mirror of
https://github.com/MillironX/nf-core_modules.git
synced 2024-12-22 19:18:17 +00:00
parent
601bf5eb64
commit
b3278a1e63
42 changed files with 327 additions and 237 deletions
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@ -2,7 +2,7 @@
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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params.options = [:]
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params.options = [:]
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def options = initOptions(params.options)
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options = initOptions(params.options)
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process CNVKIT {
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process CNVKIT {
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tag "$meta.id"
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tag "$meta.id"
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@ -20,8 +20,8 @@ process CNVKIT {
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input:
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input:
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tuple val(meta), path(tumourbam), path(normalbam)
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tuple val(meta), path(tumourbam), path(normalbam)
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path fasta
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path fasta
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path targetfile
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path targetfile
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output:
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output:
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tuple val(meta), path("*.bed"), emit: bed
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tuple val(meta), path("*.bed"), emit: bed
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@ -32,15 +32,15 @@ process CNVKIT {
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script:
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script:
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def software = getSoftwareName(task.process)
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
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"""
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"""
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cnvkit.py batch \\
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cnvkit.py \\
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batch \\
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$tumourbam \\
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$tumourbam \\
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--normal $normalbam\\
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--normal $normalbam\\
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--fasta $fasta \\
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--fasta $fasta \\
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--targets $targetfile \\
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--targets $targetfile \\
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$options.args
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$options.args
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cnvkit.py version | sed -e "s/cnvkit v//g" > ${software}.version.txt
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echo \$(cnvkit.py version) | sed -e "s/cnvkit v//g" > ${software}.version.txt
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"""
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"""
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}
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}
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@ -20,10 +20,10 @@ process GATK4_APPLYBQSR {
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input:
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input:
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tuple val(meta), path(bam), path(bai), path(bqsr_table)
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tuple val(meta), path(bam), path(bai), path(bqsr_table)
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path fasta
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path fasta
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path fastaidx
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path fastaidx
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path dict
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path dict
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path intervalsBed
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path intervals
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output:
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output:
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tuple val(meta), path("*.bam"), emit: bam
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tuple val(meta), path("*.bam"), emit: bam
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@ -32,17 +32,16 @@ process GATK4_APPLYBQSR {
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script:
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script:
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def software = getSoftwareName(task.process)
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : ""
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def interval = intervals ? "-L ${intervals}" : ""
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"""
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"""
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gatk ApplyBQSR \\
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gatk ApplyBQSR \\
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-R $fasta \\
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-R $fasta \\
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-I $bam \\
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-I $bam \\
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--bqsr-recal-file $bqsr_table \\
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--bqsr-recal-file $bqsr_table \\
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$intervalsCommand \\
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$interval \\
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-O ${prefix}.bam \\
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-O ${prefix}.bam \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -45,6 +45,6 @@ process GATK4_BASERECALIBRATOR {
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$options.args \
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$options.args \
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-O ${prefix}.table
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-O ${prefix}.table
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -28,7 +28,7 @@ process GATK4_BEDTOINTERVALLIST {
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script:
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script:
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def software = getSoftwareName(task.process)
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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"""
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"""
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gatk BedToIntervalList \\
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gatk BedToIntervalList \\
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-I $bed \\
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-I $bed \\
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@ -36,6 +36,6 @@ process GATK4_BEDTOINTERVALLIST {
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-O ${prefix}.interval_list \\
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-O ${prefix}.interval_list \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -40,6 +40,6 @@ process GATK4_CREATESEQUENCEDICTIONARY {
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--URI $fasta \\
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--URI $fasta \\
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$options.args
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$options.args
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echo \$(gatk CreateSequenceDictionary --version 2>&1) | sed 's/^.*(GATK) v//; s/ HTSJDK.*\$//' > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -35,6 +35,7 @@ process GATK4_FASTQTOSAM {
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-O ${prefix}.bam \\
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-O ${prefix}.bam \\
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-SM $prefix \\
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-SM $prefix \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -47,6 +47,6 @@ process GATK4_HAPLOTYPECALLER {
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-O ${prefix}.vcf.gz \\
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-O ${prefix}.vcf.gz \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -33,10 +33,10 @@ process GATK4_INTERVALLISTTOOLS {
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mkdir ${prefix}_split
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mkdir ${prefix}_split
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gatk \\
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gatk \\
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IntervalListTools \\
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IntervalListTools \\
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-I ${interval_list} \\
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-I ${interval_list} \\
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-O ${prefix}_split \\
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-O ${prefix}_split \\
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$options.args
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$options.args
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python3 <<CODE
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python3 <<CODE
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import glob, os
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import glob, os
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@ -48,6 +48,6 @@ process GATK4_INTERVALLISTTOOLS {
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os.rename(interval, newName)
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os.rename(interval, newName)
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CODE
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CODE
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -39,6 +39,6 @@ process GATK4_MARKDUPLICATES {
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--OUTPUT ${prefix}.bam \\
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--OUTPUT ${prefix}.bam \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -2,7 +2,7 @@
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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params.options = [:]
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params.options = [:]
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def options = initOptions(params.options)
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options = initOptions(params.options)
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process GATK4_MERGEBAMALIGNMENT {
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process GATK4_MERGEBAMALIGNMENT {
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tag "$meta.id"
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tag "$meta.id"
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@ -20,25 +20,25 @@ process GATK4_MERGEBAMALIGNMENT {
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input:
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input:
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tuple val(meta), path(aligned)
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tuple val(meta), path(aligned)
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path unmapped
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path unmapped
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path fasta
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path fasta
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path dict
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path dict
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output:
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output:
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tuple val(meta), path('*.merged.bam'), emit: bam
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tuple val(meta), path('*.bam'), emit: bam
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path '*.version.txt' , emit: version
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path '*.version.txt' , emit: version
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script:
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script:
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def software = getSoftwareName(task.process)
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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"""
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"""
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gatk MergeBamAlignment \\
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gatk MergeBamAlignment \\
