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refactor(pytest-workflow): Move fastqc tests to tests/
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10 changed files with 34 additions and 112 deletions
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../../../lib/
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#!/usr/bin/env nextflow
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import checksum
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nextflow.preview.dsl = 2
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params.out_dir = "test_output"
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params.fastqc_args = ''
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params.publish_dir_mode = "copy"
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include { FASTQC } from '../main.nf'
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/**
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* Test if FASTQC runs with single-end data
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*/
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workflow test_single_end {
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input_files = Channel.fromPath("${baseDir}/input/test_single_end.fastq.gz")
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.map {f -> [f.name.replace(".fastq.gz", ""), true, f]}
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FASTQC(input_files)
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// test that the output looks as expected
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FASTQC.out.html.map { name, is_single_end, html_file ->
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html_hash = checksum.getMD5(new File("${html_file}"));
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assert name == "test_single_end"
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assert is_single_end == true
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assert html_file.getName() == "test_single_end_fastqc.html"
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// Hash seems to vary between local runs and GitHub Actions
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// TODO: Might be solved when using Docker for tests?
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// assert html_hash == "8ed68442ebb5b9706bf79b4f66701e15"
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}
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FASTQC.out.zip.map { name, is_single_end, zip_file ->
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// NOTE: output zip files do not have a consistent hash
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assert name == "test_single_end"
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assert is_single_end == true
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assert zip_file.getName() == "test_single_end_fastqc.zip"
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}
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}
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/**
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* Test if FASTQC runs with paired end data
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*/
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workflow test_paired_end {
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input_files = Channel.fromFilePairs("input/test_R{1,2}.fastq.gz")
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.map {f -> [f[0], false, f[1]]}
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FASTQC(input_files)
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// test that the output looks as expected
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FASTQC.out.html.map { name, is_single_end, html_files ->
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html_r1 = html_files[0]
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html_r2 = html_files[1]
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html_r1_hash = checksum.getMD5(new File("${html_r1}"));
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html_r2_hash = checksum.getMD5(new File("${html_r2}"));
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assert name == "test_R"
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assert is_single_end == false
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assert html_r1.getName() == "test_R_1_fastqc.html"
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assert html_r2.getName() == "test_R_2_fastqc.html"
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assert html_r1_hash == "082c13ce7163ea0f52a66b83cb57b0f0"
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assert html_r2_hash == "4ff04ec8da77e3af512f03b8c09a9e04"
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}
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FASTQC.out.zip.map { name, is_single_end, zip_files ->
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zip_r1 = zip_files[0]
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zip_r2 = zip_files[1]
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// NOTE: output zip files do not have a consistent hash
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assert name == "test_R"
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assert is_single_end == false
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assert zip_r1.getName() == "test_R_1_fastqc.zip"
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assert zip_r2.getName() == "test_R_2_fastqc.zip"
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}
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}
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workflow {
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test_single_end()
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test_paired_end()
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}
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../../../../tests/data/fastq/rna/test_single_end.fastq.gz
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#!/usr/bin/env nextflow
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nextflow.enable.dsl = 2
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include { FASTQC as FASTQC_SE } from '../main.nf' addParams( options: [ publish_dir:'test_single_end' ] )
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include { FASTQC as FASTQC_PE } from '../main.nf' addParams( options: [ publish_dir:'test_paired_end' ] )
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/*
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* Test with single-end data
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*/
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workflow test_single_end {
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def input = []
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input = [ [ id:'test', single_end:true ], // meta map
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[ file("${baseDir}/input/test_single_end.fastq.gz", checkIfExists: true) ] ]
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FASTQC_SE ( input )
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}
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/*
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* Test with paired-end data
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*/
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workflow test_paired_end {
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def input = []
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input = [ [ id:'test', single_end:false ], // meta map
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[ file("${baseDir}/input/test_R1.fastq.gz", checkIfExists: true),
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file("${baseDir}/input/test_R2.fastq.gz", checkIfExists: true) ] ]
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FASTQC_PE ( input )
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}
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1
tests/fastqc/input/test_single_end.fastq.gz
Symbolic link
1
tests/fastqc/input/test_single_end.fastq.gz
Symbolic link
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../../data/fastq/rna/test_single_end.fastq.gz
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31
tests/fastqc/main.nf
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31
tests/fastqc/main.nf
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#!/usr/bin/env nextflow
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nextflow.enable.dsl = 2
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include { FASTQC as FASTQC_SE } from '../../software/fastqc/main.nf' addParams( options: [ publish_dir:'test_single_end' ] )
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include { FASTQC as FASTQC_PE } from '../../software/fastqc/main.nf' addParams( options: [ publish_dir:'test_paired_end' ] )
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/*
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* Test with single-end data
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*/
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workflow test_single_end {
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def input = []
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input = [ [ id:'test', single_end:true ], // meta map
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[ file("${projectDir}/input/test_single_end.fastq.gz", checkIfExists: true) ] ]
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FASTQC_SE ( input )
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}
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/*
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* Test with paired-end data
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*/
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workflow test_paired_end {
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def input = []
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input = [ [ id:'test', single_end:false ], // meta map
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[ file("${launchDir}/tests/data/fastq/rna/test_R1.fastq.gz", checkIfExists: true),
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file("${projectDir}/../data/fastq/rna/test_R2.fastq.gz", checkIfExists: true) ] ]
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FASTQC_PE ( input )
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}
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params {
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outdir = "output/"
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publish_dir_mode = "copy"
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- name: Run fastqc single-end test workflow
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command: nextflow run ./software/fastqc/test/ -profile docker -entry test_single_end
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command: nextflow run ./tests/fastqc/ -profile docker -entry test_single_end
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tags:
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- fastqc
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files:
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- path: output/test_single_end/test_fastqc.zip
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- name: Run fastqc paired-end test workflow
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command: nextflow run ./software/fastqc/test/ -profile docker -entry test_paired_end
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command: nextflow run ./tests/fastqc/ -profile docker -entry test_paired_end
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tags:
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- fastqc
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files:
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