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Merge pull request #35 from ewels/editor-config

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Editor config
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Phil Ewels 2020-07-11 14:20:37 +02:00 committed by GitHub
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39 changed files with 288 additions and 233 deletions
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11
.github/markdownlint.yml vendored Normal file
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@ -0,0 +1,11 @@
# Markdownlint configuration file
default: true,
line-length: false
no-multiple-blanks: 0
blanks-around-headers: false
blanks-around-lists: false
header-increment: false
no-duplicate-header:
siblings_only: true
ul-indent:
indent: 4

50
.github/workflows/lint-code.yml vendored Normal file
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@ -0,0 +1,50 @@
name: Lint Code
on: [push, pull_request]
jobs:
Markdown:
runs-on: ubuntu-latest
steps:
- uses: actions/checkout@v2
- uses: actions/setup-node@v1
with:
node-version: '10'
- name: Install markdownlint
run: npm install -g markdownlint-cli
- name: Run Markdownlint
run: markdownlint ${GITHUB_WORKSPACE} -c ${GITHUB_WORKSPACE}/.github/markdownlint.yml
EditorConfig:
runs-on: ubuntu-latest
steps:
- uses: actions/checkout@v2
- uses: actions/setup-node@v1
with:
node-version: '10'
- name: Install ECLint
run: npm install -g eclint
- name: Run ECLint check
run: eclint check $(git ls-files | grep -v test)
YAML:
runs-on: ubuntu-latest
steps:
- name: Check out repository
uses: actions/checkout@v1
- name: Install NodeJS
uses: actions/setup-node@v1
with:
node-version: '10'
- name: Install yaml-lint
run: npm install -g yaml-lint
- name: Run yaml-lint
run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yaml" -or -name "*.yml")

4
.gitmodules vendored
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@ -1,3 +1,3 @@
[submodule "test-datasets"]
path = test-datasets
url = https://github.com/nf-core/test-datasets.git
path = test-datasets
url = https://github.com/nf-core/test-datasets.git

3
README.md
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@ -5,6 +5,7 @@
A repository for hosting nextflow [`DSL2`](https://www.nextflow.io/docs/edge/dsl2.htmlhttps://www.nextflow.io/docs/edge/dsl2.html) module files containing tool-specific process definitions and their associated documentation.
## Table of contents
* [Using existing modules](#using-existing-modules)
* [Configuration and parameters](#configuration-and-parameters)
* [Offline usage](#offline-usage)
@ -20,7 +21,7 @@ The features offered by Nextflow DSL 2 can be used in various ways depending on
* *Module*: A `process`that can be used within different pipelines and is as atomic as possible i.e. cannot be split into another module. An example of this would be a module file containing the process definition for a single tool such as `FastQC`. This repository has been created to only host atomic module files that should be added to the `tools` sub-directory along with the required documentation, software and tests.
* *Sub-workflow*: A chain of multiple modules that offer a higher-level of functionality within the context of a pipeline. For example, a sub-workflow to run multiple QC tools with FastQ files as input. Sub-workflows should be shipped with the pipeline implementation and if required they should be shared amongst different pipelines directly from there. As it stands, this repository will not host sub-workflows.
* *Workflow*: What DSL 1 users would consider an end-to-end pipeline. For example, from one or more inputs to a series of outputs. This can either be implemented using a large monolithic script as with DSL 1, or by using a combination of DSL 2 individual modules and sub-workflows.
* *Workflow*: What DSL 1 users would consider an end-to-end pipeline. For example, from one or more inputs to a series of outputs. This can either be implemented using a large monolithic script as with DSL 1, or by using a combination of DSL 2 individual modules and sub-workflows.
## Using existing modules

2
test-datasets

@ -1 +1 @@
Subproject commit aae85a5c9c72238959108212481ce83bae569709
Subproject commit ddbd0c4cf7f1721c78673c4dcc91fcd7940e67f8