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ALIGNED=$aligned \\
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ALIGNED=$aligned \\
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UNMAPPED=$unmapped \\
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UNMAPPED=$unmapped \\
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R=$fasta \\
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R=$fasta \\
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O=${prefix}.merged.bam \\
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O=${prefix}.bam \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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input:
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input:
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tuple val(meta), path(vcfs)
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tuple val(meta), path(vcfs)
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path(ref_dict)
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path ref_dict
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val use_ref_dict
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val use_ref_dict
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output:
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output:
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tuple val(meta), path('*.vcf.gz') , emit: vcf
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tuple val(meta), path('*.vcf.gz'), emit: vcf
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path '*.version.txt' , emit: version
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path '*.version.txt' , emit: version
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script:
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script:
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def software = getSoftwareName(task.process)
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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// Make list of VCFs to merge
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// Make list of VCFs to merge
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def input = ""
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def input = ""
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for (vcf in vcfs) {
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for (vcf in vcfs) {
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"""
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"""
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gatk MergeVcfs \\
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gatk MergeVcfs \\
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$input \\
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$input \\
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O=${prefix}.merged.vcf.gz \\
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O=${prefix}.vcf.gz \\
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$ref \\
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$ref \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -22,18 +22,18 @@ process GATK4_REVERTSAM {
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tuple val(meta), path(bam)
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tuple val(meta), path(bam)
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output:
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output:
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tuple val(meta), path('*.bam') , emit: bam
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tuple val(meta), path('*.bam'), emit: bam
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path '*.version.txt' , emit: version
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path '*.version.txt' , emit: version
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script:
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script:
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def software = getSoftwareName(task.process)
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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"""
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"""
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gatk RevertSam \\
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gatk RevertSam \\
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I=$bam \\
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I=$bam \\
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O=${prefix}.reverted.bam \\
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O=${prefix}.reverted.bam \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -22,19 +22,19 @@ process GATK4_SAMTOFASTQ {
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tuple val(meta), path(bam)
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tuple val(meta), path(bam)
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output:
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output:
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tuple val(meta), path('*.fastq.gz') , emit: fastq
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tuple val(meta), path('*.fastq.gz'), emit: fastq
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path '*.version.txt' , emit: version
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path '*.version.txt' , emit: version
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script:
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script:
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def software = getSoftwareName(task.process)
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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def output = meta.single_end ? "FASTQ=${prefix}.fastq.gz" : "FASTQ=${prefix}_1.fastq.gz SECOND_END_FASTQ=${prefix}_2.fastq.gz"
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def output = meta.single_end ? "FASTQ=${prefix}.fastq.gz" : "FASTQ=${prefix}_1.fastq.gz SECOND_END_FASTQ=${prefix}_2.fastq.gz"
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"""
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"""
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gatk SamToFastq \\
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gatk SamToFastq \\
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I=$bam \\
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I=$bam \\
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$output \\
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$output \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -23,19 +23,19 @@ process GATK4_SPLITNCIGARREADS {
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tuple path(fasta), path(fai), path(dict)
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tuple path(fasta), path(fai), path(dict)
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output:
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output:
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tuple val(meta), path('*.split_cigar.bam'), emit: bam
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tuple val(meta), path('*.bam'), emit: bam
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path '*.version.txt' , emit: version
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path '*.version.txt' , emit: version
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script:
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script:
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def software = getSoftwareName(task.process)
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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"""
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"""
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gatk SplitNCigarReads \\
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gatk SplitNCigarReads \\
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-R $fasta \\
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-R $fasta \\
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-I $bam \\
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-I $bam \\
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-O ${prefix}.split_cigar.bam \\
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-O ${prefix}.bam \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
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echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
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"""
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"""
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}
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}
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@ -2,7 +2,7 @@
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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|
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params.options = [:]
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params.options = [:]
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def options = initOptions(params.options)
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options = initOptions(params.options)
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process GATK4_VARIANTFILTRATION {
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process GATK4_VARIANTFILTRATION {
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tag "$meta.id"
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tag "$meta.id"
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@ -20,9 +20,9 @@ process GATK4_VARIANTFILTRATION {
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input:
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input:
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tuple val(meta), path(vcf)
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tuple val(meta), path(vcf)
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path fasta
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path fasta
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path fai
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path fai
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path dict
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path dict
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output:
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output:
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tuple val(meta), path("*.vcf"), emit: vcf
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tuple val(meta), path("*.vcf"), emit: vcf
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@ -39,6 +39,6 @@ process GATK4_VARIANTFILTRATION {
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-O ${prefix}.vcf \\
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-O ${prefix}.vcf \\
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$options.args
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$options.args
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gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
|
echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt
|
||||||
"""
|
"""
|
||||||
}
|
}
|
||||||
|
|
|
@ -2,9 +2,9 @@
|
||||||
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
||||||
|
|
||||||
params.options = [:]
|
params.options = [:]
|
||||||
def options = initOptions(params.options)
|
options = initOptions(params.options)
|
||||||
|
|
||||||
process KRAKEN2_RUN {
|
process KRAKEN2_KRAKEN2 {
|
||||||
tag "$meta.id"
|
tag "$meta.id"
|
||||||
label 'process_high'
|
label 'process_high'
|
||||||
publishDir "${params.outdir}",
|
publishDir "${params.outdir}",
|
59
software/kraken2/kraken2/meta.yml
Normal file
59
software/kraken2/kraken2/meta.yml
Normal file
|
@ -0,0 +1,59 @@
|
||||||
|
name: kraken2_kraken2
|
||||||
|
description: Classifies metagenomic sequence data
|
||||||
|
keywords:
|
||||||
|
- classify
|
||||||
|
- metagenomics
|
||||||
|
- fastq
|
||||||
|
- db
|
||||||
|
tools:
|
||||||
|
- kraken2:
|
||||||
|
description: |
|
||||||
|
Kraken2 is a taxonomic sequence classifier that assigns taxonomic labels to sequence reads
|
||||||
|
homepage: https://ccb.jhu.edu/software/kraken2/
|
||||||
|
documentation: https://github.com/DerrickWood/kraken2/wiki/Manual
|
||||||
|
doi: 10.1186/s13059-019-1891-0
|
||||||
|
input:
|
||||||
|
- meta:
|
||||||
|
type: map
|
||||||
|
description: |
|
||||||
|
Groovy Map containing sample information
|
||||||
|
e.g. [ id:'test', single_end:false ]
|
||||||
|
- reads:
|
||||||
|
type: file
|
||||||
|
description: |
|
||||||
|
List of input FastQ files of size 1 and 2 for single-end and paired-end data,
|
||||||
|
respectively.
|
||||||
|
- db:
|
||||||
|
type: directory
|
||||||
|
description: Kraken2 database
|
||||||
|
output:
|
||||||
|
- meta:
|
||||||
|
type: map
|
||||||
|
description: |
|
||||||
|
Groovy Map containing sample information
|
||||||
|
e.g. [ id:'test', single_end:false ]
|
||||||
|
- classified:
|
||||||
|
type: file
|
||||||
|
description: |
|
||||||
|
Reads classified to belong to any of the taxa
|
||||||
|
on the Kraken2 database.
|
||||||
|
pattern: "*{fastq.gz}"
|
||||||
|
- unclassified:
|
||||||
|
type: file
|
||||||
|
description: |
|
||||||
|
Reads not classified to belong to any of the taxa
|
||||||
|
on the Kraken2 database.
|
||||||
|
pattern: "*{fastq.gz}"
|
||||||
|
- txt:
|
||||||
|
type: file
|
||||||
|
description: |
|
||||||
|
Kraken2 report containing stats about classified
|
||||||
|
and not classifed reads.
