From 8f546a1f956d6750374f30e79e9c04029227f254 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Tue, 9 Feb 2021 10:26:35 -0800 Subject: [PATCH 01/45] Add ivar consensus --- software/ivar/consensus/functions.nf | 59 ++++++++++++++++++ software/ivar/consensus/main.nf | 46 ++++++++++++++ .../bam/test-sc2-artic-v3-sorted-trimmed.bam | Bin 0 -> 83301 bytes tests/data/bam/test-sc2-artic-v3.bam | Bin 0 -> 594300 bytes tests/software/ivar/consensus/main.nf | 19 ++++++ tests/software/ivar/consensus/test.yml | 13 ++++ 6 files changed, 137 insertions(+) create mode 100644 software/ivar/consensus/functions.nf create mode 100644 software/ivar/consensus/main.nf create mode 100644 tests/data/bam/test-sc2-artic-v3-sorted-trimmed.bam create mode 100644 tests/data/bam/test-sc2-artic-v3.bam create mode 100644 tests/software/ivar/consensus/main.nf create mode 100644 tests/software/ivar/consensus/test.yml diff --git a/software/ivar/consensus/functions.nf b/software/ivar/consensus/functions.nf new file mode 100644 index 00000000..d25eea86 --- /dev/null +++ b/software/ivar/consensus/functions.nf @@ -0,0 +1,59 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf new file mode 100644 index 00000000..54ea8f8a --- /dev/null +++ b/software/ivar/consensus/main.nf @@ -0,0 +1,46 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +process IVAR_CONSENSUS { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } + + conda (params.enable_conda ? "bioconda::ivar=1.3.1" : null) + + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/ivar:1.3.1--h089eab3_0" + } else { + container "quay.io/biocontainers/ivar:1.3.1--h089eab3_0" + } + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.fa") , emit: cns_seq + tuple val(meta), path("*.qual.txt"), emit: cns_qual + tuple val(meta), path("*_mpileup.txt"), emit: mpileup + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + samtools mpileup \\ + -aa -A -d 0 -Q 0 \\ + $options.args2 \\ + $bam | \\ + tee ${prefix}_mpileup.txt | \\ + ivar consensus \\ + $options.args \\ + -p $prefix + + ivar version | head -n1 2>&1 | sed 's/^.*iVar version //g' > ${software}.version.txt + """ +} diff --git a/tests/data/bam/test-sc2-artic-v3-sorted-trimmed.bam b/tests/data/bam/test-sc2-artic-v3-sorted-trimmed.bam new file mode 100644 index 0000000000000000000000000000000000000000..e1b2ead44e77f62e1e95730ab98def06c2cd0dc8 GIT binary patch literal 83301 zcmV)AK*YZviwFb&00000{{{d;LjnM70j-eDZh}A*g~xO?_9f;8?t}|Og%xd0e-fou zOjkB$P;5w~w8I3GzGPpiGa#k@?OYJJ9HHKw&z(+H<&)qr=dSf3WYQp*F!g$RBF5xbP`rS&9!)+ zV;>~qU_kFiMrK)(F_%OKFs5JwF1iVsq3c`S6=LpH}l`rmxYqR6G% z(RXB{B&!-e`!DSoAKBXHY;~aX-Qpw9_PjpYE&_Tzw=qjgAwkJBGhXIVlO1iL(MDiq z%OKrhmo^Bg(j}BmT_`@a(AD6xS{$`<&bG2Ia?X>dbPrA2-WT)i5JIl?*PfgFC_;V# zcYY-zd;SRkqVWd zRN{nGA*vj>@O{5_-prd_&+M)v^JMM3eKTW!Ki}Wq?|c5U#>N-Rns(~KOZ6q~b?p{k z`vlf*!`1b=Zs2HdoPaqjCBuHL^PoA<`{uxGj*?NWf$F2JK1`FXdeW$klKNh)-xzM` z?RHz&EjI9Ypq2>(V;kl+4EV6f2j<^lW?DM74SpM@wqaY?vTXQ0Hom@nN!uRWjRI}^ z(%mT9*7`Ru-MW4AlJ@H5yF1@`>-&y&yZ@G^{Z@kZ6$))%+vaP3g*7sFJkRNBJAiEW zIZYcRwfaa;wi@-pR(~`a?$x&RPGZ(zI?y|PK-llp8f)$L^X>MgL4fH%6gFENT+1Xh z)-Yhx8*QtG8nD(vs}`Wp0h4XR0|YGju_<7Sg9$?dCJZQ;Z57N<6fm!A=Or-TAaZg; zFQi~@EFA)-O(Y`=21st3bY^oIa2kPTE0v3Fb9()V(CahipI7PqMS)(uDA9YFFh2_Y z9^?L6IqvH)kfKjnvd06N_~$y z)*hwQS1J9GM5zb_E?@gQta-$-_Ix+e{sXfv5IRu?Bnq31N<-$iN}&hPO|Ao~(YbL^T`L<{+f*vym{SI(}qQv{G_(5rrb}sLb zpFWJ89mk1@6k&ba@dl2|eAZ)Cnja_{w@+QIkS3Y;0uM4oB25xQ$_9`Y$)GkNiINzP zNu(RINjEVvQ|6?2F;5$X-*u?pWg2-|#qi4ls~JidUV>{JI#En9yj>1M%`i+;pIv$K zE;w{42uL-1y`(cWl?8hY}eUK{EC#@?Xb z>2Ea~y9gPEQORrA6GomJl6(*K>*}yJt@JIANaVf3M|vy05(~@$?4e zX%Gg?SCRUsBv03fXECO02TmD5x~P{`aRC92D0wR8v+Qd&$#Fg6bb0WMqBeOSQzZHeIPJOhuJa{a9=@00f8 z)Jn&>dLo)sPaG7m|AK_(0!_wUpGUTj4r70F9uOi8r`jJd?Z2SXd{v48%bezioaTSP z+C!!?+UGm~lx);=>VRQXAJvgwYi=b3TXR&LRuL;=sWA2}Y)Q2iN{~)QrNv_bZNbDqYuLl)A;U}qS%JOq9)dd4pRE6RpDj7%e z|F5Wses@Ae^pqod3Tta{zvp#Bis+Y~I__nUYN*R=seh~TJg3NWUrQtnCG)_KTxRj779meGt_#iiR5zdO;0bpMhOl6d z=ERQ2_>-voc~oG*FG&0$9hIo(I+Q=8C3FaX*3zoj&}Tp|{J1BzQ(ic{M3&9;pRlH# zAkAq(_p`Orfnuj5lSPL(Gi!#F-%9J@SyKK@NcrX0a3=7Gu?pWe#eF(f))AfJ;$exw zW$E@p*+`$N()^XCO7n`OgjeWRLkJCz&v{B(N}6d#isp$CfdLER)M z{UG#?+Mp^IP3|Ov`mq9OpKhcGNIe26BAY7Ipq8dUB86Yo>{)bQD3v_R@Kh>)RzPJw z)$MgXw^usX&FB*yEtXNG4qng-t2%R20F07umCbd(ETH?oMECay-G0|$9{AwoZ0_o! zlrV6{TSt&Eq~dbAthwA}&E@+l@Lv~L%2f&YH3~d*Sd#xy*|hMYB(@eic4CScV~Gxo zfz39R6CCs~5wEHnCKg3CRJx=inC(>hlRA~t$`um?_D#6&d%bRSJj#_trHDhcwS~KqY0LwPqvJ9CfA3fYY3bKXGA=6}H>{fK!Is-DSr-ix29{RdPc6$-C0r=Eeh#|EB1eDFz<_6NKC|jhV z2Re>UlhYGx(BA`RW*DpI&aG}h@v*Xj4f@wG;(x(fS#O?Rp`MWFlC`>y^s`O)Xst9? 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zO6TVr1%cKX->$C7ZC>;8l_6wF7C9Cc z0XGD|GtMxg1Q=jYMs1t#apq=H0z8MzU4Wx`u;?GIZP9PS2~!~`>1eg4>P(&~LCdk8 zj0Bm(xAp|L1qAy!Gwi<$`Au*>`!6XoT2-QP6x@(ypFUo+3;sW`;7`A=#{d8yiwFb& a00000{{{d;LjnLB00RI3000000001vfq_>5 literal 0 HcmV?d00001 diff --git a/tests/software/ivar/consensus/main.nf b/tests/software/ivar/consensus/main.nf new file mode 100644 index 00000000..70ffe295 --- /dev/null +++ b/tests/software/ivar/consensus/main.nf @@ -0,0 +1,19 @@ +#!/usr/bin/env nextflow + +nextflow.enable.dsl = 2 +params.publish_dir_mode = 'link' +params.enable_conda = false + +include { IVAR_CONSENSUS } from '../../../../software/ivar/consensus/main.nf' addParams([:]) + +workflow test_ivar_consensus { + + bam = file("${launchDir}/tests/data/bam/test-sc2-artic-v3.bam", checkIfExists: true) + + def input = [] + input = [ [ id:'test'], + file("${launchDir}/tests/data/bam/test-sc2-artic-v3-sorted-trimmed.bam", checkIfExists: true) ] + + main: + IVAR_CONSENSUS ( input ) +} \ No newline at end of file diff --git a/tests/software/ivar/consensus/test.yml b/tests/software/ivar/consensus/test.yml new file mode 100644 index 00000000..bd4caa63 --- /dev/null +++ b/tests/software/ivar/consensus/test.yml @@ -0,0 +1,13 @@ +- name: Run ivar consensus test workflow + command: nextflow run ./tests/software/ivar/consensus -profile docker -entry test_ivar_consensus -c tests/config/nextflow.config + tags: + - ivar + - ivar_consensus + files: + - path: output/ivar/test.fa + md5sum: d791e777b669bd40f6074b5566722a47 + - path: output/ivar/test.qual.txt + md5sum: 723126475ec74adfd876e2b47ffe35a8 + - path: output/ivar/test_mpileup.txt + md5sum: d1c0ef3978bec1c09c549f884ed5b76a + From 884c4658b87fda9b7ff1a830a9b2def25041557e Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Wed, 10 Feb 2021 10:00:22 -0800 Subject: [PATCH 02/45] Make mpileup saving optional Co-authored-by: Harshil Patel --- software/ivar/consensus/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf index 54ea8f8a..bf326205 100644 --- a/software/ivar/consensus/main.nf +++ b/software/ivar/consensus/main.nf @@ -36,7 +36,7 @@ process IVAR_CONSENSUS { -aa -A -d 0 -Q 0 \\ $options.args2 \\ $bam | \\ - tee ${prefix}_mpileup.txt | \\ + $save_mpileup \\ ivar consensus \\ $options.args \\ -p $prefix From 362a630f8bb8e01a29dc4bb463efc6c725eaa74b Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Wed, 10 Feb 2021 10:00:54 -0800 Subject: [PATCH 03/45] Change suffix of mpileup ouput file Co-authored-by: Harshil Patel --- software/ivar/consensus/main.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf index bf326205..f1e6a6d5 100644 --- a/software/ivar/consensus/main.nf +++ b/software/ivar/consensus/main.nf @@ -23,9 +23,9 @@ process IVAR_CONSENSUS { tuple val(meta), path(bam) output: - tuple val(meta), path("*.fa") , emit: cns_seq - tuple val(meta), path("*.qual.txt"), emit: cns_qual - tuple val(meta), path("*_mpileup.txt"), emit: mpileup + tuple val(meta), path("*.fa") , emit: fasta + tuple val(meta), path("*.qual.txt"), emit: qual + tuple val(meta), path("*.mpileup") , emit: mpileup path "*.version.txt" , emit: version script: From 06770d5f02bd249427105b480159840ac2423569 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Wed, 10 Feb 2021 10:01:25 -0800 Subject: [PATCH 04/45] Specify the exact bioconda recipe build Co-authored-by: Harshil Patel --- software/ivar/consensus/main.nf | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf index f1e6a6d5..cd58c897 100644 --- a/software/ivar/consensus/main.nf +++ b/software/ivar/consensus/main.nf @@ -11,8 +11,7 @@ process IVAR_CONSENSUS { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } - conda (params.enable_conda ? "bioconda::ivar=1.3.1" : null) - + conda (params.enable_conda ? "bioconda::ivar=1.3.1=h089eab3_0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/ivar:1.3.1--h089eab3_0" } else { From e9aa037e7bf9094757ac5e9d16c2bc1beac771ec Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Wed, 10 Feb 2021 10:02:06 -0800 Subject: [PATCH 05/45] Add function to enable output of mpileup Co-authored-by: Harshil Patel --- software/ivar/consensus/main.nf | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf index cd58c897..a0288365 100644 --- a/software/ivar/consensus/main.nf +++ b/software/ivar/consensus/main.nf @@ -28,8 +28,9 @@ process IVAR_CONSENSUS { path "*.version.txt" , emit: version script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def save_mpileup = params.save_mpileup ? "tee ${prefix}.mpileup |" : "" """ samtools mpileup \\ -aa -A -d 0 -Q 0 \\ From 1f6419f8f594eaf4711a304f3d1b0f9140ae58de Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Wed, 10 Feb 2021 10:55:24 -0800 Subject: [PATCH 06/45] Fix indentation Co-authored-by: Harshil Patel --- tests/software/ivar/consensus/main.nf | 6 ++---- 1 file changed, 2 insertions(+), 4 deletions(-) diff --git a/tests/software/ivar/consensus/main.nf b/tests/software/ivar/consensus/main.nf index 70ffe295..8b579f5d 100644 --- a/tests/software/ivar/consensus/main.nf +++ b/tests/software/ivar/consensus/main.nf @@ -13,7 +13,5 @@ workflow test_ivar_consensus { def input = [] input = [ [ id:'test'], file("${launchDir}/tests/data/bam/test-sc2-artic-v3-sorted-trimmed.bam", checkIfExists: true) ] - - main: - IVAR_CONSENSUS ( input ) -} \ No newline at end of file + IVAR_CONSENSUS ( input ) +} From ffa9cf85b1d3e49406fb4b915268c44f59af0490 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Wed, 10 Feb 2021 12:03:50 -0800 Subject: [PATCH 07/45] Clean up header params I used these for testing and forgot to remove them Co-authored-by: Harshil Patel --- tests/software/ivar/consensus/main.nf | 2 -- 1 file changed, 2 deletions(-) diff --git a/tests/software/ivar/consensus/main.nf b/tests/software/ivar/consensus/main.nf index 8b579f5d..146a7b72 100644 --- a/tests/software/ivar/consensus/main.nf +++ b/tests/software/ivar/consensus/main.nf @@ -1,8 +1,6 @@ #!