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Merge branch 'master' into new-module-hap.py

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nvnieuwk 2022-05-04 14:01:02 +02:00 committed by GitHub
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6 changed files with 63 additions and 8 deletions
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4
modules/bowtie2/align/main.nf
View file

@ -29,6 +29,8 @@ process BOWTIE2_ALIGN {
def unaligned = save_unaligned ? "--un-gz ${prefix}.unmapped.fastq.gz" : ''
"""
INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'`
[ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed 's/.rev.1.bt2l//'`
[ -z "\$INDEX" ] && echo "BT2 index files not found" 1>&2 && exit 1
bowtie2 \\
-x \$INDEX \\
-U $reads \\
@ -49,6 +51,8 @@ process BOWTIE2_ALIGN {
def unaligned = save_unaligned ? "--un-conc-gz ${prefix}.unmapped.fastq.gz" : ''
"""
INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'`
[ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed 's/.rev.1.bt2l//'`
[ -z "\$INDEX" ] && echo "BT2 index files not found" 1>&2 && exit 1
bowtie2 \\
-x \$INDEX \\
-1 ${reads[0]} \\

3
modules/vardictjava/main.nf
View file

@ -10,8 +10,7 @@ process VARDICTJAVA {
'quay.io/biocontainers/vardict-java:1.8.3--hdfd78af_0' }"
input:
tuple val(meta), path(bam), path(bai)
path(bed)
tuple val(meta), path(bam), path(bai), path(bed)
tuple path(fasta), path(fasta_fai)
output:

13
tests/modules/bowtie2/align/nextflow.config
View file

@ -1,5 +1,16 @@
params {
force_large_index = false
}
process {
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
}
if (params.force_large_index) {
process {
withName: BOWTIE2_BUILD {
ext.args = '--large-index'
}
}
}

42
tests/modules/bowtie2/align/test.yml
View file

@ -39,3 +39,45 @@
md5sum: 52be6950579598a990570fbcf5372184
- path: ./output/bowtie2/bowtie2/genome.rev.2.bt2
md5sum: e3b4ef343dea4dd571642010a7d09597
- name: bowtie2 align single-end large-index
command: nextflow run ./tests/modules/bowtie2/align -entry test_bowtie2_align_single_end -c ./tests/config/nextflow.config -c ./tests/modules/bowtie2/align/nextflow.config --force_large_index
tags:
- bowtie2
- bowtie2/align
files:
- path: ./output/bowtie2/test.bam
- path: ./output/bowtie2/test.bowtie2.log
- path: ./output/bowtie2/bowtie2/genome.3.bt2l
md5sum: 8952b3e0b1ce9a7a5916f2e147180853
- path: ./output/bowtie2/bowtie2/genome.2.bt2l
md5sum: 22c284084784a0720989595e0c9461fd
- path: ./output/bowtie2/bowtie2/genome.1.bt2l
md5sum: 07d811cd4e350d56267183d2ac7023a5
- path: ./output/bowtie2/bowtie2/genome.4.bt2l
md5sum: c25be5f8b0378abf7a58c8a880b87626
- path: ./output/bowtie2/bowtie2/genome.rev.1.bt2l
md5sum: fda48e35925fb24d1c0785f021981e25
- path: ./output/bowtie2/bowtie2/genome.rev.2.bt2l
md5sum: 802c26d32b970e1b105032b7ce7348b4
- name: bowtie2 align paired-end large-index
command: nextflow run ./tests/modules/bowtie2/align -entry test_bowtie2_align_paired_end -c ./tests/config/nextflow.config -c ./tests/modules/bowtie2/align/nextflow.config --force_large_index
tags:
- bowtie2
- bowtie2/align
files:
- path: ./output/bowtie2/test.bam
- path: ./output/bowtie2/test.bowtie2.log
- path: ./output/bowtie2/bowtie2/genome.3.bt2l
md5sum: 8952b3e0b1ce9a7a5916f2e147180853
- path: ./output/bowtie2/bowtie2/genome.2.bt2l
md5sum: 22c284084784a0720989595e0c9461fd
- path: ./output/bowtie2/bowtie2/genome.1.bt2l
md5sum: 07d811cd4e350d56267183d2ac7023a5
- path: ./output/bowtie2/bowtie2/genome.4.bt2l
md5sum: c25be5f8b0378abf7a58c8a880b87626
- path: ./output/bowtie2/bowtie2/genome.rev.1.bt2l
md5sum: fda48e35925fb24d1c0785f021981e25
- path: ./output/bowtie2/bowtie2/genome.rev.2.bt2l
md5sum: 802c26d32b970e1b105032b7ce7348b4

7
tests/modules/vardictjava/main.nf
View file

@ -9,15 +9,14 @@ workflow test_vardictjava {
bam_input_ch = Channel.value([
[ id:'test' ], // meta map
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true),
file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true)
])
bed = Channel.value(file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true))
reference = Channel.value([
file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true),
file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true)
])
VARDICTJAVA ( bam_input_ch, bed, reference )
VARDICTJAVA ( bam_input_ch, reference )
}