|
||||||
|
pattern: "*.{report.txt}"
|
||||||
|
- version:
|
||||||
|
type: file
|
||||||
|
description: File containing software version
|
||||||
|
pattern: "*.{version.txt}"
|
||||||
|
authors:
|
||||||
|
- "@joseespinosa"
|
||||||
|
- "@drpatelh"
|
|
@ -1,59 +0,0 @@
|
||||||
name: kraken2_run
|
|
||||||
description: Classifies metagenomic sequence data
|
|
||||||
keywords:
|
|
||||||
- classify
|
|
||||||
- metagenomics
|
|
||||||
- fastq
|
|
||||||
- db
|
|
||||||
tools:
|
|
||||||
- kraken2:
|
|
||||||
description: |
|
|
||||||
Kraken2 is a taxonomic sequence classifier that assigns taxonomic labels to sequence reads
|
|
||||||
homepage: https://ccb.jhu.edu/software/kraken2/
|
|
||||||
documentation: https://github.com/DerrickWood/kraken2/wiki/Manual
|
|
||||||
doi: 10.1186/s13059-019-1891-0
|
|
||||||
input:
|
|
||||||
- meta:
|
|
||||||
type: map
|
|
||||||
description: |
|
|
||||||
Groovy Map containing sample information
|
|
||||||
e.g. [ id:'test', single_end:false ]
|
|
||||||
- reads:
|
|
||||||
type: file
|
|
||||||
description: |
|
|
||||||
List of input FastQ files of size 1 and 2 for single-end and paired-end data,
|
|
||||||
respectively.
|
|
||||||
- db:
|
|
||||||
type: directory
|
|
||||||
description: Kraken2 database
|
|
||||||
output:
|
|
||||||
- meta:
|
|
||||||
type: map
|
|
||||||
description: |
|
|
||||||
Groovy Map containing sample information
|
|
||||||
e.g. [ id:'test', single_end:false ]
|
|
||||||
- classified:
|
|
||||||
type: file
|
|
||||||
description: |
|
|
||||||
Reads classified to belong to any of the taxa
|
|
||||||
on the Kraken2 database.
|
|
||||||
pattern: "*{fastq.gz}"
|
|
||||||
- unclassified:
|
|
||||||
type: file
|
|
||||||
description: |
|
|
||||||
Reads not classified to belong to any of the taxa
|
|
||||||
on the Kraken2 database.
|
|
||||||
pattern: "*{fastq.gz}"
|
|
||||||
- txt:
|
|
||||||
type: file
|
|
||||||
description: |
|
|
||||||
Kraken2 report containing stats about classified
|
|
||||||
and not classifed reads.
|
|
||||||
pattern: "*.{report.txt}"
|
|
||||||
- version:
|
|
||||||
type: file
|
|
||||||
description: File containing software version
|
|
||||||
pattern: "*.{version.txt}"
|
|
||||||
authors:
|
|
||||||
- "@joseespinosa"
|
|
||||||
- "@drpatelh"
|
|
|
@ -2,7 +2,8 @@
|
||||||
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
||||||
|
|
||||||
params.options = [:]
|
params.options = [:]
|
||||||
def options = initOptions(params.options)
|
options = initOptions(params.options)
|
||||||
|
|
||||||
process MINIA {
|
process MINIA {
|
||||||
tag "$meta.id"
|
tag "$meta.id"
|
||||||
label 'process_high'
|
label 'process_high'
|
||||||
|
@ -36,6 +37,7 @@ process MINIA {
|
||||||
-nb-cores $task.cpus \\
|
-nb-cores $task.cpus \\
|
||||||
-in input_files.txt \\
|
-in input_files.txt \\
|
||||||
-out $prefix
|
-out $prefix
|
||||||
|
|
||||||
echo \$(minia --version 2>&1) | sed 's/^.*Minia version //; s/ .*\$//' > ${software}.version.txt
|
echo \$(minia --version 2>&1) | sed 's/^.*Minia version //; s/ .*\$//' > ${software}.version.txt
|
||||||
"""
|
"""
|
||||||
}
|
}
|
||||||
|
|
|
@ -2,7 +2,7 @@
|
||||||
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
||||||
|
|
||||||
params.options = [:]
|
params.options = [:]
|
||||||
def options = initOptions(params.options)
|
options = initOptions(params.options)
|
||||||
|
|
||||||
process NANOLYSE {
|
process NANOLYSE {
|
||||||
tag "$meta.id"
|
tag "$meta.id"
|
||||||
|
@ -20,7 +20,7 @@ process NANOLYSE {
|
||||||
|
|
||||||
input:
|
input:
|
||||||
tuple val(meta), path(fastq)
|
tuple val(meta), path(fastq)
|
||||||
path fasta
|
path fasta
|
||||||
|
|
||||||
output:
|
output:
|
||||||
tuple val(meta), path("*.fastq.gz"), emit: fastq
|
tuple val(meta), path("*.fastq.gz"), emit: fastq
|
||||||
|
@ -29,7 +29,7 @@ process NANOLYSE {
|
||||||
|
|
||||||
script:
|
script:
|
||||||
def software = getSoftwareName(task.process)
|
def software = getSoftwareName(task.process)
|
||||||
def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
||||||
"""
|
"""
|
||||||
gunzip -c $fastq | NanoLyse -r $fasta | gzip > ${prefix}.fastq.gz
|
gunzip -c $fastq | NanoLyse -r $fasta | gzip > ${prefix}.fastq.gz
|
||||||
mv NanoLyse.log ${prefix}.nanolyse.log
|
mv NanoLyse.log ${prefix}.nanolyse.log
|
||||||
|
|
|
@ -27,7 +27,7 @@ process PANGOLIN {
|
||||||
|
|
||||||
script:
|
script:
|
||||||
def software = getSoftwareName(task.process)
|
def software = getSoftwareName(task.process)
|
||||||
def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
||||||
"""
|
"""
|
||||||
pangolin \\
|
pangolin \\
|
||||||
$fasta\\
|
$fasta\\
|
||||||
|
@ -35,6 +35,6 @@ process PANGOLIN {
|
||||||
--threads $task.cpus \\
|
--threads $task.cpus \\
|
||||||
$options.args
|
$options.args
|
||||||
|
|
||||||
pangolin --version | sed "s/pangolin //g" > ${software}.version.txt
|
echo \$(pangolin --version) | sed "s/pangolin //g" > ${software}.version.txt
|
||||||
"""
|
"""
|
||||||
}
|
}
|
||||||
|
|
|
@ -25,30 +25,29 @@ process STRELKA_GERMLINE {
|
||||||
path target_bed
|
path target_bed
|
||||||
|
|
||||||
output:
|
output:
|
||||||
tuple val(meta), path("*_variants.vcf.gz") , emit: vcf
|
