diff --git a/modules/gatk/unifiedgenotyper/main.nf b/modules/gatk/unifiedgenotyper/main.nf new file mode 100644 index 00000000..e88ac7c5 --- /dev/null +++ b/modules/gatk/unifiedgenotyper/main.nf @@ -0,0 +1,63 @@ +process GATK_UNIFIEDGENOTYPER { + tag "$meta.id" + label 'process_medium' + + conda (params.enable_conda ? "bioconda::gatk=3.5" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk:3.5--hdfd78af_11': + 'quay.io/biocontainers/gatk:3.5--hdfd78af_11' }" + + input: + tuple val(meta), path(input), path(index) + path fasta + path fai + path dict + path intervals + path contamination + path dbsnp + path comp + + output: + tuple val(meta), path("*.vcf.gz"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def contamination_file = contamination ? "-contaminationFile ${contamination}" : "" + def dbsnp_file = dbsnp ? "--dbsnp ${dbsnp}" : "" + def comp_file = comp ? "--comp ${comp}" : "" + def intervals_file = intervals ? "--intervals ${intervals}" : "" + + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK RealignerTargetCreator] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + + """ + gatk3 \\ + -Xmx${avail_mem}g \\ + -nt ${task.cpus} \\ + -T UnifiedGenotyper \\ + -I ${input} \\ + -R ${fasta} \\ + ${contamination_file} \\ + ${dbsnp_file} \\ + ${comp_file} \\ + ${intervals_file} \\ + -o ${prefix}.vcf \\ + $args + + gzip -n *.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk: \$(echo \$(gatk3 --version)) + END_VERSIONS + """ +} diff --git a/modules/gatk/unifiedgenotyper/meta.yml b/modules/gatk/unifiedgenotyper/meta.yml new file mode 100644 index 00000000..e414d146 --- /dev/null +++ b/modules/gatk/unifiedgenotyper/meta.yml @@ -0,0 +1,73 @@ +name: "gatk_unifiedgenotyper" +keywords: + - bam + - vcf + - variant calling +tools: + - "gatk": + description: "The full Genome Analysis Toolkit (GATK) framework, license restricted." + homepage: "https://gatk.broadinstitute.org/hc/en-us" + documentation: "https://github.com/broadinstitute/gatk-docs" + licence: "['https://software.broadinstitute.org/gatk/download/licensing', 'BSD', 'https://www.broadinstitute.org/gatk/about/#licensing']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input: + type: file + description: Sorted and indexed BAM/CRAM/SAM file + pattern: "*.bam" + - index: + type: file + description: BAM index file + pattern: "*.bai" + - fasta: + type: file + description: Reference file used to generate BAM file + pattern: ".{fasta,fa,fna}" + - fai: + type: file + description: Index of reference file used to generate BAM file + pattern: ".fai" + - dict: + type: file + description: GATK dict file for reference + pattern: ".dict" + - intervals: + type: file + description: Bed file with the genomic regions included in the library (optional) + pattern: "*.intervals" + - contamination: + type: file + description: Tab-separated file containing fraction of contamination in sequencing data (per sample) to aggressively remove + pattern: "*" + - dbsnps: + type: file + description: VCF file containing known sites (optional) + pattern: "*" + - comp: + type: file + description: Comparison VCF file (optional) + pattern: "*" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: VCF file containing called variants + pattern: "*.vcf.gz" + +authors: + - "@ilight1542" + - "@jfy133" diff --git a/tests/config/pytest_modules.yml b/tests/config/pytest_modules.yml index 04580c79..715f7905 100644 --- a/tests/config/pytest_modules.yml +++ b/tests/config/pytest_modules.yml @@ -715,6 +715,10 @@ gamma/gamma: - modules/gamma/gamma/** - tests/modules/gamma/gamma/** +gatk/unifiedgenotyper: + - modules/gatk/unifiedgenotyper/** + - tests/modules/gatk/unifiedgenotyper/** + gatk4/applybqsr: - modules/gatk4/applybqsr/** - tests/modules/gatk4/applybqsr/** diff --git a/tests/modules/gatk/unifiedgenotyper/main.nf b/tests/modules/gatk/unifiedgenotyper/main.nf new file mode 100644 index 00000000..6d145e76 --- /dev/null +++ b/tests/modules/gatk/unifiedgenotyper/main.nf @@ -0,0 +1,18 @@ +#!/usr/bin/env nextflow + +nextflow.enable.dsl = 2 + +include { GATK_UNIFIEDGENOTYPER } from '../../../../modules/gatk/unifiedgenotyper/main.nf' + +workflow test_gatk_unifiedgenotyper { + + input = [ [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), + ] + fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + dict = file(params.test_data['sarscov2']['genome']['genome_dict'], checkIfExists: true) + + GATK_UNIFIEDGENOTYPER ( input, fasta, fai, dict, [], [], [], []) +} diff --git a/tests/modules/gatk/unifiedgenotyper/nextflow.config b/tests/modules/gatk/unifiedgenotyper/nextflow.config new file mode 100644 index 00000000..50f50a7a --- /dev/null +++ b/tests/modules/gatk/unifiedgenotyper/nextflow.config @@ -0,0 +1,5 @@ +process { + + publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } + +} \ No newline at end of file diff --git a/tests/modules/gatk/unifiedgenotyper/test.yml b/tests/modules/gatk/unifiedgenotyper/test.yml new file mode 100644 index 00000000..498e7991 --- /dev/null +++ b/tests/modules/gatk/unifiedgenotyper/test.yml @@ -0,0 +1,9 @@ +- name: gatk unifiedgenotyper test_gatk_unifiedgenotyper + command: nextflow run ./tests/modules/gatk/unifiedgenotyper -entry test_gatk_unifiedgenotyper -c ./tests/config/nextflow.config -c ./tests/modules/gatk/unifiedgenotyper/nextflow.config + tags: + - gatk + - gatk/unifiedgenotyper + files: + - path: output/gatk/test.vcf.gz + contains: + - "#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT test"