Gets GATK3 UG working, not sure why can't test by manually installing

This commit is contained in:
James Fellows Yates 2021-11-03 13:05:46 +01:00
parent 025e214cd6
commit f2b2f32567
4 changed files with 24 additions and 15 deletions

View file

@ -5,7 +5,7 @@ params.options = [:]
options = initOptions(params.options)
process GATK3_UNIFIEDGENOTYPER {
tag '$bam'
tag "$meta.id"
label 'process_low'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
@ -15,32 +15,37 @@ process GATK3_UNIFIEDGENOTYPER {
// Software MUST be pinned to channel (i.e. "bioconda"), version (i.e. "1.10").
// For Conda, the build (i.e. "h9402c20_2") must be EXCLUDED to support installation on different operating systems.
// TODO nf-core: See section in main README for further information regarding finding and adding container addresses to the section below.
conda (params.enable_conda ? "YOUR-TOOL-HERE" : null)
conda (params.enable_conda ? "bioconda::gatk=3.5" : null)
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
container "https://depot.galaxyproject.org/singularity/YOUR-TOOL-HERE"
container "https://depot.galaxyproject.org/singularity/gatk:3.5--hdfd78af_11"
} else {
container "quay.io/biocontainers/YOUR-TOOL-HERE"
container "quay.io/biocontainers/gatk:3.5--hdfd78af_11"
}
input:
path bam
tuple val(meta), path(bam), path(bai)
path ref
path fai
path dict
output:
path "*.vcf", emit: vcf
path "*.vcf.gz", emit: vcf
path "versions.yml", emit: versions
script:
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
"""
gatk -T UnifiedGenotyper \\
gatk3 -T UnifiedGenotyper \\
-R $ref \\
-I $bam \\
-o $vcf \\
-o ${prefix}.vcf \\
$options.args
gzip --no-name ${prefix}.vcf
cat <<-END_VERSIONS > versions.yml
${getProcessName(task.process)}:
${getSoftwareName(task.process)}: \$( samtools --version 2>&1 | sed 's/^.*samtools //; s/Using.*\$//' )
${getSoftwareName(task.process)}: \$( gatk3 --version )
END_VERSIONS
"""
}

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@ -5,9 +5,14 @@ nextflow.enable.dsl = 2
include { GATK3_UNIFIEDGENOTYPER } from '../../../../modules/gatk3/unifiedgenotyper/main.nf' addParams( options: [:] )
workflow test_gatk3_unifiedgenotyper {
input = file(params.test_data['sarscov2']['illumina']['test_single_end_bam'], checkIfExists: true)
ref = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
GATK3_UNIFIEDGENOTYPER ( input, ref )
input = [ [ id:'test' ], // meta map
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
]
ref = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
dict = file(params.test_data['sarscov2']['genome']['genome_dict'], checkIfExists: true)
GATK3_UNIFIEDGENOTYPER ( input, ref, fai, dict )
}

View file

@ -6,5 +6,4 @@
- gatk3
- gatk3/unifiedgenotyper
files:
- path: output/gatk3/test.bam
md5sum: e667c7caad0bc4b7ac383fd023c654fc
- path: output/gatk3/test.vcf.gz