Merge branch 'master' into bat-test

This commit is contained in:
Gregor Sturm 2022-02-07 17:35:47 +01:00 committed by GitHub
commit 9c1278b4c3
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GPG key ID: 4AEE18F83AFDEB23
452 changed files with 2962 additions and 115 deletions

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@ -1,10 +1,14 @@
# List the start up tasks. Learn more https://www.gitpod.io/docs/config-start-tasks/
tasks:
- name: Install Nextflow
init: |
curl -s https://get.nextflow.io | bash
sudo mv nextflow /usr/local/bin
image: nfcore/gitpod:latest
- name: Install pytest-workflow
init: |
pip install pytest-workflow
vscode:
extensions: # based on nf-core.nf-core-extensionpack
- codezombiech.gitignore # Language support for .gitignore files
# - cssho.vscode-svgviewer # SVG viewer
- davidanson.vscode-markdownlint # Markdown/CommonMark linting and style checking for Visual Studio Code
- eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed
- EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files
- Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar
- mechatroner.rainbow-csv # Highlight columns in csv files in different colors
# - nextflow.nextflow # Nextflow syntax highlighting
- oderwat.indent-rainbow # Highlight indentation level
- streetsidesoftware.code-spell-checker # Spelling checker for source code

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@ -1,3 +1,4 @@
repository_type: modules
bump-versions:
rseqc/junctionannotation: False
rseqc/bamstat: False

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@ -15,6 +15,9 @@ process ABACAS {
tuple val(meta), path('*.abacas*'), emit: results
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process ADAPTERREMOVAL {
tuple val(meta), path('*.log') , emit: log
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process AGRVATE {
path "${fasta.baseName}-results" , emit: results_dir
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -16,6 +16,9 @@ process ALLELECOUNTER {
tuple val(meta), path("*.alleleCount"), emit: allelecount
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -18,6 +18,9 @@ process AMPS {
path "results/pdf_candidate_profiles/" , emit: candidate_pdfs
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

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@ -17,6 +17,9 @@ process ARRIBA {
tuple val(meta), path("*.fusions.discarded.tsv"), emit: fusions_fail
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process ARTIC_GUPPYPLEX {
tuple val(meta), path("*.fastq.gz"), emit: fastq
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -31,6 +31,9 @@ process ARTIC_MINION {
tuple val(meta), path("*.json"), optional:true , emit: json
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process ASSEMBLYSCAN {
tuple val(meta), path("*.json"), emit: json
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -19,6 +19,9 @@ process ATAQV_ATAQV {
tuple val(meta), path("*.problems") , emit: problems, optional: true
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -25,6 +25,9 @@ process BAKTA {
tuple val(meta), path("${prefix}.tsv") , emit: tsv
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BAMALIGNCLEANER {
tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

38
modules/bamcmp/main.nf Normal file
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@ -0,0 +1,38 @@
def VERSION = '2.2'
process BAMCMP {
label 'process_low'
conda (params.enable_conda ? "bioconda::bamcmp=2.2" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/bamcmp:2.2--h05f6578_0' :
'quay.io/biocontainers/bamcmp:2.2--h05f6578_0' }"
input:
tuple val(meta), path(sample), path(contaminant)
output:
tuple val(meta), path("*primary.bam") , emit: bam
tuple val(meta), path("*contamination.bam"), emit: contamination_bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
"""
bamcmp \\
-1 $sample \\
-2 $contaminant \\
-A ${prefix}_primary.bam \\
-B ${prefix}_contamination.bam \\
$args
cat <<-END_VERSIONS > versions.yml
"${task.process}":
bamcmp: $VERSION
END_VERSIONS
"""
}

