(meta), path('*.blastn.txt'), emit: txt
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process BLAST_MAKEBLASTDB {
path 'blast_db' , emit: db path 'blast_db' , emit: db
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -17,6 +17,9 @@ process BOWTIE_ALIGN {
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
tuple val(meta), path('*fastq.gz'), optional:true, emit: fastq tuple val(meta), path('*fastq.gz'), optional:true, emit: fastq
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: '' def args2 = task.ext.args2 ?: ''

View file

@ -14,6 +14,9 @@ process BOWTIE_BUILD {
path 'bowtie' , emit: index path 'bowtie' , emit: index
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -18,6 +18,9 @@ process BOWTIE2_ALIGN {
tuple val(meta), path('*fastq.gz'), emit: fastq, optional:true tuple val(meta), path('*fastq.gz'), emit: fastq, optional:true
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: '' def args2 = task.ext.args2 ?: ''

View file

@ -14,6 +14,9 @@ process BOWTIE2_BUILD {
path 'bowtie2' , emit: index path 'bowtie2' , emit: index
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -15,6 +15,9 @@ process BWA_ALN {
tuple val(meta), path("*.sai"), emit: sai tuple val(meta), path("*.sai"), emit: sai
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process BWA_INDEX {
path "bwa" , emit: index path "bwa" , emit: index
path "versions.yml", emit: versions path "versions.yml", emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -16,6 +16,9 @@ process BWA_MEM {
tuple val(meta), path("*.bam"), emit: bam tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: '' def args2 = task.ext.args2 ?: ''

View file

@ -15,6 +15,9 @@ process BWA_SAMPE {
tuple val(meta), path("*.bam"), emit: bam tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -15,6 +15,9 @@ process BWA_SAMSE {
tuple val(meta), path("*.bam"), emit: bam tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process BWAMEM2_INDEX {
path "bwamem2" , emit: index path "bwamem2" , emit: index
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -16,6 +16,9 @@ process BWAMEM2_MEM {
tuple val(meta), path("*.bam"), emit: bam tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: '' def args2 = task.ext.args2 ?: ''

View file

@ -15,6 +15,9 @@ process BWAMETH_ALIGN {
tuple val(meta), path("*.bam"), emit: bam tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: '' def args2 = task.ext.args2 ?: ''

View file

@ -14,6 +14,9 @@ process BWAMETH_INDEX {
path "bwameth" , emit: index path "bwameth" , emit: index
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -14,6 +14,9 @@ process CAT_CAT {
path "${file_out}*" , emit: file_out path "${file_out}*" , emit: file_out
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: '' def args2 = task.ext.args2 ?: ''

View file

@ -14,6 +14,9 @@ process CAT_FASTQ {
tuple val(meta), path("*.merged.fastq.gz"), emit: reads tuple val(meta), path("*.merged.fastq.gz"), emit: reads
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -1 +1,2 @@
cellranger-*.tar.gz cellranger-*.tar.gz
bcl2fastq2-*.zip

View file

@ -1,15 +1,22 @@
# Dockerfile to create container with Cell Ranger v6.1.2
# Push to nfcore/cellranger:<VER>
FROM continuumio/miniconda3:4.8.2 FROM continuumio/miniconda3:4.8.2
LABEL authors="Gisela Gabernet <gisela.gabernet@gmail.com>" \ LABEL authors="Gisela Gabernet <gisela.gabernet@gmail.com>" \
description="Docker image containing Cell Ranger" description="Docker image containing Cell Ranger"
# Disclaimer: this container is not provided nor supported by 10x Genomics. # Disclaimer: this container is not provided nor supported by Illumina or 10x Genomics.
# Install procps and clean apt cache # Install procps and clean apt cache
RUN apt-get update --allow-releaseinfo-change \ RUN apt-get update --allow-releaseinfo-change \
&& apt-get install -y procps \ && apt-get install -y \
cpio \
procps \
rpm2cpio \
unzip \
&& apt-get clean -y && rm -rf /var/lib/apt/lists/* && apt-get clean -y && rm -rf /var/lib/apt/lists/*
# Copy pre-downloaded cellranger file # Copy pre-downloaded cellranger file
ENV CELLRANGER_VER 6.0.2 ENV CELLRANGER_VER=6.1.2
COPY cellranger-$CELLRANGER_VER.tar.gz /opt/cellranger-$CELLRANGER_VER.tar.gz COPY cellranger-$CELLRANGER_VER.tar.gz /opt/cellranger-$CELLRANGER_VER.tar.gz
# Install cellranger # Install cellranger