tuple val(meta), path("*variants.vcf.gz") , emit: vcf
|
||||||
tuple val(meta), path("*_variants.vcf.gz.tbi"), emit: vcf_tbi
|
tuple val(meta), path("*variants.vcf.gz.tbi"), emit: vcf_tbi
|
||||||
tuple val(meta), path("*_genome.vcf.gz") , emit: genome_vcf
|
tuple val(meta), path("*genome.vcf.gz") , emit: genome_vcf
|
||||||
tuple val(meta), path("*_genome.vcf.gz.tbi") , emit: genome_vcf_tbi
|
tuple val(meta), path("*genome.vcf.gz.tbi") , emit: genome_vcf_tbi
|
||||||
path "*.version.txt" , emit: version
|
path "*.version.txt" , emit: version
|
||||||
|
|
||||||
script:
|
script:
|
||||||
def software = getSoftwareName(task.process)
|
def software = getSoftwareName(task.process)
|
||||||
def ioptions = initOptions(options)
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
||||||
def prefix = ioptions.suffix ? "strelka_${meta.id}${ioptions.suffix}" : "strelka_${meta.id}"
|
def regions = params.target_bed ? "--exome --callRegions ${target_bed}" : ""
|
||||||
def options_strelka = params.target_bed ? "--exome --callRegions ${target_bed}" : ""
|
|
||||||
"""
|
"""
|
||||||
configureStrelkaGermlineWorkflow.py \\
|
configureStrelkaGermlineWorkflow.py \\
|
||||||
--bam $bam \\
|
--bam $bam \\
|
||||||
--referenceFasta $fasta \\
|
--referenceFasta $fasta \\
|
||||||
$options_strelka \\
|
$regions \\
|
||||||
$options.args \\
|
$options.args \\
|
||||||
--runDir strelka
|
--runDir strelka
|
||||||
|
|
||||||
python strelka/runWorkflow.py -m local -j $task.cpus
|
python strelka/runWorkflow.py -m local -j $task.cpus
|
||||||
mv strelka/results/variants/genome.*.vcf.gz ${prefix}_genome.vcf.gz
|
mv strelka/results/variants/genome.*.vcf.gz ${prefix}.genome.vcf.gz
|
||||||
mv strelka/results/variants/genome.*.vcf.gz.tbi ${prefix}_genome.vcf.gz.tbi
|
mv strelka/results/variants/genome.*.vcf.gz.tbi ${prefix}.genome.vcf.gz.tbi
|
||||||
mv strelka/results/variants/variants.vcf.gz ${prefix}_variants.vcf.gz
|
mv strelka/results/variants/variants.vcf.gz ${prefix}.variants.vcf.gz
|
||||||
mv strelka/results/variants/variants.vcf.gz.tbi ${prefix}_variants.vcf.gz.tbi
|
mv strelka/results/variants/variants.vcf.gz.tbi ${prefix}.variants.vcf.gz.tbi
|
||||||
|
|
||||||
echo configureStrelkaGermlineWorkflow.py --version &> ${software}.version.txt #2>&1
|
echo configureStrelkaGermlineWorkflow.py --version &> ${software}.version.txt #2>&1
|
||||||
"""
|
"""
|
||||||
|
|
|
@ -2,13 +2,13 @@
|
||||||
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
||||||
|
|
||||||
params.options = [:]
|
params.options = [:]
|
||||||
def options = initOptions(params.options)
|
options = initOptions(params.options)
|
||||||
|
|
||||||
process STRINGTIE_MERGE {
|
process STRINGTIE_MERGE {
|
||||||
label 'process_medium'
|
label 'process_medium'
|
||||||
publishDir "${params.outdir}",
|
publishDir "${params.outdir}",
|
||||||
mode: params.publish_dir_mode,
|
mode: params.publish_dir_mode,
|
||||||
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['']) }
|
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) }
|
||||||
|
|
||||||
// Note: 2.7X indices incompatible with AWS iGenomes.
|
// Note: 2.7X indices incompatible with AWS iGenomes.
|
||||||
conda (params.enable_conda ? "bioconda::stringtie=2.1.4" : null)
|
conda (params.enable_conda ? "bioconda::stringtie=2.1.4" : null)
|
||||||
|
@ -19,11 +19,11 @@ process STRINGTIE_MERGE {
|
||||||
}
|
}
|
||||||
|
|
||||||
input:
|
input:
|
||||||
path stringtie_gtf
|
path stringtie_gtf
|
||||||
path annotation_gtf
|
path annotation_gtf
|
||||||
|
|
||||||
output:
|
output:
|
||||||
path "stringtie.merged.gtf" , emit: merged_gtf
|
path "stringtie.merged.gtf", emit: gtf
|
||||||
|
|
||||||
script:
|
script:
|
||||||
"""
|
"""
|
||||||
|
|
|
@ -20,25 +20,25 @@ process TIDDIT_SV {
|
||||||
|
|
||||||
input:
|
input:
|
||||||
tuple val(meta), path(bam)
|
tuple val(meta), path(bam)
|
||||||
path fasta
|
path fasta
|
||||||
path fai
|
path fai
|
||||||
|
|
||||||
output:
|
output:
|
||||||
tuple val(meta), path("*.vcf"), emit: vcf
|
tuple val(meta), path("*.vcf") , emit: vcf
|
||||||
tuple val(meta), path("*.ploidy.tab"), emit: ploidy
|
tuple val(meta), path("*.ploidy.tab") , emit: ploidy
|
||||||
tuple val(meta), path("*.signals.tab"), emit: signals
|
tuple val(meta), path("*.signals.tab"), emit: signals
|
||||||
path "*.version.txt", emit: version
|
path "*.version.txt" , emit: version
|
||||||
|
|
||||||
script:
|
script:
|
||||||
def software = getSoftwareName(task.process)
|
def software = getSoftwareName(task.process)
|
||||||
def output = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
||||||
def reference = fasta == "dummy_file.txt" ? "--ref $fasta" : ""
|
def reference = fasta == "dummy_file.txt" ? "--ref $fasta" : ""
|
||||||
"""
|
"""
|
||||||
tiddit \\
|
tiddit \\
|
||||||
--sv $options.args \\
|
--sv $options.args \\
|
||||||
--bam $bam \\
|
--bam $bam \\
|
||||||
$reference \\
|
$reference \\
|
||||||
-o $output
|
-o $prefix
|
||||||
|
|
||||||
echo \$(tiddit -h 2>&1) | sed 's/^.*Version: //; s/(.*\$//' > ${software}.version.txt
|
echo \$(tiddit -h 2>&1) | sed 's/^.*Version: //; s/(.*\$//' > ${software}.version.txt
|
||||||
"""
|
"""
|
||||||
|
|
|
@ -2,7 +2,7 @@
|
||||||