57
modules/bamcmp/meta.yml Normal file
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@ -0,0 +1,57 @@
name: bamcmp
description: Bamcmp (Bam Compare) is a tool for assigning reads between a primary genome and a contamination genome. For instance, filtering out mouse reads from patient derived xenograft mouse models (PDX).
keywords:
- filter
- xenograft
- host
- graft
- contamination
- mouse
tools:
- bamcmp:
description: Bamcmp is a tool for deconvolving host and graft reads, using two bam files. Reads should be mapped to two genomes, and the mapped,
sorted bam files supplied to the tool. It is highly recommended to use the "-s as" option not the "-s mapq" option, else
reads which multimap to the contamination genome will be spuriously kept.
homepage: https://github.com/CRUKMI-ComputationalBiology/bamcmp
documentation: https://github.com/CRUKMI-ComputationalBiology/bamcmp
tool_dev_url: https://github.com/CRUKMI-ComputationalBiology/bamcmp
doi: "10.1158/1541-7786.MCR-16-0431"
licence: ['GPL v3']
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test']
- sample:
type: file
description: BAM/CRAM/SAM file with the reads aligned to the primary genome (the one you want to keep)
pattern: "*.{bam,cram,sam}"
- contaminant:
type: file
description: BAM/CRAM/SAM file with the reads aligned to the contaminant genome (the one you want to filter out)
pattern: "*.{bam,cram,sam}"
output:
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
- bam:
type: file
description: Bam file containing the reads which align better to the primary genome.
pattern: "*.{bam,cram,sam}"
- bam:
type: file
description: Bam file containing the reads which align better to the contaminant genome.
pattern: "*.{bam,cram,sam}"
authors:
- "@kmurat1"
- "@sppearce"

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@ -0,0 +1,41 @@
process BAMTOOLS_CONVERT {
tag "$meta.id"
label 'process_low'
conda (params.enable_conda ? "bioconda::bamtools=2.5.1" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/bamtools:2.5.1--h9a82719_9' :
'quay.io/biocontainers/bamtools:2.5.1--h9a82719_9' }"
input:
tuple val(meta), path(bam)
output:
tuple val(meta), path("*.{bed,fasta,fastq,json,pileup,sam,yaml}"), emit: data
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def test = args ==~ /-format (bed|fasta|fastq|json|pileup|sam|yaml)/
if ( test == false ) error "-format option must be provided in args. Possible values: bed fasta fastq json pileup sam yaml"
m = args =~ /-format ([a-z]+)/
ext = m[0][1]
"""
bamtools \\
convert \\
$args \\
-in $bam \\
-out ${prefix}.${ext}
cat <<-END_VERSIONS > versions.yml
"${task.process}":
bamtools: \$( bamtools --version | grep -e 'bamtools' | sed 's/^.*bamtools //' )
END_VERSIONS
"""
}

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@ -0,0 +1,52 @@
name: bamtools_convert
description: BamTools provides both a programmer's API and an end-user's toolkit for handling BAM files.
keywords:
- bamtools
- bamtools/convert
- bam
- convert
- bed
- fasta
- fastq
- json
- pileup
- sam
- yaml
tools:
- bamtools:
description: C++ API & command-line toolkit for working with BAM data
homepage: http://github.com/pezmaster31/bamtools
documentation: https://github.com/pezmaster31/bamtools/wiki
tool_dev_url: http://github.com/pezmaster31/bamtools
doi: ""
licence: ['MIT']
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- bam:
type: file
description: BAM file
pattern: "*.bam"
output:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
## TODO nf-core: Delete / customise this example output
- out:
type: file
description: The data in the asked format (bed, fasta, fastq, json, pileup, sam, yaml)
pattern: "*.{bed,fasta,fastq,json,pileup,sam,yaml}"
authors:
- "@sguizard"

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@ -14,6 +14,9 @@ process BAMTOOLS_SPLIT {
tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BAMUTIL_TRIMBAM {
tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process BANDAGE_IMAGE {
tuple val(meta), path('*.svg'), emit: svg
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -16,6 +16,9 @@ process BBMAP_ALIGN {
tuple val(meta), path("*.log"), emit: log
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -16,6 +16,9 @@ process BBMAP_BBDUK {
tuple val(meta), path('*.log') , emit: log
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -20,6 +20,9 @@ process BBMAP_BBSPLIT {
tuple val(meta), path('*txt') , optional:true, emit: stats
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BBMAP_INDEX {
path 'ref' , emit: index
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

