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Merge branch 'master' into haplocheck

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Taniguti 2022-06-08 07:31:28 -03:00 committed by GitHub
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9 changed files with 121 additions and 73 deletions
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16
modules/tiddit/cov/main.nf
View file

@ -2,17 +2,17 @@ process TIDDIT_COV {
tag "$meta.id"
label 'process_low'
conda (params.enable_conda ? "bioconda::tiddit=2.12.1" : null)
conda (params.enable_conda ? "bioconda::tiddit=3.0.0" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/tiddit:2.12.1--py38h1773678_0' :
'quay.io/biocontainers/tiddit:2.12.1--py38h1773678_0' }"
'https://depot.galaxyproject.org/singularity/tiddit:3.0.0--py39h59fae87_1' :
'quay.io/biocontainers/tiddit:3.0.0--py39h59fae87_1' }"
input:
tuple val(meta), path(bam)
tuple val(meta), path(input)
path fasta
output:
tuple val(meta), path("*.tab"), optional: true, emit: cov
tuple val(meta), path("*.bed"), optional: true, emit: cov
tuple val(meta), path("*.wig"), optional: true, emit: wig
path "versions.yml" , emit: versions
@ -28,12 +28,12 @@ process TIDDIT_COV {
--cov \\
-o $prefix \\
$args \\
--bam $bam \\
--bam $input \\
$reference
cat <<-END_VERSIONS > versions.yml
"${task.process}":
tiddit: \$(echo \$(tiddit 2>&1) | sed 's/^.*TIDDIT-//; s/ .*\$//')
tiddit: \$(echo \$(tiddit 2>&1) | sed 's/^.*tiddit-//; s/ .*\$//')
END_VERSIONS
"""
@ -45,7 +45,7 @@ process TIDDIT_COV {
cat <<-END_VERSIONS > versions.yml
"${task.process}":
tiddit: \$(echo \$(tiddit 2>&1) | sed 's/^.*TIDDIT-//; s/ .*\$//')
tiddit: \$(echo \$(tiddit 2>&1) | sed 's/^.*tiddit-//; s/ .*\$//')
END_VERSIONS
"""
}

2
modules/tiddit/cov/meta.yml
View file

@ -19,7 +19,7 @@ input:
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- bam:
- input:
type: file
description: BAM/CRAM file
pattern: "*.{bam,cram}"

31
modules/tiddit/sv/main.nf
View file

@ -2,21 +2,20 @@ process TIDDIT_SV {
tag "$meta.id"
label 'process_medium'
conda (params.enable_conda ? "bioconda::tiddit=2.12.1" : null)
conda (params.enable_conda ? "bioconda::tiddit=3.0.0" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/tiddit:2.12.1--py38h1773678_0' :
'quay.io/biocontainers/tiddit:2.12.1--py38h1773678_0' }"
'https://depot.galaxyproject.org/singularity/tiddit:3.0.0--py39h59fae87_1' :
'quay.io/biocontainers/tiddit:3.0.0--py39h59fae87_1' }"
input:
tuple val(meta), path(bam)
tuple val(meta), path(input), path(input_index)
path fasta
path fai
path bwa_index
output:
tuple val(meta), path("*.vcf") , emit: vcf
tuple val(meta), path("*.ploidy.tab") , emit: ploidy
tuple val(meta), path("*.signals.tab"), emit: signals
path "versions.yml" , emit: versions
tuple val(meta), path("*.vcf") , emit: vcf
tuple val(meta), path("*.ploidies.tab"), emit: ploidy
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
@ -24,18 +23,19 @@ process TIDDIT_SV {
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def reference = fasta ? "--ref $fasta" : ""
"""
[[ -d $bwa_index ]] && for i in $bwa_index/*; do [[ -f $fasta && ! "\$i" =~ .*"$fasta".* ]] && ln -s \$i ${fasta}.\${i##*.} || ln -s \$i .; done
tiddit \\
--sv \\
$args \\
--bam $bam \\
$reference \\
--bam $input \\
--ref $fasta \\
-o $prefix
cat <<-END_VERSIONS > versions.yml
"${task.process}":
tiddit: \$(echo \$(tiddit 2>&1) | sed 's/^.*TIDDIT-//; s/ .*\$//')
tiddit: \$(echo \$(tiddit 2>&1) | sed 's/^.*tiddit-//; s/ .*\$//')
END_VERSIONS
"""
@ -43,12 +43,11 @@ process TIDDIT_SV {
def prefix = task.ext.prefix ?: "${meta.id}"
"""
touch ${prefix}.vcf
touch ${prefix}.ploidy.tab
touch ${prefix}.signals.tab
touch ${prefix}.ploidies.tab
cat <<-END_VERSIONS > versions.yml
"${task.process}":
tiddit: \$(echo \$(tiddit 2>&1) | sed 's/^.*TIDDIT-//; s/ .*\$//')
tiddit: \$(echo \$(tiddit 2>&1) | sed 's/^.*tiddit-//; s/ .*\$//')
END_VERSIONS
"""
}

