Add deeptools

This commit is contained in:
drpatelh 2020-08-05 17:17:34 +01:00
parent f481899049
commit 9d168cf29e
8 changed files with 164 additions and 0 deletions

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../../lib/functions.nf

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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
process DEEPTOOLS_COMPUTEMATRIX {
tag "$meta.id"
label 'process_high'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
container "quay.io/biocontainers/deeptools:3.4.3--py_0"
//container "https://depot.galaxyproject.org/singularity/deeptools:3.4.3--py_0"
conda (params.conda ? "bioconda::deeptools=3.4.3" : null)
input:
tuple val(meta), path(bigwig)
path bed
val options
output:
tuple val(meta), path("*.mat.gz"), emit: matrix
tuple val(meta), path("*.mat.tab"), emit: table
path "*.version.txt", emit: version
script:
def software = getSoftwareName(task.process)
def ioptions = initOptions(options)
def prefix = ioptions.suffix ? "${meta.id}${ioptions.suffix}" : "${meta.id}"
"""
computeMatrix \\
$ioptions.args \\
--regionsFileName $bed \\
--scoreFileName $bigwig \\
--outFileName ${prefix}.computeMatrix.mat.gz \\
--outFileNameMatrix ${prefix}.computeMatrix.vals.mat.tab \\
--numberOfProcessors $task.cpus
computeMatrix --version | sed -e "s/computeMatrix //g" > ${software}.version.txt
"""
}

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../../lib/functions.nf

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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
process DEEPTOOLS_PLOTFINGERPRINT {
tag "$meta.id"
label 'process_high'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
container "quay.io/biocontainers/deeptools:3.4.3--py_0"
//container "https://depot.galaxyproject.org/singularity/deeptools:3.4.3--py_0"
conda (params.conda ? "bioconda::deeptools=3.4.3" : null)
input:
tuple val(meta), path(bams), path(bais)
val options
output:
tuple val(meta), path("*.pdf"), emit: pdf
tuple val(meta), path("*.raw.txt"), emit: matrix
tuple val(meta), path("*.qcmetrics.txt"), emit: metrics
path "*.version.txt", emit: version
script:
def software = getSoftwareName(task.process)
def ioptions = initOptions(options)
def prefix = ioptions.suffix ? "${meta.id}${ioptions.suffix}" : "${meta.id}"
def extend = (meta.single_end && params.fragment_size > 0) ? "--extendReads ${params.fragment_size}" : ''
"""
plotFingerprint \\
$ioptions.args \\
$extend \\
--bamfiles ${bams.join(' ')} \\
--plotFile ${prefix}.plotFingerprint.pdf \\
--outRawCounts ${prefix}.plotFingerprint.raw.txt \\
--outQualityMetrics ${prefix}.plotFingerprint.qcmetrics.txt \\
--numberOfProcessors $task.cpus
plotFingerprint --version | sed -e "s/plotFingerprint //g" > ${software}.version.txt
"""
}

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../../lib/functions.nf

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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
process DEEPTOOLS_PLOTHEATMAP {
tag "$meta.id"
label 'process_low'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
container "quay.io/biocontainers/deeptools:3.4.3--py_0"
//container "https://depot.galaxyproject.org/singularity/deeptools:3.4.3--py_0"
conda (params.conda ? "bioconda::deeptools=3.4.3" : null)
input:
tuple val(meta), path(matrix)
val options
output:
tuple val(meta), path("*.pdf"), emit: pdf
tuple val(meta), path("*.tab"), emit: table
path "*.version.txt", emit: version
script:
def software = getSoftwareName(task.process)
def ioptions = initOptions(options)
def prefix = ioptions.suffix ? "${meta.id}${ioptions.suffix}" : "${meta.id}"
"""
plotHeatmap \\
$ioptions.args \\
--matrixFile $matrix \\
--outFileName ${prefix}.plotHeatmap.pdf \\
--outFileNameMatrix ${prefix}.plotHeatmap.mat.tab
plotHeatmap --version | sed -e "s/plotHeatmap //g" > ${software}.version.txt
"""
}

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../../lib/functions.nf

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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
process DEEPTOOLS_PLOTPROFILE {
tag "$meta.id"
label 'process_low'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
container "quay.io/biocontainers/deeptools:3.4.3--py_0"
//container "https://depot.galaxyproject.org/singularity/deeptools:3.4.3--py_0"
conda (params.conda ? "bioconda::deeptools=3.4.3" : null)
input:
tuple val(meta), path(matrix)
val options
output:
tuple val(meta), path("*.pdf"), emit: pdf
tuple val(meta), path("*.tab"), emit: table
path "*.version.txt", emit: version
script:
def software = getSoftwareName(task.process)
def ioptions = initOptions(options)
prefix = ioptions.suffix ? "${meta.id}${ioptions.suffix}" : "${meta.id}"
"""
plotProfile \\
$ioptions.args \\
--matrixFile $matrix \\
--outFileName ${prefix}.plotProfile.pdf \\
--outFileNameData ${prefix}.plotProfile.tab
plotProfile --version | sed -e "s/plotProfile //g" > ${software}.version.txt
"""
}