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
||||||
|
|
||||||
params.options = [:]
|
params.options = [:]
|
||||||
def options = initOptions(params.options)
|
options = initOptions(params.options)
|
||||||
|
|
||||||
def VERSION = '377'
|
def VERSION = '377'
|
||||||
|
|
||||||
|
@ -36,6 +36,7 @@ process UCSC_BED12TOBIGBED {
|
||||||
$bed \\
|
$bed \\
|
||||||
$sizes \\
|
$sizes \\
|
||||||
${prefix}.bigBed
|
${prefix}.bigBed
|
||||||
|
|
||||||
echo $VERSION > ${software}.version.txt
|
echo $VERSION > ${software}.version.txt
|
||||||
"""
|
"""
|
||||||
}
|
}
|
||||||
|
|
46
software/ucsc/bed12tobigbed/meta.yml
Executable file
46
software/ucsc/bed12tobigbed/meta.yml
Executable file
|
@ -0,0 +1,46 @@
|
||||||
|
name: ucsc_bed12tobigbed
|
||||||
|
description: Convert file from bed12 to bigBed format
|
||||||
|
keywords:
|
||||||
|
- bed12
|
||||||
|
- bigbed
|
||||||
|
tools:
|
||||||
|
- ucsc:
|
||||||
|
description: Convert file from bed12 to bigBed format
|
||||||
|
homepage: None
|
||||||
|
documentation: None
|
||||||
|
tool_dev_url: None
|
||||||
|
doi: ""
|
||||||
|
licence: ["varies; see http://genome.ucsc.edu/license"]
|
||||||
|
|
||||||
|
input:
|
||||||
|
- meta:
|
||||||
|
type: map
|
||||||
|
description: |
|
||||||
|
Groovy Map containing sample information
|
||||||
|
e.g. [ id:'test', single_end:false ]
|
||||||
|
- bed:
|
||||||
|
type: file
|
||||||
|
description: bed12 file
|
||||||
|
pattern: "*.{bed,bed12}"
|
||||||
|
- sizes:
|
||||||
|
type: file
|
||||||
|
description: chromosome sizes file
|
||||||
|
pattern: "*.{sizes}"
|
||||||
|
|
||||||
|
output:
|
||||||
|
- meta:
|
||||||
|
type: map
|
||||||
|
description: |
|
||||||
|
Groovy Map containing sample information
|
||||||
|
e.g. [ id:'test', single_end:false ]
|
||||||
|
- version:
|
||||||
|
type: file
|
||||||
|
description: File containing software version
|
||||||
|
pattern: "*.{version.txt}"
|
||||||
|
- bigbed:
|
||||||
|
type: file
|
||||||
|
description: bigBed file
|
||||||
|
pattern: "*.{bigBed}"
|
||||||
|
|
||||||
|
authors:
|
||||||
|
- "@drpatelh"
|
46
software/ucsc/bedgraphtobigwig/meta.yml
Executable file
46
software/ucsc/bedgraphtobigwig/meta.yml
Executable file
|
@ -0,0 +1,46 @@
|
||||||
|
name: ucsc_bedgraphtobigwig
|
||||||
|
description: Convert a bedGraph file to bigWig format.
|
||||||
|
keywords:
|
||||||
|
- bedgraph
|
||||||
|
- bigwig
|
||||||
|
tools:
|
||||||
|
- ucsc:
|
||||||
|
description: Convert a bedGraph file to bigWig format.
|
||||||
|
homepage: None
|
||||||
|
documentation: None
|
||||||
|
tool_dev_url: None
|
||||||
|
doi: ""
|
||||||
|
licence: ["varies; see http://genome.ucsc.edu/license"]
|
||||||
|
|
||||||
|
input:
|
||||||
|
- meta:
|
||||||
|
type: map
|
||||||
|
description: |
|
||||||
|
Groovy Map containing sample information
|
||||||
|
e.g. [ id:'test', single_end:false ]
|
||||||
|
- bedgraph:
|
||||||
|
type: file
|
||||||
|
description: bedGraph file
|
||||||
|
pattern: "*.{bedGraph}"
|
||||||
|
- sizes:
|
||||||
|
type: file
|
||||||
|
description: chromosome sizes file
|
||||||
|
pattern: "*.{sizes}"
|
||||||
|
|
||||||
|
output:
|
||||||
|
- meta:
|
||||||
|
type: map
|
||||||
|
description: |
|
||||||
|
Groovy Map containing sample information
|
||||||
|
e.g. [ id:'test', single_end:false ]
|
||||||
|
- version:
|
||||||
|
type: file
|
||||||
|
description: File containing software version
|
||||||
|
pattern: "*.{version.txt}"
|
||||||
|
- bigwig:
|
||||||
|
type: file
|
||||||
|
description: bigWig file
|
||||||
|
pattern: "*.{bigWig}"
|
||||||
|
|
||||||
|
authors:
|
||||||
|
- "@drpatelh"
|
|
@ -385,10 +385,10 @@ kallistobustools/ref:
|
||||||
- software/kallistobustools/ref/**
|
- software/kallistobustools/ref/**
|
||||||
- tests/software/kallistobustools/ref/**
|
- tests/software/kallistobustools/ref/**
|
||||||
|
|
||||||
kraken2/run:
|
kraken2/kraken2:
|
||||||
- software/kraken2/run/**
|
- software/kraken2/kraken2/**
|
||||||
- software/untar/**
|
- software/untar/**
|
||||||
- tests/software/kraken2/run/**
|
- tests/software/kraken2/kraken2/**
|
||||||
|
|
||||||
last/dotplot:
|
last/dotplot:
|
||||||
- software/last/dotplot/**
|
- software/last/dotplot/**
|
||||||
|
|
|
@ -9,19 +9,19 @@
|
||||||
md5sum: d41d8cd98f00b204e9800998ecf8427e
|
md5sum: d41d8cd98f00b204e9800998ecf8427e
|
||||||
- path: output/cnvkit/reference.cnn
|
- path: output/cnvkit/reference.cnn
|
||||||
md5sum: ac99c1ad8b917b96ae15119146c91ab9
|
md5sum: ac99c1ad8b917b96ae15119146c91ab9
|
||||||
- path: output/cnvkit/test_paired_end.sorted.targetcoverage.cnn
|
- path: output/cnvkit/test.paired_end.sorted.targetcoverage.cnn
|
||||||
md5sum: 3fe80b6013ffc3e9968345e810158215
|
md5sum: 3fe80b6013ffc3e9968345e810158215
|
||||||
- path: output/cnvkit/test_paired_end.sorted.antitargetcoverage.cnn
|
- path: output/cnvkit/test.paired_end.sorted.antitargetcoverage.cnn
|
||||||
md5sum: 203caf8cef6935bb50b4138097955cb8
|
md5sum: 203caf8cef6935bb50b4138097955cb8
|
||||||
- path: output/cnvkit/test_single_end.sorted.targetcoverage.cnn
|
- path: output/cnvkit/test.single_end.sorted.targetcoverage.cnn
|
||||||
md5sum: aa8a018b1d4d1e688c9f9f6ae01bf4d7
|
md5sum: aa8a018b1d4d1e688c9f9f6ae01bf4d7
|
||||||
