diff --git a/modules/antismash/antismashlite/main.nf b/modules/antismash/antismashlite/main.nf
new file mode 100644
index 00000000..fb003a30
--- /dev/null
+++ b/modules/antismash/antismashlite/main.nf
@@ -0,0 +1,68 @@
+process ANTISMASH_ANTISMASHLITE {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda (params.enable_conda ? "bioconda::antismash-lite=6.0.1" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/antismash-lite:6.0.1--pyhdfd78af_1' :
+        'quay.io/biocontainers/antismash-lite:6.0.1--pyhdfd78af_1' }"
+
+    containerOptions {
+        workflow.containerEngine == 'singularity' ?
+        "-B $antismash_dir:/usr/local/lib/python3.8/site-packages/antismash" :
+        workflow.containerEngine == 'docker' ?
+        "-v \$PWD/$antismash_dir:/usr/local/lib/python3.8/site-packages/antismash" :
+        ''
+        }
+
+    input:
+    tuple val(meta), path(sequence_input)
+    path(databases)
+    path(antismash_dir) // Optional input: AntiSMASH installation folder. It is not needed for using this module with conda, but required for docker/singularity (see meta.yml).
+    path(gff)
+
+    output:
+    tuple val(meta), path("${prefix}/clusterblast/*_c*.txt")                 , optional: true, emit: clusterblast_file
+    tuple val(meta), path("${prefix}/{css,images,js}")                       , emit: html_accessory_files
+    tuple val(meta), path("${prefix}/knownclusterblast/region*/ctg*.html")   , optional: true, emit: knownclusterblast_html
+    tuple val(meta), path("${prefix}/knownclusterblast/*_c*.txt")            , optional: true, emit: knownclusterblast_txt
+    tuple val(meta), path("${prefix}/svg/clusterblast*.svg")                 , optional: true, emit: svg_files_clusterblast
+    tuple val(meta), path("${prefix}/svg/knownclusterblast*.svg")            , optional: true, emit: svg_files_knownclusterblast
+    tuple val(meta), path("${prefix}/*.gbk")                                 , emit: gbk_input
+    tuple val(meta), path("${prefix}/*.json")                                , emit: json_results
+    tuple val(meta), path("${prefix}/*.log")                                 , emit: log
+    tuple val(meta), path("${prefix}/*.zip")                                 , emit: zip
+    tuple val(meta), path("${prefix}/*region*.gbk")                          , emit: gbk_results
+    tuple val(meta), path("${prefix}/clusterblastoutput.txt")                , optional: true, emit: clusterblastoutput
+    tuple val(meta), path("${prefix}/index.html")                            , emit: html
+    tuple val(meta), path("${prefix}/knownclusterblastoutput.txt")           , optional: true, emit: knownclusterblastoutput
+    tuple val(meta), path("${prefix}/regions.js")                            , emit: json_sideloading
+    path "versions.yml"                                                      , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    prefix = task.ext.suffix ? "${meta.id}${task.ext.suffix}" : "${meta.id}"
+    gff_flag = "--genefinding-gff3 ${gff}"
+
+    """
+    ## We specifically do not include annotations (--genefinding-tool none) as
+    ## this should be run as a separate module for versioning purposes
+    antismash \\
+        $args \\
+        $gff_flag \\
+        -c $task.cpus \\
+        --output-dir $prefix \\
+        --genefinding-tool none \\
+        --logfile $prefix/${prefix}.log \\
+        --databases $databases \\
+        $sequence_input
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        antismash-lite: \$(antismash --version | sed 's/antiSMASH //')
+    END_VERSIONS
+    """
+}
diff --git a/modules/antismash/antismashlite/meta.yml b/modules/antismash/antismashlite/meta.yml
new file mode 100644
index 00000000..b3e8564e
--- /dev/null
+++ b/modules/antismash/antismashlite/meta.yml
@@ -0,0 +1,128 @@
+name: antismash_antismashlite
+description: |
+  antiSMASH allows the rapid genome-wide identification, annotation
+  and analysis of secondary metabolite biosynthesis gene clusters.
+keywords:
+  - secondary metabolites
+  - BGC
+  - biosynthetic gene cluster
+  - genome mining
+  - NRPS
+  - RiPP
+  - antibiotics
+  - prokaryotes
+  - bacteria
+  - eukaryotes
+  - fungi
+  - antismash
+
+tools:
+  - antismashlite:
+      description: "antiSMASH - the antibiotics and Secondary Metabolite Analysis SHell"
+      homepage: "https://docs.antismash.secondarymetabolites.org"
+      documentation: "https://docs.antismash.secondarymetabolites.org"
+      tool_dev_url: "https://github.com/antismash/antismash"
+      doi: "10.1093/nar/gkab335"
+      licence: "['AGPL v3']"
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - sequence_input:
+      type: file
+      description: nucleotide sequence file (annotated)
+      pattern: "*.{gbk, gb, gbff, genbank, embl, fasta, fna}"
+  - databases:
+      type: directory
+      description: downloaded AntiSMASH databases e.g. data/databases
+      pattern: "*/"
+  - antismash_dir:
+      type: directory
+      description: |
+        A local copy of an AntiSMASH installation folder. This is required when running with
+        docker and singularity (not required for conda), due to attempted 'modifications' of
+        files during database checks in the installation directory, something that cannot
+        be done in immutable docker/singularity containers. Therefore, a local installation
+        directory needs to be mounted (including all modified files from the downloading step)
+        to the container as a workaround.