/usr/bin/env nextflow nextflow.enable.dsl = 2 -params.publish_dir_mode = 'link' -params.enable_conda = false include { IVAR_CONSENSUS } from '../../../../software/ivar/consensus/main.nf' addParams([:]) From 68de6fef0d8d313689d284ed8a524e4610833262 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Wed, 10 Feb 2021 12:04:15 -0800 Subject: [PATCH 08/45] Fix indentation Co-authored-by: Harshil Patel --- tests/software/ivar/consensus/main.nf | 2 -- 1 file changed, 2 deletions(-) diff --git a/tests/software/ivar/consensus/main.nf b/tests/software/ivar/consensus/main.nf index 146a7b72..5fdc851e 100644 --- a/tests/software/ivar/consensus/main.nf +++ b/tests/software/ivar/consensus/main.nf @@ -5,8 +5,6 @@ nextflow.enable.dsl = 2 include { IVAR_CONSENSUS } from '../../../../software/ivar/consensus/main.nf' addParams([:]) workflow test_ivar_consensus { - - bam = file("${launchDir}/tests/data/bam/test-sc2-artic-v3.bam", checkIfExists: true) def input = [] input = [ [ id:'test'], From 40bbf94ef96eb67e895d4c7c6a150eabbb33a127 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Wed, 10 Feb 2021 12:04:46 -0800 Subject: [PATCH 09/45] Update filename for outputted mpileup file in test Co-authored-by: Harshil Patel --- tests/software/ivar/consensus/test.yml | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/tests/software/ivar/consensus/test.yml b/tests/software/ivar/consensus/test.yml index bd4caa63..8599707d 100644 --- a/tests/software/ivar/consensus/test.yml +++ b/tests/software/ivar/consensus/test.yml @@ -8,6 +8,5 @@ md5sum: d791e777b669bd40f6074b5566722a47 - path: output/ivar/test.qual.txt md5sum: 723126475ec74adfd876e2b47ffe35a8 - - path: output/ivar/test_mpileup.txt + - path: output/ivar/test.mpileup md5sum: d1c0ef3978bec1c09c549f884ed5b76a - From df9125b46a696ddf4b49fb15152a2e4c1da93683 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Wed, 10 Feb 2021 12:05:01 -0800 Subject: [PATCH 10/45] Fix test name Co-authored-by: Harshil Patel --- tests/software/ivar/consensus/test.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/software/ivar/consensus/test.yml b/tests/software/ivar/consensus/test.yml index 8599707d..4e799629 100644 --- a/tests/software/ivar/consensus/test.yml +++ b/tests/software/ivar/consensus/test.yml @@ -1,4 +1,4 @@ -- name: Run ivar consensus test workflow +- name: ivar consensus command: nextflow run ./tests/software/ivar/consensus -profile docker -entry test_ivar_consensus -c tests/config/nextflow.config tags: - ivar From 6e8b63fc263741b32a61dfb244e03e4b6c8dbd5f Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 12 Feb 2021 17:22:07 +0100 Subject: [PATCH 11/45] Add cat fastq module --- .github/filters.yml | 4 +++ software/cat/fastq/functions.nf | 59 +++++++++++++++++++++++++++++++ software/cat/fastq/main.nf | 46 ++++++++++++++++++++++++ software/cat/fastq/meta.yml | 55 ++++++++++++++++++++++++++++ tests/software/cat/fastq/main.nf | 27 ++++++++++++++ tests/software/cat/fastq/test.yml | 21 +++++++++++ 6 files changed, 212 insertions(+) create mode 100644 software/cat/fastq/functions.nf create mode 100644 software/cat/fastq/main.nf create mode 100644 software/cat/fastq/meta.yml create mode 100644 tests/software/cat/fastq/main.nf create mode 100644 tests/software/cat/fastq/test.yml diff --git a/.github/filters.yml b/.github/filters.yml index 74156e9d..f63a162d 100644 --- a/.github/filters.yml +++ b/.github/filters.yml @@ -92,6 +92,10 @@ bwa_mem: - software/bwa/mem/** - tests/software/bwa/mem/** +cat_fastq: + - software/cat/fastq/** + - tests/software/cat/fastq/** + cutadapt: - software/cutadapt/** - tests/software/cutadapt/** diff --git a/software/cat/fastq/functions.nf b/software/cat/fastq/functions.nf new file mode 100644 index 00000000..d25eea86 --- /dev/null +++ b/software/cat/fastq/functions.nf @@ -0,0 +1,59 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/software/cat/fastq/main.nf b/software/cat/fastq/main.nf new file mode 100644 index 00000000..44a06bb8 --- /dev/null +++ b/software/cat/fastq/main.nf @@ -0,0 +1,46 @@ +// Import generic module functions +include { initOptions; saveFiles } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +process CAT_FASTQ { + tag "$meta.id" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', publish_id:meta.id) } + + conda (params.enable_conda ? "conda-forge::sed=4.7=h1bed415_1000" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" + } else { + container "biocontainers/biocontainers:v1.2.0_cv1" + } + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.merged.fastq.gz"), emit: reads + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def readList = reads.collect{ it.toString() } + if (meta.single_end) { + if (readList.size > 1) { + """ + cat ${readList.sort().join(' ')} > ${prefix}.merged.fastq.gz + """ + } + } else { + if (readList.size > 2) { + def read1 = [] + def read2 = [] + readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v } + """ + cat ${read1.sort().join(' ')} > ${prefix}_1.merged.fastq.gz + cat ${read2.sort().join(' ')} > ${prefix}_2.merged.fastq.gz + """ + } + } +} diff --git a/software/cat/fastq/meta.yml b/software/cat/fastq/meta.yml new file mode 100644 index 00000000..eda6dff7 --- /dev/null +++ b/software/cat/fastq/meta.yml @@ -0,0 +1,55 @@ +name: cat_fastq +description: Concatenates fastq files +keywords: + - fastq + - concatenate +tools: + - cat: + description: | + The cat utility reads files sequentially, writing them to the standard output. + documentation: https://www.gnu.org/software/coreutils/manual/html_node/cat-invocation.html +params: + - outdir: + type: string + description: | + The pipeline's output directory. By default, the module will + output files into `$params.outdir/` + - publish_dir_mode: + type: string + description: | + Value for the Nextflow `publishDir` mode parameter. + Available: symlink, rellink, link, copy, copyNoFollow, move. + - enable_conda: + type: boolean + description: | + Run the module with Conda using the software specified + via the `conda` directive + - singularity_pull_docker_container: + type: boolean + description: | + Instead of directly downloading Singularity images for use with Singularity, + force the workflow to pull and convert Docker containers instead. +input: + input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: list + description: | + List of input FastQ files to be concatenated. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: Merged fastq file + pattern: "*.{merged.fastq.gz}" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/tests/software/cat/fastq/main.nf b/tests/software/cat/fastq/main.nf new file mode 100644 index 00000000..24d203c1 --- /dev/null +++ b/tests/software/cat/fastq/main.nf @@ -0,0 +1,27 @@ +#!/usr/bin/env nextflow + +nextflow.enable.dsl = 2 + +include { CAT_FASTQ } from '../../../../software/cat/fastq/main.nf' addParams( options: [:] ) + +workflow test_cat_fastq_single_end { + + def input = [] + input = [ [ id:'test', single_end:true ], // meta map + [ file("${launchDir}/tests/data/fastq/rna/test_R1_val_1.fq.gz", checkIfExists: true), + file("${launchDir}/tests/data/fastq/rna/test_R1.fastq.gz", checkIfExists: true) ]] + + CAT_FASTQ ( input ) +} + +workflow test_cat_fastq_paired_end { + + def input = [] + input = [ [ id:'test', single_end:false ], // meta map + [ file("${launchDir}/tests/data/fastq/rna/test_R1_val_1.fq.gz", checkIfExists: true), + file("${launchDir}/tests/data/fastq/rna/test_R2_val_2.fq.gz", checkIfExists: true), + file("${launchDir}/tests/data/fastq/rna/test_R1.fastq.gz", checkIfExists: true), + file("${launchDir}/tests/data/fastq/rna/test_R2.fastq.gz", checkIfExists: true) ]] + + CAT_FASTQ ( input ) +} diff --git a/tests/software/cat/fastq/test.yml b/tests/software/cat/fastq/test.yml new file mode 100644 index 00000000..90e37084 --- /dev/null +++ b/tests/software/cat/fastq/test.yml @@ -0,0 +1,21 @@ +- name: cat fastq single-end + command: nextflow run ./tests/software/cat/fastq -entry test_cat_fastq_single_end -c tests/config/nextflow.config + tags: + - cat + - fastq + - cat_fastqc_single_end + files: + - path: output/merged_fastq/test.merged.fastq.gz + md5sum: 7f753b793e5b0872758b1574db84d767 + +- name: cat fastq fastqc_paired_end + command: nextflow run ./tests/software/cat/fastq -entry test_cat_fastq_paired_end -c tests/config/nextflow.config + tags: + - cat + - fastq + - cat_fastqc_paired_end + files: + - path: output/merged_fastq/test_1.merged.fastq.gz + md5sum: 7f753b793e5b0872758b1574db84d767 + - path: output/merged_fastq/test_2.merged.fastq.gz + md5sum: c71ff917e002b1e852916a021d52921d From d7a219e88d49285d01d524ae3b63ffefffb2ff77 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 12 Feb 2021 17:37:49 +0100 Subject: [PATCH 12/45] Fix test.yml --- tests/software/cat/fastq/test.yml | 6 +----- 1 file changed, 1 insertion(+), 5 deletions(-) diff --git a/tests/software/cat/fastq/test.yml b/tests/software/cat/fastq/test.yml index 90e37084..4c9a72fd 100644 --- a/tests/software/cat/fastq/test.yml +++ b/tests/software/cat/fastq/test.yml @@ -1,18 +1,14 @@ - name: cat fastq single-end command: nextflow run ./tests/software/cat/fastq -entry test_cat_fastq_single_end -c tests/config/nextflow.config tags: - - cat - - fastq - cat_fastqc_single_end files: - path: output/merged_fastq/test.merged.fastq.gz md5sum: 7f753b793e5b0872758b1574db84d767 -- name: cat fastq fastqc_paired_end +- name: cat fastq paired-end command: nextflow run ./tests/software/cat/fastq -entry test_cat_fastq_paired_end -c tests/config/nextflow.config tags: - - cat - - fastq - cat_fastqc_paired_end files: - path: output/merged_fastq/test_1.merged.fastq.gz From 8e23e1839dc01927a9af3d91ca558fb12ac5eee2 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 12 Feb 2021 17:56:51 +0100 Subject: [PATCH 13/45] Trying to fix tests --- software/cat/fastq/main.nf | 4 ++-- tests/software/cat/fastq/test.yml | 10 +++++++--- 2 files changed, 9 insertions(+), 5 deletions(-) diff --git a/software/cat/fastq/main.nf b/software/cat/fastq/main.nf index 44a06bb8..95db8d9a 100644 --- a/software/cat/fastq/main.nf +++ b/software/cat/fastq/main.nf @@ -1,5 +1,5 @@ // Import generic module functions -include { initOptions; saveFiles } from './functions' +include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] def options = initOptions(params.options) @@ -8,7 +8,7 @@ process CAT_FASTQ { tag "$meta.id" publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', publish_id:meta.id) } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } conda (params.enable_conda ? "conda-forge::sed=4.7=h1bed415_1000" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { diff --git a/tests/software/cat/fastq/test.yml b/tests/software/cat/fastq/test.yml index 4c9a72fd..a3122372 100644 --- a/tests/software/cat/fastq/test.yml +++ b/tests/software/cat/fastq/test.yml @@ -1,17 +1,21 @@ - name: cat fastq single-end command: nextflow run ./tests/software/cat/fastq -entry test_cat_fastq_single_end -c tests/config/nextflow.config tags: + - cat + - fastq - cat_fastqc_single_end files: - - path: output/merged_fastq/test.merged.fastq.gz + - path: output/cat/test.merged.fastq.gz md5sum: 7f753b793e5b0872758b1574db84d767 - name: cat fastq paired-end command: nextflow run ./tests/software/cat/fastq -entry test_cat_fastq_paired_end -c tests/config/nextflow.config tags: + - cat + - fastq - cat_fastqc_paired_end files: - - path: output/merged_fastq/test_1.merged.fastq.gz + - path: output/cat/test_1.merged.fastq.gz md5sum: 7f753b793e5b0872758b1574db84d767 - - path: output/merged_fastq/test_2.merged.fastq.gz + - path: output/cat/test_2.merged.fastq.gz md5sum: c71ff917e002b1e852916a021d52921d From 