20
modules/tiddit/sv/meta.yml
View file

@ -17,14 +17,22 @@ input:
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- input:
type: file
description: BAM/CRAM file
pattern: "*.{bam,cram}"
- index:
type: file
description: BAM/CRAM index file
pattern: "*.{bai,crai}"
- fasta:
type: file
description: Input FASTA file
pattern: "*.{fasta,fa}"
- fai:
- bwa_index:
type: file
description: FASTA index file
pattern: "*.{fai}"
description: BWA genome index files
pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}"
output:
- meta:
type: map
@ -38,11 +46,7 @@ output:
- ploidy:
type: file
description: tab
pattern: "*.{ploidy.tab}"
- signals:
type: file
description: tab
pattern: "*.{signals.tab}"
pattern: "*.{ploidies.tab}"
- versions:
type: file
description: File containing software versions

35
tests/modules/tiddit/cov/main.nf
View file

@ -2,22 +2,41 @@
nextflow.enable.dsl = 2
include { TIDDIT_COV } from '../../../../modules/tiddit/cov/main.nf'
include { TIDDIT_COV as TIDDIT_COV_BED } from '../../../../modules/tiddit/cov/main.nf'
include { TIDDIT_COV as TIDDIT_COV_WIG } from '../../../../modules/tiddit/cov/main.nf'
workflow test_tiddit_cov {
workflow test_tiddit_cov_cram_bed {
input = [ [ id:'test', single_end:false ], // meta map
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true) ]
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
fasta = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
TIDDIT_COV ( input, fasta )
TIDDIT_COV_BED ( input, fasta )
}
workflow test_tiddit_cov_no_ref {
workflow test_tiddit_cov_bam_bed {
input = [ [ id:'test', single_end:false ], // meta map
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
TIDDIT_COV ( input, [] )
TIDDIT_COV_BED ( input, [] )
}
workflow test_tiddit_cov_cram_wig {
input = [ [ id:'test', single_end:false ], // meta map
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true) ]
fasta = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
TIDDIT_COV_WIG ( input, fasta )
}
workflow test_tiddit_cov_bam_wig {
input = [ [ id:'test', single_end:false ], // meta map
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
TIDDIT_COV_WIG ( input, [] )
}

4
tests/modules/tiddit/cov/nextflow.config
View file

@ -2,4 +2,8 @@ process {
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
withName: TIDDIT_COV_WIG {
ext.args = '-w'
}
}