+      pattern: "*/"
+  - gff:
+      type: file
+      pattern: "*.gff"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - clusterblast_file:
+      type: file
+      description: Output of ClusterBlast algorithm
+      pattern: "clusterblast/*_c*.txt"
+  - html_accessory_files:
+      type: directory
+      description: Accessory files for the HTML output
+      pattern: "{css/,images/,js/}"
+  - knownclusterblast_html:
+      type: file
+      description: Tables with MIBiG hits in HTML format
+      pattern: "knownclusterblast/region*/ctg*.html"
+  - knownclusterblast_txt:
+      type: file
+      description: Tables with MIBiG hits
+      pattern: "knownclusterblast/*_c*.txt"
+  - svg_files_clusterblast:
+      type: file
+      description: SVG images showing the % identity of the aligned hits against their queries
+      pattern: "svg/clusterblast*.svg"
+  - svg_files_knownclusterblast:
+      type: file
+      description: SVG images showing the % identity of the aligned hits against their queries
+      pattern: "svg/knownclusterblast*.svg"
+  - gbk_input:
+      type: file
+      description: Nucleotide sequence and annotations in GenBank format; converted from input file
+      pattern: "*.gbk"
+  - json_results:
+      type: file
+      description: Nucleotide sequence and annotations in JSON format; converted from GenBank file (gbk_input)
+      pattern: "*.json"
+  - log:
+      type: file
+      description: Contains all the logging output that antiSMASH produced during its run
+      pattern: "*.log"
+  - zip:
+      type: file
+      description: Contains a compressed version of the output folder in zip format
+      pattern: "*.zip"
+  - gbk_results:
+      type: file
+      description: Nucleotide sequence and annotations in GenBank format; one file per antiSMASH hit
+      pattern: "*region*.gbk"
+  - clusterblastoutput:
+      type: file
+      description: Raw BLAST output of known clusters previously predicted by antiSMASH using the built-in ClusterBlast algorithm
+      pattern: "clusterblastoutput.txt"
+  - html:
+      type: file
+      description: Graphical web view of results in HTML format
+      patterN: "index.html"
+  - knownclusterblastoutput:
+      type: file
+      description: Raw BLAST output of known clusters of the MIBiG database
+      pattern: "knownclusterblastoutput.txt"
+  - json_sideloading:
+      type: file
+      description: Sideloaded annotations of protoclusters and/or subregions (see antiSMASH documentation "Annotation sideloading")
+      pattern: "regions.js"
+
+authors:
+  - "@jasmezz"
diff --git a/tests/config/pytest_modules.yml b/tests/config/pytest_modules.yml
index bc0aa79a..68c9efef 100644
--- a/tests/config/pytest_modules.yml
+++ b/tests/config/pytest_modules.yml
@@ -42,6 +42,10 @@ amrfinderplus/update:
   - modules/amrfinderplus/update/**
   - tests/modules/amrfinderplus/update/**
 
+antismash/antismashlite:
+  - modules/antismash/antismashlite/**
+  - tests/modules/antismash/antismashlite/**
+
 antismash/antismashlitedownloaddatabases:
   - modules/antismash/antismashlitedownloaddatabases/**
   - tests/modules/antismash/antismashlitedownloaddatabases/**
diff --git a/tests/modules/antismash/antismashlite/main.nf b/tests/modules/antismash/antismashlite/main.nf
new file mode 100644
index 00000000..2b23c400
--- /dev/null
+++ b/tests/modules/antismash/antismashlite/main.nf
@@ -0,0 +1,46 @@
+#!/usr/bin/env nextflow
+
+nextflow.enable.dsl = 2
+
+include { ANTISMASH_ANTISMASHLITE                  } from '../../../../modules/antismash/antismashlite/main.nf'
+include { ANTISMASH_ANTISMASHLITEDOWNLOADDATABASES } from '../../../modules/antismash/antismashlitedownloaddatabases/main.nf'
+include { GUNZIP as GUNZIP1 } from '../../../../modules/gunzip/main.nf'
+include { GUNZIP as GUNZIP2 } from '../../../../modules/gunzip/main.nf'