0688230863d3a45c9b107af40769ea0e064d69d2 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 12 Feb 2021 18:02:32 +0100 Subject: [PATCH 14/45] Revert since fixes to test were not working This reverts commit d7a219e88d49285d01d524ae3b63ffefffb2ff77. --- software/cat/fastq/main.nf | 4 ++-- tests/software/cat/fastq/test.yml | 8 ++++---- 2 files changed, 6 insertions(+), 6 deletions(-) diff --git a/software/cat/fastq/main.nf b/software/cat/fastq/main.nf index 95db8d9a..44a06bb8 100644 --- a/software/cat/fastq/main.nf +++ b/software/cat/fastq/main.nf @@ -1,5 +1,5 @@ // Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' +include { initOptions; saveFiles } from './functions' params.options = [:] def options = initOptions(params.options) @@ -8,7 +8,7 @@ process CAT_FASTQ { tag "$meta.id" publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', publish_id:meta.id) } conda (params.enable_conda ? "conda-forge::sed=4.7=h1bed415_1000" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { diff --git a/tests/software/cat/fastq/test.yml b/tests/software/cat/fastq/test.yml index a3122372..90e37084 100644 --- a/tests/software/cat/fastq/test.yml +++ b/tests/software/cat/fastq/test.yml @@ -5,17 +5,17 @@ - fastq - cat_fastqc_single_end files: - - path: output/cat/test.merged.fastq.gz + - path: output/merged_fastq/test.merged.fastq.gz md5sum: 7f753b793e5b0872758b1574db84d767 -- name: cat fastq paired-end +- name: cat fastq fastqc_paired_end command: nextflow run ./tests/software/cat/fastq -entry test_cat_fastq_paired_end -c tests/config/nextflow.config tags: - cat - fastq - cat_fastqc_paired_end files: - - path: output/cat/test_1.merged.fastq.gz + - path: output/merged_fastq/test_1.merged.fastq.gz md5sum: 7f753b793e5b0872758b1574db84d767 - - path: output/cat/test_2.merged.fastq.gz + - path: output/merged_fastq/test_2.merged.fastq.gz md5sum: c71ff917e002b1e852916a021d52921d From fafe068192f01293738d3f50db24aa58451c905d Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 12 Feb 2021 18:49:57 +0100 Subject: [PATCH 15/45] Fixing test tags --- tests/software/cat/fastq/test.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/software/cat/fastq/test.yml b/tests/software/cat/fastq/test.yml index 90e37084..b2235b6c 100644 --- a/tests/software/cat/fastq/test.yml +++ b/tests/software/cat/fastq/test.yml @@ -2,7 +2,7 @@ command: nextflow run ./tests/software/cat/fastq -entry test_cat_fastq_single_end -c tests/config/nextflow.config tags: - cat - - fastq + - cat_fastq - cat_fastqc_single_end files: - path: output/merged_fastq/test.merged.fastq.gz @@ -12,7 +12,7 @@ command: nextflow run ./tests/software/cat/fastq -entry test_cat_fastq_paired_end -c tests/config/nextflow.config tags: - cat - - fastq + - cat_fastq - cat_fastqc_paired_end files: - path: output/merged_fastq/test_1.merged.fastq.gz From e5a3e3a5b82d65bc3a7a53e7aca9c4107a0999e3 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 10:59:46 -0800 Subject: [PATCH 16/45] Add ivar consensus to filter.yml --- .github/filters.yml | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/.github/filters.yml b/.github/filters.yml index 83216113..bfc93d0d 100644 --- a/.github/filters.yml +++ b/.github/filters.yml @@ -116,6 +116,10 @@ gunzip: - software/gunzip/** - tests/software/gunzip/** +ivar_consensus: + - software/ivar/consensus/** + - tests/software/ivar/consensus/** + minimap2_align: - software/minimap2/align/** - tests/software/minimap2/align/** From 6b22bb9782803acb478ca5062f5c685759223f73 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 11:25:40 -0800 Subject: [PATCH 17/45] Add meta.yml --- software/ivar/consensus/meta.yml | 72 ++++++++++++++++++++++++++++++++ 1 file changed, 72 insertions(+) create mode 100644 software/ivar/consensus/meta.yml diff --git a/software/ivar/consensus/meta.yml b/software/ivar/consensus/meta.yml new file mode 100644 index 00000000..20416ba1 --- /dev/null +++ b/software/ivar/consensus/meta.yml @@ -0,0 +1,72 @@ +name: ivar_consensus +description: Generate a consensus sequence from a BAM file using iVar +keywords: + - amplicon sequencing + - consensus + - fasta +tools: + - ivar: + description: | + iVar - a computational package that contains functions broadly useful for viral amplicon-based sequencing. + homepage: https://github.com/andersen-lab/ivar + documentation: https://andersen-lab.github.io/ivar/html/manualpage.html +params: + - outdir: + type: string + description: | + The pipeline's output directory. By default, the module will + output files into `$params.outdir/` + - publish_dir_mode: + type: string + description: | + Value for the Nextflow `publishDir` mode parameter. + Available: symlink, rellink, link, copy, copyNoFollow, move. + - enable_conda: + type: boolean + description: | + Run the module with Conda using the software specified + via the `conda` directive + - singularity_pull_docker_container: + type: boolean + description: | + Instead of directly downloading Singularity images for use with Singularity, + force the workflow to pull and convert Docker containers instead. +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: A sorted (with samtools sort) and trimmed (with iVar trim) bam file + pattern: "*.bam" + - ref: + type: file + description: The reference sequence used for mapping and generating the BAM file + pattern: "*.fa" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: iVar generated consensus sequence + pattern: "*.fa" + - qual: + type: file + description: iVar generated quality file + pattern: "*.qual.txt" + - mpileup: + type: file + description: mpileup output from samtools mpileup [OPTIONAL] + pattern: "*.mpileup" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@andersgs" + - "@drpatelh" From a4bba006bda6c9aacfa89617dac20d7cd5cabdcb Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 11:26:55 -0800 Subject: [PATCH 18/45] Add reference fasta as input --- software/ivar/consensus/main.nf | 2 ++ 1 file changed, 2 insertions(+) diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf index a0288365..b30e8c8b 100644 --- a/software/ivar/consensus/main.nf +++ b/software/ivar/consensus/main.nf @@ -20,6 +20,7 @@ process IVAR_CONSENSUS { input: tuple val(meta), path(bam) + path(fasta) output: tuple val(meta), path("*.fa") , emit: fasta @@ -33,6 +34,7 @@ process IVAR_CONSENSUS { def save_mpileup = params.save_mpileup ? "tee ${prefix}.mpileup |" : "" """ samtools mpileup \\ + --fasta-ref $fasta -aa -A -d 0 -Q 0 \\ $options.args2 \\ $bam | \\ From 0a6e8400ace843f531560ca96a6b7db0466ddf4a Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 11:27:12 -0800 Subject: [PATCH 19/45] Add reference SC2 genome to test data --- tests/data/fasta/sarscov2/MN908947.3.fa | 500 ++++++++++++++++++++++++ 1 file changed, 500 insertions(+) create mode 100644 tests/data/fasta/sarscov2/MN908947.3.fa diff --git a/tests/data/fasta/sarscov2/MN908947.3.fa b/tests/data/fasta/sarscov2/MN908947.3.fa new file mode 100644 index 00000000..40729576 --- /dev/null +++ b/tests/data/fasta/sarscov2/MN908947.3.fa @@ -0,0 +1,500 @@ +>MN908947.3 +ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCT +GTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACT +CACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATC +TTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTT +CGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAAC +ACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGG +AGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGG +CTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAA +ACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACT +CGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGG +CGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGG +TGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGA +TCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACATAGCAGTGGTGTTACCCGTGA +ACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTGG +CCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTC +ATGCACTTTGTCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCG +TGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCA +GACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCAAA +TTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAA +GCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATG +CAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCA +GACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAGA +AGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGC +ATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGG +CTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTC +TTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAGG +TTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGA +AATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGA +GATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTGTGAA +AGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTAC +AAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCC +TCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCT +TGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATAACAATACTAGATGG +AATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTAC +TAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTG +GCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGA +AGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTAT +CTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTAA +GGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTC +TATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCA +CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCC +TCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTT +AACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGA +AGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGA +AATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATAC +CTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAGA +AGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGT +ACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGC +CTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACCAGTATCTGAATTACTTACACC +ACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGG +TGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGA +AGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGA +AGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGA +AGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGA +CGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATT +AGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTT +AAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGT +AAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTGC +AGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGC +TACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAA +ACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTTCTTAA +GAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTGG +TATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAA +TGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGA +AATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTAA +GCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAAT +CAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAA +CTTGTTACTTTATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAG +TGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATATATAGTGGGTGATGTTGTTCA +AGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAAT +GCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCA +GGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGC +CTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTC +TTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTG +TGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACA +AGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGC +GTCACTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTA +TGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGCC +AGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTC +TTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAA +AGATTGGTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGA +TAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCAC +CTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTAC +AACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACA +ACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTC +ACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTT +TGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCA +CACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAA +CAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGAGTTGAAGTTTAATCC +ACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGC +ACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAAT +GAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTG +TAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGG +CACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACA +AGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGC +TCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCA +GTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTTT +ACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAG +TTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAAT +TGACCCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAAT +TGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTATG +TGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTTC +AAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTA +TAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTG +GCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTG +TCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGA +CGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGT +GGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGT +AGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGGCCA +CACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGA +ATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAG +TGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTAC +AACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTT +CTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGC +ATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGA +GGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTG +GTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGGTGT +TTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAA +CTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCT +TAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTC +ATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATAT +TCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAG +CTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGT +ACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTA +TGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTG +TTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAAG +GTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTG +TGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGA +CTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGA +TAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGAC +TTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACAC +TAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATC +ATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACT +AGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTGA +TGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACT +AGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTAC +TTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGATGTTGT +TGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAA +CTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTAT +TGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGAT +ATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGC +TGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAA +TGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCA +GTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACC +TGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTAT +TGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATGC +TGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCC +ATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCAC +GATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAGT +TGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGC +TTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATA +TTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACAC +ACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAACACCTACCTTGAAGGTTC +TGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGATC +AGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAG +ATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTAC +ACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTAT +TGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGG +TGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACT +CTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTT +GACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACATATTCAGTGGATGGTTATGTT +CACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGCA +TTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTT +TAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAA +GTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAATAGATACTTAGCTCTTTATAA +TAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTG +TCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACC +ACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCC +ATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGG +TCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACAT +GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACA +GGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCT +TAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAGG +ACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTGC +TATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGG +TTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC +TGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGCA +AACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTA +CGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGA +CTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACAT +ACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGTGCTTCATTAAA +AGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAGA +TGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGT +GAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTT +AGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTT +ACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAA +GCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATAT +GGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATAC +TAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGTAGTGTTACT +AATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTAT +GAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTC +CATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT +GTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAAC +TGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTG +TTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGA +TTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAA +GAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCTTG +TATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTT +ACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGT +CCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGT +TTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGA +AGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCC +ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGA +TTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGA +CCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAAT +GTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAAT +GCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGC +AAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGT +TGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGC +ATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAG +TGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAG +GGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGAT +GTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTA +CAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAA +ATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACC +TTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAA +AGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCT +ACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTT +TGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCAC +TAATTGTGTTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACC +GGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACTGCCGTTG +CCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAAT +ACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGT +CTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCA +GTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACA +CCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGAT +AAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGAC +GAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTAC +CAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCAGCTGTTGCTAAACATGAC +TTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTACT +AAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGAC +ACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAG +GACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAA +CGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGT +ATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGT +GATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTG +TTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCAGAGTCACATGTTGACACTGAC +TTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTA +AAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAAC +TGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTG +TTCCCACCTACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTT +GTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAAC +TTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATG +CACGCTGCTTCTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCA +CTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTTAACAAAGACTTCTAT +GACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTC +TTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTA +CCAACAATGTGTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTT +GATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAA +TCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAGT +TATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACT +CAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTC +TCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCC +GCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAAC +ATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCT +AAATGTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGC +AAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCT +CAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAACC +TCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTC +ACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTC +CGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGAC +TTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGAC +GATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAG +AACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGG +ACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTT +AAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCC +GGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTG +TCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTC +TTTCATTTGTACTTACAATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTA +GACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTT +TATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGC +AATTCACAGACTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAA +TGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTAT +GTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATG +AGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAA +GTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCA +ATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAA +AGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTCT +TATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAA +GTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACT +AAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCT +GTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACA +TCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGA +ATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAAT +TATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACCACCTGGTACTGGTAAG +AGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCT +TGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGAT +AAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTG +AATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCA +GATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAAT +GCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCA +CGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTT +ATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATT +GTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCA +GCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATT +AACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAA +GCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTA +CCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAA +ACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCA +AAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACA +AGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAATGTAACAGGACTC +TTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTC +AGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAG +GACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAAT +GGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCATGGATT +GGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTA +CAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACA +CCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAA +CACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTA +CAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCA +CATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGT +TGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGG +CATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAACAATGG +GGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGCA +CATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTT +AAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCG +GCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCA +GTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAA +TGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTC +TATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGC +AATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGTGCTATCT +AACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCAC +ACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTAC +TCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCA +CTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCAT +GCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGC +TTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAG +AGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGT +GAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTA +GAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAG +CGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCT +GCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGT +GTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCACTCACT +GTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGT +GTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCT +AGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAG +AAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTA +CAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAA +TTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTT +AGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAA +TCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATA +ACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGAT +GATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTG +ACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA +TTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTT +TACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCA +ACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTA +AACACATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCT +GATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTGCCTACGGGTACGCTG +CTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGAT +TGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCT +AAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGT +GGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACAT +TCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTT +ACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAA +CCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACA +AATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCCCTTAAATTA +AGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTT +CTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTT +CTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAG +TCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCAC +ACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGGA +CTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGAC +CAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGC +TTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAA +GACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATT +TCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGAT +GGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCA +GCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTCAAAAATCTTAGGGAATTTGT +GTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAGT +GCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTAT +TAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGA +TTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAG +GACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACT +TGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTA +TCAAACTTCTAACTTTAGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTAC +AAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGCATCTGTTTATGCTTG +GAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCATC +ATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTAC +TAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGG +GCAAACTGGAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTTACAGGCTGCGT +TATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGTA +TAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTA +TCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACA +ATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACT +TTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTT +GGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTAC +TGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTAC +TGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTACACCATGTTCTTTTGG +TGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATCA +GGATGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTG +GCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGC +TGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAG +TTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCAT +TGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGC +CATACCCACAAATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAA +GACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTT +GTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTGA +ACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACC +AATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAG +CAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTT +CATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACA +AAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATA +CACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGC +ATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTATTGGAGTTACACA +GAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCAA +AATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAA +CCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAAT +TTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAAT +TGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAAT +TAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGT +ACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCC +TCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAA +GAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGG +TGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACA +AATCATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGT +CAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGA +TAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAA +TGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTT +AAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCC +ATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATTAT +GCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTG +CTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACAC +ATAAACGAACTTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAG +CAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTCGCGCTACTGCAACGATACCG +ATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTTT +CAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGT +GTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTC +GTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGCAG +AGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAA +AACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTAT +TGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACA +AGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAAAAATGGGAATCTGGA +GTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCA +ACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATT +GTTGATGAGCCTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTT +AATCCAGTAATGGAACCAATTTATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAA +GCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTA +ATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCC +ATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTA +AAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCCTGAT +CTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAG +CCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAAT +GGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATG +CCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTCCTCTGGCTGTTATGGCCAG +TAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGAA +TTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTT +TCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTC +TCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAA +TCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTG +ACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACA +AATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACA +GGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGC +TTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGAG +ATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATA +AACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGAT +GAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTG +ATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTA +CTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTA +GCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGAC +GGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTGTTCATCAGA +CAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTT +ATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACT +TCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTT +GGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATGAAAT +TTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTAC +AGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATT +CTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGG +ATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCCT +GTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTT +CGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAA +CGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTAC +GTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCG +ATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCT +CACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACAC +CAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGG +TGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTGG +GCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGA +GGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGC +AATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAG +CAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAA +TTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGA +TGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGG +TAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAA +GAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAG +ACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAAC +TGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGG +AATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGC +CATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCA +TATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGC +TGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCC +TGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTC +AACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGC +TTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGC +ACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTA +GGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGT +ACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAAT +TTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAATGACAAAAAAAAAA +AAAAAAAAAAAAAAAAAAAAAAA From 8b3c16a518f2817f183452a9cbb5999a16f879b8 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 11:29:16 -0800 Subject: [PATCH 20/45] Add ref FASTA to test main.nf --- tests/software/ivar/consensus/main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/software/ivar/consensus/main.nf b/tests/software/ivar/consensus/main.nf index 5fdc851e..3436b417 100644 --- a/tests/software/ivar/consensus/main.nf +++ b/tests/software/ivar/consensus/main.nf @@ -5,9 +5,9 @@ nextflow.enable.dsl = 2 include { IVAR_CONSENSUS } from '../../../../software/ivar/consensus/main.nf' addParams([:]) workflow test_ivar_consensus { - + def ref = file("${launchDir}/tests/data/fasta/sarscov2/MN908947.3.fa", checkIfExists: true) def input = [] input = [ [ id:'test'], file("${launchDir}/tests/data/bam/test-sc2-artic-v3-sorted-trimmed.bam", checkIfExists: true) ] - IVAR_CONSENSUS ( input ) + IVAR_CONSENSUS ( input, ref ) } From bfc13d337969cc42da11bc3bf44d2244a804d582 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 11:50:33 -0800 Subject: [PATCH 21/45] Add options to make samtools more flexible --- software/ivar/consensus/main.nf | 15 ++++++++++++--- 1 file changed, 12 insertions(+), 3 deletions(-) diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf index b30e8c8b..eba6276d 100644 --- a/software/ivar/consensus/main.nf +++ b/software/ivar/consensus/main.nf @@ -31,11 +31,20 @@ process IVAR_CONSENSUS { script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def save_mpileup = params.save_mpileup ? "tee ${prefix}.mpileup |" : "" + def save_mpileup = options.save_mpileup ? "tee ${prefix}.mpileup |" : "" + def min_bq = options.min_bq ? "-Q ${options.min_bq}" : "-Q 0" + def max_depth = options.max_depth ? "-d ${options.max_depth}" : "-d 0" + def count_orphans = options.count_orphans ? "-A" : "" + def disable_baq = options.disable_baq ? "-B" : "" + def output_all_bases = options.output_all_bases ? "-aa" : "" """ samtools mpileup \\ - --fasta-ref $fasta - -aa -A -d 0 -Q 0 \\ + --fasta-ref $fasta \\ + $output_all_bases \\ + $count_orphans \\ + $disable_baq \\ + $max_depth \\ + $min_bq \\ $options.args2 \\ $bam | \\ $save_mpileup \\ From 487ec7b7939e56805b4a16006571fdd59136d9b4 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 14:42:42 -0800 Subject: [PATCH 22/45] Fix test.yml --- tests/software/ivar/consensus/test.yml | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/tests/software/ivar/consensus/test.yml b/tests/software/ivar/consensus/test.yml index 4e799629..27b9d959 100644 --- a/tests/software/ivar/consensus/test.yml +++ b/tests/software/ivar/consensus/test.yml @@ -1,5 +1,5 @@ - name: ivar consensus - command: nextflow run ./tests/software/ivar/consensus -profile docker -entry test_ivar_consensus -c tests/config/nextflow.config + command: nextflow run ./tests/software/ivar/consensus -entry test_ivar_consensus -c tests/config/nextflow.config tags: - ivar - ivar_consensus @@ -7,6 +7,6 @@ - path: output/ivar/test.fa md5sum: d791e777b669bd40f6074b5566722a47 - path: output/ivar/test.qual.txt - md5sum: 723126475ec74adfd876e2b47ffe35a8 + md5sum: 2546e23f835c9f73edef4323effa772f - path: output/ivar/test.mpileup - md5sum: d1c0ef3978bec1c09c549f884ed5b76a + md5sum: 086996c20a3c55a5291f1f018a0ec1bd From ffc849761d31dffdb047de74fef51826d6161577 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 14:43:19 -0800 Subject: [PATCH 23/45] Edit test to output mpileup and passing args to samtools --- tests/software/ivar/consensus/main.nf | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/tests/software/ivar/consensus/main.nf b/tests/software/ivar/consensus/main.nf index 3436b417..271d4eb2 100644 --- a/tests/software/ivar/consensus/main.nf +++ b/tests/software/ivar/consensus/main.nf @@ -2,7 +2,10 @@ nextflow.enable.dsl = 2 -include { IVAR_CONSENSUS } from '../../../../software/ivar/consensus/main.nf' addParams([:]) +params.save_mpileup = true +include { IVAR_CONSENSUS } from '../../../../software/ivar/consensus/main.nf' addParams([ + options: [args2: '-aa -A -d 0 -Q 0'] +]) workflow test_ivar_consensus { def ref = file("${launchDir}/tests/data/fasta/sarscov2/MN908947.3.fa", checkIfExists: true) From b08bf1ea5c2bd98ea035dfe2093a5ed4ad8d20c3 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 14:43:50 -0800 Subject: [PATCH 24/45] Remove ref to conda build and additional params --- software/ivar/consensus/main.nf | 16 ++++------------ 1 file changed, 4 insertions(+), 12 deletions(-) diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf index eba6276d..9850a0ef 100644 --- a/software/ivar/consensus/main.nf +++ b/software/ivar/consensus/main.nf @@ -11,7 +11,7 @@ process IVAR_CONSENSUS { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } - conda (params.enable_conda ? "bioconda::ivar=1.3.1=h089eab3_0" : null) + conda (params.enable_conda ? "bioconda::ivar=1.3.1" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/ivar:1.3.1--h089eab3_0" } else { @@ -31,20 +31,12 @@ process IVAR_CONSENSUS { script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def save_mpileup = options.save_mpileup ? "tee ${prefix}.mpileup |" : "" - def min_bq = options.min_bq ? "-Q ${options.min_bq}" : "-Q 0" - def max_depth = options.max_depth ? "-d ${options.max_depth}" : "-d 0" - def count_orphans = options.count_orphans ? "-A" : "" - def disable_baq = options.disable_baq ? "-B" : "" - def output_all_bases = options.output_all_bases ? "-aa" : "" + def save_mpileup = params.save_mpileup ? "tee ${prefix}.mpileup |" : "" + """ samtools mpileup \\ --fasta-ref $fasta \\ - $output_all_bases \\ - $count_orphans \\ - $disable_baq \\ - $max_depth \\ - $min_bq \\ + -aa \\ $options.args2 \\ $bam | \\ $save_mpileup \\ From cb19c7b056cda9473438648cfe0d18111608a56b Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 14:46:13 -0800 Subject: [PATCH 25/45] Fix trailing whitespace --- software/ivar/consensus/meta.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/software/ivar/consensus/meta.yml b/software/ivar/consensus/meta.yml index 20416ba1..281b39d9 100644 --- a/software/ivar/consensus/meta.yml +++ b/software/ivar/consensus/meta.yml @@ -7,7 +7,7 @@ keywords: tools: - ivar: description: | - iVar - a computational package that contains functions broadly useful for viral amplicon-based sequencing. + iVar - a computational package that contains functions broadly useful for viral amplicon-based sequencing. homepage: https://github.com/andersen-lab/ivar documentation: https://andersen-lab.github.io/ivar/html/manualpage.html params: @@ -59,7 +59,7 @@ output: type: file description: iVar generated quality file pattern: "*.qual.txt" - - mpileup: + - mpileup: type: file description: mpileup output from samtools mpileup [OPTIONAL] pattern: "*.mpileup" From bc5e073cdb849d1a47421d8d2e5fd04f61bad8db Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Fri, 12 Feb 2021 22:57:06 +0000 Subject: [PATCH 26/45] Update software/ivar/consensus/main.nf --- software/ivar/consensus/main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf index 9850a0ef..e9061f70 100644 --- a/software/ivar/consensus/main.nf +++ b/software/ivar/consensus/main.nf @@ -41,8 +41,8 @@ process IVAR_CONSENSUS { $bam | \\ $save_mpileup \\ ivar consensus \\ - $options.args \\ - -p $prefix + $options.args \\ + -p $prefix ivar version | head -n1 2>&1 | sed 's/^.