View file

@ -1,18 +1,24 @@
# Updating the docker container and making a new module release # Updating the docker container and making a new module release
Cell Ranger is a commercial tool by 10X Genomics. The container provided for the cellranger nf-core module is not provided nor supported by 10x Genomics. Updating the Cell Ranger version in the container and pushing the update to Dockerhub needs to be done manually. Cell Ranger is a commercial tool from 10X Genomics. The container provided for the cellranger nf-core module is not provided nor supported by 10x Genomics. Updating the Cell Ranger versions in the container and pushing the update to Dockerhub needs to be done manually.
1. Navigate to the [Cell Ranger download page](https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest) and download the tar ball of the desired Cell Ranger version with `curl` or `wget`. Place this file in the same folder where the Dockerfile lies. 1. Navigate to the appropriate download page.
- [Cell Ranger](https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest): download the tar ball of the desired Cell Ranger version with `curl` or `wget`. Place this file in the same folder where the Dockerfile lies.
2. Edit the Dockerfile: update the Cell Ranger version in this line: 2. Edit the Dockerfile. Update the Cell Ranger versions in this line:
```bash ```bash
ENV CELLRANGER_VER <VERSION> ENV CELLRANGER_VER=<VERSION>
``` ```
3. Create the container: 3. Create and test the container:
```bash ```bash
docker build . -t nfcore/cellranger:<VERSION> docker build . -t nfcore/cellranger:<VERSION>
```
4. Access rights are needed to push the container to the Dockerhub nfcore organization, please ask a core team member to do so.
```bash
docker push nfcore/cellranger:<VERSION> docker push nfcore/cellranger:<VERSION>
``` ```

View file

@ -5,7 +5,7 @@ process CELLRANGER_COUNT {
if (params.enable_conda) { if (params.enable_conda) {
exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers." exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers."
} }
container "nfcore/cellranger:6.0.2" container "nfcore/cellranger:6.1.2"
input: input:
tuple val(meta), path(reads) tuple val(meta), path(reads)
@ -15,6 +15,9 @@ process CELLRANGER_COUNT {
path("sample-${meta.gem}/outs/*"), emit: outs path("sample-${meta.gem}/outs/*"), emit: outs
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def sample_arg = meta.samples.unique().join(",") def sample_arg = meta.samples.unique().join(",")

View file

@ -0,0 +1,40 @@
# Dockerfile to create container with Cell Ranger v6.1.2 and bcl2fastq v2.20.0
# Push to nfcore/cellrangermkfastq:<VER>
FROM continuumio/miniconda3:4.8.2
LABEL authors="Regina Reynolds, Gisela Gabernet <gisela.gabernet@gmail.com>" \
description="Docker image containing bcl2fastq2 and Cell Ranger"
# Disclaimer: this container is not provided nor supported by Illumina or 10x Genomics.
# Install procps and clean apt cache
RUN apt-get update --allow-releaseinfo-change \
&& apt-get install -y \
cpio \
procps \
rpm2cpio \
unzip \
&& apt-get clean -y && rm -rf /var/lib/apt/lists/*
# Copy pre-downloaded bcl2fastq2 and cellranger file
ENV BCL2FASTQ2_VER=v2-20-0-linux-x86-64 \
CELLRANGER_VER=6.1.2
COPY bcl2fastq2-$BCL2FASTQ2_VER.zip /tmp/bcl2fastq2-$BCL2FASTQ2_VER.zip
COPY cellranger-$CELLRANGER_VER.tar.gz /opt/cellranger-$CELLRANGER_VER.tar.gz
# Install bcl2fastq2
RUN \
cd /tmp && \
unzip bcl2fastq2-$BCL2FASTQ2_VER.zip && \
mv *.rpm bcl2fastq2-$BCL2FASTQ2_VER.rpm && \
rpm2cpio ./bcl2fastq2-$BCL2FASTQ2_VER.rpm | cpio -idmv && \
export PATH=/tmp/usr/local/bin/:$PATH && \
ln -s /tmp/usr/local/bin/bcl2fastq /usr/bin/bcl2fastq && \
rm -rf bcl2fastq2-$BCL2FASTQ2_VER.*
# Install cellranger
RUN \
cd /opt && \
tar -xzvf cellranger-$CELLRANGER_VER.tar.gz && \
export PATH=/opt/cellranger-$CELLRANGER_VER:$PATH && \
ln -s /opt/cellranger-$CELLRANGER_VER/cellranger /usr/bin/cellranger && \
rm -rf /opt/cellranger-$CELLRANGER_VER.tar.gz