+include { UNTAR as UNTAR1 } from '../../../../modules/untar/main.nf'
+include { UNTAR as UNTAR2 } from '../../../../modules/untar/main.nf'
+include { UNTAR as UNTAR3 } from '../../../../modules/untar/main.nf'
+
+workflow test_antismashlite {
+    genome_fna = [
+        [ id:'test' ],
+        file(params.test_data['bacteroides_fragilis']['genome']['genome_fna_gz'], checkIfExists: true)
+    ]
+
+    genome_gff = [
+        [],
+        file(params.test_data['bacteroides_fragilis']['genome']['genome_gff_gz'], checkIfExists: true)
+    ]
+
+    antismash_css = [
+        [],
+        file('https://github.com/nf-core/test-datasets/raw/modules/data/delete_me/antismash/css.tar.gz', checkIfExists: true)
+    ]
+
+    antismash_detection = [
+        [],
+        file('https://github.com/nf-core/test-datasets/raw/modules/data/delete_me/antismash/detection.tar.gz', checkIfExists: true)
+    ]
+
+    antismash_modules = [
+        [],
+        file('https://github.com/nf-core/test-datasets/raw/modules/data/delete_me/antismash/modules.tar.gz', checkIfExists: true)
+    ]
+
+    GUNZIP1 ( genome_fna )
+    GUNZIP2 ( genome_gff )
+    UNTAR1 ( antismash_css )
+    UNTAR2 ( antismash_detection )
+    UNTAR3 ( antismash_modules )
+    ANTISMASH_ANTISMASHLITEDOWNLOADDATABASES ( UNTAR1.out.untar.map{ it[1] },  UNTAR2.out.untar.map{ it[1] }, UNTAR3.out.untar.map{ it[1] } )
+    ANTISMASH_ANTISMASHLITE ( GUNZIP1.out.gunzip, ANTISMASH_ANTISMASHLITEDOWNLOADDATABASES.out.database, ANTISMASH_ANTISMASHLITEDOWNLOADDATABASES.out.antismash_dir, GUNZIP2.out.gunzip.map{ it[1] } )
+}
diff --git a/tests/modules/antismash/antismashlite/nextflow.config b/tests/modules/antismash/antismashlite/nextflow.config
new file mode 100644
index 00000000..50f50a7a
--- /dev/null
+++ b/tests/modules/antismash/antismashlite/nextflow.config
@@ -0,0 +1,5 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+    
+}
\ No newline at end of file
diff --git a/tests/modules/antismash/antismashlite/test.yml b/tests/modules/antismash/antismashlite/test.yml
new file mode 100644
index 00000000..4642319c
--- /dev/null
+++ b/tests/modules/antismash/antismashlite/test.yml
@@ -0,0 +1,35 @@
+- name: antismash antismashlite test_antismashlite
+  command: nextflow run tests/modules/antismash/antismashlite -entry test_antismashlite -c tests/config/nextflow.config
+  tags:
+    - antismash/antismashlite
+    - antismash
+  files:
+    - path: output/antismash/test/NZ_CP069563.1.region001.gbk
+      contains: ['/tool="antismash"']
+    - path: output/antismash/test/NZ_CP069563.1.region002.gbk
+      contains: ['/tool="antismash"']
+    - path: output/antismash/test/css/bacteria.css
+      md5sum: 8b3c2f8b143d5245a5f42f55803c532c
+    - path: output/antismash/test/genome.gbk
+      contains: ['/tool="antismash"']
+    - path: output/antismash/test/genome.json
+      contains: ['{"version": "6.0.1", "input_file": "genome.fna", "records": [{"id": "NZ_CP069563.1", "seq": {"data":']
+    - path: output/antismash/test/genome.zip
+    - path: output/antismash/test/index.html
+      md5sum: de787e865c3a1eec143a19d2facb4de4
+    - path: output/antismash/test/js/antismash.js
+      md5sum: 58e90c3d783ae014cc3d51849bcb50a2
+    - path: output/antismash/test/js/jquery.js
+      md5sum: 397754ba49e9e0cf4e7c190da78dda05
+    - path: output/antismash/test/js/jquery.tablesorter.min.js
+      md5sum: 5e9e08cef4d1be0eaa538e6eb28809a7
+    - path: output/antismash/test/regions.js
+      contains: ['"seq_id": "NZ_CP069563.1"']
+    - path: output/antismash/test/test.log
+      contains: ["antiSMASH version: 6.0.1"]
+    - path: output/antismash/versions.yml
+      md5sum: 759431a43da33e2ef8e2d0ebd79a439b
+    - path: output/gunzip1/genome.fna
+      md5sum: dafd38f5454b54fbea38245d773062a5
+    - path: output/gunzip2/genome.gff
+      md5sum: 9b9c848b1946d43fa68128f4d6316052