From d8028dc1c3ef64c2ee3494ce65d4f4a76c42bde9 Mon Sep 17 00:00:00 2001 From: Ramprasad Neethiraj <20065894+ramprasadn@users.noreply.github.com> Date: Thu, 3 Mar 2022 17:40:16 +0100 Subject: [PATCH] Add picard/sortvcf (#1370) * sortvcf * add files * update meta * update java mem * update documentation link * remove todo * review suggestions * fix test.yml * fix conda error * fix version code --- modules/picard/sortvcf/main.nf | 49 ++++++++++++++++++++ modules/picard/sortvcf/meta.yml | 40 ++++++++++++++++ tests/config/pytest_modules.yml | 4 ++ tests/modules/picard/sortvcf/main.nf | 18 +++++++ tests/modules/picard/sortvcf/nextflow.config | 5 ++ tests/modules/picard/sortvcf/test.yml | 7 +++ 6 files changed, 123 insertions(+) create mode 100644 modules/picard/sortvcf/main.nf create mode 100644 modules/picard/sortvcf/meta.yml create mode 100644 tests/modules/picard/sortvcf/main.nf create mode 100644 tests/modules/picard/sortvcf/nextflow.config create mode 100644 tests/modules/picard/sortvcf/test.yml diff --git a/modules/picard/sortvcf/main.nf b/modules/picard/sortvcf/main.nf new file mode 100644 index 00000000..0f10c1ab --- /dev/null +++ b/modules/picard/sortvcf/main.nf @@ -0,0 +1,49 @@ +process PICARD_SORTVCF { + tag "$meta.id" + label 'process_medium' + + conda (params.enable_conda ? "bioconda::picard=2.26.10" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' : + 'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }" + + input: + tuple val(meta), path(vcf) + path reference + path sequence_dict + + output: + tuple val(meta), path("*_sorted.vcf.gz"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seq_dict = sequence_dict ? "-SEQUENCE_DICTIONARY $sequence_dict" : "" + def reference = reference ? "-REFERENCE_SEQUENCE $reference" : "" + def avail_mem = 3 + if (!task.memory) { + log.info '[Picard SortVcf] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + + """ + picard \\ + SortVcf \\ + -Xmx${avail_mem}g \\ + --INPUT $vcf \\ + $args \\ + $seq_dict \\ + $reference \\ + --OUTPUT ${prefix}_sorted.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + picard: \$(picard SortVcf --version 2>&1 | grep -o 'Version:.*' | cut -f2- -d:) + END_VERSIONS + """ +} diff --git a/modules/picard/sortvcf/meta.yml b/modules/picard/sortvcf/meta.yml new file mode 100644 index 00000000..a2b46d5a --- /dev/null +++ b/modules/picard/sortvcf/meta.yml @@ -0,0 +1,40 @@ +name: picard_sortvcf +description: Sorts vcf files +keywords: + - sort + - vcf +tools: + - picard: + description: Java tools for working with NGS data in the BAM/CRAM/SAM and VCF format + homepage: https://broadinstitute.github.io/picard/ + documentation: https://broadinstitute.github.io/picard/command-line-overview.html#SortVcf + licence: ['MIT'] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF file + pattern: "*.{vcf,vcf.gz}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: Sorted VCF file + pattern: "*.{vcf}" + +authors: + - "@ramprasadn" diff --git a/tests/config/pytest_modules.yml b/tests/config/pytest_modules.yml index de03a379..553128de 100644 --- a/tests/config/pytest_modules.yml +++ b/tests/config/pytest_modules.yml @@ -1249,6 +1249,10 @@ picard/sortsam: - modules/picard/sortsam/** - tests/modules/picard/sortsam/** +picard/sortvcf: + - modules/picard/sortvcf/** + - tests/modules/picard/sortvcf/** + pirate: - modules/pirate/** - tests/modules/pirate/** diff --git a/tests/modules/picard/sortvcf/main.nf b/tests/modules/picard/sortvcf/main.nf new file mode 100644 index 00000000..a88c69fc --- /dev/null +++ b/tests/modules/picard/sortvcf/main.nf @@ -0,0 +1,18 @@ +#!/usr/bin/env nextflow + +nextflow.enable.dsl = 2 + +include { PICARD_SORTVCF } from '../../../../modules/picard/sortvcf/main.nf' + +workflow test_picard_sortvcf { + + input = [ [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true) + ] + + fasta = [ file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + + dict = [ file(params.test_data['sarscov2']['genome']['genome_dict'], checkIfExists: true) ] + + PICARD_SORTVCF ( input, fasta, dict ) +} diff --git a/tests/modules/picard/sortvcf/nextflow.config b/tests/modules/picard/sortvcf/nextflow.config new file mode 100644 index 00000000..8730f1c4 --- /dev/null +++ b/tests/modules/picard/sortvcf/nextflow.config @@ -0,0 +1,5 @@ +process { + + publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } + +} diff --git a/tests/modules/picard/sortvcf/test.yml b/tests/modules/picard/sortvcf/test.yml new file mode 100644 index 00000000..26271077 --- /dev/null +++ b/tests/modules/picard/sortvcf/test.yml @@ -0,0 +1,7 @@ +- name: picard sortvcf + command: nextflow run ./tests/modules/picard/sortvcf -entry test_picard_sortvcf -c ./tests/config/nextflow.config -c ./tests/modules/picard/sortvcf/nextflow.config + tags: + - picard + - picard/sortvcf + files: + - path: output/picard/test_sorted.vcf.gz