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@ -14,6 +14,9 @@ process BCFTOOLS_CONCAT {
tuple val(meta), path("*.gz"), emit: vcf
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_CONSENSUS {
tuple val(meta), path('*.fa'), emit: fasta
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_FILTER {
tuple val(meta), path("*.gz"), emit: vcf
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process BCFTOOLS_INDEX {
tuple val(meta), path("*.tbi"), optional:true, emit: tbi
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_ISEC {
tuple val(meta), path("${prefix}"), emit: results
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_MERGE {
tuple val(meta), path("*.gz"), emit: vcf
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}"

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@ -19,6 +19,9 @@ process BCFTOOLS_MPILEUP {
tuple val(meta), path("*.mpileup") , emit: mpileup, optional: true
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: ''

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@ -15,6 +15,9 @@ process BCFTOOLS_NORM {
tuple val(meta), path("*.gz") , emit: vcf
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -8,30 +8,32 @@ process BCFTOOLS_QUERY {
'quay.io/biocontainers/bcftools:1.14--h88f3f91_0' }"
input:
tuple val(meta), path(vcf), path(index)
path(regions)
path(targets)
path(samples)
tuple val(meta), path(vcf), path(tbi)
path regions
path targets
path samples
output:
tuple val(meta), path("*.gz") , emit: vcf
tuple val(meta), path("*.txt"), emit: txt
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def regions_file = regions ? "--regions-file ${regions}" : ""
def targets_file = targets ? "--targets-file ${targets}" : ""
def samples_file = samples ? "--samples-file ${samples}" : ""
"""
bcftools query \\
--output ${prefix}.vcf.gz \\
${regions_file} \\
${targets_file} \\
${samples_file} \\
--output ${prefix}.txt \\
$regions_file \\
$targets_file \\
$samples_file \\
$args \\
${vcf}
$vcf
cat <<-END_VERSIONS > versions.yml
"${task.process}":

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@ -23,22 +23,20 @@ input:
type: file
description: |
The vcf file to be qeuried.
e.g. 'file.vcf'
- index:
pattern: "*.{vcf.gz, vcf}"
- tbi:
type: file
description: |
The tab index for the VCF file to be inspected.
e.g. 'file.tbi'
pattern: "*.tbi"
- regions:
type: file
description: |
Optionally, restrict the operation to regions listed in this file.
e.g. 'file.vcf'
- targets:
type: file
description: |
Optionally, restrict the operation to regions listed in this file (doesn't rely upon index files)
e.g. 'file.vcf'
- samples:
type: file
description: |
@ -50,13 +48,14 @@ output:
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- vcf:
- txt:
type: file
description: VCF query output file
pattern: "*.{vcf.gz}"
description: BCFTools query output file
pattern: "*.txt"
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
authors:
- "@abhi18av"
- "@drpatelh"

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@ -16,6 +16,9 @@ process BCFTOOLS_REHEADER {
tuple val(meta), path("*.vcf.gz"), emit: vcf
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_SORT {
tuple val(meta), path("*.gz"), emit: vcf
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BCFTOOLS_STATS {
tuple val(meta), path("*stats.txt"), emit: stats
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -17,6 +17,9 @@ process BCFTOOLS_VIEW {
tuple val(meta), path("*.gz") , emit: vcf
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BEDTOOLS_BAMTOBED {
tuple val(meta), path("*.bed"), emit: bed
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process BEDTOOLS_COMPLEMENT {
tuple val(meta), path('*.bed'), emit: bed
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -16,6 +16,9 @@ process BEDTOOLS_GENOMECOV {
tuple val(meta), path("*.${extension}"), emit: genomecov
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def args_list = args.tokenize()