*iVar version //g' > ${software}.version.txt """ From 9e4539473ee58e9b5fa7478f7ebae129748f002d Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Fri, 12 Feb 2021 23:01:53 +0000 Subject: [PATCH 27/45] Update software/ivar/consensus/main.nf --- software/ivar/consensus/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf index e9061f70..a01a5ed7 100644 --- a/software/ivar/consensus/main.nf +++ b/software/ivar/consensus/main.nf @@ -20,7 +20,7 @@ process IVAR_CONSENSUS { input: tuple val(meta), path(bam) - path(fasta) + path fasta output: tuple val(meta), path("*.fa") , emit: fasta From 0580fd8ded2fa053a8252c557c5cdc97395e962b Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Fri, 12 Feb 2021 23:02:04 +0000 Subject: [PATCH 28/45] Update software/ivar/consensus/main.nf --- software/ivar/consensus/main.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/software/ivar/consensus/main.nf b/software/ivar/consensus/main.nf index a01a5ed7..cf728fdd 100644 --- a/software/ivar/consensus/main.nf +++ b/software/ivar/consensus/main.nf @@ -32,7 +32,6 @@ process IVAR_CONSENSUS { def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def save_mpileup = params.save_mpileup ? "tee ${prefix}.mpileup |" : "" - """ samtools mpileup \\ --fasta-ref $fasta \\ From f8916060f87999994641b5f60f45ee843342a7f0 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Fri, 12 Feb 2021 23:02:19 +0000 Subject: [PATCH 29/45] Update tests/software/ivar/consensus/main.nf --- tests/software/ivar/consensus/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/software/ivar/consensus/main.nf b/tests/software/ivar/consensus/main.nf index 271d4eb2..fe82867b 100644 --- a/tests/software/ivar/consensus/main.nf +++ b/tests/software/ivar/consensus/main.nf @@ -12,5 +12,5 @@ workflow test_ivar_consensus { def input = [] input = [ [ id:'test'], file("${launchDir}/tests/data/bam/test-sc2-artic-v3-sorted-trimmed.bam", checkIfExists: true) ] - IVAR_CONSENSUS ( input, ref ) + IVAR_CONSENSUS ( input, ref ) } From 96d3b0a18b282e5a15600902a01c0273c191bd8a Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Fri, 12 Feb 2021 23:02:25 +0000 Subject: [PATCH 30/45] Update tests/software/ivar/consensus/main.nf --- tests/software/ivar/consensus/main.nf | 4 +--- 1 file changed, 1 insertion(+), 3 deletions(-) diff --git a/tests/software/ivar/consensus/main.nf b/tests/software/ivar/consensus/main.nf index fe82867b..0cc56015 100644 --- a/tests/software/ivar/consensus/main.nf +++ b/tests/software/ivar/consensus/main.nf @@ -3,9 +3,7 @@ nextflow.enable.dsl = 2 params.save_mpileup = true -include { IVAR_CONSENSUS } from '../../../../software/ivar/consensus/main.nf' addParams([ - options: [args2: '-aa -A -d 0 -Q 0'] -]) +include { IVAR_CONSENSUS } from '../../../../software/ivar/consensus/main.nf' addParams( [ options: [args2: '-aa -A -d 0 -Q 0'] ] ) workflow test_ivar_consensus { def ref = file("${launchDir}/tests/data/fasta/sarscov2/MN908947.3.fa", checkIfExists: true) From 53136987053e22197dbcde0ba27995f08904bf58 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Sat, 13 Feb 2021 00:08:57 +0100 Subject: [PATCH 31/45] Add bcftools merge module --- .github/filters.yml | 4 ++ software/bcftools/merge/functions.nf | 60 ++++++++++++++++++++++ software/bcftools/merge/main.nf | 37 ++++++++++++++ software/bcftools/merge/meta.yml | 67 +++++++++++++++++++++++++ tests/data/vcf/test_a.merge.vcf.gz | Bin 0 -> 763 bytes tests/data/vcf/test_a.merge.vcf.gz.tbi | Bin 0 -> 141 bytes tests/data/vcf/test_b.merge.vcf.gz | Bin 0 -> 769 bytes tests/data/vcf/test_b.merge.vcf.gz.tbi | Bin 0 -> 141 bytes tests/software/bcftools/merge/main.nf | 18 +++++++ tests/software/bcftools/merge/test.yml | 8 +++ 10 files changed, 194 insertions(+) create mode 100644 software/bcftools/merge/functions.nf create mode 100644 software/bcftools/merge/main.nf create mode 100644 software/bcftools/merge/meta.yml create mode 100644 tests/data/vcf/test_a.merge.vcf.gz create mode 100644 tests/data/vcf/test_a.merge.vcf.gz.tbi create mode 100644 tests/data/vcf/test_b.merge.vcf.gz create mode 100644 tests/data/vcf/test_b.merge.vcf.gz.tbi create mode 100644 tests/software/bcftools/merge/main.nf create mode 100644 tests/software/bcftools/merge/test.yml diff --git a/.github/filters.yml b/.github/filters.yml index f63a162d..a88c933c 100644 --- a/.github/filters.yml +++ b/.github/filters.yml @@ -18,6 +18,10 @@ bcftools_isec: - software/bcftools/isec/** - tests/software/bcftools/isec/** +bcftools_merge: + - software/bcftools/merge/** + - tests/software/bcftools/merge/** + bcftools_stats: - software/bcftools/stats/** - tests/software/bcftools/stats/** diff --git a/software/bcftools/merge/functions.nf b/software/bcftools/merge/functions.nf new file mode 100644 index 00000000..6f3b4b29 --- /dev/null +++ b/software/bcftools/merge/functions.nf @@ -0,0 +1,60 @@ + +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", '') } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/software/bcftools/merge/main.nf b/software/bcftools/merge/main.nf new file mode 100644 index 00000000..e345c475 --- /dev/null +++ b/software/bcftools/merge/main.nf @@ -0,0 +1,37 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +process BCFTOOLS_MERGE { + tag "$meta.id" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } + + conda (params.enable_conda ? "bioconda::bcftools=1.11=h7c999a4_0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/bcftools:1.11--h7c999a4_0" + } else { + container "quay.io/biocontainers/bcftools:1.11--h7c999a4_0" + } + + input: + tuple val(meta), path(vcfs), path(tbis) + + output: + tuple val(meta), path("*.gz"), emit: vcf + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + bcftools merge -Oz\\ + --output ${prefix}.vcf.gz \\ + $options.args \\ + *.vcf.gz + echo \$(bcftools --version 2>&1) | sed 's/^.*bcftools //; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/software/bcftools/merge/meta.yml b/software/bcftools/merge/meta.yml new file mode 100644 index 00000000..dff0523b --- /dev/null +++ b/software/bcftools/merge/meta.yml @@ -0,0 +1,67 @@ +name: bcftools_merge +description: Merge VCF files +keywords: + - variant calling + - merge + - VCF +tools: + - isec: + description: | + Merge VCF files. + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: http://www.htslib.org/doc/bcftools.html + doi: 10.1093/bioinformatics/btp352 +params: + - outdir: + type: string + description: | + The pipeline's output directory. By default, the module will + output files into `$params.outdir/` + - publish_dir_mode: + type: string + description: | + Value for the Nextflow `publishDir` mode parameter. + Available: symlink, rellink, link, copy, copyNoFollow, move. + - enable_conda: + type: boolean + description: | + Run the module with Conda using the software specified + via the `conda` directive + - singularity_pull_docker_container: + type: boolean + description: | + Instead of directly downloading Singularity images for use with Singularity, + force the workflow to pull and convert Docker containers instead. +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcfs: + type: files + description: | + List containing 2 or more vcf files + e.g. [ 'file1.vcf', 'file2.vcf' ] + - tbis: + type: files + description: | + List containing the tbi index files corresponding to the vcfs input files + e.g. [ 'file1.vcf.tbi', 'file2.vcf.tbi' ] +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF merged output file + pattern: "*.{vcf.gz}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/tests/data/vcf/test_a.merge.vcf.gz b/tests/data/vcf/test_a.merge.vcf.gz new file mode 100644 index 0000000000000000000000000000000000000000..7ab659d78f2160ed965fd0ef136a501a1d1047b8 GIT binary patch literal 763 zcmVNEnvctRr>;HdFK00xu!og0*h zBfz~3puwkv$1u#Z3^R!78ZH|`inoL<8>(OSsyCC;RYJvpGA@_P#^WyMLM~fF1$11I zH?^yH>@)E+VVp$qm&YAW6aEYfviTx*=?NEWxWbGuO$`-fPYOmfh*N%?ml4YTVY1ZM zcV$@L)&(!b`cH!x5}I={`NllERJ%@1Nilq4yL`K~p4wk>4JR+AlDU?3UDNiD$M;c~~ zWUKVqSy|UwN@&Dm$Fy(Ew$U+BO>O>@+Im-wZoV3(Xg16>bjop7%zw}mWz29BcrWvi~J7>InyKbpbLYZ;e_5FM)i1?VR|p7?)TPpYl9ib zii_77aa`42`nRgRt`E0&Nx8adN>U;UZ$~*yD2xv0%ub8V9ba^LjW1q|GUpHfm%5)u-ak|cPUP6bFEF;7WgG;CGWii%J)+Lj}5 zD_h7#)MrlPL%SOSd~6{Hn6K8);0=|P4v|Td^ZCayY3AZD8n#~jmll8dup}|nexu1E bzD5Ry_x#Hq9b;o)kVmsnnt>T?KZpPT-=Zl6 literal 0 HcmV?d00001 diff --git a/tests/data/vcf/test_b.merge.vcf.gz b/tests/data/vcf/test_b.merge.vcf.gz new file mode 100644 index 0000000000000000000000000000000000000000..751f5a66d4cf4df0039d538b18e2dc58fe1fd97a GIT binary patch literal 769 zcmV+c1OEIUiwFb&00000{{{d;LjnNg0;QB)bD}U5hOg6Kki5ySadTyzi5fa1PDpI!@?1%TprkUv6(_weVH5IT-cE z_P2p&`}cDnjkf8M<~BzD>xS9`CTK`=#PSf9kxgi#zu*Uwv$#Nrj^bbT#v>4oGgxFno%R7~Y`M{`aQB#Ahzs|eM2zgX() zx6-d)>s;jW{KrA`Da(Xh{9vA5@=Yo?oR){EQ@qI~lo=%}=+TXcK14CB{Qc73OEMzP z#4wDXXc9+Rwkk1$p?^OsFwa;&_UyxjOPXXa5bJe={IKG5^2l5kG%xf)#zY(zdW;C? 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"${meta.id}${options.suffix}" : "${meta.id}" """ - bcftools merge -Oz\\ + bcftools merge -Oz \\ --output ${prefix}.vcf.gz \\ $options.args \\ *.vcf.gz From 6dca7799e6a06d6c73b98617619397cb21683e46 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 16:02:10 -0800 Subject: [PATCH 35/45] Add ivar trim --- .github/filters.yml | 4 ++ software/ivar/trim/functions.nf | 59 ++++++++++++++++++++++++++++ software/ivar/trim/main.nf | 43 ++++++++++++++++++++ tests/data/bed/test-sc2-artic-v3.bed | 4 ++ tests/software/ivar/trim/main.nf | 19 +++++++++ tests/software/ivar/trim/test.yml | 8 ++++ 6 files changed, 137 insertions(+) create mode 100644 software/ivar/trim/functions.nf create mode 100644 software/ivar/trim/main.nf create mode 100644 tests/data/bed/test-sc2-artic-v3.bed create mode 100644 tests/software/ivar/trim/main.nf create mode 100644 tests/software/ivar/trim/test.yml diff --git a/.github/filters.yml b/.github/filters.yml index f074dc9d..5a1ed42e 100644 --- a/.github/filters.yml +++ b/.github/filters.yml @@ -132,6 +132,10 @@ ivar_consensus: - software/ivar/consensus/** - tests/software/ivar/consensus/** +ivar_trim: + - software/ivar/trim/** + - tests/software/ivar/trim/** + minimap2_align: - software/minimap2/align/** - tests/software/minimap2/align/** diff --git a/software/ivar/trim/functions.nf b/software/ivar/trim/functions.nf new file mode 100644 index 00000000..d25eea86 --- /dev/null +++ b/software/ivar/trim/functions.nf @@ -0,0 +1,59 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/software/ivar/trim/main.nf b/software/ivar/trim/main.nf new file mode 100644 index 00000000..e6ac8888 --- /dev/null +++ b/software/ivar/trim/main.nf @@ -0,0 +1,43 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +process IVAR_TRIM { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } + + conda (params.enable_conda ? "bioconda::ivar=1.3.1" : null) + + + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/ivar:1.3.1--h089eab3_0" + } else { + container "quay.io/biocontainers/ivar:1.3.1--h089eab3_0" + } + + input: + tuple val(meta), path(bam) + path bed + + output: + tuple val(meta), path("*.bam"), emit: bam + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + ivar trim \\ + $options.args \\ + -i $bam \\ + -b $bed \\ + -p $prefix + + ivar version | head -n1 2>&1 | sed 's/^.