Seqtk rename (#1304)

* Added seqtk/rename module and tests code

* Updated files and testing code for seqtk rename

* Added meta map to seqtk/rename module def

* updated prefix parameter usage

* updated test.yml to remove local filepaths --> change to output

* Added empty line to main.nf
This commit is contained in:
Hunter Seabolt 2022-02-16 11:28:08 -05:00 committed by GitHub
parent 7560689375
commit a69faefee8
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6 changed files with 131 additions and 0 deletions

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@ -0,0 +1,40 @@
process SEQTK_RENAME {
tag "$meta.id"
label 'process_low'
conda (params.enable_conda ? "bioconda::seqtk=1.3" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/seqtk:1.3--h5bf99c6_3' :
'quay.io/biocontainers/seqtk:1.3--h5bf99c6_3' }"
input:
tuple val(meta), path(sequences)
output:
tuple val(meta), path("*.gz") , emit: sequences
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def extension = "fasta"
if ("$sequences" ==~ /.+\.fq|.+\.fq.gz|.+\.fastq|.+\.fastq.gz/) {
extension = "fastq"
}
"""
seqtk \\
rename \\
$args \\
$sequences \\
$prefix | \\
gzip -c --no-name > ${prefix}.renamed.${extension}.gz
cat <<-END_VERSIONS > versions.yml
"${task.process}":
seqtk: \$(echo \$(seqtk 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
END_VERSIONS
"""
}

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@ -0,0 +1,42 @@
name: seqtk_rename
description: Rename sequence names in FASTQ or FASTA files.
keywords:
- rename
tools:
- seqtk:
description: Seqtk is a fast and lightweight tool for processing sequences in the FASTA or FASTQ format. The seqtk rename command renames sequence names.
homepage: https://github.com/lh3/seqtk
documentation: https://docs.csc.fi/apps/seqtk/
tool_dev_url: https://github.com/lh3/seqtk
licence: ['MIT']
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test' ]
- sequences:
type: file
description: A FASTQ or FASTA file
pattern: "*.{fastq.gz, fastq, fq, fq.gz, fasta, fastq.gz, fa, fa.gz, fas, fas.gz, fna, fna.gz}"
output:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test' ]
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
- sequences:
type: file
description: FASTQ/FASTA file containing renamed sequences
pattern: "*.{fastq.gz, fasta.gz}"
authors:
- "@hseabolt"
- "@mjcipriano"
- "@sateeshperi"

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@ -1377,6 +1377,10 @@ seqtk/mergepe:
- modules/seqtk/mergepe/**
- tests/modules/seqtk/mergepe/**
seqtk/rename:
- modules/seqtk/rename/**
- tests/modules/seqtk/rename/**
seqtk/sample:
- modules/seqtk/sample/**
- tests/modules/seqtk/sample/**

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@ -0,0 +1,19 @@
#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
include { SEQTK_RENAME } from '../../../../modules/seqtk/rename/main.nf'
workflow test_seqtk_rename {
input = [ [ id:'test' ], // meta map
[ file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ]
]
SEQTK_RENAME ( input )
}
workflow test_seqtk_rename_fq {
input = [ [ id:'test' ], // meta map
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
]
SEQTK_RENAME ( input )
}

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@ -0,0 +1,5 @@
process {
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
}

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@ -0,0 +1,21 @@
- name: seqtk rename test_seqtk_rename
command: nextflow run tests/modules/seqtk/rename -entry test_seqtk_rename -c tests/config/nextflow.config
tags:
- seqtk
- seqtk/rename
files:
- path: output/seqtk/test.renamed.fasta.gz
md5sum: 7b407952dcf0d925f1996e04a201d05b
- path: output/seqtk/versions.yml
md5sum: 24127592f1b9e5ee8e5ab04ee748c491
- name: seqtk rename test_seqtk_rename_fq
command: nextflow run tests/modules/seqtk/rename -entry test_seqtk_rename_fq -c tests/config/nextflow.config
tags:
- seqtk
- seqtk/rename
files:
- path: output/seqtk/test.renamed.fastq.gz
md5sum: babdfc2a3940a1e32a63479db2c1d600
- path: output/seqtk/versions.yml
md5sum: 06c19670eb2b4185e8f4fa5dcf8fb0d5