diff --git a/.github/filters.yml b/.github/filters.yml
index 695ac1b9..b38837b7 100644
--- a/.github/filters.yml
+++ b/.github/filters.yml
@@ -225,6 +225,10 @@ seacr_callpeak:
   - software/seacr/callpeak/**
   - tests/software/seacr/callpeak/**
 
+seqkit_split2:
+  - software/seqkit/split2/**
+  - tests/software/seqkit/split2/**
+
 star_align:
   - software/star/align/**
   - tests/software/star/align/**