34
tests/modules/tiddit/cov/test.yml
View file

@ -1,17 +1,35 @@
- name: tiddit cov test_tiddit_cov
command: nextflow run ./tests/modules/tiddit/cov -entry test_tiddit_cov -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/cov/nextflow.config
- name: tiddit cov test_tiddit_cov_cram_bed
command: nextflow run ./tests/modules/tiddit/cov -entry test_tiddit_cov_cram_bed -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/cov/nextflow.config
tags:
- tiddit
- tiddit/cov
files:
- path: output/tiddit/test.tab
md5sum: f7974948f809f94879d8a60b726194f5
- path: output/tiddit/test.bed
md5sum: 3b1a28c62a5f25bbba77c1042e9abdf7
- name: tiddit cov test_tiddit_cov_no_ref
command: nextflow run ./tests/modules/tiddit/cov -entry test_tiddit_cov_no_ref -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/cov/nextflow.config
- name: tiddit cov test_tiddit_cov_bam_bed
command: nextflow run ./tests/modules/tiddit/cov -entry test_tiddit_cov_bam_bed -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/cov/nextflow.config
tags:
- tiddit
- tiddit/cov
files:
- path: output/tiddit/test.tab
md5sum: f7974948f809f94879d8a60b726194f5
- path: output/tiddit/test.bed
md5sum: 9d1474f1c7c6516205254077087bb026
- name: tiddit cov test_tiddit_cov_cram_wig
command: nextflow run ./tests/modules/tiddit/cov -entry test_tiddit_cov_cram_wig -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/cov/nextflow.config
tags:
- tiddit
- tiddit/cov
files:
- path: output/tiddit/test.wig
md5sum: ca3645fd0c3491c86c075c91d16d57c4
- name: tiddit cov test_tiddit_cov_bam_wig
command: nextflow run ./tests/modules/tiddit/cov -entry test_tiddit_cov_bam_wig -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/cov/nextflow.config
tags:
- tiddit
- tiddit/cov
files:
- path: output/tiddit/test.wig
md5sum: 44bea2ac6a56774738e65773065da670

28
tests/modules/tiddit/sv/main.nf
View file

@ -2,25 +2,33 @@
nextflow.enable.dsl = 2
include { BWA_INDEX } from '../../../../modules/bwa/index/main.nf'
include { TIDDIT_SV } from '../../../../modules/tiddit/sv/main.nf'
workflow test_tiddit_sv {
input = [
workflow test_tiddit_sv_bam {
input = [
[ id:'test' ], // meta map
[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ],
[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true) ]
]
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
TIDDIT_SV ( input, fasta, fai )
BWA_INDEX( fasta )
TIDDIT_SV ( input, fasta, BWA_INDEX.out.index)
}
workflow test_tiddit_sv_no_ref {
input = [
workflow test_tiddit_sv_cram {
input = [
[ id:'test' ], // meta map
[ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
[ file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram'], checkIfExists: true) ],
[ file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_cram_crai'], checkIfExists: true) ]
]
TIDDIT_SV ( input, [], [] )
fasta = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
BWA_INDEX( fasta )
TIDDIT_SV ( input, fasta, BWA_INDEX.out.index)
}

24
tests/modules/tiddit/sv/test.yml
View file

@ -1,25 +1,21 @@
- name: tiddit sv
command: nextflow run ./tests/modules/tiddit/sv -entry test_tiddit_sv -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/sv/nextflow.config
- name: tiddit sv test_tiddit_sv_bam
command: nextflow run ./tests/modules/tiddit/sv -entry test_tiddit_sv_bam -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/sv/nextflow.config
tags:
- tiddit
- tiddit/sv
files:
- path: output/tiddit/test.ploidy.tab
md5sum: 45e050b0e204f0a5a3a99627cc440eaa
- path: output/tiddit/test.signals.tab
md5sum: dab4b2fec4ddf8eb1c23005b0770150e
- path: output/tiddit/test.ploidies.tab
md5sum: 6319d3611f7b6b94425a184d274b3dfc
- path: output/tiddit/test.vcf
md5sum: bdce14ae8292bf3deb81f6f255baf859
md5sum: 41d3f8746f0420f894104321b7e64f67
- name: tiddit sv no ref
command: nextflow run ./tests/modules/tiddit/sv -entry test_tiddit_sv_no_ref -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/sv/nextflow.config
- name: tiddit sv test_tiddit_sv_cram
command: nextflow run ./tests/modules/tiddit/sv -entry test_tiddit_sv_cram -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/sv/nextflow.config
tags:
- tiddit
- tiddit/sv
files:
- path: output/tiddit/test.ploidy.tab
md5sum: 45e050b0e204f0a5a3a99627cc440eaa
- path: output/tiddit/test.signals.tab
md5sum: dab4b2fec4ddf8eb1c23005b0770150e
- path: output/tiddit/test.ploidies.tab
md5sum: f1162a940ddc8b963f6e0e506bb5c136
- path: output/tiddit/test.vcf
md5sum: 3d0e83a8199b2bdb81cfe3e6b12bf64b
md5sum: 34db59578991285d6b62dc1500272fca