diff --git a/modules/meryl/count/main.nf b/modules/meryl/count/main.nf new file mode 100644 index 00000000..f7a2811d --- /dev/null +++ b/modules/meryl/count/main.nf @@ -0,0 +1,37 @@ +process MERYL_COUNT { + tag "$meta.id" + label 'process_medium' + + conda (params.enable_conda ? "bioconda::meryl=1.3" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/meryl:1.3--h87f3376_1': + 'quay.io/biocontainers/meryl:1.3--h87f3376_1' }" + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.meryldb"), emit: meryl_db + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + for READ in $reads; do + meryl count \\ + threads=$task.cpus \\ + $args \\ + $reads \\ + output read.\${READ%.f*}.meryldb + done + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + meryl: \$( meryl --version |& sed 's/meryl //' ) + END_VERSIONS + """ +} diff --git a/modules/meryl/count/meta.yml b/modules/meryl/count/meta.yml new file mode 100644 index 00000000..854f8759 --- /dev/null +++ b/modules/meryl/count/meta.yml @@ -0,0 +1,43 @@ +name: "meryl_count" +description: A genomic k-mer counter (and sequence utility) with nice features. +keywords: + - k-mer + - count +tools: + - "meryl": + description: "A genomic k-mer counter (and sequence utility) with nice features. " + homepage: "https://github.com/marbl/meryl" + documentation: "https://meryl.readthedocs.io/en/latest/quick-start.html" + tool_dev_url: "https://github.com/marbl/meryl" + doi: "" + licence: "['GPL']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - meryl_db: + type: directory + description: A Meryl k-mer database + pattern: "*.meryldb" + +authors: + - "@mahesh-panchal" diff --git a/tests/config/pytest_modules.yml b/tests/config/pytest_modules.yml index b9c67363..ec7fc321 100644 --- a/tests/config/pytest_modules.yml +++ b/tests/config/pytest_modules.yml @@ -1214,6 +1214,10 @@ meningotype: - modules/meningotype/** - tests/modules/meningotype/** +meryl/count: + - modules/meryl/count/** + - tests/modules/meryl/count/** + metabat2/jgisummarizebamcontigdepths: - modules/metabat2/jgisummarizebamcontigdepths/** - tests/modules/metabat2/jgisummarizebamcontigdepths/** diff --git a/tests/modules/meryl/count/main.nf b/tests/modules/meryl/count/main.nf new file mode 100644 index 00000000..3c9a64c7 --- /dev/null +++ b/tests/modules/meryl/count/main.nf @@ -0,0 +1,28 @@ +#!/usr/bin/env nextflow + +nextflow.enable.dsl = 2 + +include { MERYL_COUNT } from '../../../../modules/meryl/count/main.nf' + +workflow test_meryl_count_single_end { + + input = [ + [ id:'test' , single_end: true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + + MERYL_COUNT ( input ) +} + +workflow test_meryl_count_paired_end { + + input = [ + [ id:'test' , single_end: false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + + MERYL_COUNT ( input ) +} diff --git a/tests/modules/meryl/count/nextflow.config b/tests/modules/meryl/count/nextflow.config new file mode 100644 index 00000000..6d899c50 --- /dev/null +++ b/tests/modules/meryl/count/nextflow.config @@ -0,0 +1,6 @@ +process { + + publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } + ext.args = 'k=21' + +} diff --git a/tests/modules/meryl/count/test.yml b/tests/modules/meryl/count/test.yml new file mode 100644 index 00000000..6291a62f --- /dev/null +++ b/tests/modules/meryl/count/test.yml @@ -0,0 +1,17 @@ +- name: meryl count test_meryl_count_single_end + command: nextflow run tests/modules/meryl/count -entry test_meryl_count_single_end -c tests/config/nextflow.config + tags: + - meryl/count + - meryl + files: + - path: output/meryl/versions.yml + md5sum: 5fe537d873925ccbcc4edf0983e9eda0 + +- name: meryl count test_meryl_count_paired_end + command: nextflow run tests/modules/meryl/count -entry test_meryl_count_paired_end -c tests/config/nextflow.config + tags: + - meryl/count + - meryl + files: + - path: output/meryl/versions.yml + md5sum: 4961f13cfb60ba8764ed666e70dbf12c