- path: output/cnvkit/test_single_end.sorted.antitargetcoverage.cnn
|
- path: output/cnvkit/test.single_end.sorted.antitargetcoverage.cnn
|
||||||
md5sum: 203caf8cef6935bb50b4138097955cb8
|
md5sum: 203caf8cef6935bb50b4138097955cb8
|
||||||
- path: output/cnvkit/test_paired_end.sorted.cnr
|
- path: output/cnvkit/test.paired_end.sorted.cnr
|
||||||
md5sum: 7e37d73ab604dbc3fe4ebb56aca9bdc3
|
md5sum: 7e37d73ab604dbc3fe4ebb56aca9bdc3
|
||||||
- path: output/cnvkit/test_paired_end.sorted.cns
|
- path: output/cnvkit/test.paired_end.sorted.cns
|
||||||
md5sum: 060af1aa637ed51812af19bcce24fcfe
|
md5sum: 060af1aa637ed51812af19bcce24fcfe
|
||||||
- path: output/cnvkit/test_paired_end.sorted.bintest.cns
|
- path: output/cnvkit/test.paired_end.sorted.bintest.cns
|
||||||
md5sum: 6544d979475def8a9f69ba42a985668d
|
md5sum: 6544d979475def8a9f69ba42a985668d
|
||||||
- path: output/cnvkit/test_paired_end.sorted.call.cns
|
- path: output/cnvkit/test.paired_end.sorted.call.cns
|
||||||
md5sum: f2ca59b4d50b0c317adc526c1b99b622
|
md5sum: f2ca59b4d50b0c317adc526c1b99b622
|
||||||
|
|
|
@ -5,7 +5,7 @@
|
||||||
- gatk4/applybqsr
|
- gatk4/applybqsr
|
||||||
files:
|
files:
|
||||||
- path: output/gatk4/test.bam
|
- path: output/gatk4/test.bam
|
||||||
md5sum: 139a5ffc7601ce3cfa7a896cd381542a
|
md5sum: dac716c394db5e83c12b44355c098ca7
|
||||||
|
|
||||||
- name: gatk4 applybqsr test_gatk4_applybqsr_intervals
|
- name: gatk4 applybqsr test_gatk4_applybqsr_intervals
|
||||||
command: nextflow run tests/software/gatk4/applybqsr -entry test_gatk4_applybqsr_intervals -c tests/config/nextflow.config
|
command: nextflow run tests/software/gatk4/applybqsr -entry test_gatk4_applybqsr_intervals -c tests/config/nextflow.config
|
||||||
|
@ -14,4 +14,4 @@
|
||||||
- gatk4/applybqsr
|
- gatk4/applybqsr
|
||||||
files:
|
files:
|
||||||
- path: output/gatk4/test.bam
|
- path: output/gatk4/test.bam
|
||||||
md5sum: 5b1f6fa2525124c281f71e5a76d28482
|
md5sum: 400441dbe5344658580ba0a24ba57069
|
||||||
|
|
|
@ -5,4 +5,4 @@
|
||||||
- gatk4/markduplicates
|
- gatk4/markduplicates
|
||||||
files:
|
files:
|
||||||
- path: output/gatk4/test.bam
|
- path: output/gatk4/test.bam
|
||||||
md5sum: b58d47345e3ce5825c0641c9d6e6cb7a
|
md5sum: 3b6facab3afbacfa08a7a975efbd2c6b
|
||||||
|
|
|
@ -4,5 +4,5 @@
|
||||||
- gatk4
|
- gatk4
|
||||||
- gatk4/mergebamalignment
|
- gatk4/mergebamalignment
|
||||||
files:
|
files:
|
||||||
- path: output/gatk4/test.merged.bam
|
- path: output/gatk4/test.bam
|
||||||
md5sum: bd4a5e2ea916826aadebb5878333e26f
|
md5sum: bd4a5e2ea916826aadebb5878333e26f
|
||||||
|
|
|
@ -4,7 +4,7 @@
|
||||||
- gatk4/mergevcfs
|
- gatk4/mergevcfs
|
||||||
- gatk4
|
- gatk4
|
||||||
files:
|
files:
|
||||||
- path: output/gatk4/test.merged.vcf.gz
|
- path: output/gatk4/test.vcf.gz
|
||||||
md5sum: ff48f175e26db2d4b2957762f6d1c715
|
md5sum: ff48f175e26db2d4b2957762f6d1c715
|
||||||
|
|
||||||
- name: gatk4 mergevcfs test_gatk4_mergevcfs_refdict
|
- name: gatk4 mergevcfs test_gatk4_mergevcfs_refdict
|
||||||
|
@ -13,5 +13,5 @@
|
||||||
- gatk4/mergevcfs
|
- gatk4/mergevcfs
|
||||||
- gatk4
|
- gatk4
|
||||||
files:
|
files:
|
||||||
- path: output/gatk4/test.merged.vcf.gz
|
- path: output/gatk4/test.vcf.gz
|
||||||
md5sum: ff48f175e26db2d4b2957762f6d1c715
|
md5sum: ff48f175e26db2d4b2957762f6d1c715
|
||||||
|
|
|
@ -4,5 +4,5 @@
|
||||||
- gatk4
|
- gatk4
|
||||||
- gatk4/splitncigarreads
|
- gatk4/splitncigarreads
|
||||||
files:
|
files:
|
||||||
- path: output/gatk4/test.split_cigar.bam
|
- path: output/gatk4/test.bam
|
||||||
md5sum: a8473bd4abc7b13751fc51b92531da4c
|
md5sum: 900af0f67749d5ffac961354178ecb29
|
||||||
|
|
|
@ -2,20 +2,20 @@
|
||||||
|
|
||||||
nextflow.enable.dsl = 2
|
nextflow.enable.dsl = 2
|
||||||
|
|
||||||
include { UNTAR } from '../../../../software/untar/main.nf' addParams( options: [:] )
|
include { UNTAR } from '../../../../software/untar/main.nf' addParams( options: [:] )
|
||||||
include { KRAKEN2_RUN } from '../../../../software/kraken2/run/main.nf' addParams( options: [:] )
|
include { KRAKEN2_KRAKEN2 } from '../../../../software/kraken2/kraken2/main.nf' addParams( options: [:] )
|
||||||
|
|
||||||
workflow test_kraken2_run_single_end {
|
workflow test_kraken2_kraken2_single_end {
|
||||||
input = [ [ id:'test', single_end:true ], // meta map
|
input = [ [ id:'test', single_end:true ], // meta map
|
||||||
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
|
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
|
||||||
]
|
]
|
||||||
db = file(params.test_data['sarscov2']['genome']['kraken2_tar_gz'], checkIfExists: true)
|
db = file(params.test_data['sarscov2']['genome']['kraken2_tar_gz'], checkIfExists: true)
|
||||||
|
|
||||||
UNTAR ( db )
|
UNTAR ( db )
|
||||||
KRAKEN2_RUN ( input, UNTAR.out.untar )
|
KRAKEN2_KRAKEN2 ( input, UNTAR.out.untar )
|
||||||
}
|
}
|
||||||
|
|
||||||
workflow test_kraken2_run_paired_end {
|
workflow test_kraken2_kraken2_paired_end {
|
||||||
input = [ [ id:'test', single_end:false ], // meta map
|
input = [ [ id:'test', single_end:false ], // meta map
|
||||||
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
|
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
|
||||||