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@ -15,6 +15,9 @@ process BEDTOOLS_GETFASTA {
path "*.fa" , emit: fasta
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${bed.baseName}"

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@ -15,6 +15,9 @@ process BEDTOOLS_INTERSECT {
tuple val(meta), path("*.${extension}"), emit: intersect
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process BEDTOOLS_MAKEWINDOWS {
tuple val(meta), path("*.tab"), emit: tab
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process BEDTOOLS_MASKFASTA {
tuple val(meta), path("*.fa"), emit: fasta
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BEDTOOLS_MERGE {
tuple val(meta), path('*.bed'), emit: bed
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process BEDTOOLS_SLOP {
tuple val(meta), path("*.bed"), emit: bed
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process BEDTOOLS_SORT {
tuple val(meta), path("*.${extension}"), emit: sorted
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BEDTOOLS_SUBTRACT {
tuple val(meta), path("*.bed"), emit: bed
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -17,6 +17,9 @@ process BISMARK_ALIGN {
tuple val(meta), path("*fq.gz") , optional:true, emit: unmapped
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -15,6 +15,9 @@ process BISMARK_DEDUPLICATE {
tuple val(meta), path("*.deduplication_report.txt"), emit: report
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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@ -14,6 +14,9 @@ process BISMARK_GENOMEPREPARATION {
path "BismarkIndex" , emit: index
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

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@ -19,6 +19,9 @@ process BISMARK_METHYLATIONEXTRACTOR {
tuple val(meta), path("*.M-bias.txt") , emit: mbias
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def seqtype = meta.single_end ? '-s' : '-p'

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@ -14,6 +14,9 @@ process BISMARK_REPORT {
tuple val(meta), path("*report.{html,txt}"), emit: report
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -17,6 +17,9 @@ process BISMARK_SUMMARY {
path "*report.{html,txt}", emit: summary
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -15,6 +15,9 @@ process BLAST_BLASTN {
tuple val(meta), path('*.blastn.txt'), emit: txt
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process BLAST_MAKEBLASTDB {
path 'blast_db' , emit: db
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -17,6 +17,9 @@ process BOWTIE_ALIGN {
path "versions.yml" , emit: versions
tuple val(meta), path('*fastq.gz'), optional:true, emit: fastq
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: ''

View file

@ -14,6 +14,9 @@ process BOWTIE_BUILD {
path 'bowtie' , emit: index
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -18,6 +18,9 @@ process BOWTIE2_ALIGN {
tuple val(meta), path('*fastq.gz'), emit: fastq, optional:true
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: ''

View file

@ -14,6 +14,9 @@ process BOWTIE2_BUILD {
path 'bowtie2' , emit: index
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -15,6 +15,9 @@ process BWA_ALN {
tuple val(meta), path("*.sai"), emit: sai
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process BWA_INDEX {
path "bwa" , emit: index
path "versions.yml", emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -16,6 +16,9 @@ process BWA_MEM {
tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: ''

View file

@ -15,6 +15,9 @@ process BWA_SAMPE {
tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -15,6 +15,9 @@ process BWA_SAMSE {
tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process BWAMEM2_INDEX {
path "bwamem2" , emit: index
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -16,6 +16,9 @@ process BWAMEM2_MEM {
tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: ''

View file

@ -15,6 +15,9 @@ process BWAMETH_ALIGN {
tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: ''

View file

@ -14,6 +14,9 @@ process BWAMETH_INDEX {
path "bwameth" , emit: index
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -14,6 +14,9 @@ process CAT_CAT {
path "${file_out}*" , emit: file_out
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: ''

View file

@ -14,6 +14,9 @@ process CAT_FASTQ {
tuple val(meta), path("*.merged.fastq.gz"), emit: reads
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -1 +1,2 @@
cellranger-*.tar.gz
bcl2fastq2-*.zip

