Mtnucratio (#986)

* add new module samtools_depth

* fixed main.nf for samtools/depth

* add new module mtnucratio

* fix main.nf

* Apply suggestions from code review

Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>

* Apply suggestions from code review

Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>

* layout in main.nf

Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>

modules/mtnucratio/functions.nf

@@ -0,0 +1,78 @@
+//
+//  Utility functions used in nf-core DSL2 module files
+//
+
+//
+// Extract name of software tool from process name using $task.process
+//
+def getSoftwareName(task_process) {
+    return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()
+}
+
+//
+// Extract name of module from process name using $task.process
+//
+def getProcessName(task_process) {
+    return task_process.tokenize(':')[-1]
+}
+
+//
+// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules
+//
+def initOptions(Map args) {
+    def Map options = [:]
+    options.args            = args.args ?: ''
+    options.args2           = args.args2 ?: ''
+    options.args3           = args.args3 ?: ''
+    options.publish_by_meta = args.publish_by_meta ?: []
+    options.publish_dir     = args.publish_dir ?: ''
+    options.publish_files   = args.publish_files
+    options.suffix          = args.suffix ?: ''
+    return options
+}
+
+//
+// Tidy up and join elements of a list to return a path string
+//
+def getPathFromList(path_list) {
+    def paths = path_list.findAll { item -> !item?.trim().isEmpty() }      // Remove empty entries
+    paths     = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes
+    return paths.join('/')
+}
+
+//
+// Function to save/publish module results
+//
+def saveFiles(Map args) {
+    def ioptions  = initOptions(args.options)
+    def path_list = [ ioptions.publish_dir ?: args.publish_dir ]
+
+    // Do not publish versions.yml unless running from pytest workflow
+    if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) {
+        return null
+    }
+    if (ioptions.publish_by_meta) {
+        def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta
+        for (key in key_list) {
+            if (args.meta && key instanceof String) {
+                def path = key
+                if (args.meta.containsKey(key)) {
+                    path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key]
+                }
+                path = path instanceof String ? path : ''
+                path_list.add(path)
+            }
+        }
+    }
+    if (ioptions.publish_files instanceof Map) {
+        for (ext in ioptions.publish_files) {
+            if (args.filename.endsWith(ext.key)) {
+                def ext_list = path_list.collect()
+                ext_list.add(ext.value)
+                return "${getPathFromList(ext_list)}/$args.filename"
+            }
+        }
+    } else if (ioptions.publish_files == null) {
+        return "${getPathFromList(path_list)}/$args.filename"
+    }
+}

modules/mtnucratio/main.nf

@@ -0,0 +1,43 @@
+include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions'
+
+params.options = [:]
+options        = initOptions(params.options)
+
+process MTNUCRATIO {
+    tag "$meta.id"
+    label 'process_low'
+    publishDir "${params.outdir}",
+        mode: params.publish_dir_mode,
+        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
+
+    conda (params.enable_conda ? "bioconda::mtnucratio=0.7" : null)
+    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
+        container "https://depot.galaxyproject.org/singularity/mtnucratio:0.7--hdfd78af_2"
+    } else {
+        container "quay.io/biocontainers/mtnucratio:0.7--hdfd78af_2"
+    }
+
+    input:
+    tuple val(meta), path(bam)
+    val(mt_id)
+
+    output:
+    tuple val(meta), path("*.mtnucratio"), emit: mtnucratio
+    tuple val(meta), path("*.json")      , emit: json
+    path "versions.yml"                  , emit: versions
+
+    script:
+    def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
+
+    """
+    mtnucratio \\
+        $options.args \\
+        $bam \\
+        $mt_id
+
+    cat <<-END_VERSIONS > versions.yml
+    ${getProcessName(task.process)}:
+        ${getSoftwareName(task.process)}: \$(echo \$(mtnucratio --version 2>&1) | head -n1 | sed 's/Version: //')
+    END_VERSIONS
+    """
+}

modules/mtnucratio/meta.yml

@@ -0,0 +1,54 @@
+name: mtnucratio
+description: A small Java tool to calculate ratios between MT and nuclear sequencing reads in a given BAM file.
+keywords:
+  - mtnucratio
+  - ratio
+  - reads
+  - bam
+  - mitochondrial to nuclear ratio
+  - mitochondria
+  - statistics
+tools:
+  - mtnucratio:
+      description: A small tool to determine MT to Nuclear ratios for NGS data.
+      homepage: https://github.com/apeltzer/MTNucRatioCalculator
+      documentation: https://github.com/apeltzer/MTNucRatioCalculator
+      tool_dev_url: https://github.com/apeltzer/MTNucRatioCalculator
+      doi: "10.1186/s13059-016-0918-z"
+      licence: ['GPL v3']
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: (coordinate) sorted BAM/SAM file
+      pattern: "*.{bam,sam}"
+  - mt_id:
+      type: string
+      description: Identifier of the contig/chromosome of interest (e.g. chromosome, contig) as in the aligned against reference FASTA file, e.g. mt or chrMT for mitochondria
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - mtnucratio:
+      type: file
+      description: Text file containing metrics (mtreads, mt_cov_avg, nucreads, nuc_cov_avg, mt_nuc_ratio)
+      pattern: "*.mtnucratio"
+  - json:
+      type: file
+      description: JSON file, containing metadata map with sample name, tool name and version, and metrics as in txt file
+      pattern: "*.json"
+
+authors:
+  - "@louperelo"

tests/config/pytest_modules.yml

@@ -778,6 +778,10 @@ msisensor/scan:
   - modules/msisensor/scan/**
   - tests/modules/msisensor/scan/**
 
+mtnucratio:
+  - modules/mtnucratio/**
+  - tests/modules/mtnucratio/**
+
 multiqc:
   - modules/fastqc/**
   - modules/multiqc/**

tests/modules/mtnucratio/main.nf

@@ -0,0 +1,14 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { MTNUCRATIO } from '../../../modules/mtnucratio/main.nf' addParams( options: [:] )
+
+workflow test_mtnucratio {
+    
+    input = [ [ id:'test', single_end:false ], // meta map
+              file(params.test_data['sarscov2']['illumina']['test_single_end_sorted_bam'], checkIfExists: true)]
+    mt_id = 'mt_id'
+
+    MTNUCRATIO ( input, mt_id )
+}

tests/modules/mtnucratio/test.yml

@@ -0,0 +1,9 @@
+- name: mtnucratio
+  command: nextflow run tests/modules/mtnucratio -entry test_mtnucratio -c tests/config/nextflow.config
+  tags:
+    - mtnucratio
+  files:
+    - path: output/mtnucratio/test.single_end.sorted.bam.mtnucratio
+      md5sum: 19e96849802c70aa0694785f716274b7
+    - path: output/mtnucratio/test.single_end.sorted.bam.mtnucratiomtnuc.json
+      md5sum: 14d24be6272854d6762f0dfad5918ef6