file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
|
file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
|
||||||
|
@ -23,5 +23,5 @@ workflow test_kraken2_run_paired_end {
|
||||||
db = file(params.test_data['sarscov2']['genome']['kraken2_tar_gz'], checkIfExists: true)
|
db = file(params.test_data['sarscov2']['genome']['kraken2_tar_gz'], checkIfExists: true)
|
||||||
|
|
||||||
UNTAR ( db )
|
UNTAR ( db )
|
||||||
KRAKEN2_RUN ( input, UNTAR.out.untar )
|
KRAKEN2_KRAKEN2 ( input, UNTAR.out.untar )
|
||||||
}
|
}
|
|
@ -1,8 +1,8 @@
|
||||||
- name: kraken2 run single-end
|
- name: kraken2 kraken2 single-end
|
||||||
command: nextflow run ./tests/software/kraken2/run -entry test_kraken2_run_single_end -c tests/config/nextflow.config
|
command: nextflow run ./tests/software/kraken2/kraken2 -entry test_kraken2_kraken2_single_end -c tests/config/nextflow.config
|
||||||
tags:
|
tags:
|
||||||
- kraken2
|
- kraken2
|
||||||
- kraken2/run
|
- kraken2/kraken2
|
||||||
files:
|
files:
|
||||||
- path: output/kraken2/test.classified.fastq.gz
|
- path: output/kraken2/test.classified.fastq.gz
|
||||||
should_exist: true
|
should_exist: true
|
||||||
|
@ -11,11 +11,11 @@
|
||||||
- path: output/kraken2/test.kraken2.report.txt
|
- path: output/kraken2/test.kraken2.report.txt
|
||||||
md5sum: 4227755fe40478b8d7dc8634b489761e
|
md5sum: 4227755fe40478b8d7dc8634b489761e
|
||||||
|
|
||||||
- name: kraken2 run paired-end
|
- name: kraken2 kraken2 paired-end
|
||||||
command: nextflow run ./tests/software/kraken2/run -entry test_kraken2_run_paired_end -c tests/config/nextflow.config
|
command: nextflow run ./tests/software/kraken2/kraken2 -entry test_kraken2_kraken2_paired_end -c tests/config/nextflow.config
|
||||||
tags:
|
tags:
|
||||||
- kraken2
|
- kraken2
|
||||||
- kraken2/run
|
- kraken2/kraken2
|
||||||
files:
|
files:
|
||||||
- path: output/kraken2/test.classified_1.fastq.gz
|
- path: output/kraken2/test.classified_1.fastq.gz
|
||||||
should_exist: true
|
should_exist: true
|
|
@ -2,23 +2,15 @@
|
||||||
|
|
||||||
nextflow.enable.dsl = 2
|
nextflow.enable.dsl = 2
|
||||||
|
|
||||||
include { NANOLYSE } from '../../../software/nanolyse/main.nf' addParams( options: [suffix: 'clean'] )
|
include { NANOLYSE } from '../../../software/nanolyse/main.nf' addParams( options: [suffix: '.clean'] )
|
||||||
|
|
||||||
process GET_NANOLYSE_FASTA {
|
|
||||||
output:
|
|
||||||
path "*fasta.gz", emit: fasta
|
|
||||||
|
|
||||||
script:
|
|
||||||
"""
|
|
||||||
wget https://github.com/wdecoster/nanolyse/raw/master/reference/lambda.fasta.gz
|
|
||||||
"""
|
|
||||||
}
|
|
||||||
|
|
||||||
workflow test_nanolyse {
|
workflow test_nanolyse {
|
||||||
input = [ [ id:'test' ], // meta map
|
input = [
|
||||||
[ file(params.test_data['sarscov2']['nanopore']['test_fastq_gz'], checkIfExists: true)]
|
[ id:'test' ], // meta map
|
||||||
]
|
[ file(params.test_data['sarscov2']['nanopore']['test_fastq_gz'], checkIfExists: true)]
|
||||||
|
]
|
||||||
|
|
||||||
GET_NANOLYSE_FASTA()
|
fasta = file("https://github.com/wdecoster/nanolyse/raw/master/reference/lambda.fasta.gz", checkIfExists: true)
|
||||||
NANOLYSE ( input, GET_NANOLYSE_FASTA.out.fasta )
|
|
||||||
|
NANOLYSE ( input, fasta )
|
||||||
}
|
}
|
||||||
|
|
|
@ -5,25 +5,29 @@ nextflow.enable.dsl = 2
|
||||||
include { STRELKA_GERMLINE } from '../../../../software/strelka/germline/main.nf' addParams( options: [:] )
|
include { STRELKA_GERMLINE } from '../../../../software/strelka/germline/main.nf' addParams( options: [:] )
|
||||||
|
|
||||||
workflow test_strelka_germline {
|
workflow test_strelka_germline {
|
||||||
input = [ [ id:'test'], // meta map
|
input = [
|
||||||
file("${launchDir}/tests/data/genomics/sarscov2/illumina/bam/test_paired_end.sorted.bam", checkIfExists: true),
|
[ id:'test'], // meta map
|
||||||
file("${launchDir}/tests/data/genomics/sarscov2/illumina/bam/test_paired_end.sorted.bam.bai", checkIfExists: true)
|
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
|
||||||
]
|
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
|
||||||
fasta = file("${launchDir}/tests/data/genomics/sarscov2/genome/genome.fasta", checkIfExists: true)
|
]
|
||||||
fai = file("${launchDir}/tests/data/genomics/sarscov2/genome/genome.fasta.fai", checkIfExists: true)
|
|
||||||
|
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||||
|
fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
|
||||||
targets = []
|
targets = []
|
||||||
|
|
||||||
STRELKA_GERMLINE ( input, fasta, fai, targets )
|
STRELKA_GERMLINE ( input, fasta, fai, targets )
|
||||||
}
|
}
|
||||||
|
|
||||||
workflow test_strelka_germline_target_bed {
|
workflow test_strelka_germline_target_bed {
|
||||||
input = [ [ id:'test'], // meta map
|
input = [
|
||||||
file("${launchDir}/tests/data/genomics/sarscov2/illumina/bam/test_paired_end.sorted.bam", checkIfExists: true),
|
[ id:'test'], // meta map
|
||||||
file("${launchDir}/tests/data/genomics/sarscov2/illumina/bam/test_paired_end.sorted.bam.bai", checkIfExists: true)
|
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
|
||||||
]
|
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
|
||||||