74
tools/bowtie2/main.nf
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@ -2,51 +2,51 @@ nextflow.preview.dsl=2
params.genome = ''
process BOWTIE2 {
// depending on the genome used one might want/need to adjust the memory settings.
// For the E. coli test data this is probably not required
// depending on the genome used one might want/need to adjust the memory settings.
// For the E. coli test data this is probably not required
// label 'bigMem'
// label 'multiCore'
// label 'bigMem'
// label 'multiCore'
input:
tuple val(name), path(reads)
val (outdir)
val (bowtie2_args)
val (verbose)
tuple val(name), path(reads)
val (outdir)
val (bowtie2_args)
val (verbose)
output:
path "*bam", emit: bam
path "*stats.txt", emit: stats
output:
path "*bam", emit: bam
path "*stats.txt", emit: stats
publishDir "$outdir/bowtie2",
mode: "copy", overwrite: true
publishDir "$outdir/bowtie2",
mode: "copy", overwrite: true
script:
if (verbose){
println ("[MODULE] BOWTIE2 ARGS: " + bowtie2_args)
}
script:
if (verbose){
println ("[MODULE] BOWTIE2 ARGS: " + bowtie2_args)
}
cores = 4
cores = 4
readString = ""
readString = ""
// Options we add are
bowtie2_options = bowtie2_args
bowtie2_options += " --no-unal " // We don't need unaligned reads in the BAM file
// single-end / paired-end distinction. Might also be handled via params.single_end
if (reads instanceof List) {
readString = "-1 " + reads[0] + " -2 " + reads[1]
}
else {
readString = "-U " + reads
}
index = params.genome["bowtie2"]
bowtie2_name = name + "_" + params.genome["name"]
// Options we add are
bowtie2_options = bowtie2_args
bowtie2_options += " --no-unal " // We don't need unaligned reads in the BAM file
"""
bowtie2 -x ${index} -p ${cores} ${bowtie2_options} ${readString} 2>${bowtie2_name}_bowtie2_stats.txt | samtools view -bS -F 4 -F 8 -F 256 -> ${bowtie2_name}_bowtie2.bam
"""
// single-end / paired-end distinction. Might also be handled via params.single_end
if (reads instanceof List) {
readString = "-1 " + reads[0] + " -2 " + reads[1]
}
else {
readString = "-U " + reads
}
index = params.genome["bowtie2"]
bowtie2_name = name + "_" + params.genome["name"]
"""
bowtie2 -x ${index} -p ${cores} ${bowtie2_options} ${readString} 2>${bowtie2_name}_bowtie2_stats.txt | samtools view -bS -F 4 -F 8 -F 256 -> ${bowtie2_name}_bowtie2.bam
"""
}

8
tools/bowtie2/meta.yml
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@ -1,4 +1,4 @@
name: Bowtie 2
name: Bowtie 2
description: Ultrafast alignment to reference genome
keywords:
- Alignment
@ -28,10 +28,10 @@ output:
- report:
type: file
description: mapping statistics report
pattern: *bowtie2_stats.txt
pattern: "*bowtie2_stats.txt"
- alignment:
type: file
description: alignment file in BAM format
pattern: *bowtie2.bam
pattern: "*bowtie2.bam"
authors:
- @FelixKrueger
- "@FelixKrueger"

2
tools/bwa/index/main.nf
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@ -13,4 +13,4 @@ process bwa_index {
"""
bwa index ${fasta}
"""
}
}

6
tools/bwa/index/meta.yml
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@ -14,12 +14,12 @@ input:
- input:
type: file
description: Input fasta file
pattern: *.{fasta,fa}
pattern: "*.{fasta,fa}"
output:
-
- index:
type: file
description: bwa indexes file
pattern: *.{fasta,fa}.{amb,ann,bwt,pac,sa}
pattern: "*.{fasta,fa}.{amb,ann,bwt,pac,sa}"
authors:
- @maxulysse
- "@maxulysse"