View file

@ -1,15 +1,22 @@
# Dockerfile to create container with Cell Ranger v6.1.2
# Push to nfcore/cellranger:<VER>
FROM continuumio/miniconda3:4.8.2
LABEL authors="Gisela Gabernet <gisela.gabernet@gmail.com>" \
description="Docker image containing Cell Ranger"
# Disclaimer: this container is not provided nor supported by 10x Genomics.
# Disclaimer: this container is not provided nor supported by Illumina or 10x Genomics.
# Install procps and clean apt cache
RUN apt-get update --allow-releaseinfo-change \
&& apt-get install -y procps \
&& apt-get install -y \
cpio \
procps \
rpm2cpio \
unzip \
&& apt-get clean -y && rm -rf /var/lib/apt/lists/*
# Copy pre-downloaded cellranger file
ENV CELLRANGER_VER 6.0.2
ENV CELLRANGER_VER=6.1.2
COPY cellranger-$CELLRANGER_VER.tar.gz /opt/cellranger-$CELLRANGER_VER.tar.gz
# Install cellranger

View file

@ -1,18 +1,24 @@
# Updating the docker container and making a new module release
Cell Ranger is a commercial tool by 10X Genomics. The container provided for the cellranger nf-core module is not provided nor supported by 10x Genomics. Updating the Cell Ranger version in the container and pushing the update to Dockerhub needs to be done manually.
Cell Ranger is a commercial tool from 10X Genomics. The container provided for the cellranger nf-core module is not provided nor supported by 10x Genomics. Updating the Cell Ranger versions in the container and pushing the update to Dockerhub needs to be done manually.
1. Navigate to the [Cell Ranger download page](https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest) and download the tar ball of the desired Cell Ranger version with `curl` or `wget`. Place this file in the same folder where the Dockerfile lies.
1. Navigate to the appropriate download page.
- [Cell Ranger](https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest): download the tar ball of the desired Cell Ranger version with `curl` or `wget`. Place this file in the same folder where the Dockerfile lies.
2. Edit the Dockerfile: update the Cell Ranger version in this line:
2. Edit the Dockerfile. Update the Cell Ranger versions in this line:
```bash
ENV CELLRANGER_VER <VERSION>
ENV CELLRANGER_VER=<VERSION>
```
3. Create the container:
3. Create and test the container:
```bash
docker build . -t nfcore/cellranger:<VERSION>
```
4. Access rights are needed to push the container to the Dockerhub nfcore organization, please ask a core team member to do so.
```bash
docker push nfcore/cellranger:<VERSION>
```

View file

@ -5,7 +5,7 @@ process CELLRANGER_COUNT {
if (params.enable_conda) {
exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers."
}
container "nfcore/cellranger:6.0.2"
container "nfcore/cellranger:6.1.2"
input:
tuple val(meta), path(reads)
@ -15,6 +15,9 @@ process CELLRANGER_COUNT {
path("sample-${meta.gem}/outs/*"), emit: outs
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def sample_arg = meta.samples.unique().join(",")

