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Piotr Faba 2020-03-06 14:23:32 +00:00
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name: Cutadapt name: Cutadapt
description: cutadapt removes adapter sequences from high-throughput sequencing reads description: cutadapt removes adapter sequences from high-throughput sequencing reads
keywords: keywords:
- Quality Control - Quality Control
- QC - QC
- Adapters - Adapters
tools: tools:
- fastqc: - fastqc:
description: | description: |
Cutadapt finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence Cutadapt finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence
from your high-throughput sequencing reads. from your high-throughput sequencing reads.
Cleaning your data in this way is often required: Reads from small-RNA sequencing contain the 3 Cleaning your data in this way is often required: Reads from small-RNA sequencing contain the 3
sequencing adapter because the read is longer than the molecule that is sequenced. Amplicon reads sequencing adapter because the read is longer than the molecule that is sequenced. Amplicon reads
start with a primer sequence. Poly-A tails are useful for pulling out RNA from your sample, but start with a primer sequence. Poly-A tails are useful for pulling out RNA from your sample, but
often you dont want them to be in your reads. often you dont want them to be in your reads.
homepage: https://cutadapt.readthedocs.io/en/stable/ homepage: https://cutadapt.readthedocs.io/en/stable/
documentation: https://cutadapt.readthedocs.io/en/stable/ documentation: https://cutadapt.readthedocs.io/en/stable/
input: input:
- - sample_id: -
type: string - sample_id:
description: Sample identifier type: string
- input_forward_fq: description: Sample identifier
type: file - reads:
description: Input FastQ forward read file of the pair type: file
- input_reverse_fq: description: Input FastQ file, or pair of files
type: file
description: Input FastQ reverse read file of the pair
output: output:
- - sample_id: -
type: string - sample_id:
description: Sample identifier type: string
- output_forward_fq: description: Sample identifier
type: file - reads:
description: Output FastQ forward read file of the pair type: file
- output_reverse_fq: description: trimmed FastQ file, or pair of files
type: file
description: Output FastQ reverse read file of the pair
authors: authors:
- @piotr-faba-ardigen - @piotr-faba-ardigen