4
tools/bwa/mem/Dockerfile
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@ -1,9 +1,7 @@
FROM nfcore/base
LABEL authors="Jeremy Guntoro" \
description="Docker image containing all requirements for nf-core/modules/bwa/mem module"
description="Docker image containing all requirements for nf-core/modules/bwa/mem module"
COPY environment.yml /
RUN conda env create -f /environment.yml && conda clean -a
ENV PATH /opt/conda/envs/nf-core-bwa-mem/bin:$PATH

10
tools/bwa/mem/meta.yml
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@ -19,11 +19,11 @@ input:
- reads:
type: file
description: Input fastq file
pattern: *.{fastq,fq}
pattern: "*.{fastq,fq}"
- index:
type: file
description: bwa indexes file
pattern: *.{amb,ann,bwt,pac,sa}
pattern: "*.{amb,ann,bwt,pac,sa}"
- prefix:
type: val
description: bwa index prefix, equivalent to index file names without extensions. Usually the reference genome file name unless otherwise specified.
@ -32,11 +32,11 @@ output:
- bam:
type: file
description: Output bam file
pattern: *.bam
pattern: "*.bam"
- bamindex:
type: file
description: Output bam index file
pattern: *.bai
pattern: "*.bai"
authors:
- @jeremy1805
- "@jeremy1805"

28
tools/cutadapt/main.nf
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@ -13,27 +13,27 @@ process cutadapt {
forward_fq = "trimmed_1.fastq"
reverse_fq = "trimmed_2.fastq"
if (params.singleEnd) {
processing = """
cutadapt \
-j ${task.cpus} \
-q $params.cutadapt_min_quality \
--minimum-length $params.cutadapt_min_length \
--output ${forward_fq} \
${reads}
-j ${task.cpus} \
-q $params.cutadapt_min_quality \
--minimum-length $params.cutadapt_min_length \
--output ${forward_fq} \
${reads}
"""
} else {
processing = """
cutadapt \
-j ${task.cpus} \
-q $params.cutadapt_min_quality \
--minimum-length $params.cutadapt_min_length \
--pair-filter=any \
--output ${forward_fq} \
--paired-output ${reverse_fq} ${reads}
-j ${task.cpus} \
-q $params.cutadapt_min_quality \
--minimum-length $params.cutadapt_min_length \
--pair-filter=any \
--output ${forward_fq} \
--paired-output ${reverse_fq} ${reads}
"""
}

8
tools/cutadapt/meta.yml
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@ -9,10 +9,10 @@ tools:
description: |
Cutadapt finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence
from your high-throughput sequencing reads.
Cleaning your data in this way is often required: Reads from small-RNA sequencing contain the 3’
sequencing adapter because the read is longer than the molecule that is sequenced. Amplicon reads
start with a primer sequence. Poly-A tails are useful for pulling out RNA from your sample, but
sequencing adapter because the read is longer than the molecule that is sequenced. Amplicon reads
start with a primer sequence. Poly-A tails are useful for pulling out RNA from your sample, but
often you don’t want them to be in your reads.
homepage: https://cutadapt.readthedocs.io/en/stable/
documentation: https://cutadapt.readthedocs.io/en/stable/
@ -33,4 +33,4 @@ output:
type: file
description: trimmed FastQ file, or pair of files
authors:
- @piotr-faba-ardigen
- "@piotr-faba-ardigen"

54
tools/fastq_screen/main.nf
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@ -1,38 +1,38 @@
nextflow.preview.dsl=2
process FASTQ_SCREEN {
// depending on the number of genomes and the type of genome (e.g. plants!), memory needs to be ample!
// label 'bigMem'
// label 'multiCore'
// depending on the number of genomes and the type of genome (e.g. plants!), memory needs to be ample!
// label 'bigMem'
// label 'multiCore'
input:
tuple val(name), path(reads)
val (outputdir)
// fastq_screen_args are best passed in to the workflow in the following manner:
// --fastq_screen_args="--subset 200000 --force"
val (fastq_screen_args)
val (verbose)
tuple val(name), path(reads)
val (outputdir)
// fastq_screen_args are best passed in to the workflow in the following manner:
// --fastq_screen_args="--subset 200000 --force"
val (fastq_screen_args)
val (verbose)
output:
path "*png", emit: png
path "*html", emit: html
path "*txt", emit: report
output:
path "*png", emit: png
path "*html", emit: html
path "*txt", emit: report
publishDir "$outputdir",
mode: "link", overwrite: true
publishDir "$outputdir",
mode: "link", overwrite: true
script:
println(name)
println(reads)
println(outputdir)
if (verbose){
println ("[MODULE] FASTQ SCREEN ARGS: "+ fastq_screen_args)
}
println(name)
println(reads)
println(outputdir)
if (verbose){
println ("[MODULE] FASTQ SCREEN ARGS: "+ fastq_screen_args)
}
"""
module load fastq_screen
fastq_screen $fastq_screen_args $reads
"""
"""
module load fastq_screen
fastq_screen $fastq_screen_args $reads
"""
}
}