View file

@ -0,0 +1,40 @@
# Dockerfile to create container with Cell Ranger v6.1.2 and bcl2fastq v2.20.0
# Push to nfcore/cellrangermkfastq:<VER>
FROM continuumio/miniconda3:4.8.2
LABEL authors="Regina Reynolds, Gisela Gabernet <gisela.gabernet@gmail.com>" \
description="Docker image containing bcl2fastq2 and Cell Ranger"
# Disclaimer: this container is not provided nor supported by Illumina or 10x Genomics.
# Install procps and clean apt cache
RUN apt-get update --allow-releaseinfo-change \
&& apt-get install -y \
cpio \
procps \
rpm2cpio \
unzip \
&& apt-get clean -y && rm -rf /var/lib/apt/lists/*
# Copy pre-downloaded bcl2fastq2 and cellranger file
ENV BCL2FASTQ2_VER=v2-20-0-linux-x86-64 \
CELLRANGER_VER=6.1.2
COPY bcl2fastq2-$BCL2FASTQ2_VER.zip /tmp/bcl2fastq2-$BCL2FASTQ2_VER.zip
COPY cellranger-$CELLRANGER_VER.tar.gz /opt/cellranger-$CELLRANGER_VER.tar.gz
# Install bcl2fastq2
RUN \
cd /tmp && \
unzip bcl2fastq2-$BCL2FASTQ2_VER.zip && \
mv *.rpm bcl2fastq2-$BCL2FASTQ2_VER.rpm && \
rpm2cpio ./bcl2fastq2-$BCL2FASTQ2_VER.rpm | cpio -idmv && \
export PATH=/tmp/usr/local/bin/:$PATH && \
ln -s /tmp/usr/local/bin/bcl2fastq /usr/bin/bcl2fastq && \
rm -rf bcl2fastq2-$BCL2FASTQ2_VER.*
# Install cellranger
RUN \
cd /opt && \
tar -xzvf cellranger-$CELLRANGER_VER.tar.gz && \
export PATH=/opt/cellranger-$CELLRANGER_VER:$PATH && \
ln -s /opt/cellranger-$CELLRANGER_VER/cellranger /usr/bin/cellranger && \
rm -rf /opt/cellranger-$CELLRANGER_VER.tar.gz

View file

@ -0,0 +1,26 @@
# Updating the docker container and making a new module release
Bcl2fastq2 and Cell Ranger are commercial tools from Illumina and 10X Genomics, respectively. The container provided for the cellranger nf-core module is not provided nor supported by either Illumina or 10x Genomics. Updating the bcl2fastq2 or Cell Ranger versions in the container and pushing the update to Dockerhub needs to be done manually.
1. Navigate to the appropriate download pages.
- [bcl2fastq2](https://emea.support.illumina.com/sequencing/sequencing_software/bcl2fastq-conversion-software.html): download the linux rpm installer of the desired bcl2fastq2 version with `curl` or `wget`. Place this file in the same folder where the Dockerfile lies.
- [Cell Ranger](https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest): download the tar ball of the desired Cell Ranger version with `curl` or `wget`. Place this file in the same folder where the Dockerfile lies.
2. Edit the Dockerfile. Update the bcl2fastq2 and Cell Ranger versions in this line:
```bash
ENV BCL2FASTQ2_VER=<VERSION> \
CELLRANGER_VER=<VERSION>
```
3. Create and test the container:
```bash
docker build . -t nfcore/cellrangermkfastq:<CELLRANGER_VERSION>
```
4. Access rights are needed to push the container to the Dockerhub nfcore organization, please ask a core team member to do so.
```bash
docker push nfcore/cellrangermkfastq:<CELLRANGER_VERSION>
```

View file

@ -5,7 +5,7 @@ process CELLRANGER_MKFASTQ {
if (params.enable_conda) {
exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers."
}
container "litd/docker-cellranger:v6.1.1" // FIXME Add bcl2fastq to nf-core docker image
container "nfcore/cellrangermkfastq:6.1.2"
input:
path bcl
@ -13,14 +13,17 @@ process CELLRANGER_MKFASTQ {
output:
path "versions.yml", emit: versions
path "*.fastq.gz" , emit: fastq
path "${bcl.getSimpleName()}/outs/fastq_path/*.fastq.gz" , emit: fastq
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""
cellranger mkfastq --id=${bcl.getSimpleName()} \
--run=$bcl \
--csv=$csv
--csv=$csv \
$args
cat <<-END_VERSIONS > versions.yml
@ -28,4 +31,15 @@ process CELLRANGER_MKFASTQ {
cellranger: \$(echo \$( cellranger --version 2>&1) | sed 's/^.*[^0-9]\\([0-9]*\\.[0-9]*\\.[0-9]*\\).*\$/\\1/' )
END_VERSIONS
"""
stub:
"""
mkdir -p "${bcl.getSimpleName()}/outs/fastq_path/"
touch ${bcl.getSimpleName()}/outs/fastq_path/fake_file.fastq.gz
cat <<-END_VERSIONS > versions.yml
"${task.process}":
cellranger: \$(echo \$( cellranger --version 2>&1) | sed 's/^.*[^0-9]\\([0-9]*\\.[0-9]*\\.[0-9]*\\).*\$/\\1/' )
END_VERSIONS
"""
}