*iVar version //g' > ${software}.version.txt + """ +} diff --git a/tests/data/bed/test-sc2-artic-v3.bed b/tests/data/bed/test-sc2-artic-v3.bed new file mode 100644 index 00000000..fbd2c968 --- /dev/null +++ b/tests/data/bed/test-sc2-artic-v3.bed @@ -0,0 +1,4 @@ +MN908947.3 1242 1264 nCoV-2019_5_LEFT 1 + +MN908947.3 1623 1651 nCoV-2019_5_RIGHT 1 - +MN908947.3 1573 1595 nCoV-2019_6_LEFT 2 + +MN908947.3 1942 1964 nCoV-2019_6_RIGHT 2 - \ No newline at end of file diff --git a/tests/software/ivar/trim/main.nf b/tests/software/ivar/trim/main.nf new file mode 100644 index 00000000..885da9ad --- /dev/null +++ b/tests/software/ivar/trim/main.nf @@ -0,0 +1,19 @@ +#!/usr/bin/env nextflow + +nextflow.enable.dsl = 2 +params.publish_dir_mode = 'link' +params.enable_conda = false +params.run_id = "test-run" + +include { IVAR_TRIM } from '../../../../software/ivar/trim/main.nf' addParams([:]) + +workflow test_ivar_trim { + bed_file = file("${launchDir}/tests/data/bed/test-sc2-artic-v3.bed", checkIfExists: true) + + def input = [] + input = [ [ id:'test'], + file("${launchDir}/tests/data/bam/test-sc2-artic-v3.bam", checkIfExists: true) ] + + main: + IVAR_TRIM ( input, bed_file ) +} diff --git a/tests/software/ivar/trim/test.yml b/tests/software/ivar/trim/test.yml new file mode 100644 index 00000000..75f1138c --- /dev/null +++ b/tests/software/ivar/trim/test.yml @@ -0,0 +1,8 @@ +- name: Run ivar trim test workflow + command: nextflow run ./tests/software/ivar/trim -profile docker -entry test_ivar_trim -c tests/config/nextflow.config + tags: + - ivar + - ivar_consensus + files: + - path: output/ivar/test.bam + md5sum: 09fc287d484f3a56d6868e5a3deace78 \ No newline at end of file From 7c9766962d26d1e1d47f2ab5e003e8cd969dfc59 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 16:02:48 -0800 Subject: [PATCH 36/45] Add ivar trim meta.yml --- software/ivar/trim/meta.yml | 72 +++++++++++++++++++++++++++++++++++++ 1 file changed, 72 insertions(+) create mode 100644 software/ivar/trim/meta.yml diff --git a/software/ivar/trim/meta.yml b/software/ivar/trim/meta.yml new file mode 100644 index 00000000..e574beed --- /dev/null +++ b/software/ivar/trim/meta.yml @@ -0,0 +1,72 @@ +name: ivar_trim +description: Trim primer sequences rom a BAM file with iVar +keywords: + - amplicon sequencing + - trimming + - fasta +tools: + - ivar: + description: | + iVar - a computational package that contains functions broadly useful for viral amplicon-based sequencing. + homepage: https://github.com/andersen-lab/ivar + documentation: https://andersen-lab.github.io/ivar/html/manualpage.html +params: + - outdir: + type: string + description: | + The pipeline's output directory. By default, the module will + output files into `$params.outdir/` + - publish_dir_mode: + type: string + description: | + Value for the Nextflow `publishDir` mode parameter. + Available: symlink, rellink, link, copy, copyNoFollow, move. + - enable_conda: + type: boolean + description: | + Run the module with Conda using the software specified + via the `conda` directive + - singularity_pull_docker_container: + type: boolean + description: | + Instead of directly downloading Singularity images for use with Singularity, + force the workflow to pull and convert Docker containers instead. +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: A sorted (with samtools sort) bam file + pattern: "*.bam" + - bed: + type: file + description: BED file with primer labels and positions + pattern: "*.bed" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: iVar generated consensus sequence + pattern: "*.fa" + - qual: + type: file + description: iVar generated quality file + pattern: "*.qual.txt" + - mpileup: + type: file + description: mpileup output from samtools mpileup [OPTIONAL] + pattern: "*.mpileup" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@andersgs" + - "@drpatelh" From ed74d366bf56b0f2b0a698c0cf61fabc450a703a Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 16:03:07 -0800 Subject: [PATCH 37/45] Clean up ivar trim main.nf --- tests/software/ivar/trim/main.nf | 3 --- 1 file changed, 3 deletions(-) diff --git a/tests/software/ivar/trim/main.nf b/tests/software/ivar/trim/main.nf index 885da9ad..b77d104f 100644 --- a/tests/software/ivar/trim/main.nf +++ b/tests/software/ivar/trim/main.nf @@ -1,9 +1,6 @@ #!/usr/bin/env nextflow nextflow.enable.dsl = 2 -params.publish_dir_mode = 'link' -params.enable_conda = false -params.run_id = "test-run" include { IVAR_TRIM } from '../../../../software/ivar/trim/main.nf' addParams([:]) From 47670a12ad139dd7996982fb831ba9fde9f59310 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 16:03:18 -0800 Subject: [PATCH 38/45] Fix ivar trim test.yml --- tests/software/ivar/trim/test.yml | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/tests/software/ivar/trim/test.yml b/tests/software/ivar/trim/test.yml index 75f1138c..ae1207e5 100644 --- a/tests/software/ivar/trim/test.yml +++ b/tests/software/ivar/trim/test.yml @@ -1,8 +1,8 @@ -- name: Run ivar trim test workflow - command: nextflow run ./tests/software/ivar/trim -profile docker -entry test_ivar_trim -c tests/config/nextflow.config +- name: ivar trim + command: nextflow run ./tests/software/ivar/trim -entry test_ivar_trim -c tests/config/nextflow.config tags: - ivar - - ivar_consensus + - ivar_trim files: - path: output/ivar/test.bam md5sum: 09fc287d484f3a56d6868e5a3deace78 \ No newline at end of file From bbdca4fa76e8888642c5d5ed5b6845d50c85cae1 Mon Sep 17 00:00:00 2001 From: Anders Goncalves da Silva Date: Fri, 12 Feb 2021 16:14:46 -0800 Subject: [PATCH 39/45] Fix output files --- software/ivar/trim/meta.yml | 12 ++---------- 1 file changed, 2 insertions(+), 10 deletions(-) diff --git a/software/ivar/trim/meta.yml b/software/ivar/trim/meta.yml index e574beed..f35627e9 100644 --- a/software/ivar/trim/meta.yml +++ b/software/ivar/trim/meta.yml @@ -51,18 +51,10 @@ output: description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - fasta: + - bam: type: file - description: iVar generated consensus sequence + description: iVar generated trimmed bam file (unsorted) pattern: "*.fa" - - qual: - type: file - description: iVar generated quality file - pattern: "*.qual.txt" - - mpileup: - type: file - description: mpileup output from samtools mpileup [OPTIONAL] - pattern: "*.mpileup" - version: type: file description: File containing software version From cdd9be0a2d5b941ae02458d6fdf44adb433fc5a6 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Sat, 13 Feb 2021 08:16:44 +0000 Subject: [PATCH 40/45] Update tests/software/ivar/trim/main.nf --- tests/software/ivar/trim/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/software/ivar/trim/main.nf b/tests/software/ivar/trim/main.nf index b77d104f..b18358cd 100644 --- a/tests/software/ivar/trim/main.nf +++ b/tests/software/ivar/trim/main.nf @@ -12,5 +12,5 @@ workflow test_ivar_trim { file("${launchDir}/tests/data/bam/test-sc2-artic-v3.bam", checkIfExists: true) ] main: - IVAR_TRIM ( input, bed_file ) + IVAR_TRIM ( input, bed_file ) } From 25f3d46f683169878affbcec86e96dac1471138e Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Sat, 13 Feb 2021 08:17:03 +0000 Subject: [PATCH 41/45] Update software/ivar/trim/meta.yml --- software/ivar/trim/meta.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/software/ivar/trim/meta.yml b/software/ivar/trim/meta.yml index f35627e9..4b72d968 100644 --- a/software/ivar/trim/meta.yml +++ b/software/ivar/trim/meta.yml @@ -54,7 +54,7 @@ output: - bam: type: file description: iVar generated trimmed bam file (unsorted) - pattern: "*.fa" + pattern: "*.bam" - version: type: file description: File containing software version From 9d6f29fc61631bcffdc1794bedc67d3573415f71 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Sat, 13 Feb 2021 08:17:15 +0000 Subject: [PATCH 42/45] Update tests/software/ivar/trim/main.nf --- tests/software/ivar/trim/main.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/tests/software/ivar/trim/main.nf b/tests/software/ivar/trim/main.nf index b18358cd..91f1fcdb 100644 --- a/tests/software/ivar/trim/main.nf +++ b/tests/software/ivar/trim/main.nf @@ -11,6 +11,5 @@ workflow test_ivar_trim { input = [ [ id:'test'], file("${launchDir}/tests/data/bam/test-sc2-artic-v3.bam", checkIfExists: true) ] - main: IVAR_TRIM ( input, bed_file ) } From f51f8c640015660ba00e19a820ea7d613bd2c083 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Sat, 13 Feb 2021 08:18:32 +0000 Subject: [PATCH 43/45] Update tests/software/ivar/trim/main.nf --- tests/software/ivar/trim/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/software/ivar/trim/main.nf b/tests/software/ivar/trim/main.nf index 91f1fcdb..94fc1e10 100644 --- a/tests/software/ivar/trim/main.nf +++ b/tests/software/ivar/trim/main.nf @@ -11,5 +11,5 @@ workflow test_ivar_trim { input = [ [ id:'test'], file("${launchDir}/tests/data/bam/test-sc2-artic-v3.bam", checkIfExists: true) ] - IVAR_TRIM ( input, bed_file ) + IVAR_TRIM ( input, bed_file ) } From a5686ed14e21f98f96dfdb9488523f8e427a7cd3 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Sat, 13 Feb 2021 08:19:55 +0000 Subject: [PATCH 44/45] Update software/ivar/trim/main.nf --- software/ivar/trim/main.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/software/ivar/trim/main.nf b/software/ivar/trim/main.nf index e6ac8888..756a064a 100644 --- a/software/ivar/trim/main.nf +++ b/software/ivar/trim/main.nf @@ -13,7 +13,6 @@ process IVAR_TRIM { conda (params.enable_conda ? "bioconda::ivar=1.3.1" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/ivar:1.3.1--h089eab3_0" } else { From 4af9417b6d7c9db2160fc6a23f63970a046292ba Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Sat, 13 Feb 2021 08:20:06 +0000 Subject: [PATCH 45/45] Update software/ivar/trim/main.nf --- software/ivar/trim/main.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/software/ivar/trim/main.nf b/software/ivar/trim/main.nf index 756a064a..f46070e8 100644 --- a/software/ivar/trim/main.nf +++ b/software/ivar/trim/main.nf @@ -12,7 +12,6 @@ process IVAR_TRIM { saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } conda (params.enable_conda ? "bioconda::ivar=1.3.1" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/ivar:1.3.1--h089eab3_0" } else {