6
tools/fastq_screen/meta.yml
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@ -25,7 +25,7 @@ output:
- report:
type: file
description: FastQ Screen report
pattern: *_screen.{txt,html,png}
optional_pattern: *_screen.bisulfite_orientation.png
pattern: "*_screen.{txt,html,png}"
optional_pattern: "*_screen.bisulfite_orientation.png"
authors:
- @FelixKrueger
- "@FelixKrueger"

2
tools/fastqc/Dockerfile
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@ -1,6 +1,6 @@
FROM nfcore/base:1.7
LABEL authors="phil.ewels@scilifelab.se" \
description="Docker image for nf-core modules fastqc"
description="Docker image for nf-core modules fastqc"
# foobar
COPY environment.yml /

44
tools/fastqc/main.nf
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@ -1,37 +1,37 @@
nextflow.preview.dsl = 2
process FASTQC {
process FASTQC {
// tag "FastQC - $sample_id"
input:
tuple val(name), path(reads)
val (outputdir)
input:
tuple val(name), path(reads)
val (outputdir)
// fastqc_args are best passed into the workflow in the following manner:
// --fastqc_args="--nogroup -a custom_adapter_file.txt"
val (fastqc_args)
val (verbose)
val (fastqc_args)
val (verbose)
output:
tuple val(name), path ("*fastqc*"), emit: all
path "*.zip", emit: report // e.g. for MultiQC later
// container 'quay.io/biocontainers/fastqc:0.11.8--2'
output:
tuple val(name), path ("*fastqc*"), emit: all
path "*.zip", emit: report // e.g. for MultiQC later
publishDir "$outputdir",
mode: "copy", overwrite: true
// container 'quay.io/biocontainers/fastqc:0.11.8--2'
script:
publishDir "$outputdir",
mode: "copy", overwrite: true
if (verbose){
println ("[MODULE] FASTQC ARGS: " + fastqc_args)
}
script:
"""
module load fastqc
fastqc $fastqc_args -q -t 2 $reads
if (verbose){
println ("[MODULE] FASTQC ARGS: " + fastqc_args)
}
"""
module load fastqc
fastqc $fastqc_args -q -t 2 $reads
fastqc --version &> fastqc.version.txt
"""
"""
}

7
tools/fastqc/meta.yml
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@ -27,8 +27,7 @@ output:
- report:
type: file
description: FastQC report
pattern: *_fastqc.{zip,html}
pattern: "*_fastqc.{zip,html}"
authors:
-
- @ewels
- @FelixKrueger
- "@ewels"
- "@FelixKrueger"

2
tools/gatk/dict/main.nf
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@ -16,4 +16,4 @@ process gatk_dict {
--REFERENCE ${fasta} \
--OUTPUT ${fasta.baseName}.dict
"""
}
}

6
tools/gatk/dict/meta.yml
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@ -14,12 +14,12 @@ input:
- input:
type: file
description: Input fasta file
pattern: *.{fasta,fa}
pattern: "*.{fasta,fa}"
output:
-
- dict:
type: file
description: gatk dictionary file
pattern: *.{fasta,fa}.{dict}
pattern: "*.{fasta,fa}.{dict}"
authors:
- @maxulysse
- "@maxulysse"

