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Update module according to discussion on Slack
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parent
01a926866e
commit
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3 changed files with 45 additions and 19 deletions
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@ -1,29 +1,40 @@
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nextflow.preview.dsl = 2
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def MODULE = "fastqc"
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def MODULE = "fastqc"
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params.publish_dir = MODULE
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params.publish_dir = MODULE
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params.publish_results = "default"
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params.publish_results = "default"
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process FASTQC {
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process FASTQC {
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input:
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publishDir "${params.out_dir}/${params.publish_dir}",
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tuple val(name), path(reads)
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output:
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tuple val(name), path ("*fastqc*"), emit: all
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path "*.zip", emit: report // e.g. for MultiQC later
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path "*.version.txt", emit: version
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container "docker.pkg.github.com/nf-core/$MODULE"
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conda "${moduleDir}/environment.yml"
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publishDir "${params.out_dir}/${params.publish_dir}/$name",
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mode: params.publish_dir_mode,
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mode: params.publish_dir_mode,
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saveAs: { filename ->
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saveAs: { filename ->
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if (params.publish_results == "none") null
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if (params.publish_results == "none") null
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else filename }
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else filename }
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container "docker.pkg.github.com/nf-core/$MODULE"
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conda "${moduleDir}/environment.yml"
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input:
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tuple val(name), val(single_end), path(reads)
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output:
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tuple val(name), val(single_end), path("*.html"), emit: html
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tuple val(name), val(single_end), path("*.zip"), emit: zip
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path "*.version.txt", emit: version
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script:
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script:
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// Add soft-links to original FastQs for consistent naming in pipeline
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if (single_end) {
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"""
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"""
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fastqc ${params.fastqc_args} -t ${task.cpus} $reads
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[ ! -f ${name}.fastq.gz ] && ln -s $reads ${name}.fastq.gz
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fastqc ${params.fastqc_args} --threads $task.cpus ${name}.fastq.gz
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fastqc --version | sed -n "s/.*\\(v.*\$\\)/\\1/p" > fastqc.version.txt
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"""
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} else {
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"""
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[ ! -f ${name}_1.fastq.gz ] && ln -s ${reads[0]} ${name}_1.fastq.gz
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[ ! -f ${name}_2.fastq.gz ] && ln -s ${reads[1]} ${name}_2.fastq.gz
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fastqc ${params.fastqc_args} --threads $task.cpus ${name}_1.fastq.gz ${name}_2.fastq.gz
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fastqc --version | sed -n "s/.*\\(v.*\$\\)/\\1/p" > fastqc.version.txt
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fastqc --version | sed -n "s/.*\\(v.*\$\\)/\\1/p" > fastqc.version.txt
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"""
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"""
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}
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}
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}
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@ -29,14 +29,28 @@ params:
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Append to the path for the standard output directory provided by `$out_dir`.
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Append to the path for the standard output directory provided by `$out_dir`.
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- publish_dir_mode:
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- publish_dir_mode:
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type: string
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type: string
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description: Provide a value for the Nextflow `publishDir` mode parameter (e.g. copy, link, ...)
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description: |
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Provide a value for the Nextflow `publishDir` mode parameter
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(e.g. copy, link, ...)
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- publish_results:
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type: string
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description: |
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Whether or not to publish results into `publish_dir`. Set to `none` to not
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publish any files at all; to `default` to publish all relevant files.
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input:
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input:
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- name:
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- name:
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type: string
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type: string
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description: Sample identifier
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description: Sample identifier
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- single_end:
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type: boolean
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description: |
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Boolean indicating whether the corresponding sample is single-end (true)
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or paired-end (false).
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- reads:
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- reads:
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type: file
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type: file
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description: List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.
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description: |
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List of input FastQ files of size 1 and 2 for single-end and paired-end data,
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respectively.
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output:
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output:
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- report:
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- report:
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type: file
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type: file
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@ -12,7 +12,7 @@ include { FASTQC } from '../main.nf'
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*/
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*/
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workflow test_single_end {
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workflow test_single_end {
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input_files = Channel.fromPath("data/test_single_end.fastq.gz")
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input_files = Channel.fromPath("data/test_single_end.fastq.gz")
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.map {f -> [f.baseName, f]}
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.map {f -> [f.baseName, true, f]}
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FASTQC(input_files)
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FASTQC(input_files)
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}
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}
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@ -21,6 +21,7 @@ workflow test_single_end {
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*/
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*/
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workflow test_paired_end {
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workflow test_paired_end {
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input_files = Channel.fromFilePairs("data/test_R{1,2}.fastq.gz")
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input_files = Channel.fromFilePairs("data/test_R{1,2}.fastq.gz")
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.map {f -> [f[0], false, f[1]]}
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FASTQC(input_files)
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FASTQC(input_files)
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}
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}
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