View file

@ -0,0 +1,26 @@
# Updating the docker container and making a new module release
Bcl2fastq2 and Cell Ranger are commercial tools from Illumina and 10X Genomics, respectively. The container provided for the cellranger nf-core module is not provided nor supported by either Illumina or 10x Genomics. Updating the bcl2fastq2 or Cell Ranger versions in the container and pushing the update to Dockerhub needs to be done manually.
1. Navigate to the appropriate download pages.
- [bcl2fastq2](https://emea.support.illumina.com/sequencing/sequencing_software/bcl2fastq-conversion-software.html): download the linux rpm installer of the desired bcl2fastq2 version with `curl` or `wget`. Place this file in the same folder where the Dockerfile lies.
- [Cell Ranger](https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest): download the tar ball of the desired Cell Ranger version with `curl` or `wget`. Place this file in the same folder where the Dockerfile lies.
2. Edit the Dockerfile. Update the bcl2fastq2 and Cell Ranger versions in this line:
```bash
ENV BCL2FASTQ2_VER=<VERSION> \
CELLRANGER_VER=<VERSION>
```
3. Create and test the container:
```bash
docker build . -t nfcore/cellrangermkfastq:<CELLRANGER_VERSION>
```
4. Access rights are needed to push the container to the Dockerhub nfcore organization, please ask a core team member to do so.
```bash
docker push nfcore/cellrangermkfastq:<CELLRANGER_VERSION>
```

View file

@ -5,7 +5,7 @@ process CELLRANGER_MKFASTQ {
if (params.enable_conda) { if (params.enable_conda) {
exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers." exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers."
} }
container "litd/docker-cellranger:v6.1.1" // FIXME Add bcl2fastq to nf-core docker image container "nfcore/cellrangermkfastq:6.1.2"
input: input:
path bcl path bcl
@ -13,14 +13,17 @@ process CELLRANGER_MKFASTQ {
output: output:
path "versions.yml", emit: versions path "versions.yml", emit: versions
path "*.fastq.gz" , emit: fastq path "${bcl.getSimpleName()}/outs/fastq_path/*.fastq.gz" , emit: fastq
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """
cellranger mkfastq --id=${bcl.getSimpleName()} \ cellranger mkfastq --id=${bcl.getSimpleName()} \
--run=$bcl \ --run=$bcl \
--csv=$csv --csv=$csv \
$args $args
cat <<-END_VERSIONS > versions.yml cat <<-END_VERSIONS > versions.yml
@ -28,4 +31,15 @@ process CELLRANGER_MKFASTQ {
cellranger: \$(echo \$( cellranger --version 2>&1) | sed 's/^.*[^0-9]\\([0-9]*\\.[0-9]*\\.[0-9]*\\).*\$/\\1/' ) cellranger: \$(echo \$( cellranger --version 2>&1) | sed 's/^.*[^0-9]\\([0-9]*\\.[0-9]*\\.[0-9]*\\).*\$/\\1/' )
END_VERSIONS END_VERSIONS
""" """
stub:
"""
mkdir -p "${bcl.getSimpleName()}/outs/fastq_path/"
touch ${bcl.getSimpleName()}/outs/fastq_path/fake_file.fastq.gz
cat <<-END_VERSIONS > versions.yml
"${task.process}":
cellranger: \$(echo \$( cellranger --version 2>&1) | sed 's/^.*[^0-9]\\([0-9]*\\.[0-9]*\\.[0-9]*\\).*\$/\\1/' )
END_VERSIONS
"""
} }