8
tools/hisat2/main.nf
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@ -15,17 +15,17 @@ process HISAT2 {
output:
path "*bam", emit: bam
path "*stats.txt", emit: stats
path "*stats.txt", emit: stats
publishDir "$outdir/hisat2",
mode: "copy", overwrite: true
script:
if (verbose){
println ("[MODULE] HISAT2 ARGS: " + hisat2_args)
}
cores = 4
readString = ""
hisat_options = hisat2_args
@ -41,7 +41,7 @@ process HISAT2 {
readString = "-U "+reads
}
index = params.genome["hisat2"]
splices = ''
if (params.genome.containsKey("hisat2_splices")){
splices = " --known-splicesite-infile " + params.genome["hisat2_splices"]

8
tools/hisat2/meta.yml
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@ -1,4 +1,4 @@
name: HISAT2
name: HISAT2
description: Graph-based alignment of next generation sequencing reads to a population of genomes
keywords:
- Alignment
@ -28,10 +28,10 @@ output:
- report:
type: file
description: mapping statistics report
pattern: *hisat2_stats.txt
pattern: "*hisat2_stats.txt"
- alignment:
type: file
description: alignment file in BAM format
pattern: *hisat2.bam
pattern: "*hisat2.bam"
authors:
- @FelixKrueger
- "@FelixKrueger"

6
tools/htslib/tabix/meta.yml
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@ -15,12 +15,12 @@ input:
- input:
type: file
description: Input vcf.gz file
pattern: *.{vcf.gz}
pattern: "*.{vcf.gz}"
output:
-
- index:
type: file
description: tabix index file
pattern: *.{vcf.gz.tbi}
pattern: "*.{vcf.gz.tbi}"
authors:
- @maxulysse
- "@maxulysse"

32
tools/multiqc/main.nf
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@ -1,31 +1,31 @@
nextflow.preview.dsl=2
process MULTIQC {
// tag "FastQC - $sample_id"
input:
path (file)
val (outdir)
val (multiqc_args)
path (file)
val (outdir)
val (multiqc_args)
// multiqc_args are best passed into the workflow in the following manner:
// --multiqc_args="--exlude STAR --title custom_report_title"
val (verbose)
val (verbose)
output:
path "*html", emit: html
output:
path "*html", emit: html
publishDir "${outdir}/multiqc",
mode: "copy", overwrite: true
publishDir "${outdir}/multiqc",
mode: "copy", overwrite: true
script:
if (verbose){
println ("[MODULE] MULTIQC ARGS: " + multiqc_args)
}
if (verbose){
println ("[MODULE] MULTIQC ARGS: " + multiqc_args)
}
"""
multiqc $multiqc_args -x work .
"""
"""
multiqc $multiqc_args -x work .
"""
}

4
tools/multiqc/meta.yml
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@ -21,6 +21,6 @@ output:
- multiqc_report:
type: file
description: MultiQC report
pattern: *multiqc*.html
pattern: "*multiqc*.html"
authors:
- @FelixKrueger
- "@FelixKrueger"

2
tools/samtools/Dockerfile
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@ -1,6 +1,6 @@
FROM nfcore/base:1.7
LABEL authors="phil.ewels@scilifelab.se" \
description="Docker image for nf-core modules samtools"
description="Docker image for nf-core modules samtools"
# foobar
COPY environment.yml /

6
tools/samtools/faidx/meta.yml
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@ -16,12 +16,12 @@ input:
- input:
type: file
description: Input fasta file
pattern: *.{fasta,fa}
pattern: "*.{fasta,fa}"
output:
-
- faidx:
type: file
description: samtools index fasta file
pattern: *.fasta.fai
pattern: "*.fasta.fai"
authors:
- @maxulysse
- "@maxulysse"

6
tools/samtools/index/meta.yml
View file

@ -16,12 +16,12 @@ input:
- input:
type: file
description: Input BAM or CRAM file
pattern: *.{bam,cram}
pattern: "*.{bam,cram}"
output:
-
- index:
type: file
description: BAM or CRAM index file
pattern: *.{bai}
pattern: "*.{bai}"
authors:
- @ewels
- "@ewels"