View file

@ -5,7 +5,7 @@ process CELLRANGER_MKGTF {
if (params.enable_conda) {
exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers."
}
container "nfcore/cellranger:6.0.2"
container "nfcore/cellranger:6.1.2"
input:
path gtf
@ -14,6 +14,9 @@ process CELLRANGER_MKGTF {
path "*.filtered.gtf", emit: gtf
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -5,7 +5,7 @@ process CELLRANGER_MKREF {
if (params.enable_conda) {
exit 1, "Conda environments cannot be used when using the Cell Ranger tool. Please use docker or singularity containers."
}
container "nfcore/cellranger:6.0.2"
container "nfcore/cellranger:6.1.2"
input:
path fasta
@ -16,6 +16,9 @@ process CELLRANGER_MKREF {
path "${reference_name}", emit: reference
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -16,6 +16,9 @@ process CHECKM_LINEAGEWF {
tuple val(meta), path("${prefix}.tsv"), emit: checkm_tsv
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -23,6 +23,9 @@ process CHROMAP_CHROMAP {
tuple val(meta), path("*.pairs.gz") , optional:true, emit: pairs
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def args2 = task.ext.args2 ?: ''

View file

@ -15,6 +15,9 @@ process CHROMAP_INDEX {
path "*.index" , emit: index
path "versions.yml", emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = fasta.baseName

View file

@ -19,6 +19,9 @@ process CLONALFRAMEML {
tuple val(meta), path("*.position_cross_reference.txt"), emit: pos_ref
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -16,6 +16,9 @@ process CMSEQ_POLYMUT {
tuple val(meta), path("*.txt"), emit: polymut
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -20,6 +20,9 @@ process CNVKIT_BATCH {
tuple val(meta), path("*.cns"), emit: cns, optional: true
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def normal_args = normal ? "--normal $normal" : ""

View file

@ -16,6 +16,9 @@ process COOLER_CLOAD {
tuple val(meta), val(cool_bin), path("*.cool"), emit: cool
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -16,6 +16,9 @@ process COOLER_DIGEST {
path "*.bed" , emit: bed
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -15,6 +15,9 @@ process COOLER_DUMP {
tuple val(meta), path("*.bedpe"), emit: bedpe
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process COOLER_MERGE {
tuple val(meta), path("*.cool"), emit: cool
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -14,6 +14,9 @@ process COOLER_ZOOMIFY {
tuple val(meta), path("*.mcool"), emit: mcool
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -16,6 +16,9 @@ process CSVTK_CONCAT {
tuple val(meta), path("${prefix}.${out_extension}"), emit: csv
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -16,6 +16,9 @@ process CSVTK_SPLIT {
tuple val(meta), path("*.${out_extension}"), emit: split_csv
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -15,6 +15,9 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
path "software_versions_mqc.yml", emit: mqc_yml
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
template 'dumpsoftwareversions.py'

View file

@ -15,6 +15,9 @@ process CUSTOM_GETCHROMSIZES {
path '*.fai' , emit: fai
path "versions.yml", emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
"""

View file

@ -15,6 +15,9 @@ process CUTADAPT {
tuple val(meta), path('*.log') , emit: log
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -17,6 +17,9 @@ process DAMAGEPROFILER {
tuple val(meta), path("${prefix}"), emit: results
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}"

View file

@ -26,6 +26,9 @@ process DASTOOL_DASTOOL {
tuple val(meta), path("*.seqlength") , optional: true, emit: seqlength
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"

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