fasta = file("${launchDir}/tests/data/genomics/sarscov2/genome/genome.fasta", checkIfExists: true)
|
]
|
||||||
fai = file("${launchDir}/tests/data/genomics/sarscov2/genome/genome.fasta.fai", checkIfExists: true)
|
|
||||||
targets = file("${launchDir}/tests/data/genomics/sarscov2/genome/bed/test.bed", checkIfExists: true)
|
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||||
|
fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
|
||||||
|
targets = file(params.test_data['sarscov2']['genome']['test_bed'], checkIfExists: true)
|
||||||
|
|
||||||
STRELKA_GERMLINE ( input, fasta, fai, targets )
|
STRELKA_GERMLINE ( input, fasta, fai, targets )
|
||||||
}
|
}
|
||||||
|
|
|
@ -1,22 +1,20 @@
|
||||||
## TODO nf-core: Please run the following command to build this file:
|
|
||||||
# nf-core modules create-test-yml strelka/germline
|
|
||||||
- name: strelka germline
|
- name: strelka germline
|
||||||
command: nextflow run ./tests/software/strelka/germline -entry test_strelka_germline -c tests/config/nextflow.config
|
command: nextflow run ./tests/software/strelka/germline -entry test_strelka_germline -c tests/config/nextflow.config
|
||||||
tags:
|
tags:
|
||||||
- strelka
|
- strelka
|
||||||
- strelka/germline
|
- strelka/germline
|
||||||
files:
|
files:
|
||||||
- path: output/strelka/strelka_test_variants.vcf.gz
|
- path: output/strelka/test.variants.vcf.gz
|
||||||
- path: output/strelka/strelka_test_variants.vcf.gz.tbi
|
- path: output/strelka/test.variants.vcf.gz.tbi
|
||||||
- path: output/strelka/strelka_test_genome.vcf.gz
|
- path: output/strelka/test.genome.vcf.gz
|
||||||
- path: output/strelka/strelka_test_genome.vcf.gz.tbi
|
- path: output/strelka/test.genome.vcf.gz.tbi
|
||||||
- name: strelka germline target bed
|
- name: strelka germline target bed
|
||||||
command: nextflow run ./tests/software/strelka/germline -entry test_strelka_germline_target_bed -c tests/config/nextflow.config
|
command: nextflow run ./tests/software/strelka/germline -entry test_strelka_germline_target_bed -c tests/config/nextflow.config
|
||||||
tags:
|
tags:
|
||||||
- strelka
|
- strelka
|
||||||
- strelka/germline
|
- strelka/germline
|
||||||
files:
|
files:
|
||||||
- path: output/strelka/strelka_test_variants.vcf.gz
|
- path: output/strelka/test.variants.vcf.gz
|
||||||
- path: output/strelka/strelka_test_variants.vcf.gz.tbi
|
- path: output/strelka/test.variants.vcf.gz.tbi
|
||||||
- path: output/strelka/strelka_test_genome.vcf.gz
|
- path: output/strelka/test.genome.vcf.gz
|
||||||
- path: output/strelka/strelka_test_genome.vcf.gz.tbi
|
- path: output/strelka/test.genome.vcf.gz.tbi
|
||||||
|
|
|
@ -5,9 +5,11 @@ nextflow.enable.dsl = 2
|
||||||
include { TIDDIT_SV } from '../../../../software/tiddit/sv/main.nf' addParams( options: [:] )
|
include { TIDDIT_SV } from '../../../../software/tiddit/sv/main.nf' addParams( options: [:] )
|
||||||
|
|
||||||
workflow test_tiddit_sv {
|
workflow test_tiddit_sv {
|
||||||
input = [ [ id:'test' ], // meta map
|
input = [
|
||||||
[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
|
[ id:'test' ], // meta map
|
||||||
]
|
[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
|
||||||
|
]
|
||||||
|
|
||||||
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||||
fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
|
fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
|
||||||
|
|
||||||
|
@ -15,9 +17,10 @@ workflow test_tiddit_sv {
|
||||||
}
|
}
|
||||||
|
|
||||||
workflow test_tiddit_sv_no_ref {
|
workflow test_tiddit_sv_no_ref {
|
||||||
input = [ [ id:'test' ], // meta map
|
input = [
|
||||||
[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
|
[ id:'test' ], // meta map
|
||||||
]
|
[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
|
||||||
|
]
|
||||||
|
|
||||||
TIDDIT_SV ( input, [], [] )
|
TIDDIT_SV ( input, [], [] )
|
||||||
}
|
}
|
||||||
|
|
|
@ -7,9 +7,8 @@
|
||||||
- path: output/tiddit/test.ploidy.tab
|
- path: output/tiddit/test.ploidy.tab
|
||||||
md5sum: 45e050b0e204f0a5a3a99627cc440eaa
|
md5sum: 45e050b0e204f0a5a3a99627cc440eaa
|
||||||
- path: output/tiddit/test.signals.tab
|
- path: output/tiddit/test.signals.tab
|
||||||
md5sum: 25408a43dbdd01c08e33a6d670f2bb84
|
md5sum: dab4b2fec4ddf8eb1c23005b0770150e
|
||||||
- path: output/tiddit/test.vcf
|
- path: output/tiddit/test.vcf
|
||||||
should_exist: true
|
|
||||||
|
|
||||||
- name: tiddit sv no ref
|
- name: tiddit sv no ref
|
||||||
command: nextflow run ./tests/software/tiddit/sv -entry test_tiddit_sv_no_ref -c tests/config/nextflow.config
|
command: nextflow run ./tests/software/tiddit/sv -entry test_tiddit_sv_no_ref -c tests/config/nextflow.config
|
||||||
|
@ -20,6 +19,5 @@
|
||||||
- path: output/tiddit/test.ploidy.tab
|
- path: output/tiddit/test.ploidy.tab
|
||||||
md5sum: 45e050b0e204f0a5a3a99627cc440eaa
|
md5sum: 45e050b0e204f0a5a3a99627cc440eaa
|
||||||
- path: output/tiddit/test.signals.tab
|
- path: output/tiddit/test.signals.tab
|
||||||
md5sum: 25408a43dbdd01c08e33a6d670f2bb84
|
md5sum: dab4b2fec4ddf8eb1c23005b0770150e
|
||||||
- path: output/tiddit/test.vcf
|
- path: output/tiddit/test.vcf
|
||||||
should_exist: true
|
|
||||||
|
|
Loading…
Reference in a new issue