6
tools/samtools/sort/meta.yml
View file

@ -16,12 +16,12 @@ input:
- input:
type: file
description: Input BAM or CRAM file
pattern: *.{bam,cram}
pattern: "*.{bam,cram}"
output:
-
- sorted_file:
type: file
description: Sorted BAM or CRAM file
pattern: *.{bam,cram}
pattern: "*.{bam,cram}"
authors:
- @ewels
- "@ewels"

4
tools/shovill/main.nf
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@ -3,7 +3,7 @@ process shovill {
tag { shovill }
publishDir "${params.outdir}", pattern: '*.fasta', mode: 'copy'
container "quay.io/biocontainers/shovill:1.0.9--0"
input:
@ -11,7 +11,7 @@ process shovill {
output:
path("${sample_id}.fasta")
script:
"""
shovill --R1 ${forward} --R2 ${reverse} --outdir shovill_out

2
tools/shovill/meta.yml
View file

@ -27,4 +27,4 @@ output:
description: fasta file
pattern: ${sample_id}.fasta
authors:
- @annacprice
- "@annacprice"

1
tools/tcoffee/main.nf
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@ -14,4 +14,3 @@ process tcoffee {
t_coffee -seq $fasta -outfile ${fasta}.aln
"""
}

6
tools/tcoffee/meta.yml
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@ -16,13 +16,13 @@ input:
- fasta:
type: path
description: Input fasta file
pattern: *.{fasta,fa,tfa}
pattern: "*.{fasta,fa,tfa}"
output:
-
- alignment:
type: file
description: tcoffee alignment file
pattern: *.aln
pattern: "*.aln"
authors:
- @JoseEspinosa
- "@JoseEspinosa"

2
tools/trim_galore/Dockerfile
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@ -1,6 +1,6 @@
FROM nfcore/base:1.7
LABEL authors="phil.ewels@scilifelab.se" \
description="Docker image for nf-core modules trimgalore"
description="Docker image for nf-core modules trimgalore"
# foobar
COPY environment.yml /

65
tools/trim_galore/main.nf
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@ -13,43 +13,43 @@ params.three_prime_clip_r1 = 0
params.three_prime_clip_r2 = 0
process TRIM_GALORE {
process TRIM_GALORE {
// container 'quay.io/biocontainers/trim-galore:0.6.5--0' // maybe later
// tag "$sample_id"
input:
tuple val (name), path (reads)
val (outdir)
val (trim_galore_args)
val (verbose)
input:
tuple val (name), path (reads)
val (outdir)
val (trim_galore_args)
val (verbose)
output:
tuple val(name), path ("*fq.gz"), emit: reads
path "*trimming_report.txt", optional: true, emit: report
output:
tuple val(name), path ("*fq.gz"), emit: reads
path "*trimming_report.txt", optional: true, emit: report
// Trimming reports are not generated for e.g. --hardtrim5, --clock etc
// saveAs: {filename ->
// else if (filename.indexOf("trimming_report.txt") > 0) "logs/$filename"
// else filename
// }
publishDir "${outdir}/trim_galore",
mode: "copy", overwrite: true
publishDir "${outdir}/trim_galore",
mode: "copy", overwrite: true
script:
if (verbose){
println ("[MODULE] TRIM GALORE ARGS: " + trim_galore_args)
}
if (verbose){
println ("[MODULE] TRIM GALORE ARGS: " + trim_galore_args)
}
trim_galore_args += " --gzip " // we like small files
pairedString = 0
if (reads instanceof List) {
pairedString = 1
pairedString = 0
if (reads instanceof List) {
pairedString = 1
trim_galore_args += " --paired "
}
}
if (params.clip_r1 > 0){
trim_galore_args += " --clip_r1 ${params.clip_r1} "
}
@ -62,12 +62,12 @@ process TRIM_GALORE {
if (params.three_prime_clip_r2 > 0){
trim_galore_args += " --three_prime_clip_r2 ${params.three_prime_clip_r2} "
}
if (params.trim_nextseq > 0){
trim_galore_args += " --nextseq ${params.trim_nextseq} "
}
}
// Pre-set parameters for certain bisulfite-seq applications
if (params.singlecell){
trim_galore_args += " --clip_r1 6 "
@ -77,7 +77,7 @@ process TRIM_GALORE {
}
if (params.rrbs){
trim_galore_args += " --rrbs "
}
}
if (params.pbat){
trim_galore_args += " --clip_r1 $params.pbat "
if (pairedString == 1){
@ -85,17 +85,16 @@ process TRIM_GALORE {
}
}
"""
module load trim_galore
trim_galore $trim_galore_args $reads
"""
"""
module load trim_galore
trim_galore $trim_galore_args $reads
"""
}