View file

@ -5,7 +5,7 @@ process CELLRANGER_MKGTF {
if (params.enable_conda) { if (params.enable_conda) {
exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers." exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers."
} }
container "nfcore/cellranger:6.0.2" container "nfcore/cellranger:6.1.2"
input: input:
path gtf path gtf
@ -14,6 +14,9 @@ process CELLRANGER_MKGTF {
path "*.filtered.gtf", emit: gtf path "*.filtered.gtf", emit: gtf
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -5,7 +5,7 @@ process CELLRANGER_MKREF {
if (params.enable_conda) { if (params.enable_conda) {
exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers." exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers."
} }
container "nfcore/cellranger:6.0.2" container "nfcore/cellranger:6.1.2"
input: input:
path fasta path fasta
@ -16,6 +16,9 @@ process CELLRANGER_MKREF {
path "${reference_name}", emit: reference path "${reference_name}", emit: reference
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -16,6 +16,9 @@ process CHECKM_LINEAGEWF {
tuple val(meta), path("${prefix}.tsv"), emit: checkm_tsv tuple val(meta), path("${prefix}.tsv"), emit: checkm_tsv
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}" prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -23,6 +23,9 @@ process CHROMAP_CHROMAP {
tuple val(meta), path("*.pairs.gz") , optional:true, emit: pairs tuple val(meta), path("*.pairs.gz") , optional:true, emit: pairs
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: '' def args2 = task.ext.args2 ?: ''

View file

@ -15,6 +15,9 @@ process CHROMAP_INDEX {
path "*.index" , emit: index path "*.index" , emit: index
path "versions.yml", emit: versions path "versions.yml", emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = fasta.baseName def prefix = fasta.baseName

View file

@ -19,6 +19,9 @@ process CLONALFRAMEML {
tuple val(meta), path("*.position_cross_reference.txt"), emit: pos_ref tuple val(meta), path("*.position_cross_reference.txt"), emit: pos_ref
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -16,6 +16,9 @@ process CMSEQ_POLYMUT {
tuple val(meta), path("*.txt"), emit: polymut tuple val(meta), path("*.txt"), emit: polymut
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -20,6 +20,9 @@ process CNVKIT_BATCH {
tuple val(meta), path("*.cns"), emit: cns, optional: true tuple val(meta), path("*.cns"), emit: cns, optional: true
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def normal_args = normal ? "--normal $normal" : "" def normal_args = normal ? "--normal $normal" : ""

View file

@ -16,6 +16,9 @@ process COOLER_CLOAD {
tuple val(meta), val(cool_bin), path("*.cool"), emit: cool tuple val(meta), val(cool_bin), path("*.cool"), emit: cool
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -16,6 +16,9 @@ process COOLER_DIGEST {
path "*.bed" , emit: bed path "*.bed" , emit: bed
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -15,6 +15,9 @@ process COOLER_DUMP {
tuple val(meta), path("*.bedpe"), emit: bedpe tuple val(meta), path("*.bedpe"), emit: bedpe
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process COOLER_MERGE {
tuple val(meta), path("*.cool"), emit: cool tuple val(meta), path("*.cool"), emit: cool
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process COOLER_ZOOMIFY {
tuple val(meta), path("*.mcool"), emit: mcool tuple val(meta), path("*.mcool"), emit: mcool
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -16,6 +16,9 @@ process CSVTK_CONCAT {
tuple val(meta), path("${prefix}.${out_extension}"), emit: csv tuple val(meta), path("${prefix}.${out_extension}"), emit: csv
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}" prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -16,6 +16,9 @@ process CSVTK_SPLIT {
tuple val(meta), path("*.${out_extension}"), emit: split_csv tuple val(meta), path("*.${out_extension}"), emit: split_csv
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -15,6 +15,9 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
path "software_versions_mqc.yml", emit: mqc_yml path "software_versions_mqc.yml", emit: mqc_yml
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
template 'dumpsoftwareversions.py' template 'dumpsoftwareversions.py'

View file

@ -15,6 +15,9 @@ process CUSTOM_GETCHROMSIZES {
path '*.fai' , emit: fai path '*.fai' , emit: fai
path "versions.yml", emit: versions path "versions.yml", emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
""" """

View file

@ -15,6 +15,9 @@ process CUTADAPT {
tuple val(meta), path('*.log') , emit: log tuple val(meta), path('*.log') , emit: log
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -17,6 +17,9 @@ process DAMAGEPROFILER {
tuple val(meta), path("${prefix}"), emit: results tuple val(meta), path("${prefix}"), emit: results
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}" prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -26,6 +26,9 @@ process DASTOOL_DASTOOL {
tuple val(meta), path("*.seqlength") , optional: true, emit: seqlength tuple val(meta), path("*.seqlength") , optional: true, emit: seqlength
path "versions.yml" , emit: versions path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script: script:
def args = task.ext.args ?: '' def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}" def prefix = task.ext.prefix ?: "${meta.id}"

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