9
tools/trim_galore/meta.yml
View file

@ -28,14 +28,13 @@ output:
- trimmed_fastq:
type: file
description: Trimmed FastQ files
pattern: *fq.gz
pattern: "*fq.gz"
-
- report:
type: file
description: Trim Galore! trimming report
pattern: *trimming_report.txt
pattern: "*trimming_report.txt"
authors:
-
- @ewels
- @FelixKrueger
- "@ewels"
- "@FelixKrueger"

4
tools/umi_tools/Dockerfile
View file

@ -1,8 +1,8 @@
FROM nfcore/base:1.7
LABEL authors="chris.cheshire@crick.ac.uk" \
description="Docker image containing all requirements for the nf-core umi_tools module"
description="Docker image containing all requirements for the nf-core umi_tools module"
# Install conda packages
COPY environment.yml /
RUN conda env create -f /environment.yml && conda clean -a
ENV PATH /opt/conda/envs/nfcore-module-umitools/bin:$PATH
ENV PATH /opt/conda/envs/nfcore-module-umitools/bin:$PATH

2
tools/umi_tools/main.nf
View file

@ -12,7 +12,7 @@ process umitools_dedup {
input:
tuple val(sample_id), path(bam)
output:
tuple val(sample_id), path("${sample_id}.dedup.bam"), emit: dedupBam
tuple val(sample_id), path("${sample_id}.dedup.bam.bai"), emit: dedupBai

17
tools/umi_tools/umi_tools.yml
View file

@ -1,6 +1,6 @@
name: umi_tools
version: 1.0
description: Tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes.
description: Tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes.
keywords:
- UMI
- RMT
@ -8,7 +8,7 @@ keywords:
tools:
- umi_tools:
description: |
Tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes.
Tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes.
homepage: https://github.com/CGATOxford/UMI-tools
documentation: https://umi-tools.readthedocs.io/en/latest/
processes:
@ -18,7 +18,7 @@ processes:
The program will execute with the following pattern:
umi_tools dedup --log={SAMPLE_ID}.dedup.log {params.umitools_dedup_args} -I {SAMPLE_ID}.bam -S {SAMPLE_ID}.dedup.bam --output-stats={SAMPLE_ID}
description: |
Groups PCR duplicates and de-duplicates reads to yield one read per group.
Groups PCR duplicates and de-duplicates reads to yield one read per group.
Use this when you want to remove the PCR duplicates prior to any downstream analysis.
input:
- sample_id:
@ -31,16 +31,15 @@ processes:
- dedupBam:
type: tuple
description: A tuple of samples id and output bam file
pattern: [sample_id, *SAMPLE_ID.dedup.bam]
pattern: [sample_id, "*SAMPLE_ID.dedup.bam"]
- dedupBam:
type: tuple
description: A tuple of samples id and output bai file
pattern: [sample_id, *SAMPLE_ID.dedup.bam.bai]
pattern: [sample_id, "*SAMPLE_ID.dedup.bam.bai"]
- report:
type: file
description: Log file for the umi_tools operation
pattern: *SAMPLE_ID.dedup.log
pattern: "*SAMPLE_ID.dedup.log"
authors:
- @candiceh08
- @chris-cheshire
- "@candiceh08"
- "@chris-cheshire"